##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407437
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.254211080486066	34.0	31.0	34.0	30.0	34.0
2	32.430289836220076	34.0	31.0	34.0	30.0	34.0
3	32.525907072749895	34.0	31.0	34.0	30.0	34.0
4	36.04315022936061	37.0	35.0	37.0	35.0	37.0
5	35.88187867081291	37.0	35.0	37.0	35.0	37.0
6	35.85672631597032	37.0	35.0	37.0	35.0	37.0
7	35.85122607912389	37.0	35.0	37.0	35.0	37.0
8	35.97617300343366	37.0	35.0	37.0	35.0	37.0
9	37.65164675765824	39.0	37.0	39.0	35.0	39.0
10	37.24803589266561	39.0	37.0	39.0	34.0	39.0
11	37.186887788786976	39.0	37.0	39.0	34.0	39.0
12	37.11038516384128	39.0	37.0	39.0	33.0	39.0
13	37.088052876886486	39.0	37.0	39.0	33.0	39.0
14	38.318547407329234	40.0	38.0	41.0	33.0	41.0
15	38.32236394829139	40.0	38.0	41.0	33.0	41.0
16	38.31480940611677	40.0	38.0	41.0	34.0	41.0
17	38.237999985273795	40.0	37.0	41.0	33.0	41.0
18	38.131765156330914	40.0	37.0	41.0	33.0	41.0
19	38.05055260077018	40.0	37.0	41.0	33.0	41.0
20	37.999266144213706	40.0	37.0	41.0	33.0	41.0
21	37.972972508633234	40.0	37.0	41.0	33.0	41.0
22	37.92065767222908	40.0	37.0	41.0	33.0	41.0
23	37.80903796169714	40.0	36.0	41.0	33.0	41.0
24	37.757231179298884	40.0	36.0	41.0	33.0	41.0
25	37.66978943983978	40.0	36.0	41.0	33.0	41.0
26	37.602922660435844	40.0	36.0	41.0	33.0	41.0
27	37.547476051512255	40.0	36.0	41.0	33.0	41.0
28	37.45173609662353	39.0	36.0	41.0	33.0	41.0
29	37.37199370700255	39.0	36.0	41.0	32.0	41.0
30	37.3009225966223	39.0	35.0	41.0	32.0	41.0
31	37.18822051016476	39.0	35.0	41.0	32.0	41.0
32	37.08287907087476	39.0	35.0	41.0	31.0	41.0
33	36.996772507160614	39.0	35.0	41.0	31.0	41.0
34	36.84626334868949	39.0	35.0	41.0	31.0	41.0
35	36.74121152472652	39.0	35.0	41.0	31.0	41.0
36	36.68075309802497	39.0	35.0	41.0	31.0	41.0
37	36.5903636635848	39.0	35.0	41.0	30.0	41.0
38	36.49611105520608	39.0	35.0	41.0	30.0	41.0
39	36.42003058141504	39.0	35.0	41.0	30.0	41.0
40	36.31410991147098	39.0	35.0	40.0	30.0	41.0
41	36.209701131708705	39.0	35.0	40.0	30.0	41.0
42	36.1174439238459	38.0	35.0	40.0	30.0	41.0
43	36.00461421029509	38.0	35.0	40.0	30.0	41.0
44	35.874336400474185	38.0	35.0	40.0	29.0	41.0
45	35.70649204662316	38.0	35.0	40.0	28.0	41.0
46	35.51811200259181	38.0	34.0	40.0	28.0	41.0
47	35.42214870028986	38.0	34.0	40.0	27.0	41.0
48	35.33997648716243	38.0	34.0	40.0	27.0	41.0
49	35.24086914050516	37.0	34.0	40.0	27.0	41.0
50	35.101019298689124	37.0	34.0	40.0	27.0	41.0
51	33.98859946445708	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	3.0
11	6.0
12	12.0
13	10.0
14	22.0
15	32.0
16	47.0
17	70.0
18	127.0
19	232.0
20	486.0
21	738.0
22	1041.0
23	1474.0
24	1932.0
25	2371.0
26	2955.0
27	3600.0
28	4544.0
29	5686.0
30	6997.0
31	9264.0
32	11841.0
33	16201.0
34	25155.0
35	33644.0
36	36425.0
37	51510.0
38	79480.0
39	111466.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.400945422237058	32.65928229394974	21.147073044421592	25.79269923939161
2	22.628283636488586	32.59964117151854	19.916944214688407	24.855130977304466
3	21.109766663312364	32.026791872117656	21.18437942553082	25.679062039039163
4	20.886419250092654	32.727513701504776	19.779745089424868	26.60632195897771
5	18.394500254027	34.830906373255246	17.873192665369125	28.901400707348625
6	20.118202323303972	38.373049084889196	20.014137154946653	21.49461143686018
7	82.529323551862	7.684378198347229	5.703458448790856	4.082839800999909
8	83.77147878076856	6.979974818192751	4.836085088001335	4.412461313037353
9	75.64065119269972	10.740065335254284	8.194886571420858	5.424396900625127
10	35.709815259782495	32.20866048002513	15.510864256314475	16.570660003877897
11	25.010737856404795	30.499439177099774	25.98953948708635	18.500283479409084
12	24.863230388992655	25.959596207511836	29.65734579824611	19.5198276052494
13	23.708941505067042	27.451606015163083	28.107658361906257	20.73179411786362
14	20.929125238994004	27.88529269555784	29.681398596592846	21.50418346885531
