##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554171_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	716996
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31366702185228	34.0	31.0	34.0	30.0	34.0
2	32.47956334484432	34.0	31.0	34.0	30.0	34.0
3	32.57398507104642	34.0	31.0	34.0	31.0	34.0
4	36.08542864953222	37.0	35.0	37.0	35.0	37.0
5	35.91940680282735	37.0	35.0	37.0	35.0	37.0
6	35.89110957383305	37.0	35.0	37.0	35.0	37.0
7	35.86687094488672	37.0	35.0	37.0	35.0	37.0
8	35.98601247426764	37.0	35.0	37.0	35.0	37.0
9	37.60783463227131	39.0	37.0	39.0	35.0	39.0
10	37.28168079040887	39.0	37.0	39.0	34.0	39.0
11	37.26016602603083	39.0	37.0	39.0	34.0	39.0
12	37.1826983693075	39.0	37.0	39.0	34.0	39.0
13	37.13695613364649	39.0	37.0	39.0	33.0	39.0
14	38.38664232436443	40.0	38.0	41.0	34.0	41.0
15	38.3970384772021	40.0	38.0	41.0	34.0	41.0
16	38.380773393435945	40.0	38.0	41.0	34.0	41.0
17	38.34424041417246	40.0	38.0	41.0	34.0	41.0
18	38.27649247694548	40.0	37.0	41.0	34.0	41.0
19	38.23951319114751	40.0	37.0	41.0	34.0	41.0
20	38.162109690988515	40.0	37.0	41.0	34.0	41.0
21	38.09003955391662	40.0	37.0	41.0	33.0	41.0
22	38.030036429770874	40.0	37.0	41.0	33.0	41.0
23	37.93771652840462	40.0	37.0	41.0	33.0	41.0
24	37.886105361815126	40.0	37.0	41.0	33.0	41.0
25	37.802824562480126	40.0	37.0	41.0	33.0	41.0
26	37.71011553760412	40.0	36.0	41.0	33.0	41.0
27	37.65950437659345	40.0	36.0	41.0	33.0	41.0
28	37.529910906058056	40.0	36.0	41.0	33.0	41.0
29	37.446172642525205	40.0	36.0	41.0	32.0	41.0
30	37.393431762520294	40.0	36.0	41.0	32.0	41.0
31	37.286862130332665	39.0	36.0	41.0	32.0	41.0
32	37.23303477285787	39.0	35.0	41.0	32.0	41.0
33	37.14528393463841	39.0	35.0	41.0	31.0	41.0
34	36.99577124558575	39.0	35.0	41.0	31.0	41.0
35	36.90701900707954	39.0	35.0	41.0	31.0	41.0
36	36.77164168279879	39.0	35.0	41.0	31.0	41.0
37	36.67407489023649	39.0	35.0	41.0	30.0	41.0
38	36.6228082165033	39.0	35.0	41.0	30.0	41.0
39	36.5207546485615	39.0	35.0	41.0	30.0	41.0
40	36.34574530401843	39.0	35.0	41.0	30.0	41.0
41	36.213408443003864	39.0	35.0	40.0	30.0	41.0
42	36.131045361480396	38.0	35.0	40.0	30.0	41.0
43	36.082979542424226	38.0	35.0	40.0	30.0	41.0
44	35.97397335549989	38.0	35.0	40.0	29.0	41.0
45	35.8274313943174	38.0	35.0	40.0	29.0	41.0
46	35.64421558837148	38.0	34.0	40.0	28.0	41.0
47	35.520538468833855	38.0	34.0	40.0	28.0	41.0
48	35.368566909717764	38.0	34.0	40.0	27.0	41.0
49	35.24068335109261	38.0	34.0	40.0	27.0	41.0
50	35.13299795256877	37.0	34.0	40.0	27.0	41.0
51	34.04561392253234	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	7.0
12	11.0
13	18.0
14	24.0
15	50.0
16	86.0
17	130.0
18	229.0
19	434.0
20	690.0
21	1089.0
22	1683.0
23	2335.0
24	3200.0
25	3981.0
26	5024.0
27	6204.0
28	7759.0
29	9937.0
30	12711.0
31	16049.0
32	20462.0
33	27799.0
34	43528.0
35	53994.0
36	63068.0
37	89841.0
38	141614.0
39	204956.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.81922911703831	33.31483020825778	19.111403689839275	25.75453698486463
