##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703135
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.131552262367826	33.0	31.0	34.0	30.0	34.0
2	32.320848770150825	34.0	31.0	34.0	30.0	34.0
3	32.42328713547185	34.0	31.0	34.0	30.0	34.0
4	35.982432960953446	37.0	35.0	37.0	35.0	37.0
5	35.795768949063834	37.0	35.0	37.0	35.0	37.0
6	35.77016789094555	37.0	35.0	37.0	35.0	37.0
7	35.94422550434838	37.0	35.0	37.0	35.0	37.0
8	36.07485049101524	37.0	35.0	37.0	35.0	37.0
9	37.68276504511936	39.0	38.0	39.0	35.0	39.0
10	37.13469390657555	39.0	37.0	39.0	34.0	39.0
11	37.05652257390117	39.0	37.0	39.0	33.0	39.0
12	37.151231271377476	39.0	37.0	39.0	34.0	39.0
13	37.143579824642494	39.0	37.0	39.0	33.0	39.0
14	38.39134163425231	40.0	38.0	41.0	34.0	41.0
15	38.40153313375099	40.0	38.0	41.0	34.0	41.0
16	38.35742922767321	40.0	38.0	41.0	34.0	41.0
17	38.322653544482925	40.0	38.0	41.0	34.0	41.0
18	38.243786755032815	40.0	37.0	41.0	33.0	41.0
19	38.221148143670845	40.0	37.0	41.0	34.0	41.0
20	38.16765912662576	40.0	37.0	41.0	33.0	41.0
21	38.12255967915123	40.0	37.0	41.0	33.0	41.0
22	38.05156619994738	40.0	37.0	41.0	33.0	41.0
23	37.966930959204134	40.0	37.0	41.0	33.0	41.0
24	37.89187851550556	40.0	37.0	41.0	33.0	41.0
25	37.82682984064227	40.0	37.0	41.0	33.0	41.0
26	37.738936335127676	40.0	37.0	41.0	33.0	41.0
27	37.65740291693629	40.0	37.0	41.0	33.0	41.0
28	37.61497720921302	40.0	36.0	41.0	33.0	41.0
29	37.47936029354249	40.0	36.0	41.0	32.0	41.0
30	37.41680616097904	39.0	36.0	41.0	32.0	41.0
31	37.36871297830431	39.0	36.0	41.0	32.0	41.0
32	37.289836233440234	39.0	36.0	41.0	32.0	41.0
33	37.17994979626956	39.0	35.0	41.0	31.0	41.0
34	37.11542591394256	39.0	35.0	41.0	31.0	41.0
35	37.08720515974884	39.0	35.0	41.0	31.0	41.0
36	36.98004935040924	39.0	35.0	41.0	31.0	41.0
37	36.932089854722065	39.0	35.0	41.0	31.0	41.0
38	36.86619639187354	39.0	35.0	41.0	31.0	41.0
39	36.74993137875372	39.0	35.0	41.0	31.0	41.0
40	36.69574121612493	39.0	35.0	41.0	31.0	41.0
41	36.61353651859174	39.0	35.0	40.0	30.0	41.0
42	36.53045147802342	39.0	35.0	40.0	30.0	41.0
43	36.46693024810314	39.0	35.0	40.0	30.0	41.0
44	36.37212910749714	39.0	35.0	40.0	30.0	41.0
45	36.238597139951786	38.0	35.0	40.0	30.0	41.0
46	36.12806786747922	38.0	35.0	40.0	29.0	41.0
47	35.99880961692989	38.0	35.0	40.0	29.0	41.0
48	35.902013126924416	38.0	35.0	40.0	29.0	41.0
49	35.752114458816585	38.0	34.0	40.0	28.0	41.0
50	35.618303739680144	38.0	34.0	40.0	28.0	41.0
51	34.62816813271989	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	6.0
12	6.0
13	15.0
14	12.0
15	14.0
16	44.0
17	88.0
18	156.0
19	315.0
20	561.0
21	860.0
22	1261.0
23	1842.0
24	2490.0
25	3358.0
26	4399.0
27	5385.0
28	7178.0
29	9082.0
30	11784.0
31	15173.0
32	19962.0
33	26428.0
34	37988.0
35	52114.0
36	67599.0
37	92532.0
38	143578.0
39	198810.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.98060827579341	32.73126782196876	19.383475435016035	26.904648467221797
2	22.24636805165438	32.3285002168858	19.765478890966882	25.659652840492935
3	20.000995541396744	31.890319782118652	21.615194806118314	26.49348987036629
