##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	733591
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.240128354900754	34.0	31.0	34.0	30.0	34.0
2	32.41614878045123	34.0	31.0	34.0	30.0	34.0
3	32.521339547513534	34.0	31.0	34.0	30.0	34.0
4	36.047856366831105	37.0	35.0	37.0	35.0	37.0
5	35.87213310959377	37.0	35.0	37.0	35.0	37.0
6	35.843810788300296	37.0	35.0	37.0	35.0	37.0
7	35.842897472842495	37.0	35.0	37.0	35.0	37.0
8	35.95867315711343	37.0	35.0	37.0	35.0	37.0
9	37.57825682158042	39.0	37.0	39.0	35.0	39.0
10	37.23148593698669	39.0	37.0	39.0	34.0	39.0
11	37.1889199840238	39.0	37.0	39.0	34.0	39.0
12	37.092674255818295	39.0	37.0	39.0	33.0	39.0
13	37.061032646256564	39.0	37.0	39.0	33.0	39.0
14	38.29614867139864	40.0	38.0	41.0	33.0	41.0
15	38.313335359894	40.0	38.0	41.0	34.0	41.0
16	38.286729253766744	40.0	38.0	41.0	33.0	41.0
17	38.25124081402307	40.0	38.0	41.0	33.0	41.0
18	38.17273385305981	40.0	37.0	41.0	33.0	41.0
19	38.130883557731764	40.0	37.0	41.0	34.0	41.0
20	38.05991349403141	40.0	37.0	41.0	33.0	41.0
21	37.99186876611082	40.0	37.0	41.0	33.0	41.0
22	37.90989120640793	40.0	37.0	41.0	33.0	41.0
23	37.84545202980953	40.0	37.0	41.0	33.0	41.0
24	37.75550954142022	40.0	36.0	41.0	33.0	41.0
25	37.67857566409621	40.0	36.0	41.0	33.0	41.0
26	37.559592470463784	40.0	36.0	41.0	33.0	41.0
27	37.48868374884643	39.0	36.0	41.0	32.0	41.0
28	37.388773853550546	39.0	36.0	41.0	32.0	41.0
29	37.26533449837852	39.0	36.0	41.0	32.0	41.0
30	37.22439751850827	39.0	36.0	41.0	32.0	41.0
31	37.14391943194505	39.0	35.0	41.0	31.0	41.0
32	37.081118770541075	39.0	35.0	41.0	31.0	41.0
33	36.96990012145732	39.0	35.0	41.0	31.0	41.0
34	36.80546108117466	39.0	35.0	41.0	31.0	41.0
35	36.75378514730961	39.0	35.0	41.0	31.0	41.0
36	36.58587141881512	39.0	35.0	41.0	30.0	41.0
37	36.47938974169531	39.0	35.0	41.0	30.0	41.0
38	36.43512938408459	39.0	35.0	41.0	30.0	41.0
39	36.319045626241326	39.0	35.0	40.0	30.0	41.0
40	36.155206375214526	39.0	35.0	40.0	30.0	41.0
41	36.02166466055336	38.0	35.0	40.0	29.0	41.0
42	35.92764769469636	38.0	35.0	40.0	29.0	41.0
43	35.884476499848006	38.0	35.0	40.0	29.0	41.0
44	35.755382767782045	38.0	35.0	40.0	28.0	41.0
45	35.61788653350436	38.0	34.0	40.0	28.0	41.0
46	35.4619767690716	38.0	34.0	40.0	27.0	41.0
47	35.311388771127234	38.0	34.0	40.0	27.0	41.0
48	35.14621498900613	38.0	34.0	40.0	26.0	41.0
49	34.9874793992838	37.0	34.0	40.0	26.0	41.0
50	34.85443387391612	37.0	34.0	40.0	26.0	41.0
51	33.73659436934204	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	10.0
12	11.0
13	20.0
14	32.0
15	50.0
16	107.0
17	153.0
18	295.0
19	485.0
20	772.0
21	1287.0
22	1850.0
23	2568.0
24	3457.0
25	4572.0
26	5755.0
27	6968.0
28	8700.0
29	10941.0
30	13842.0
31	17471.0
32	22177.0
33	29565.0
34	45607.0
35	56062.0
36	65855.0
37	92790.0
38	144560.0
39	197569.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.805202081268718	33.36354998902658	19.19461934511192	25.63662858459278