2	23.322026901126367	31.628349391070522	20.370824941840677	24.67879876596243
3	21.97892317390892	30.35623629699468	21.197328855391103	26.46751167370529
4	22.652427628606016	30.399472242522975	19.392297864981114	27.555802263889895
5	19.88449029004346	33.40172051169044	17.625342400794423	29.088446797471672
6	21.409603400855794	36.95766782520404	19.852551478669337	21.780177295270825
7	82.46433731847877	7.533793772908077	5.388732991536912	4.613135917076246
8	83.9753638792964	6.560566586145529	4.965578608527802	4.498490926030271
9	75.35174533749142	10.866727289970935	8.167688522669582	5.613838849868061
10	36.09294891463829	33.46964278740746	14.985997132480517	15.451411165473727
11	27.36863246099002	28.635864077344923	25.30906727513124	18.686436186533818
12	28.013963815697718	24.498881444247946	28.107688187939683	19.379466552114657
13	24.520359946220065	26.509073969729258	27.012563528945766	21.958002555104912
14	20.943910426278528	28.301413118064815	27.988859073132904	22.76581738252375
15	21.44991045975152	28.849533330729876	28.99500136681376	20.70555484270484
16	22.301937528242835	27.890671635546084	28.42958677593738	21.377804060273697
17	22.30612165200364	28.026795128564174	27.4556622352147	22.211420984217487
18	22.7274629147164	27.863335360308845	26.973511707178282	22.43569001779647
19	23.507383583729897	29.06083158065038	26.274902509916377	21.156882325703354
20	25.095537492538313	27.089411935352498	26.868769142366205	20.94628142974298
21	24.331516493815865	28.548973773912266	25.664578324007387	21.454931408264482
22	23.31881907290975	28.37156692645426	25.450351187454324	22.85926281318166
23	23.408777733766996	28.591791307064472	25.50739474139326	22.492036217775276
24	21.78798765962432	27.794994672215743	27.888021690497578	22.52899597766236
25	22.512538424203203	30.243404426245057	25.224408504371016	22.019648645180727
26	22.98116028541303	29.59123900272805	25.698748668053934	21.728852043804984
27	23.144620053668362	28.008524454808676	25.9171599283678	22.929695563155164
28	23.135833393770678	28.840607200040168	26.871698028998768	21.15186137719039
29	23.459126689688645	28.144926889410822	25.71102209775229	22.684924323148245
30	22.934716511668128	28.800858024312546	25.924830821929273	22.339594642090056
31	23.380046750609488	29.323176140452667	25.603490117099675	21.69328699183817
32	24.476426646731642	27.897505704355396	25.747423974471268	21.878643674441697
33	22.660795876127622	29.13851681180927	25.95858275359974	22.242104558463367
34	22.742107347879205	28.8011369658966	27.39610820701929	21.060647479204906
35	24.850208369363287	27.043665515567728	27.148129138795753	20.95799697627323
36	23.086321262601185	29.9311293228972	26.359003397508495	20.62354601699312
37	22.201518557983587	31.209658073406267	26.688293937483614	19.900529431126536
38	23.29971157440209	27.62149300693449	28.177423583953047	20.901371834710375
39	23.664706637136053	27.52525816043604	27.329162226846453	21.480872975581452
