##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420603
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18179138047042	33.0	31.0	34.0	30.0	34.0
2	32.361904218467295	34.0	31.0	34.0	30.0	34.0
3	32.46236237021609	34.0	31.0	34.0	30.0	34.0
4	36.01064899679746	37.0	35.0	37.0	35.0	37.0
5	35.82988233559913	37.0	35.0	37.0	35.0	37.0
6	35.801608642829464	37.0	35.0	37.0	35.0	37.0
7	35.87613973271708	37.0	35.0	37.0	35.0	37.0
8	36.00756532882552	37.0	35.0	37.0	35.0	37.0
9	37.66145510136637	39.0	38.0	39.0	35.0	39.0
10	37.1750035068699	39.0	37.0	39.0	34.0	39.0
11	37.12992536905348	39.0	37.0	39.0	34.0	39.0
12	37.10735063706155	39.0	37.0	39.0	33.0	39.0
13	37.1057862164559	39.0	37.0	39.0	33.0	39.0
14	38.34929137452657	40.0	38.0	41.0	34.0	41.0
15	38.361212354643214	40.0	38.0	41.0	34.0	41.0
16	38.33237043007301	40.0	38.0	41.0	34.0	41.0
17	38.27482447818965	40.0	38.0	41.0	34.0	41.0
18	38.14279498719695	40.0	37.0	41.0	33.0	41.0
19	38.09549622803451	40.0	37.0	41.0	34.0	41.0
20	38.01158812466863	40.0	37.0	41.0	33.0	41.0
21	37.98073717971579	40.0	37.0	41.0	33.0	41.0
22	37.9167171893686	40.0	37.0	41.0	33.0	41.0
23	37.794176456183145	40.0	37.0	41.0	33.0	41.0
24	37.73496147198189	40.0	36.0	41.0	33.0	41.0
25	37.6939584358647	40.0	36.0	41.0	33.0	41.0
26	37.576443819944224	40.0	36.0	41.0	33.0	41.0
27	37.52207901512828	39.0	36.0	41.0	33.0	41.0
28	37.445260732805046	39.0	36.0	41.0	32.0	41.0
29	37.2949384574052	39.0	36.0	41.0	32.0	41.0
30	37.25829820519587	39.0	36.0	41.0	32.0	41.0
31	37.17903819040758	39.0	35.0	41.0	31.0	41.0
32	37.11810900064907	39.0	35.0	41.0	31.0	41.0
33	37.01814537699446	39.0	35.0	41.0	31.0	41.0
34	36.90807245787595	39.0	35.0	41.0	31.0	41.0
35	36.84903578909328	39.0	35.0	41.0	31.0	41.0
36	36.747122583528885	39.0	35.0	41.0	31.0	41.0
37	36.69399885402624	39.0	35.0	41.0	31.0	41.0
38	36.644598350463504	39.0	35.0	41.0	30.0	41.0
39	36.53573797619133	39.0	35.0	41.0	30.0	41.0
40	36.48453529813149	39.0	35.0	41.0	30.0	41.0
41	36.37245811370818	39.0	35.0	40.0	30.0	41.0
42	36.2343920514119	39.0	35.0	40.0	30.0	41.0
43	36.14356055472738	39.0	35.0	40.0	30.0	41.0
44	36.07528714726238	38.0	35.0	40.0	29.0	41.0
45	35.927822673637614	38.0	35.0	40.0	29.0	41.0
46	35.80747640887012	38.0	35.0	40.0	28.0	41.0
47	35.665611039388686	38.0	34.0	40.0	28.0	41.0
48	35.56091135821666	38.0	34.0	40.0	27.0	41.0
49	35.38639762436312	38.0	34.0	40.0	27.0	41.0
50	35.25000059438473	38.0	34.0	40.0	27.0	41.0
51	34.2222951334156	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	6.0
12	3.0
13	8.0
14	12.0
15	28.0
16	46.0
17	65.0
18	113.0
19	263.0
20	441.0
21	666.0
22	1057.0
23	1568.0
24	1974.0
25	2530.0
26	3116.0
27	3698.0
28	4663.0
29	5844.0
30	7273.0
31	9516.0
32	12135.0
33	16392.0
34	23752.0
35	34168.0
36	36717.0
37	51552.0
38	84843.0
39	118111.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.98582273545362	33.88278257644382	19.0148429754424	26.116551712660158
2	21.858379517026744	32.09820186731906	20.956579006806894	25.0868396088473
