##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431095
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.388807571416976	34.0	31.0	34.0	30.0	34.0
2	32.55951240445842	34.0	31.0	34.0	31.0	34.0
3	32.643241048956725	34.0	31.0	34.0	31.0	34.0
4	36.14349737296884	37.0	35.0	37.0	35.0	37.0
5	35.99595680766421	37.0	35.0	37.0	35.0	37.0
6	35.984063837437226	37.0	35.0	37.0	35.0	37.0
7	35.98138229392593	37.0	35.0	37.0	35.0	37.0
8	36.08998712580754	37.0	36.0	37.0	35.0	37.0
9	37.730163884990546	39.0	38.0	39.0	35.0	39.0
10	37.368424593187115	39.0	37.0	39.0	35.0	39.0
11	37.3438592421624	39.0	37.0	39.0	34.0	39.0
12	37.32396571521358	39.0	37.0	39.0	34.0	39.0
13	37.291529709228826	39.0	37.0	39.0	34.0	39.0
14	38.57545784571846	40.0	38.0	41.0	34.0	41.0
15	38.61025064081003	40.0	38.0	41.0	34.0	41.0
16	38.60023892645473	40.0	38.0	41.0	34.0	41.0
17	38.51220728609703	40.0	38.0	41.0	34.0	41.0
18	38.4148528746564	40.0	38.0	41.0	34.0	41.0
19	38.380419629084074	40.0	38.0	41.0	34.0	41.0
20	38.27973416532319	40.0	37.0	41.0	34.0	41.0
21	38.21153342070773	40.0	37.0	41.0	34.0	41.0
22	38.196056553659865	40.0	37.0	41.0	34.0	41.0
23	38.06881081896102	40.0	37.0	41.0	33.0	41.0
24	38.063853674944035	40.0	37.0	41.0	33.0	41.0
25	37.983611500945266	40.0	37.0	41.0	33.0	41.0
26	37.92376158387363	40.0	37.0	41.0	33.0	41.0
27	37.87301870817337	40.0	37.0	41.0	33.0	41.0
28	37.75215207784827	40.0	37.0	41.0	33.0	41.0
29	37.681367216042865	40.0	36.0	41.0	33.0	41.0
30	37.60714923624723	40.0	36.0	41.0	33.0	41.0
31	37.483334299864296	40.0	36.0	41.0	33.0	41.0
32	37.43093981604983	40.0	36.0	41.0	33.0	41.0
33	37.34793954928728	40.0	36.0	41.0	32.0	41.0
34	37.243517090200534	40.0	36.0	41.0	32.0	41.0
35	37.12420696134263	39.0	35.0	41.0	31.0	41.0
36	37.021445389067374	39.0	35.0	41.0	31.0	41.0
37	36.96626033704868	39.0	35.0	41.0	31.0	41.0
38	36.88500678504738	39.0	35.0	41.0	31.0	41.0
39	36.781563228522714	39.0	35.0	41.0	31.0	41.0
40	36.66859508924947	39.0	35.0	41.0	31.0	41.0
41	36.59286236212436	39.0	35.0	41.0	30.0	41.0
42	36.526232037022005	39.0	35.0	41.0	30.0	41.0
43	36.44646539625837	39.0	35.0	41.0	30.0	41.0
44	36.38733689789954	39.0	35.0	41.0	30.0	41.0
45	36.21710759809323	39.0	35.0	40.0	30.0	41.0
46	36.019415674039365	38.0	35.0	40.0	29.0	41.0
47	35.90809450353171	38.0	35.0	40.0	29.0	41.0
48	35.85341746018859	38.0	35.0	40.0	29.0	41.0
49	35.72624131571927	38.0	35.0	40.0	28.0	41.0
50	35.61471601387165	38.0	34.0	40.0	28.0	41.0
51	34.59530729885524	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	3.0
11	2.0
12	2.0
13	1.0
14	6.0
15	16.0
16	34.0
17	68.0
18	124.0
19	229.0
20	419.0
21	783.0
22	1046.0
23	1432.0
24	1817.0
25	2277.0
26	2645.0
27	3326.0
28	3958.0
29	4877.0
30	6214.0
31	8456.0
32	10747.0
33	14964.0
34	23250.0
35	31822.0
36	35793.0
37	53545.0
38	88911.0
39	134245.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.559053108943505	32.109627808255716	20.524942298101344	25.80637678469943
