##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	685919
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20594851578685	33.0	31.0	34.0	30.0	34.0
2	32.37764663174515	34.0	31.0	34.0	30.0	34.0
3	32.475741304731315	34.0	31.0	34.0	30.0	34.0
4	36.00753150153298	37.0	35.0	37.0	35.0	37.0
5	35.84523391245905	37.0	35.0	37.0	35.0	37.0
6	35.835878580415475	37.0	35.0	37.0	35.0	37.0
7	35.974320583042605	37.0	35.0	37.0	35.0	37.0
8	36.10069556317874	37.0	35.0	37.0	35.0	37.0
9	37.7213256958912	39.0	38.0	39.0	35.0	39.0
10	37.19900892087841	39.0	37.0	39.0	34.0	39.0
11	37.1441802895094	39.0	37.0	39.0	34.0	39.0
12	37.24997558020699	39.0	37.0	39.0	34.0	39.0
13	37.24561063332551	39.0	37.0	39.0	34.0	39.0
14	38.51064484290419	40.0	38.0	41.0	34.0	41.0
15	38.505062551117554	40.0	38.0	41.0	34.0	41.0
16	38.47735082422268	40.0	38.0	41.0	34.0	41.0
17	38.42899234457713	40.0	38.0	41.0	34.0	41.0
18	38.35140738192119	40.0	38.0	41.0	34.0	41.0
19	38.34289471497363	40.0	38.0	41.0	34.0	41.0
20	38.28293865602207	40.0	38.0	41.0	34.0	41.0
21	38.231606064272896	40.0	37.0	41.0	34.0	41.0
22	38.169925311880846	40.0	37.0	41.0	33.0	41.0
23	38.086100545399674	40.0	37.0	41.0	33.0	41.0
24	38.032188931929284	40.0	37.0	41.0	33.0	41.0
25	37.974295798775074	40.0	37.0	41.0	33.0	41.0
26	37.90146941548492	40.0	37.0	41.0	33.0	41.0
27	37.84644542577185	40.0	37.0	41.0	33.0	41.0
28	37.761232740308984	40.0	37.0	41.0	33.0	41.0
29	37.6658687104454	40.0	36.0	41.0	33.0	41.0
30	37.59355259148675	40.0	36.0	41.0	33.0	41.0
31	37.52158491017161	40.0	36.0	41.0	33.0	41.0
32	37.44578733057402	40.0	36.0	41.0	32.0	41.0
33	37.356075571605395	39.0	36.0	41.0	32.0	41.0
34	37.287730767043925	39.0	36.0	41.0	32.0	41.0
35	37.22686497968419	39.0	35.0	41.0	32.0	41.0
36	37.150904115500516	39.0	35.0	41.0	31.0	41.0
37	37.08874079884068	39.0	35.0	41.0	31.0	41.0
38	37.03096429753367	39.0	35.0	41.0	31.0	41.0
39	36.92362800855495	39.0	35.0	41.0	31.0	41.0
40	36.843701661566456	39.0	35.0	41.0	31.0	41.0
41	36.762724170055066	39.0	35.0	41.0	31.0	41.0
42	36.707408600723994	39.0	35.0	41.0	31.0	41.0
43	36.64036132546263	39.0	35.0	41.0	31.0	41.0
44	36.56096711127699	39.0	35.0	41.0	31.0	41.0
45	36.419914013170654	39.0	35.0	40.0	30.0	41.0
46	36.26838154359334	38.0	35.0	40.0	30.0	41.0
47	36.17934916513466	38.0	35.0	40.0	30.0	41.0
48	36.07937963520474	38.0	35.0	40.0	29.0	41.0
49	35.97816943400022	38.0	35.0	40.0	29.0	41.0
50	35.883506653117934	38.0	35.0	40.0	29.0	41.0
51	34.93373561601297	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	7.0
12	7.0
13	8.0
14	17.0
15	25.0
16	46.0
17	86.0
18	143.0
19	288.0
20	524.0
21	734.0
22	1157.0
23	1741.0
24	2284.0
25	3027.0
26	3907.0
27	4979.0
28	6260.0
29	8162.0
30	10683.0
31	13906.0
32	18107.0
33	24300.0
34	35129.0
35	49236.0
36	64164.0
37	88928.0
38	140780.0
39	207168.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.965449273164907	32.60195445817946	19.30650703654513	27.126089232110495
2	22.286450732520898	32.34142806949509	19.583070304219593	25.789050893764426
3	20.06242719621413	31.791217330326177	21.695418846831767	26.450936626627925
4	19.749562266098476	32.99835694885256	20.294087202716355	26.957993582332605