40	24.29706720818526	28.195136374540443	27.80824439745828	19.69955201981601
41	20.724941282796557	28.715222958008134	29.868925349653274	20.690910409542035
42	22.139035643155612	28.60099637933824	27.618424649509898	21.641543327996253
43	22.468605124714784	27.741995771245588	27.74910878163895	22.040290322400683
44	23.1614960195036	27.94381000730827	26.75021896914348	22.144475004044654
45	22.612399511294345	27.68816004552327	27.224559132826403	22.474881310355986
46	21.15897438758375	29.560834370066218	27.242690335789877	22.037500906560147
47	22.660795876127622	28.040463266182797	28.100993589922396	21.197747267767184
48	22.35647060792529	26.938504538379572	28.923173908919992	21.781850944775144
49	20.909600611439952	29.349675590937746	27.38439266048904	22.35633113713326
50	21.116575266807626	27.594435673281303	28.625682709526973	22.6633063503841
51	21.750330545777103	27.000987453207546	28.212151811167708	23.036530189847642
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4965.0
1	3884.5
2	2804.0
3	1950.0
4	1096.0
5	1116.0
6	1136.0
7	1265.5
8	1395.0
9	1622.0
10	1849.0
11	1968.5
12	2088.0
13	2285.0
14	2482.0
15	2547.5
16	2613.0
17	2545.0
18	2477.0
19	2830.0
20	3183.0
21	3301.0
22	3419.0
23	5001.5
24	6584.0
25	6466.0
26	7424.5
27	8501.0
28	10602.5
29	12704.0
30	15465.5
31	18227.0
32	20385.0
33	22543.0
34	23563.0
35	24583.0
36	26192.0
37	27801.0
38	29896.0
39	31991.0
40	33233.5
41	34476.0
42	35801.5
43	37127.0
44	40078.5
45	43030.0
46	45283.0
47	47536.0
48	47910.5
49	48285.0
50	47476.0
51	46667.0
52	44341.5
53	42016.0
54	39048.5
55	36081.0
56	33745.5
57	31410.0
58	31240.5
59	31071.0
60	30478.0
61	29885.0
62	27886.5
63	25888.0
64	22526.5
65	19165.0
66	17419.0
67	15673.0
68	13715.0
69	11757.0
70	10635.5
71	9514.0
72	7966.0
73	6418.0
74	5767.5
75	4124.0
76	3131.0
77	2521.5
78	1912.0
79	1466.5
80	1021.0
81	759.0
82	497.0
83	398.5
84	300.0
85	212.5
86	125.0
87	88.0
88	51.0
89	39.5
90	28.0
91	19.0
92	10.0
93	8.5
94	7.0
95	5.5
96	4.0
97	3.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	716996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.253800748921446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.37961644084898	19.998734455111784
2	8.085751160046815	4.407349020425527
3	3.1701561562680323	2.591964126776869
4	1.994137551070603	2.173913099312815
5	1.3838161163021576	1.8857124353422656
6	1.1143228229379172	1.82217193117954
7	0.9032537895074642	1.7231969163461263
8	0.7949970450923718	1.7333352850343053
9	0.7200872779041023	1.7662603674448465
>10	7.601424562393627	42.22667967760857
>50	0.7288208246789851	13.37034813449248
>100	0.11743544030154525	4.516442311872445
>500	0.005150677206208125	0.8919461195262484
>1k	0.001030135441241625	0.8919461195262484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4031	0.5622067626597638	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2323	0.32399064987810255	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	746	0.10404521085194338	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	726	0.10125579501140872	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10474256481207705	0.0
