FastQCFastQC Report
Sat 18 Jun 2016
SRR3554169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431095
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51811.2018232640137325No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11390.26421090478896764No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8070.18719771744047134No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT8060.18696575000869878TruSeq Adapter, Index 1 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4990.115751748454517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT351.2095734E-745.00000423
CGAACTT207.028374E-445.00000439
CCGATTT406.7975634E-945.00000418
TTGTCGT207.028374E-445.0000045
CACGAAA207.028374E-445.0000041
AAGCGCG207.028374E-445.0000041
CGTTCTA207.028374E-445.00000442
CTGACGT207.028374E-445.00000430
ACGATAG207.028374E-445.0000041
TTACGTG207.028374E-445.0000041
ATCGGCG207.028374E-445.0000041
CGGTAAC351.2095734E-745.00000426
TAATGCC207.028374E-445.00000435
AATCGCA351.2095734E-745.00000425
CTTAACG253.886735E-545.01
GCCCATC253.886735E-545.031
CTACTGA253.886735E-545.041
ACGCATG253.886735E-545.01
CGACGAG253.886735E-545.01
TAGCGTC253.886735E-545.010