5	17.656457978274403	35.482761084034706	17.85691896565046	29.00386197204043
6	19.814001361676816	38.91829793313788	19.181710960040473	22.085989745144836
7	87.04438862314646	5.605472366270653	3.819255626393204	3.5308833841896785
8	88.31494680858819	5.408801913928612	3.299660747114455	2.976590530368746
9	79.82137832601227	10.929716190978818	5.863666118011019	3.385239364997908
10	30.348044011027543	46.324274440568054	12.126942102493151	11.200739445911251
11	18.64855762852465	28.673939634271683	35.544138593624034	17.133364143579634
12	23.295607790424235	25.80727461988952	32.70998470664904	18.18713288303721
13	22.411101019216556	28.023133926892243	30.282001227550193	19.283763826341012
14	18.796680074469435	30.360144565174608	29.550865335411324	21.292310024944637
15	18.29530892131578	32.642629814890675	29.973947361131565	19.088113902661977
16	20.04653610703305	31.929134489640905	28.727590283983968	19.296739119342078
17	18.965067303865325	32.324953821078	30.208814743431805	18.50116413162487
18	19.07222281348089	31.779845725224114	29.449104048728785	19.698827412566207
19	18.678007169942806	33.792619828288764	28.819729443272458	18.709643558495976
20	20.6614775213983	32.790314891408464	28.25887604804649	18.28933153914675
21	20.309103553043435	33.774979261399665	27.382679295951856	18.533237889605044
22	19.14161876256526	32.127117050263955	28.079700372784544	20.651563814386247
23	19.239152144786775	33.60790414028478	27.57410131516987	19.57884239975857
24	17.599745742573102	34.18129545908482	29.013921468861483	19.205037329480596
25	18.227662449939423	35.80976762562344	25.87623319954688	20.08633672489026
26	19.806274501799777	34.67246132560842	25.77068137783033	19.75058279476148
27	18.310616851260864	33.7993261595028	27.34214972904964	20.547907260186697
28	19.24206794096679	34.33787371395165	28.054624525636406	18.365433819445155
29	19.098319189292027	33.800200898356806	26.511002027936243	20.590477884414923
30	19.38975301748457	34.476957191738386	26.84908859500903	19.284201195768013
31	19.399520934687622	34.31352681584852	25.79371616765245	20.493236081811407
32	20.63654746405917	33.7990345798848	26.112850059555136	19.451567896500897
33	18.25507093403157	34.45231871401725	27.27319114939227	20.019419202558904
34	17.64654427126235	33.74115602571149	28.138016296384848	20.47428340664131
35	19.022071119184627	33.412837375841754	27.375389805501815	20.189701699471804
36	18.14543699766299	35.31670649158283	27.592033461676962	18.945823049077223
37	17.143132060782687	35.94199898238713	28.71024129671288	18.204627660117303
38	17.94497601028693	34.85630227475839	29.178226583605355	18.02049513134933
39	17.636630564250297	36.40531899539158	26.672682926118096	19.285367514240022
40	19.766328094133563	34.5170493892136	27.17361670984475	18.543005806808093
41	17.21981750031709	34.51384201341558	28.742315054693044	19.524025431574284
42	17.96990606762606	34.76285100718889	26.63302809806989	20.634214827115155
43	17.681096455995533	34.998009969107144	27.50135220047848	19.819541374418844
44	19.447923151275877	33.62437838870188	26.99662788171781	19.931070578304436