4	19.706173067760815	33.00034843948886	20.39722101730109	26.896257475449236
5	17.638291366522786	35.601271448583844	17.818768799732627	28.941668385160746
6	19.80103394085062	38.80407034211069	19.134732306029427	22.260163411009266
7	87.14201398024561	5.61456903724036	3.753759946525205	3.489657035988822
8	88.51216338256522	5.374928001023985	3.2084877015082456	2.9044209149025435
9	79.91324567828369	10.933604499847114	5.819792785169278	3.333357036699922
10	30.443087031651107	46.303768124186675	11.973518598846594	11.279626245315622
11	18.674365520134824	28.581282399539205	35.79767754414159	16.946674536184375
12	23.397356126490646	25.77328677992135	32.62175826832685	18.20759882526115
13	22.58101218116009	27.859657107098922	30.1886550946831	19.37067561705789
14	18.682756511907385	30.430856094491098	29.43815910173722	21.448228291864293
15	18.21670091803139	32.72600567458596	29.935076478912297	19.12221692847035
16	19.95477397654789	32.103081200622924	28.698898504554606	19.243246318274583
17	19.029347138174035	32.31186045354021	30.115553912122138	18.54323849616361
18	19.003178621459607	31.880506588350745	29.315423069538564	19.800891720651084
19	18.669387813151104	33.8622028486706	28.70117402774716	18.76723531043114
20	20.69431901412958	32.863105946937644	28.17197266527765	18.27060237365513
21	20.338199634494085	33.91951758908318	27.15282271541027	18.589460061012467
22	19.300561058687165	31.901270737482847	28.072560745802726	20.725607458027266
23	19.212953415773644	33.70149402319612	27.59299423297091	19.492558328059335
24	17.609705106416264	34.11976363002837	29.166091860026878	19.104439403528485
25	18.334459243246318	35.751171538893665	25.842405796895335	20.071963420964682
26	19.7728743413427	34.73344379102164	25.690087963193413	19.80359390444225
27	18.42889345573752	33.694667453618436	27.37852617207222	20.497912918571824
28	19.320329666422523	34.381875457771265	27.989930809872927	18.307864065933284
29	19.03475150575636	33.818114586814765	26.463481408264418	20.683652499164456
30	19.504362604620734	34.44317236377083	26.88075547369993	19.17170955790851
31	19.455438855980713	34.33167172733543	25.729198518065523	20.48369089861833
32	20.790317648815662	33.67959211246773	26.073798061538678	19.456292177177925
33	18.152417387841595	34.51584688573318	27.371272941895935	19.960462784529287
34	17.699019391724207	33.8829669978027	28.008134995413396	20.409878615059696
35	19.07869754741266	33.49755025706301	27.338562296002898	20.08518989952143
36	18.11088908957739	35.296493560980466	27.57493226762997	19.017685081812168
37	17.199257610558426	36.03916744295192	28.630775028977368	18.130799917512284
38	18.044330036195042	34.874384008760764	29.159265290449206	17.922020664594992
39	17.68522403236932	36.401686731566485	26.587781862657955	19.325307373406243
40	19.742439218642225	34.45483442013269	27.25223463488519	18.550491726339892
41	17.172235772646786	34.520824592716906	28.80855027839604	19.498389356240267
42	17.93354050075732	34.863859713995176	26.559764483349568	20.64283530189793
43	17.70655706229956	34.90723687485334	27.470542641171324	19.91566342167578