15	20.660372032976877	29.91186367462945	28.919072151031937	20.50869214136173
16	22.91151760885732	28.58282384761325	27.669308383872842	20.836350159656586
17	22.359284993753636	28.080905759663455	29.397428314070638	20.162380932512267
18	22.69332436671191	27.592241254476154	27.845531947270374	21.868902431541564
19	22.2858994151243	30.153618841685955	27.942970324246446	19.617511418943298
20	22.92869817910499	28.89624653627432	27.988376117043863	20.186679167576827
21	22.973613098466757	29.48063136141293	27.117812078922633	20.427943461197682
22	22.291299022916427	28.26154718398182	27.852649612087266	21.594504181014486
23	22.018864266131942	29.575124497775118	26.298789751544412	22.107221484548532
24	21.33507757027467	29.4546150693236	27.697779043140415	21.51252831726132
25	20.8653116923598	30.864894449939502	26.735421672553056	21.534372185147642
26	21.50369259541966	29.183407496128233	27.188497853655903	22.124402054796203
27	19.61505705176506	29.377547939926906	27.704405834521655	23.302989173786376
28	20.580605099684124	29.622739221032944	28.176626079614763	21.620029599668168
29	20.985084810657845	30.23927625620648	26.990430422372047	21.785208510763628
30	22.18649754440564	28.84298676850654	27.051053291674542	21.919462395413277
31	22.511210322086605	30.984667568237544	25.203405679896523	21.300716429779328
32	22.73136705797461	29.533891129180706	25.73649423100995	21.998247581834736
33	22.22159499505445	29.373375515723904	26.20478748861787	22.200242000603772
34	20.49887467264878	29.858603906861674	27.598131735703923	22.044389684785624
35	21.200578248907192	31.079406141317556	26.39352832462442	21.326487285150833
36	22.024754747359715	31.09953195217911	26.28479985862845	20.590913441832726
37	21.26218286508098	31.82676094709121	25.574260560528376	21.336795627299434
38	21.716977103208592	30.107231302017247	26.92440794527743	21.251383649496734
39	21.056506895544587	28.955151348552043	26.870411867356182	23.117929888547188
40	21.46614077759261	29.67133569116207	26.579569356734904	22.282954174510415
41	19.76968218399409	28.08115119638128	28.899682650323854	23.249483969300773
42	21.018218767564065	28.669708445722897	26.962696073257952	23.349376713455086
43	20.008492110436706	28.578160549974598	28.57889686012807	22.834450479460628
44	20.80493425977513	28.991721419507797	27.119775572665223	23.083568748051846
45	21.642855214425786	28.99613928042863	27.613103375491182	21.7479021296544
46	19.78808993783088	29.24550298573767	29.17727157818264	21.78913549824881
47	19.58585008234402	29.580524105567243	28.891828675353494	21.94179713673525
48	20.279208810196423	27.880138524483538	29.653909684196577	22.186742981123462
49	19.86073920630674	28.29615376119498	29.02215557251796	22.820951459980314
50	20.341058863088037	28.583069284331074	28.030345795791746	23.045526056789146
51	20.38278310511809	28.622339159182893	29.16769954618751	21.827178189511507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5952.0
1	4558.0
2	3164.0
3	2025.0
4	886.0
5	826.0
6	766.0
7	893.0
8	1020.0
9	1054.0
10	1088.0
11	1212.5
12	1337.0
13	1613.0
14	1889.0
15	1651.5
16	1414.0
17	1533.0
18	1652.0
19	1686.5
20	1721.0
21	1896.0
22	2071.0
23	2131.5
24	2192.0
25	3067.5
26	4782.0
27	5621.0
28	6796.0
29	7971.0
30	9082.0
31	10193.0
32	11678.0
33	13163.0
34	13749.0
35	14335.0
36	15719.0
37	17103.0
38	17760.0
39	18417.0
40	21508.5
41	24600.0
42	23613.5
43	22627.0
44	22398.0
45	22169.0
46	24769.5
47	27370.0
48	28674.5
49	29979.0
50	28519.5
51	27060.0
52	24416.0
53	21772.0
54	20461.5
55	19151.0
56	18331.5
57	17512.0
58	16336.0
59	15160.0
60	14694.0
61	14228.0
62	13449.0
63	12670.0
64	11428.0
65	10186.0
66	9305.5
67	8425.0
68	7211.0
69	5997.0
70	5269.0
71	4541.0
72	3708.0
73	2875.0
74	2420.0
75	1692.5
76	1420.0
77	1063.0
78	706.0
79	641.0
80	576.0
81	448.5
82	321.0
83	227.0
84	133.0
85	85.5
86	38.0
87	33.5
88	29.0
89	19.5
90	10.0