45	18.804115354728474	34.440655529297196	26.947350926275554	19.807878189698783
46	17.322890895280636	36.05906819901475	27.449888397901212	19.1681525078034
47	17.775568252228034	35.020461599693256	27.225955251276023	19.978014896802684
48	17.837966290480363	34.014366127778935	28.880669583434777	19.266997998305925
49	17.65733271712841	35.28973537691768	27.380492448816845	19.672439457137067
50	17.78985565351011	33.51445287271529	27.81990293314517	20.875788540629433
51	17.482530736136482	32.82763708251266	28.741731895457047	20.948100285893815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6543.0
1	5114.5
2	3686.0
3	2484.5
4	1283.0
5	1420.0
6	1557.0
7	1860.0
8	2163.0
9	2745.0
10	3327.0
11	4331.5
12	5336.0
13	5718.0
14	6100.0
15	6307.5
16	6515.0
17	6527.0
18	6539.0
19	6711.5
20	6884.0
21	6984.5
22	7085.0
23	7324.0
24	7563.0
25	8631.5
26	10504.0
27	11308.0
28	14175.0
29	17042.0
30	18904.0
31	20766.0
32	24911.5
33	29057.0
34	31271.0
35	33485.0
36	34004.0
37	34523.0
38	35428.5
39	36334.0
40	38372.5
41	40411.0
42	41686.5
43	42962.0
44	46406.5
45	49851.0
46	52611.5
47	55372.0
48	53584.5
49	51797.0
50	49263.5
51	46730.0
52	41000.0
53	35270.0
54	30832.0
55	26394.0
56	23284.0
57	20174.0
58	18877.5
59	17581.0
60	16042.0
61	14503.0
62	12688.5
63	10874.0
64	8720.0
65	6566.0
66	5382.5
67	4199.0
68	3278.5
69	2358.0
70	1953.0
71	1548.0
72	1233.5
73	919.0
74	809.0
75	581.0
76	463.0
77	341.0
78	219.0
79	168.5
80	118.0
81	88.0
82	58.0
83	39.5
84	21.0
85	18.0
86	15.0
87	14.5
88	14.0
89	8.0
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	685919.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.21538324894687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.38026049650917	21.265665440627394
2	9.527665504967766	5.757641294007439
3	4.492390487667864	4.072179008664234
4	2.904778311031832	3.5107595968422154
5	2.080060643179152	3.1424914757354503
6	1.4844908339973795	2.691267568726775
7	1.1757542513793457	2.486810571840388
8	0.9154038229477276	2.212742187033335
9	0.6860173233546651	1.8655448706520121
>10	5.635850738118375	34.00379325554053
>50	0.6005195135853274	12.416021608535026
>100	0.11244594604416275	4.596568455437731
>500	0.0019387232076579785	0.39643517408066836
>1k	0.0019387232076579785	0.7458604882130934
>5k	4.8468080191449463E-4	0.8362190040637667
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5710	0.8324598093944038	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	1663	0.24244845236828255	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1310	0.19098464979101032	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1106	0.16124352875485298	No Hit
AACTTTGGGTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1014	0.14783086632678202	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	859	0.12523344593166247	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	813	0.118527114717627	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4578980900077122E-4	0.0	0.0	0.27889590461847535	0.0
2	2.9157961800154245E-4	0.0	0.0	0.41477200660719415	0.0
3	2.9157961800154245E-4	0.0	0.0	0.546128624516889	0.0
4	2.9157961800154245E-4	0.0	0.0	1.0931319878877828	0.0
