##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	712722
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30453949786873	34.0	31.0	34.0	30.0	34.0
2	32.48629900578346	34.0	31.0	34.0	31.0	34.0
3	32.57482159944551	34.0	31.0	34.0	31.0	34.0
4	36.10288724074744	37.0	35.0	37.0	35.0	37.0
5	35.929673841974854	37.0	35.0	37.0	35.0	37.0
6	35.90490962815797	37.0	35.0	37.0	35.0	37.0
7	35.91715844326399	37.0	35.0	37.0	35.0	37.0
8	36.021213039586264	37.0	35.0	37.0	35.0	37.0
9	37.69594456183477	39.0	38.0	39.0	35.0	39.0
10	37.3206649998176	39.0	37.0	39.0	34.0	39.0
11	37.24385244176551	39.0	37.0	39.0	34.0	39.0
12	37.155151658009714	39.0	37.0	39.0	34.0	39.0
13	37.112936039577846	39.0	37.0	39.0	33.0	39.0
14	38.36024424670489	40.0	38.0	41.0	34.0	41.0
15	38.36985107797992	40.0	38.0	41.0	34.0	41.0
16	38.370692920942524	40.0	38.0	41.0	34.0	41.0
17	38.32534704976134	40.0	38.0	41.0	34.0	41.0
18	38.241357780452965	40.0	37.0	41.0	34.0	41.0
19	38.17311237761708	40.0	37.0	41.0	34.0	41.0
20	38.082308389526354	40.0	37.0	41.0	33.0	41.0
21	38.03622871189609	40.0	37.0	41.0	33.0	41.0
22	37.98793919648895	40.0	37.0	41.0	33.0	41.0
23	37.91824582375737	40.0	37.0	41.0	33.0	41.0
24	37.82789222165164	40.0	36.0	41.0	33.0	41.0
25	37.756168604308556	40.0	36.0	41.0	33.0	41.0
26	37.6496726633947	40.0	36.0	41.0	33.0	41.0
27	37.57174326034555	40.0	36.0	41.0	33.0	41.0
28	37.49482968113795	40.0	36.0	41.0	33.0	41.0
29	37.38660796215074	39.0	36.0	41.0	32.0	41.0
30	37.345180869960515	39.0	36.0	41.0	32.0	41.0
31	37.298539963688505	39.0	35.0	41.0	32.0	41.0
32	37.219478281854634	39.0	35.0	41.0	32.0	41.0
33	37.128831437783596	39.0	35.0	41.0	32.0	41.0
34	37.00777161361653	39.0	35.0	41.0	31.0	41.0
35	36.953325420009485	39.0	35.0	41.0	31.0	41.0
36	36.838259517736226	39.0	35.0	41.0	31.0	41.0
37	36.72440727240074	39.0	35.0	41.0	31.0	41.0
38	36.66163384882184	39.0	35.0	41.0	31.0	41.0
39	36.583903681940505	39.0	35.0	41.0	30.0	41.0
40	36.49937563313606	39.0	35.0	41.0	30.0	41.0
41	36.43050726650784	39.0	35.0	40.0	30.0	41.0
42	36.32572728216612	39.0	35.0	40.0	30.0	41.0
43	36.23130617547936	38.0	35.0	40.0	30.0	41.0
44	36.11991351466631	38.0	35.0	40.0	30.0	41.0
45	35.974037563032994	38.0	35.0	40.0	29.0	41.0
46	35.86908219474073	38.0	35.0	40.0	29.0	41.0
47	35.70802360527667	38.0	35.0	40.0	29.0	41.0
48	35.60002778081777	38.0	34.0	40.0	28.0	41.0
49	35.45367197869576	38.0	34.0	40.0	28.0	41.0
50	35.31768066651514	38.0	34.0	40.0	27.0	41.0
51	34.2658792628823	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	6.0
12	1.0
13	16.0
14	22.0
15	32.0
16	60.0
17	117.0
18	217.0
19	403.0
20	695.0
21	1052.0
22	1536.0
23	2154.0
24	2954.0
25	3810.0
26	4837.0
27	5975.0
28	7165.0
29	9040.0
30	11625.0
31	15310.0
32	20150.0
33	27493.0
34	43532.0
35	56965.0
36	63071.0
37	89865.0
38	145216.0
39	199317.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.844671554968134	31.45742659830902	18.517879341454314	27.180022505268536
2	25.497178423003643	30.244190582022163	19.27623954360887	24.98239145136533
3	22.413647958109895	31.792901018910598	20.22457564099326	25.568875381986246
4	22.10904111280415	32.341502016213894	19.106888800963066	26.442568070018886