3	20.532426064483612	34.33950780189395	20.284448755715008	24.84361737790743
4	20.387158436815714	33.84664398494542	19.193633901802887	26.572563676435973
5	18.59734714207935	35.72442421951341	18.261638647370564	27.416589991036677
6	20.03623369305497	37.80001569175683	20.097098689262797	22.0666519259254
7	84.44804245333486	7.411264303868494	4.2693466285309425	3.871346614265709
8	85.81298754407362	7.345881983723368	3.7141912920259723	3.1269391801770317
9	78.47732897768203	11.241955002698505	6.192775610254801	4.08794040936465
10	29.70901301227048	40.701801936743195	15.020815353195294	14.568369697791027
11	21.992234957905673	33.00261767034472	29.586807512071957	15.418339859677655
12	23.846715311112852	27.665756069262464	30.636728696656945	17.850799922967738
13	23.32769856610628	28.61177880328956	29.441064376621185	18.619458253982973
14	19.16343915759041	30.1857095646013	29.892559016459703	20.758292261348586
15	18.40286445888403	32.227539984260694	30.757032165723974	18.612563391131303
16	20.78943802112681	31.817890029315056	28.232085838664965	19.16058611089317
17	21.218583795170268	30.215904308813773	29.240875600031384	19.324636295984572
18	21.8916650618279	29.95033321207885	28.86831525214989	19.289686473943362
19	21.389053335330466	31.43272872518741	28.325523117999634	18.85269482148249
20	21.70645478039862	31.585128969598408	28.580870797402774	18.127545452600195
21	22.096846670137875	31.489314151349372	28.076357039773846	18.337482138738906
22	21.883581429519047	29.89493655537407	28.567318825590878	19.654163189516005
23	20.920678169199935	32.07704177098118	27.340270991885458	19.66200906793342
24	18.39763387327242	31.28769885141095	29.82242161848584	20.49224565683079
25	19.89286809651857	32.98740142129276	27.41040838985932	19.709322092329344
26	20.886917116615905	32.10414571460498	27.2701335939116	19.73880357486751
27	19.04789076635212	31.83714809452144	28.663609151622786	20.451351987503656
28	20.719776130935823	31.72207521106602	27.98125548319912	19.57689317479904
29	19.904993544981846	30.904677332306235	28.850959218075	20.339369904636914
30	21.039555114918343	30.51642522758991	29.636735829273686	18.807283828218058
31	21.357907575552243	32.42725325306762	26.29795793182645	19.91688123955369
32	21.184347234803365	30.555892373568422	28.409687995568266	19.850072396059943
33	20.54169846624965	31.229211394117495	28.37830448189861	19.850785657734253
34	20.367662617717897	30.82764507148071	29.529984332018554	19.274707978782843
35	21.23189801309073	29.911817081666086	30.34310264073247	18.513182264510714
36	19.30728026190969	32.392065677134966	29.673587682446396	18.62706637850895
37	21.076644721982486	31.098922261610117	28.492188595896845	19.332244420510552
38	21.112545559589446	30.194506458584463	30.022610395075645	18.670337586750453
39	20.09757419704567	30.774863707581734	29.146487305130968	19.981074790241628
40	21.8871477378906	30.89231412995152	28.57659122735691	18.643946904800966
41	20.452778510852276	30.380667755579488	29.64481946158254	19.521734271985697
