FastQCFastQC Report
Sat 18 Jun 2016
SRR3554175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701104
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58530.834826216937858No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14580.20795773522901023No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11040.1574659394326662No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT10260.1463406284944887No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8950.1276558114060111No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA7030.10027043063511262No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC207.031401E-445.00000427
TCGTTAA207.031401E-445.00000412
TCGCACG207.031401E-445.0000041
CGAACCG406.8084773E-945.0000041
CGAAATA207.031401E-445.00000426
GCGAAAG207.031401E-445.0000041
ACCGAAA207.031401E-445.00000415
ATTCGTT207.031401E-445.00000410
TCGATCC207.031401E-445.00000410
CGAATAT1150.045.00000413
GATTCGT207.031401E-445.0000049
TTCGCAT207.031401E-445.00000426
TTCGATC207.031401E-445.0000049
TAGTACG207.031401E-445.0000041
TGCGTAG351.2110286E-745.0000041
ATAGCGG900.045.02
TACGTAG453.8380676E-1045.01
TCGCAAC302.1642863E-644.99999616
GCGCGTA302.1642863E-644.99999613
CTTCACG302.1642863E-644.9999961