5	19.260525141640077	35.058550178049785	16.72096553775525	28.95995914255488
6	21.183013853929022	38.34342141816865	18.59420643673129	21.87935829117103
7	83.4099410429312	7.499698339605064	4.967013786581584	4.123346830882167
8	84.48020406273413	6.957411164521369	4.597164111673275	3.965220661071217
9	75.98558764848006	11.146842667968718	7.712965223467215	5.154604460084016
10	36.13007596229666	33.8676230002722	14.163306310174233	15.83899472725691
11	25.965243110216885	31.10750054018257	24.574658843139403	18.352597506461144
12	27.33169454569945	26.11621361484562	27.848024896102547	18.704066943352387
13	25.179523011777384	26.905020470814705	26.6556946467206	21.259761870687306
14	21.222580473171867	28.75342700239364	28.11979986586636	21.904192658568135
15	20.378773210312016	30.660622234195102	28.916884844301144	20.04371971119174
16	22.440867547234404	27.999276015052153	28.048243214044184	21.511613223669258
17	21.561001344142596	29.210267116772037	27.44127443799967	21.787457101085696
18	22.541046859785443	28.603719262208827	27.804108754886197	21.051125123119533
19	22.859824728295184	30.21921590746462	26.653449732153632	20.267509632086565
20	23.9709171317849	29.287015133530325	26.784496620000503	19.957571114684267
21	23.269381329606777	29.99402291496544	26.034835461792955	20.701760293634823
22	22.2588891601494	29.224438139976034	26.24459466664422	22.272078033230347
23	22.519158942757482	29.479516557648004	26.048164642034344	21.95315985756017
24	21.455069438013698	28.85641245815339	27.99169942838863	21.69681867544428
25	21.67605321569981	31.200945109032695	25.711287149828404	21.41171452543909
26	22.559286790642076	29.813868520966096	25.928903555664064	21.69794113272777
27	21.653323455709238	29.19511394344499	26.541063696644695	22.610498904201076
28	21.87458784771622	30.06403618802282	28.049926899969414	20.011449064291547
29	21.49870496490918	29.642974399555506	27.220992196115734	21.63732843941958
30	22.47089327956763	29.78819231060638	26.997482889541786	20.743431520284204
31	22.4628957714228	30.36303074691114	25.205900757939283	21.968172723726784
32	23.101994887207074	29.351275813009842	26.78000679086657	20.766722508916523
33	21.76977839887081	30.46306975230174	26.491675576171353	21.2754762726561
34	20.841225611107834	30.0344313771709	28.144213311782153	20.980129699939106
35	22.708573609345578	29.48484822974456	27.994365264436905	19.812212896472957
36	23.15853867286263	31.53024601457511	26.09628999806376	19.214925314498497
37	21.47373029035164	32.77940066393349	25.637204969118393	20.109664076596484
38	21.015767718689755	30.727689056883328	28.0329497335567	20.22359349087021
39	20.931723729588818	30.213323006726323	26.920313951302187	21.934639312382668
40	21.41999264790479	30.561144457446243	27.83581817314465	20.183044721504316
41	20.422408737207494	29.75928903555664	28.857394608276437	20.960907618959425
42	20.772615409654815	30.07554137517854	26.426853668050093	22.724989547116547
43	21.03414795670682	28.766896489795464	28.098473177480138	22.100482376017577
44	21.909804944985563	28.74795502313665	26.646574681292286	22.6956653505855