44	19.429270339266285	33.618295206468176	27.005909249290678	19.94652520497486
45	18.76083540145207	34.609285556827636	26.88815092407575	19.74172811764455
46	17.415290093652004	35.94629765265561	27.46983154017365	19.16858071351874
47	17.709828126888862	34.93909419954917	27.32576247804476	20.02531519551722
48	17.860297097996828	34.01651176516601	28.83585655670675	19.287334580130416
49	17.64995342288465	35.26861840187161	27.40796575337595	19.673462421867775
50	17.838395187268446	33.51006563462208	27.829790865196586	20.821748312912884
51	17.45269400612969	32.84433288059903	28.69505855916716	21.007914554104122
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6843.0
1	5264.0
2	3685.0
3	2492.5
4	1300.0
5	1362.0
6	1424.0
7	1804.5
8	2185.0
9	2781.0
10	3377.0
11	4360.5
12	5344.0
13	5787.5
14	6231.0
15	6422.5
16	6614.0
17	6595.5
18	6577.0
19	6880.5
20	7184.0
21	7191.5
22	7199.0
23	7408.0
24	7617.0
25	8666.0
26	10685.5
27	11656.0
28	14527.5
29	17399.0
30	19350.0
31	21301.0
32	25607.0
33	29913.0
34	32133.5
35	34354.0
36	34917.5
37	35481.0
38	36714.0
39	37947.0
40	39808.5
41	41670.0
42	42921.0
43	44172.0
44	47719.5
45	51267.0
46	53979.0
47	56691.0
48	54889.0
49	53087.0
50	50590.5
51	48094.0
52	42197.0
53	36300.0
54	31341.0
55	26382.0
56	23510.5
57	20639.0
58	19277.0
59	17915.0
60	16412.0
61	14909.0
62	12943.0
63	10977.0
64	8779.0
65	6581.0
66	5449.0
67	4317.0
68	3377.5
69	2438.0
70	1999.0
71	1560.0
72	1284.0
73	1008.0
74	875.0
75	631.5
76	521.0
77	386.5
78	252.0
79	193.5
80	135.0
81	97.5
82	60.0
83	45.0
84	30.0
85	24.5
86	19.0
87	16.0
88	13.0
89	7.0
90	1.0
91	2.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	703135.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.372654430915922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.09336307300538	21.592941489480314
2	9.325232671073751	5.664641388128201
3	4.336395918468192	3.9512356412180596
4	2.8200829717629676	3.426136222714682
5	1.953660736278115	2.9668931209111977
6	1.4901452520320384	2.7155800075103533
7	1.1387996943442416	2.4211858707645226
8	0.8897558175473436	2.1619396779410036
9	0.7108130224619589	1.9430350466608757
>10	5.511641150088898	33.6499551422734
>50	0.6043424103529084	12.564564302137454
>100	0.12105689746866671	4.904905577314913
>500	0.0023551925577561614	0.462106084900454
>1k	0.001884154046204929	0.7287827852888275
>5k	4.7103851155123227E-4	0.8460976427558159
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5914	0.8410902600496348	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	1569	0.22314349307032078	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1353	0.19242392997077376	No Hit
AACTTTGGGTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1104	0.1570111002865737	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1068	0.15189117310331587	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	851	0.12102938980423389	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	802	0.11406060002702184	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.844403990698799E-4	0.0	0.0	0.27107170031359556	0.0
2	2.844403990698799E-4	0.0	0.0	0.402625384883415	0.0