2	23.266506813742264	31.797145821036516	20.419552584478275	24.51679478074295
3	21.950242028596314	30.4378052620602	21.303696473920755	26.308256235422732
4	22.72451543162334	30.468067356333435	19.37237507003221	27.435042142011014
5	19.961531698180593	33.38972261110074	17.637620963179756	29.011124727538917
6	21.39843591319959	37.04284812654463	19.818672802692507	21.740043157563274
7	82.42631111886595	7.6449956447121075	5.365932788161251	4.562760448260679
8	83.94037004270773	6.620855490320901	4.991746081944844	4.447028385026534
9	75.36229315790406	10.947244445474386	8.118829156846253	5.571633239775297
10	36.23585894592491	33.484734681859514	14.992822976290604	15.286583395924977
11	27.386922685801764	28.775434813131568	25.188286115832938	18.649356385233734
12	27.885701978350337	24.570503182290953	28.225809749574353	19.31798508978436
13	24.586588439607358	26.526361419374012	26.791631849354747	22.095418291663886
14	20.803417708232516	28.371394959861828	28.034149819177173	22.791037512728483
15	21.451462736047745	28.91011476422148	28.997220522062022	20.641201977668754
16	22.262677704606517	28.035104029356955	28.310598139835413	21.39162012620111
17	22.319930315393727	28.032241398817597	27.39019426356103	22.257634022227645
18	22.70338649192806	27.869344089553987	26.96270810301653	22.464561315501417
19	23.457757796919537	29.04465839957142	26.37014358136891	21.12744022214013
20	25.07991510255715	27.174406447189238	26.78031764293728	20.965360807316337
21	24.284785391314777	28.50375754337226	25.659120681687753	21.55233638362521
22	23.187034737340017	28.569325414297612	25.409253930323572	22.834385918038798
23	23.41168307680983	28.54533384406297	25.583601761744625	22.459381317382572
24	21.74072473626312	27.808138322307663	27.90846670692525	22.542670234503966
25	22.49673183013423	30.162447467321705	25.33114501132102	22.009675691223038
26	23.03817794929327	29.54807242727896	25.729732234991975	21.68401738843579
27	23.21266209645429	27.98003247040926	25.888267440576563	22.919037992559886
28	23.130327389512683	28.9646410602093	26.728926608968756	21.17610494130926
29	23.53763882054169	28.228127117153832	25.705740664757336	22.528493397547134
30	22.9480732451734	28.848363734015276	25.862094818502406	22.341468202308917
31	23.39396203061379	29.48536718689297	25.495814425204234	21.62485635728901
32	24.40774218876731	27.877931981172072	25.793800632777664	21.920525197282956
33	22.693026495690376	29.132036788891902	26.01422318430842	22.160713531109298
34	22.565843910298792	28.89266634950538	27.53509789514866	21.006391845047172
35	24.937328838549	27.018597556404046	27.048314387717408	20.99575921732955
36	23.14300475332985	29.98441911092148	26.317389390000695	20.55518674574797
37	22.246592447290112	31.307772314545844	26.58047876814192	19.865156470022125
38	23.29431522469605	27.778148859514364	28.096991375303134	20.830544540486457
39	23.65555193561535	27.654101536142072	27.22320748209833	21.46713904614424
