FastQCFastQC Report
Sat 18 Jun 2016
SRR3554174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420603
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72821.7313238374429094No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13000.30908005886786355No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12510.29743011818745946No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT8520.202566315504169No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7250.17237157129169312No Hit
CGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA7020.1669032317886463No Hit
TCAATTGGGTCTGGATGTAATAAAATCATGGAAAGCTTTATGTGAAGAAAA4270.10152091164352132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG302.1622072E-645.0000042
CGGGCTA351.2094642E-745.0000046
CGTAAGG302.1622072E-645.0000042
CGGTAGT351.2094642E-745.00000412
AATCGCG351.2094642E-745.0000041
TGCCGAT351.2094642E-745.00000438
TCGCCGG207.0281804E-445.02
TGTTTCG253.8865728E-545.015
CGGGTAT900.045.06
TCGCACT207.0281804E-445.033
GTTTCGT253.8865728E-545.016
TACCACG207.0281804E-445.01
CGAAACG207.0281804E-445.01
CCGGATA253.8865728E-545.032
CGACCAG253.8865728E-545.01
GTCTATC207.0281804E-445.010
TTCGTGA253.8865728E-545.018
TACGGGT950.045.04
CGACAAG406.7975634E-945.01
TTATCGT253.8865728E-545.037