3	2.844403990698799E-4	0.0	0.0	0.5277791604741622	0.0
4	2.844403990698799E-4	0.0	0.0	1.0630959915236762	0.0
5	2.844403990698799E-4	0.0	0.0	1.162650131198134	0.0
6	2.844403990698799E-4	0.0	0.0	1.804632111898853	0.0
7	2.844403990698799E-4	0.0	0.0	2.301549489073933	0.0
8	2.844403990698799E-4	0.0	0.0	2.8364396595248422	0.0
9	2.844403990698799E-4	0.0	0.0	3.4634885192743927	0.0
10	2.844403990698799E-4	0.0	0.0	4.421341563142213	0.0
11	2.844403990698799E-4	0.0	0.0	5.230432278296487	0.0
12	2.844403990698799E-4	0.0	0.0	5.628933277393388	0.0
13	2.844403990698799E-4	0.0	0.0	5.796895333044152	0.0
14	2.844403990698799E-4	0.0	0.0	5.9713995178735235	0.0
15	2.844403990698799E-4	0.0	0.0	6.124855113171724	0.0
16	2.844403990698799E-4	0.0	0.0	6.424086412993238	0.0
17	2.844403990698799E-4	0.0	0.0	6.826000696878978	0.0
18	2.844403990698799E-4	0.0	0.0	7.271718802221479	9.955413967445796E-4
19	2.844403990698799E-4	0.0	0.0	7.518186408015531	9.955413967445796E-4
20	2.844403990698799E-4	0.0	0.0	7.766645096603071	9.955413967445796E-4
21	2.844403990698799E-4	0.0	0.0	8.05307657846644	9.955413967445796E-4
22	2.844403990698799E-4	0.0	0.0	8.401516067327043	9.955413967445796E-4
23	2.844403990698799E-4	0.0	0.0	8.740995683616944	9.955413967445796E-4
24	2.844403990698799E-4	0.0	0.0	9.003534171958444	9.955413967445796E-4
25	2.844403990698799E-4	0.0	0.0	9.228953188221324	9.955413967445796E-4
26	2.844403990698799E-4	0.0	0.0	9.44740341470699	9.955413967445796E-4
27	2.844403990698799E-4	0.0	0.0	9.67396019256615	9.955413967445796E-4
28	2.844403990698799E-4	0.0	0.0	9.907201319803452	9.955413967445796E-4
29	2.844403990698799E-4	0.0	0.0	10.15992661437704	9.955413967445796E-4
30	2.844403990698799E-4	0.0	0.0	10.494428523683219	9.955413967445796E-4
31	4.2666059860481983E-4	0.0	0.0	10.744024973867038	9.955413967445796E-4
32	4.2666059860481983E-4	0.0	0.0	10.993052543252718	9.955413967445796E-4
33	4.2666059860481983E-4	0.0	0.0	11.24904890241561	9.955413967445796E-4
34	4.2666059860481983E-4	0.0	0.0	11.499072013198035	9.955413967445796E-4
35	4.2666059860481983E-4	0.0	0.0	11.789627880847917	9.955413967445796E-4
36	4.2666059860481983E-4	0.0	0.0	12.071366096126633	9.955413967445796E-4
37	4.2666059860481983E-4	0.0	0.0	12.343717778236043	9.955413967445796E-4
38	4.2666059860481983E-4	0.0	0.0	12.602842981788703	9.955413967445796E-4
39	4.2666059860481983E-4	0.0	0.0	12.872065819508345	9.955413967445796E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2110286E-7	45.000004	1
AACCGAG	35	1.2110286E-7	45.000004	1
ATTAGCG	35	1.2110286E-7	45.000004	1
TTAGCGG	70	0.0	45.000004	2
CCGTAGG	35	1.2110286E-7	45.000004	2
TATCCCG	35	1.2110286E-7	45.000004	1
AAACTCG	20	7.0314156E-4	45.0	1
CGAACAG	45	3.8380676E-10	45.0	1
CGACGAA	20	7.0314156E-4	45.0	17
ACTTGCG	20	7.0314156E-4	45.0	1
CGTGACG	20	7.0314156E-4	45.0	1
TAGCGGC	20	7.0314156E-4	45.0	27
ATTACCG	20	7.0314156E-4	45.0	1
TGGCGAT	20	7.0314156E-4	45.0	18
ATAGACG	45	3.8380676E-10	45.0	1
AAGCGCG	25	3.8892547E-5	45.0	1
CGCGAAG	40	6.8084773E-9	45.0	1
TATGCGG	50	2.1827873E-11	45.0	2
CATTACG	25	3.8892547E-5	45.0	1
AGCGGCC	25	3.8892547E-5	45.0	4
>>END_MODULE