40	24.25997592664032	28.324502345312307	27.66091732314055	19.75460440490682
41	20.637794084169517	28.824917426740516	29.832836008075343	20.704452481014627
42	22.131269331275874	28.651114858279342	27.57953682637873	21.638078984066052
43	22.513362350410514	27.827904104603245	27.75102202726042	21.90771151772582
44	23.02100216605711	28.078725066147214	26.697165041555852	22.203107726239825
45	22.571978118597418	27.762745180897802	27.222116956178578	22.4431597443262
46	21.1669717867313	29.692567111646678	27.178495919388325	21.961965182233698
47	22.796762773807203	28.08295085408627	27.902605130106554	21.21768124199997
48	22.31025189785589	27.036727549819993	28.824508479520606	21.82851207280351
49	20.926783452904957	29.408485109550142	27.377107952523954	22.287623485020944
50	21.08790865754896	27.67577573879723	28.6821948469924	22.55412075666141
51	21.65457318860237	27.05322175435631	28.2909686732798	23.001236383761523
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5299.0
1	4160.5
2	3022.0
3	2072.5
4	1123.0
5	1127.5
6	1132.0
7	1306.0
8	1480.0
9	1670.5
10	1861.0
11	1983.0
12	2105.0
13	2346.0
14	2587.0
15	2639.5
16	2692.0
17	2662.0
18	2632.0
19	2922.0
20	3212.0
21	3483.0
22	3754.0
23	5207.0
24	6660.0
25	6630.5
26	7680.5
27	8760.0
28	11058.5
29	13357.0
30	15958.5
31	18560.0
32	20724.5
33	22889.0
34	23949.5
35	25010.0
36	26530.5
37	28051.0
38	30542.5
39	33034.0
40	34111.5
41	35189.0
42	36743.5
43	38298.0
44	41050.5
45	43803.0
46	46162.0
47	48521.0
48	48822.0
49	49123.0
50	48265.5
51	47408.0
52	45102.0
53	42796.0
54	39802.0
55	36808.0
56	34602.5
57	32397.0
58	32316.5
59	32236.0
60	31284.0
61	30332.0
62	28499.5
63	26667.0
64	23201.0
65	19735.0
66	17871.5
67	16008.0
68	14050.5
69	12093.0
70	10800.0
71	9507.0
72	8015.0
73	6523.0
74	5777.5
75	4061.5
76	3091.0
77	2520.0
78	1949.0
79	1512.0
80	1075.0
81	817.5
82	560.0
83	445.5
84	331.0
85	236.0
86	141.0
87	101.5
88	62.0
89	51.5
90	41.0
91	31.0
92	21.0
93	13.5
94	6.0
95	7.0
96	8.0
97	7.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	733591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.663147635135253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.15942520773159	20.5148312805825
2	7.945363468538899	4.395875252900038
3	3.089968569383207	2.56434770168326
4	1.8755265018281744	2.075318660547263
5	1.331008759135361	1.8409945903809837
6	1.0636860198533458	1.7654942042779505
7	0.8674292311837443	1.6797076019687454
8	0.7864596477483619	1.7404759475791522
9	0.6566357224490147	1.6348149839349715
>10	7.368898786143635	41.69181203318712
>50	0.7287694177843955	13.569458220478328
>100	0.12088357439751936	4.713834982901281
>500	0.0049542448523573505	0.8882210938852507
>1k	9.908489704714702E-4	0.9248134456932064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4265	0.5813866309701182	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2483	0.33847198234438536	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	777	0.10591732995633807	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	747	0.10182785775725166	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3631573996954706E-4	0.0	0.0	0.10182785775725166	0.0
