FastQCFastQC Report
Sat 18 Jun 2016
SRR3554172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733591
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42650.5813866309701182No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA24830.33847198234438536No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7770.10591732995633807No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT7470.10182785775725166No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG253.8894203E-545.0000041
TCGACGG253.8894203E-545.0000042
AATACCG453.8380676E-1045.01
AATACGG650.041.5384642
ATACCCG603.6379788E-1241.2499961
ACTACCG556.002665E-1140.909091
AGTTACG451.9266736E-840.01
AACGTTG403.4559343E-739.37530
TAACGTT403.4559343E-739.37529
TAGCCGT403.4559343E-739.37544
ATGTACG403.4559343E-739.3751
GTAGGGC1500.039.04
TATCACG356.2452E-638.5714261
TTATCGG356.2452E-638.5714262
AATTACG356.2452E-638.5714261
TAAGACG356.2452E-638.5714261
TTAATCG3350.038.28358520
TACGCGG659.094947E-1238.0769272
TCTACGG659.094947E-1238.0769272
ATGGGAT4500.037.55
Produced by FastQC (version 0.11.5)