2	25.255222166807776	30.194040756677765	19.45139702385785	25.099340052656604
3	21.28997088808731	31.770259455572436	20.490379150767232	26.449390505573017
4	20.904209049049513	33.40725362159153	18.132430206798965	27.556107122559993
5	18.6490216773565	35.19804219487584	17.361370463586912	28.79156566418075
6	21.139887959730455	37.48848861619828	19.71769563553277	21.65392778853849
7	84.81146846982683	6.965981976130552	4.769018429812454	3.453531124230158
8	85.26960414757768	6.849302357948944	4.23526136930375	3.645832125169626
9	76.71998051473572	10.886231573087139	7.812431134668693	4.581356777508438
10	32.010577714888825	38.729050441318044	14.9166657001357	14.343706143657432
11	23.6316821118315	31.009870214221923	27.260348647049952	18.098099026896623
12	24.400886115589373	27.53267841195096	29.452208909869054	18.614226562590613
13	23.12993655690741	28.968092879759684	28.138577343740938	19.76339321959197
14	19.778239135225416	29.947227409271736	29.817093680047325	20.457439775455526
15	19.29667474686554	29.76490100789849	30.195432561268397	20.74299168396757
16	21.48203992159501	30.95280622600587	27.489300502209492	20.075853350189636
17	20.700309676521417	28.96577320544196	29.670722230598823	20.663194887437804
18	22.180725826094015	27.813590971827555	28.87089852584697	21.134784676231458
19	22.8745404145258	30.067850473793477	26.76277850589777	20.29483060578295
20	23.728876465744207	29.152043053155335	27.551931708788086	19.56714877231237
21	22.950625732147206	30.51253204050151	27.639615397998117	18.89722682935316
22	22.241733260650204	28.576067920064023	27.28354539022721	21.89865342905856
23	20.54837100871038	29.991069253876756	28.120947818926222	21.339611918486646
24	19.88610399099966	29.403031814333268	29.70806898711421	21.00279520755286
25	20.736032661014395	31.283591783713565	27.075470603927208	20.90490495134483
26	22.078660156114083	30.555909950242988	26.313225623122516	21.05220427052042
27	20.556953803685964	29.830083856226587	26.86345237128707	22.74950996880038
28	20.744383488558206	31.36802792887878	27.81057539521451	20.0770131873485
29	21.901900973103377	30.822904464213224	26.40276505178673	20.87242951089667
30	21.70148111205187	29.956274139110867	28.187754439276723	20.15449030956054
31	21.62701956645287	31.428571428571427	26.005868776023846	20.938540228951855
32	22.541435182500376	29.82428467043227	27.21395516069544	20.420324986371913
33	20.616337466219743	31.481460003015577	26.165694336515156	21.736508194249527
34	20.219905125320405	29.897818346304177	29.432491678168386	20.44978485020703
35	21.552789988285646	30.851436458321253	28.588594161379742	19.007179392013363
36	21.446780871965576	32.706248042774796	27.078718147972026	18.768252937287606
37	20.711676080678274	31.89273825954836	26.691796471775366	20.703789187998005
38	22.80147067351744	30.7317412635266	27.012839397348614	19.45394866560735
39	20.95872139551607	30.586529651236965	27.15828297707002	21.296465976176947