5	2.9157961800154245E-4	0.0	0.0	1.2080143573803903	0.0
6	2.9157961800154245E-4	0.0	0.0	1.8540090010628076	0.0
7	2.9157961800154245E-4	0.0	0.0	2.365731230655515	0.0
8	2.9157961800154245E-4	0.0	0.0	2.8974266640813275	0.0
9	2.9157961800154245E-4	0.0	0.0	3.546482893752761	0.0
10	2.9157961800154245E-4	0.0	0.0	4.514381435708881	0.0
11	2.9157961800154245E-4	0.0	0.0	5.344800187777274	0.0
12	2.9157961800154245E-4	0.0	0.0	5.75796850648546	0.0
13	2.9157961800154245E-4	0.0	0.0	5.933645226331389	0.0
14	2.9157961800154245E-4	0.0	0.0	6.116174067200355	0.0
15	2.9157961800154245E-4	0.0	0.0	6.278146545000212	0.0
16	2.9157961800154245E-4	0.0	0.0	6.591011475115867	0.0
17	2.9157961800154245E-4	0.0	0.0	7.006220851150063	0.0
18	4.3736942700231367E-4	0.0	0.0	7.47260245014353	5.831592360030849E-4
19	4.3736942700231367E-4	0.0	0.0	7.718695647736832	5.831592360030849E-4
20	4.3736942700231367E-4	0.0	0.0	7.967413061892148	5.831592360030849E-4
21	4.3736942700231367E-4	0.0	0.0	8.260013208556696	5.831592360030849E-4
22	4.3736942700231367E-4	0.0	0.0	8.599703463528492	5.831592360030849E-4
23	4.3736942700231367E-4	0.0	0.0	8.951494272647354	5.831592360030849E-4
24	4.3736942700231367E-4	0.0	0.0	9.214790667702747	5.831592360030849E-4
25	4.3736942700231367E-4	0.0	0.0	9.439161183754933	5.831592360030849E-4
26	4.3736942700231367E-4	0.0	0.0	9.646911661581033	5.831592360030849E-4
27	4.3736942700231367E-4	0.0	0.0	9.880466935600268	5.831592360030849E-4
28	4.3736942700231367E-4	0.0	0.0	10.110669044012486	5.831592360030849E-4
29	4.3736942700231367E-4	0.0	0.0	10.372799120595872	5.831592360030849E-4
30	4.3736942700231367E-4	0.0	0.0	10.704616725881628	5.831592360030849E-4
31	4.3736942700231367E-4	0.0	0.0	10.961060999913984	5.831592360030849E-4
32	4.3736942700231367E-4	0.0	0.0	11.211382101968308	5.831592360030849E-4
33	4.3736942700231367E-4	0.0	0.0	11.47088796198968	5.831592360030849E-4
34	4.3736942700231367E-4	0.0	0.0	11.730831191438057	5.831592360030849E-4
35	4.3736942700231367E-4	0.0	0.0	12.018620274405578	5.831592360030849E-4
36	4.3736942700231367E-4	0.0	0.0	12.305243038901095	5.831592360030849E-4
37	4.3736942700231367E-4	0.0	0.0	12.586763160081585	5.831592360030849E-4
38	4.3736942700231367E-4	0.0	0.0	12.862889058329044	5.831592360030849E-4
39	4.3736942700231367E-4	0.0	0.0	13.146887606262547	5.831592360030849E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	40	6.8084773E-9	45.000004	1
TTGGACG	20	7.031294E-4	45.000004	42
CTCAACG	50	2.1827873E-11	45.000004	1
CGAAACG	25	3.889154E-5	45.000004	1
GCGAATG	20	7.031294E-4	45.000004	1
CGGCACG	20	7.031294E-4	45.000004	1
CCGGATG	25	3.889154E-5	45.000004	5
CCGGAAC	20	7.031294E-4	45.000004	38
ACCGACA	20	7.031294E-4	45.000004	11
GCGCCGA	20	7.031294E-4	45.000004	28
TCGATTG	20	7.031294E-4	45.000004	1
TAACGCG	40	6.8084773E-9	45.000004	1
GCACCGT	25	3.889154E-5	45.000004	45
TAACCCG	20	7.031294E-4	45.000004	1
CGCTATC	25	3.889154E-5	45.000004	40
CGCTACG	25	3.889154E-5	45.000004	1
TCTCGCG	25	3.889154E-5	45.000004	1
GATATAC	25	3.889154E-5	45.000004	9
CGCAGCG	20	7.031294E-4	45.000004	1
TTACGTA	20	7.031294E-4	45.000004	21
>>END_MODULE