42	21.4687008889618	30.534970031121983	28.294852866004284	19.70147621391193
43	20.062148867221584	31.322410919560728	29.72732006191111	18.888120151306577
44	21.297042579344417	29.79460441318773	28.229470545859158	20.67888246160869
45	21.044785700529953	30.582996317192222	28.72423639393918	19.64798158833865
46	19.149173924104204	30.7294527143173	30.271300965518556	19.850072396059943
47	19.90428028330754	30.73325677658029	29.969353523393792	19.393109416718378
48	20.385018651792784	31.082041735318107	29.788422812010374	18.74451680087874
49	20.60708078639477	31.45745512989684	28.672406045605953	19.263058038102436
50	19.18887882397415	30.46958771097686	29.559228060665284	20.782305404383706
51	19.612318504623126	30.192366673561526	29.080391723311532	21.114923098503816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8327.0
1	6222.0
2	4117.0
3	2592.5
4	1068.0
5	949.0
6	830.0
7	927.0
8	1024.0
9	1140.5
10	1257.0
11	1532.0
12	1807.0
13	1835.5
14	1864.0
15	1941.0
16	2018.0
17	1887.0
18	1756.0
19	2055.5
20	2355.0
21	2590.5
22	2826.0
23	3265.5
24	3705.0
25	4272.0
26	5281.5
27	5724.0
28	7155.5
29	8587.0
30	10631.0
31	12675.0
32	14185.0
33	15695.0
34	17352.5
35	19010.0
36	20116.5
37	21223.0
38	22779.5
39	24336.0
40	23979.5
41	23623.0
42	24354.0
43	25085.0
44	25959.0
45	26833.0
46	28067.0
47	29301.0
48	30738.5
49	32176.0
50	31134.5
51	30093.0
52	27599.0
53	25105.0
54	22080.5
55	19056.0
56	16977.5
57	14899.0
58	13814.0
59	12729.0
60	11601.0
61	10473.0
62	9565.0
63	8657.0
64	7194.0
65	5731.0
66	4726.5
67	3722.0
68	3070.0
69	2418.0
70	2004.0
71	1590.0
72	1532.5
73	1475.0
74	1330.0
75	915.5
76	646.0
77	479.0
78	312.0
79	273.0
80	234.0
81	166.5
82	99.0
83	74.0
84	49.0
85	41.5
86	34.0
87	23.0
88	12.0
89	9.5
90	7.0
91	7.0
92	7.0
93	6.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	420603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.369634807766843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.88479612596237	21.528239535553922
2	8.293825887147307	4.705856231551439
3	3.1983572318658178	2.722086799584399
4	1.9328659104328196	2.193388000454435
5	1.296802308510907	1.839490395516171
6	0.9530012317582701	1.6221778149800457
7	0.7438831788265985	1.477258588606592
8	0.609539918726098	1.3833939916012226
9	0.519702791644413	1.3269400566776046
>10	5.5555268046820085	33.12560361572841
>50	0.7371546721736997	14.649446415596955
>100	0.26950643481521364	10.541198955655874
>500	0.0025187517272449874	0.5428279198287178
>1k	0.001679167818163325	0.6076147536125753
>5k	8.395839090816625E-4	1.7344769250516558
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7282	1.7313238374429094	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1300	0.30908005886786355	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1251	0.29743011818745946	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	852	0.202566315504169	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	725	0.17237157129169312	No Hit
CGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA	702	0.1669032317886463	No Hit
TCAATTGGGTCTGGATGTAATAAAATCATGGAAAGCTTTATGTGAAGAAAA	427	0.10152091164352132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.755077828736362E-4	0.0	0.0	0.25677420275176355	0.0
2	4.755077828736362E-4	0.0	0.0	0.3742246251215517	0.0
3	4.755077828736362E-4	0.0	0.0	0.5133106516120902	0.0
4	4.755077828736362E-4	0.0	0.0	1.0881995611063164	0.0
5	4.755077828736362E-4	0.0	0.0	1.186629672161159	0.0
6	4.755077828736362E-4	0.0	0.0	1.789098033062056	0.0
7	4.755077828736362E-4	0.0	0.0	2.207544881990856	0.0
8	4.755077828736362E-4	0.0	0.0	2.625753977028219	0.0
9	4.755077828736362E-4	0.0	0.0	3.1466727531662873	0.0
10	4.755077828736362E-4	0.0	0.0	4.0838985932102245	0.0
11	4.755077828736362E-4	0.0	0.0	4.875619051694828	0.0
12	4.755077828736362E-4	0.0	0.0	5.233200904415803	0.0
13	4.755077828736362E-4	0.0	0.0	5.407236752947554	0.0
14	4.755077828736362E-4	0.0	0.0	5.578181800890626	0.0
15	4.755077828736362E-4	0.0	0.0	5.727491244712948	0.0
16	4.755077828736362E-4	0.0	0.0	6.029914194620581	0.0
17	4.755077828736362E-4	0.0	0.0	6.393915402410349	0.0
18	4.755077828736362E-4	0.0	0.0	6.860150783517949	0.0
19	4.755077828736362E-4	0.0	0.0	7.063430360696429	0.0
20	4.755077828736362E-4	0.0	0.0	7.309030130550662	0.0
21	7.132616743104542E-4	0.0	0.0	7.5736502117198405	0.0
22	7.132616743104542E-4	0.0	0.0	7.8563395886382175	0.0
23	7.132616743104542E-4	0.0	0.0	8.15044115234556	0.0
24	7.132616743104542E-4	0.0	0.0	8.351580944501109	0.0
25	7.132616743104542E-4	0.0	0.0	8.556524798919646	0.0
26	7.132616743104542E-4	0.0	0.0	8.743637111480423	0.0
27	7.132616743104542E-4	0.0	0.0	8.957140105990685	0.0
28	7.132616743104542E-4	0.0	0.0	9.14948300416307	0.0
29	7.132616743104542E-4	0.0	0.0	9.35727990527885	0.0
30	7.132616743104542E-4	0.0	0.0	9.642346821111595	0.0
31	7.132616743104542E-4	0.0	0.0	9.862744678473526	0.0
32	7.132616743104542E-4	0.0	0.0	10.076009919092352	0.0
33	7.132616743104542E-4	0.0	0.0	10.311148517723364	0.0
34	7.132616743104542E-4	0.0	0.0	10.530833113410983	0.0
35	7.132616743104542E-4	0.0	0.0	10.757650325841707	0.0
36	7.132616743104542E-4	0.0	0.0	10.984229784380997	0.0
37	7.132616743104542E-4	0.0	0.0	11.247423342201554	0.0
38	7.132616743104542E-4	0.0	0.0	11.493498619838661	0.0
39	7.132616743104542E-4	0.0	0.0	11.76097174770508	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	30	2.1622072E-6	45.000004	2
CGGGCTA	35	1.2094642E-7	45.000004	6
CGTAAGG	30	2.1622072E-6	45.000004	2
CGGTAGT	35	1.2094642E-7	45.000004	12
AATCGCG	35	1.2094642E-7	45.000004	1
TGCCGAT	35	1.2094642E-7	45.000004	38
TCGCCGG	20	7.0281804E-4	45.0	2
TGTTTCG	25	3.8865728E-5	45.0	15
CGGGTAT	90	0.0	45.0	6
TCGCACT	20	7.0281804E-4	45.0	33
GTTTCGT	25	3.8865728E-5	45.0	16
TACCACG	20	7.0281804E-4	45.0	1
CGAAACG	20	7.0281804E-4	45.0	1
CCGGATA	25	3.8865728E-5	45.0	32
CGACCAG	25	3.8865728E-5	45.0	1
GTCTATC	20	7.0281804E-4	45.0	10
TTCGTGA	25	3.8865728E-5	45.0	18
TACGGGT	95	0.0	45.0	4
CGACAAG	40	6.7975634E-9	45.0	1
TTATCGT	25	3.8865728E-5	45.0	37
>>END_MODULE