40	22.777114093181318	30.530625500179774	27.192382189540588	19.499878217098317
41	20.020413133995987	29.75005509226505	29.46496711861655	20.76456465512242
42	21.312471728969253	30.134193159280436	27.37262088402788	21.180714227722426
43	21.127593685846506	29.212818520279754	28.555190851204493	21.104396942669247
44	21.916978856168594	29.18011111239982	27.873902504088427	21.02900752734316
45	22.316194806249204	28.106333870724548	28.54614412136536	21.031327201660886
46	19.336805112562196	30.73893225391155	28.428072698593116	21.496189934933135
47	20.064950880896323	31.527621521938322	29.068302810285434	19.33912478687992
48	20.418005312054188	29.750750994560367	29.179415210104505	20.651828483280948
49	20.88472378478062	29.45452858418678	28.312784885002145	21.347962746030458
50	20.450944687365894	30.340180238694487	28.115844535427225	21.09303053851239
51	20.08536401489231	28.81128289588142	28.813834537630918	22.289518551595357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6039.0
1	4658.0
2	3277.0
3	2143.0
4	1009.0
5	910.5
6	812.0
7	901.5
8	991.0
9	1073.0
10	1155.0
11	1218.0
12	1281.0
13	1329.5
14	1378.0
15	1430.0
16	1482.0
17	1413.5
18	1345.0
19	1362.5
20	1380.0
21	1532.0
22	1684.0
23	2341.5
24	2999.0
25	3538.5
26	5370.5
27	6663.0
28	8292.0
29	9921.0
30	11330.0
31	12739.0
32	14185.5
33	15632.0
34	17398.5
35	19165.0
36	20058.0
37	20951.0
38	21698.0
39	22445.0
40	23071.0
41	23697.0
42	24807.5
43	25918.0
44	25874.5
45	25831.0
46	27029.0
47	28227.0
48	29203.5
49	30180.0
50	29588.0
51	28996.0
52	26891.0
53	24786.0
54	22617.5
55	20449.0
56	18485.0
57	16521.0
58	15615.5
59	14710.0
60	13894.5
61	13079.0
62	12238.5
63	11398.0
64	9964.0
65	8530.0
66	7283.0
67	6036.0
68	5510.0
69	4984.0
70	4258.5
71	3533.0
72	3077.0
73	2621.0
74	2387.5
75	1646.0
76	1138.0
77	1032.5
78	927.0
79	665.0
80	403.0
81	345.0
82	287.0
83	222.5
84	158.0
85	100.5
86	43.0
87	33.5
88	24.0
89	28.0
90	32.0
91	17.5
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.30260394607643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45422759776139	17.582799817677436
2	8.228542717695554	3.8349294400766167
3	3.0388929535737987	2.1244235679495795
4	1.6119199946505884	1.5024773291241722
5	1.0319484159784904	1.2023542615163847
6	0.8369581557037262	1.17019826530815
7	0.6589670543506394	1.07489537967319
8	0.5619719037073267	1.0476326960731548
9	0.5229738534499898	1.0967987322988677
>10	6.529673543075377	34.31929137197916
>50	1.0239488067548526	16.70736475558973
>100	0.49597520327188166	16.48833122927553
>500	0.0019999000131930714	0.3758522105795768
>1k	9.999500065965357E-4	0.2654033898636937
>5k	9.999500065965357E-4	1.2072475530147473
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5181	1.2018232640137325	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1139	0.26421090478896764	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	807	0.18719771744047134	No Hit