91	6.0
92	2.0
93	2.0
94	2.0
95	2.5
96	3.0
97	3.5
98	4.0
99	6.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	407437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.311848051151614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97631308669577	18.4716534818371
2	9.003804493886012	4.558058624630377
3	3.482582115792522	2.6445176802179526
4	1.9861950737076046	2.010970716225311
5	1.2898022006412135	1.6323638659335684
6	1.010238639365353	1.5342604161010787
7	0.7446976248173505	1.3194771186401202
8	0.6887948914384651	1.3947737304399919
9	0.5879712337054595	1.3394374673400673
>10	7.090593564661359	37.24727664229024
>50	0.8155729890543669	14.236039461865072
>100	0.31644631276650464	11.1048738628975
>500	0.003993013410302898	0.6395238969485898
>1k	0.001996506705151449	0.5720593056861348
>5k	9.982533525757244E-4	1.2947137289468882
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5124	1.257617742129458	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	1182	0.2901062004678024	RNA PCR Primer, Index 40 (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1082	0.2655625286854164	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	844	0.20714858984333773	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	574	0.14088067603089557	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGT	571	0.140144365877424	No Hit
AAAGGCGGGAGATTTTTCTTTTCTATTTTATGAAAGAATAGAAACTAGTGA	542	0.13302670106053208	No Hit
AATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	482	0.11830049799110047	TruSeq Adapter, Index 19 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	469	0.1151098206593903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3531834369485344	0.0
2	0.0	0.0	0.0	0.4557759849989078	0.0
3	0.0	0.0	0.0	0.5951840407228602	0.0
4	0.0	0.0	0.0	1.2262018422480039	0.0
5	0.0	0.0	0.0	1.3133318770754743	0.0
6	0.0	0.0	0.0	1.914651835743931	0.0
7	0.0	0.0	0.0	2.3407299778861517	0.0
8	0.0	0.0	0.0	2.7734349114096166	0.0
9	0.0	0.0	0.0	3.4550126768064757	0.0
10	0.0	0.0	0.0	4.321158854006877	0.0
11	0.0	0.0	0.0	5.187550467925102	0.0
12	0.0	0.0	0.0	5.5777948492650395	0.0
13	0.0	0.0	0.0	5.749600551741741	0.0
14	0.0	0.0	0.0	5.9493860400503635	0.0
15	0.0	0.0	0.0	6.119473685502299	0.0
16	0.0	0.0	0.0	6.449095197539743	0.0
17	0.0	0.0	0.0	6.828049489859782	0.0
18	0.0	0.0	0.0	7.311314387254962	0.0
19	0.0	0.0	0.0	7.541288591855919	0.0
20	0.0	0.0	0.0	7.771753669892523	0.0
21	0.0	0.0	0.0	8.020381065048094	0.0
22	0.0	0.0	0.0	8.304351347570298	0.0
23	0.0	0.0	0.0	8.593966674602454	0.0
24	0.0	0.0	0.0	8.823204569049938	0.0
25	0.0	0.0	0.0	9.043115868220118	0.0
26	0.0	0.0	0.0	9.239219805761381	0.0
27	0.0	0.0	0.0	9.443423154990834	0.0
28	0.0	0.0	0.0	9.642472333145983	0.0
29	0.0	0.0	0.0	9.862383632316162	0.0
30	0.0	0.0	0.0	10.122301116491629	0.0
31	0.0	0.0	0.0	10.342457852379631	0.0
32	0.0	0.0	0.0	10.572186620262764	0.0
33	0.0	0.0	0.0	10.8122237302945	0.0
34	0.0	0.0	0.0	11.036307453667684	0.0
35	0.0	0.0	0.0	11.301133672199628	0.0
36	0.0	0.0	0.0	11.51613623701333	0.0
37	0.0	0.0	0.0	11.74758306192123	0.0
38	0.0	0.0	0.0	11.99817395081939	0.0
39	0.0	0.0	0.0	12.256864251405739	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	20	7.0279185E-4	45.000004	15
TAATACG	40	6.7957444E-9	45.000004	1
TCCATAT	20	7.0279185E-4	45.000004	22
AATTTCG	20	7.0279185E-4	45.000004	31
TTGGACG	20	7.0279185E-4	45.000004	1
CGACGCT	20	7.0279185E-4	45.000004	23
TTCGTAC	20	7.0279185E-4	45.000004	16
CGACAAC	20	7.0279185E-4	45.000004	25
CCTAAGT	20	7.0279185E-4	45.000004	43
CATAGGA	20	7.0279185E-4	45.000004	37
CGAATTG	20	7.0279185E-4	45.000004	1
TTAGTAC	20	7.0279185E-4	45.000004	25
TAGGCAT	20	7.0279185E-4	45.000004	23
TACAACG	20	7.0279185E-4	45.000004	1
TAATCCG	20	7.0279185E-4	45.000004	1
TAGAACG	25	3.8863574E-5	45.0	1
TGTATCG	25	3.8863574E-5	45.0	29
TACGATG	25	3.8863574E-5	45.0	1
ACGGAAC	35	1.2093551E-7	45.0	42
GTATCGA	25	3.8863574E-5	45.0	30
>>END_MODULE