2	1.3631573996954706E-4	0.0	0.0	0.1789825665800153	0.0
3	2.726314799390941E-4	0.0	0.0	0.27685726787815007	0.0
4	2.726314799390941E-4	0.0	0.0	0.7027076395430151	0.0
5	2.726314799390941E-4	0.0	0.0	0.8004460251011803	0.0
6	2.726314799390941E-4	0.0	0.0	1.443720002017473	0.0
7	2.726314799390941E-4	0.0	0.0	1.8645266913034648	0.0
8	2.726314799390941E-4	0.0	0.0	2.2676123343934154	0.0
9	2.726314799390941E-4	0.0	0.0	2.775933728739856	0.0
10	2.726314799390941E-4	0.0	0.0	3.6204097378512006	0.0
11	2.726314799390941E-4	0.0	0.0	4.290128968321585	0.0
12	2.726314799390941E-4	0.0	0.0	4.598202540652761	0.0
13	2.726314799390941E-4	0.0	0.0	4.745832487039781	0.0
14	2.726314799390941E-4	0.0	0.0	4.910638216662964	0.0
15	2.726314799390941E-4	0.0	0.0	5.036185013174916	0.0
16	2.726314799390941E-4	0.0	0.0	5.255653354525887	0.0
17	2.726314799390941E-4	0.0	0.0	5.495705372612259	0.0
18	2.726314799390941E-4	0.0	0.0	5.807868417142522	0.0
19	2.726314799390941E-4	0.0	0.0	5.953998890389877	0.0
20	4.089472199086412E-4	0.0	0.0	6.128755669030836	0.0
21	4.089472199086412E-4	0.0	0.0	6.318643494808415	0.0
22	4.089472199086412E-4	0.0	0.0	6.529251313061366	0.0
23	4.089472199086412E-4	0.0	0.0	6.727727030457026	0.0
24	4.089472199086412E-4	0.0	0.0	6.898666968378838	0.0
25	4.089472199086412E-4	0.0	0.0	7.0483416508654	0.0
26	4.089472199086412E-4	0.0	0.0	7.189837388953791	0.0
27	4.089472199086412E-4	0.0	0.0	7.340057334400231	0.0
28	4.089472199086412E-4	0.0	0.0	7.488505175227068	0.0
29	4.089472199086412E-4	0.0	0.0	7.658081955749185	0.0
30	4.089472199086412E-4	0.0	0.0	7.866372406422652	0.0
31	5.452629598781882E-4	0.0	0.0	8.037721291564374	0.0
32	5.452629598781882E-4	0.0	0.0	8.211932807245454	0.0
33	5.452629598781882E-4	0.0	0.0	8.392960109925014	0.0
34	5.452629598781882E-4	0.0	0.0	8.558311102508073	0.0
35	5.452629598781882E-4	0.0	0.0	8.741928404247053	0.0
36	5.452629598781882E-4	0.0	0.0	8.91723044584789	0.0
37	5.452629598781882E-4	0.0	0.0	9.09185090874888	0.0
38	5.452629598781882E-4	0.0	0.0	9.285146628025698	0.0
39	5.452629598781882E-4	0.0	0.0	9.47408024362349	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	25	3.8894203E-5	45.000004	1
TCGACGG	25	3.8894203E-5	45.000004	2
AATACCG	45	3.8380676E-10	45.0	1
AATACGG	65	0.0	41.538464	2
ATACCCG	60	3.6379788E-12	41.249996	1
ACTACCG	55	6.002665E-11	40.90909	1
AGTTACG	45	1.9266736E-8	40.0	1
AACGTTG	40	3.4559343E-7	39.375	30
TAACGTT	40	3.4559343E-7	39.375	29
TAGCCGT	40	3.4559343E-7	39.375	44
ATGTACG	40	3.4559343E-7	39.375	1
GTAGGGC	150	0.0	39.0	4
TATCACG	35	6.2452E-6	38.571426	1
TTATCGG	35	6.2452E-6	38.571426	2
AATTACG	35	6.2452E-6	38.571426	1
TAAGACG	35	6.2452E-6	38.571426	1
TTAATCG	335	0.0	38.283585	20
TACGCGG	65	9.094947E-12	38.076927	2
TCTACGG	65	9.094947E-12	38.076927	2
ATGGGAT	450	0.0	37.5	5
>>END_MODULE