2	0.0	0.0	0.0	0.1916328682447322	0.0
3	1.3947079202673377E-4	0.0	0.0	0.2909360721677666	0.0
4	1.3947079202673377E-4	0.0	0.0	0.7214824071542938	0.0
5	1.3947079202673377E-4	0.0	0.0	0.8262249719663708	0.0
6	1.3947079202673377E-4	0.0	0.0	1.4739273301385223	0.0
7	1.3947079202673377E-4	0.0	0.0	1.8987553626519533	0.0
8	1.3947079202673377E-4	0.0	0.0	2.3022443639852943	0.0
9	1.3947079202673377E-4	0.0	0.0	2.8002945623127604	0.0
10	1.3947079202673377E-4	0.0	0.0	3.6715686001037664	0.0
11	1.3947079202673377E-4	0.0	0.0	4.361949020636098	0.0
12	1.3947079202673377E-4	0.0	0.0	4.687473849226495	0.0
13	1.3947079202673377E-4	0.0	0.0	4.843262723920357	0.0
14	1.3947079202673377E-4	0.0	0.0	4.999609481782326	0.0
15	1.3947079202673377E-4	0.0	0.0	5.12067012926153	0.0
16	1.3947079202673377E-4	0.0	0.0	5.343683925712277	0.0
17	1.3947079202673377E-4	0.0	0.0	5.592360347895943	0.0
18	1.3947079202673377E-4	0.0	0.0	5.908261691836495	0.0
19	1.3947079202673377E-4	0.0	0.0	6.064608449698464	0.0
20	1.3947079202673377E-4	0.0	0.0	6.251638781806314	0.0
21	1.3947079202673377E-4	0.0	0.0	6.448013656979955	0.0
22	1.3947079202673377E-4	0.0	0.0	6.667540683630034	0.0
23	1.3947079202673377E-4	0.0	0.0	6.8727022187013596	0.0
24	1.3947079202673377E-4	0.0	0.0	7.046343354774643	0.0
25	1.3947079202673377E-4	0.0	0.0	7.197669164123649	0.0
26	1.3947079202673377E-4	0.0	0.0	7.347879207136441	0.0
27	1.3947079202673377E-4	0.0	0.0	7.503947023414357	0.0
28	1.3947079202673377E-4	0.0	0.0	7.654157066427149	0.0
29	1.3947079202673377E-4	0.0	0.0	7.824032491115711	0.0
30	1.3947079202673377E-4	0.0	0.0	8.049138349446858	0.0
31	1.3947079202673377E-4	0.0	0.0	8.224034722648383	0.0
32	1.3947079202673377E-4	0.0	0.0	8.39711797555356	0.0
33	1.3947079202673377E-4	0.0	0.0	8.578848417564394	0.0
34	1.3947079202673377E-4	0.0	0.0	8.742866068987832	0.0
35	1.3947079202673377E-4	0.0	0.0	8.925014923374746	0.0
36	1.3947079202673377E-4	0.0	0.0	9.104513832713153	0.0
37	1.3947079202673377E-4	0.0	0.0	9.294054639077485	0.0
38	1.3947079202673377E-4	0.0	0.0	9.48596644890627	0.0
39	1.3947079202673377E-4	0.0	0.0	9.669788952797505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATACG	55	1.8189894E-12	45.000004	1
GATCCAT	20	7.031509E-4	45.0	9
TTAAGCG	35	1.211065E-7	45.0	1
TTACGTC	20	7.031509E-4	45.0	20
GTACGAG	20	7.031509E-4	45.0	1
ATAGCGA	25	3.8893333E-5	44.999996	41
TACGCGA	25	3.8893333E-5	44.999996	30
TGCGACG	25	3.8893333E-5	44.999996	1
GTATACG	25	3.8893333E-5	44.999996	1
TATACGG	95	0.0	42.63158	2
TGACCGG	55	6.002665E-11	40.909092	2
TTACGGG	275	0.0	40.909092	3
TACGGGA	365	0.0	40.684933	4
AATACCG	50	1.0804797E-9	40.499996	1
ATACCCG	50	1.0804797E-9	40.499996	1
CGCATAG	45	1.9264917E-8	40.0	1
TACGCGG	45	1.9264917E-8	40.0	2
TTACCGG	45	1.9264917E-8	40.0	2
ATGTACG	40	3.4557888E-7	39.375	1
TATACCG	40	3.4557888E-7	39.375	1
>>END_MODULE