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	806	0.18696575000869878	TruSeq Adapter, Index 1 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	499	0.115751748454517	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22152889734281306	0.0
2	0.0	0.0	0.0	0.30294946589498833	0.0
3	0.0	0.0	0.0	0.3843700344471636	0.0
4	0.0	0.0	0.0	0.8035351836602141	0.0
5	0.0	0.0	0.0	0.8638467159210846	0.0
6	0.0	0.0	0.0	1.3229102633990188	0.0
7	0.0	0.0	0.0	1.6112457810923346	0.0
8	0.0	0.0	0.0	1.9724190723622403	0.0
9	0.0	0.0	0.0	2.3906563518482007	0.0
10	0.0	0.0	0.0	3.1252972082719586	0.0
11	0.0	0.0	0.0	3.6752919890047435	0.0
12	0.0	0.0	0.0	3.975689813150234	0.0
13	2.3196743177257914E-4	0.0	0.0	4.121597327735186	0.0
14	2.3196743177257914E-4	0.0	0.0	4.252426959254921	0.0
15	2.3196743177257914E-4	0.0	0.0	4.36655493568703	0.0
16	2.3196743177257914E-4	0.0	0.0	4.603393683526833	0.0
17	2.3196743177257914E-4	0.0	0.0	4.8683004906111185	0.0
18	2.3196743177257914E-4	0.0	0.0	5.270995952168316	0.0
19	2.3196743177257914E-4	0.0	0.0	5.414119857571997	0.0
20	2.3196743177257914E-4	0.0	0.0	5.6034052818984215	0.0
21	2.3196743177257914E-4	0.0	0.0	5.793850543383709	0.0
22	2.3196743177257914E-4	0.0	0.0	5.992646632412809	0.0
23	2.3196743177257914E-4	0.0	0.0	6.207216506802445	0.0
24	2.3196743177257914E-4	0.0	0.0	6.358923207181712	0.0
25	2.3196743177257914E-4	0.0	0.0	6.492072513019172	0.0
26	2.3196743177257914E-4	0.0	0.0	6.616175089017502	0.0
27	2.3196743177257914E-4	0.0	0.0	6.776696551804127	0.0
28	2.3196743177257914E-4	0.0	0.0	6.943713102680384	0.0
29	2.3196743177257914E-4	0.0	0.0	7.090548486992426	0.0
30	2.3196743177257914E-4	0.0	0.0	7.3445528247834	0.0
31	4.639348635451583E-4	0.0	0.0	7.4883726324824	0.0
32	4.639348635451583E-4	0.0	0.0	7.660028531994108	0.0
33	4.639348635451583E-4	0.0	0.0	7.827972952597455	0.0
34	4.639348635451583E-4	0.0	0.0	7.9729525974553175	0.0
35	4.639348635451583E-4	0.0	0.0	8.130922418492444	0.0
36	4.639348635451583E-4	0.0	0.0	8.28518076062121	0.0
37	4.639348635451583E-4	0.0	0.0	8.453357148656329	0.0
38	4.639348635451583E-4	0.0	0.0	8.613414676579408	0.0
39	4.639348635451583E-4	0.0	0.0	8.795509110520882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	35	1.2095734E-7	45.000004	23
CGAACTT	20	7.028374E-4	45.000004	39
CCGATTT	40	6.7975634E-9	45.000004	18
TTGTCGT	20	7.028374E-4	45.000004	5
CACGAAA	20	7.028374E-4	45.000004	1
AAGCGCG	20	7.028374E-4	45.000004	1
CGTTCTA	20	7.028374E-4	45.000004	42
CTGACGT	20	7.028374E-4	45.000004	30
ACGATAG	20	7.028374E-4	45.000004	1
TTACGTG	20	7.028374E-4	45.000004	1
ATCGGCG	20	7.028374E-4	45.000004	1
CGGTAAC	35	1.2095734E-7	45.000004	26
TAATGCC	20	7.028374E-4	45.000004	35
AATCGCA	35	1.2095734E-7	45.000004	25
CTTAACG	25	3.886735E-5	45.0	1
GCCCATC	25	3.886735E-5	45.0	31
CTACTGA	25	3.886735E-5	45.0	41
ACGCATG	25	3.886735E-5	45.0	1
CGACGAG	25	3.886735E-5	45.0	1
TAGCGTC	25	3.886735E-5	45.0	10
>>END_MODULE