Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554169_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 431095 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 5181 | 1.2018232640137325 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1139 | 0.26421090478896764 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 807 | 0.18719771744047134 | No Hit |
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT | 806 | 0.18696575000869878 | TruSeq Adapter, Index 1 (95% over 24bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 499 | 0.115751748454517 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AACCGGT | 35 | 1.2095734E-7 | 45.000004 | 23 |
CGAACTT | 20 | 7.028374E-4 | 45.000004 | 39 |
CCGATTT | 40 | 6.7975634E-9 | 45.000004 | 18 |
TTGTCGT | 20 | 7.028374E-4 | 45.000004 | 5 |
CACGAAA | 20 | 7.028374E-4 | 45.000004 | 1 |
AAGCGCG | 20 | 7.028374E-4 | 45.000004 | 1 |
CGTTCTA | 20 | 7.028374E-4 | 45.000004 | 42 |
CTGACGT | 20 | 7.028374E-4 | 45.000004 | 30 |
ACGATAG | 20 | 7.028374E-4 | 45.000004 | 1 |
TTACGTG | 20 | 7.028374E-4 | 45.000004 | 1 |
ATCGGCG | 20 | 7.028374E-4 | 45.000004 | 1 |
CGGTAAC | 35 | 1.2095734E-7 | 45.000004 | 26 |
TAATGCC | 20 | 7.028374E-4 | 45.000004 | 35 |
AATCGCA | 35 | 1.2095734E-7 | 45.000004 | 25 |
CTTAACG | 25 | 3.886735E-5 | 45.0 | 1 |
GCCCATC | 25 | 3.886735E-5 | 45.0 | 31 |
CTACTGA | 25 | 3.886735E-5 | 45.0 | 41 |
ACGCATG | 25 | 3.886735E-5 | 45.0 | 1 |
CGACGAG | 25 | 3.886735E-5 | 45.0 | 1 |
TAGCGTC | 25 | 3.886735E-5 | 45.0 | 10 |