45	22.020366987408835	29.700360028173677	26.602237618594625	21.677035365822857
46	20.12495755708397	30.65809670530726	28.79060839990908	20.426337337699692
47	21.134046654936988	30.2301598659786	27.79372602501396	20.842067454070452
48	20.962170383403347	28.87465238901002	28.802534508546106	21.360642719040523
49	20.15470267509632	30.12408765268927	27.459794983177172	22.261414689037238
50	20.06392394229447	29.228366740468232	28.728452327836102	21.979256989401197
51	21.08550037742626	27.79919800427095	28.710633318460776	22.404668299842015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9227.0
1	7029.0
2	4831.0
3	3139.0
4	1447.0
5	1279.0
6	1111.0
7	1315.5
8	1520.0
9	1755.5
10	1991.0
11	2315.0
12	2639.0
13	2834.5
14	3030.0
15	2907.0
16	2784.0
17	2849.0
18	2914.0
19	2554.5
20	2195.0
21	2359.5
22	2524.0
23	3989.0
24	5454.0
25	5612.0
26	7024.0
27	8278.0
28	10341.0
29	12404.0
30	14821.0
31	17238.0
32	18952.5
33	20667.0
34	23003.5
35	25340.0
36	28243.5
37	31147.0
38	32281.0
39	33415.0
40	35468.5
41	37522.0
42	39433.0
43	41344.0
44	43026.5
45	44709.0
46	50061.0
47	55413.0
48	53118.0
49	50823.0
50	47618.0
51	44413.0
52	42077.5
53	39742.0
54	36506.0
55	33270.0
56	31522.0
57	29774.0
58	29997.0
59	30220.0
60	28822.5
61	27425.0
62	25087.0
63	22749.0
64	20215.0
65	17681.0
66	15357.5
67	13034.0
68	11116.0
69	9198.0
70	8081.5
71	6965.0
72	5637.0
73	4309.0
74	3634.0
75	2611.5
76	2264.0
77	1791.0
78	1318.0
79	1085.5
80	853.0
81	622.0
82	391.0
83	301.0
84	211.0
85	159.0
86	107.0
87	74.5
88	42.0
89	37.5
90	33.0
91	25.5
92	18.0
93	11.5
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	712722.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.646412807825147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.55619027009412	18.375426427754963
2	8.126751883456304	4.005905634128692
3	3.0788641551557956	2.2764887084155663
4	1.7558279626027917	1.730994431433239
5	1.19559269084527	1.4733535504295499
6	0.9402135441043697	1.3903734681302766
7	0.7573960420989339	1.3066966858808264
8	0.6493847063605648	1.2804002835240635
9	0.5452256727563223	1.2094071303758855
>10	7.151232438316739	38.06032272371967
>50	0.9447864248909819	16.193497120990756
>100	0.28938373546902924	9.912729064114504
>500	0.0062909507710658535	0.9632794474896699
>1k	0.002287618462205765	0.7453646061348221
>5k	5.719046155514412E-4	1.0757607174774892
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7632	1.070824248444695	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1528	0.21438934114563604	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1304	0.18296053720805588	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	1293	0.181417158443264	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1163	0.16317722758663267	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	754	0.10579159896846177	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21481026262694292	0.0
2	0.0	0.0	0.0	0.3065711455518421	0.0
3	0.0	0.0	0.0	0.39005390601103934	0.0
4	0.0	0.0	0.0	0.7972252855952251	0.0
5	0.0	0.0	0.0	0.8767794455622249	0.0
6	0.0	0.0	0.0	1.398862389543188	0.0
7	0.0	0.0	0.0	1.7663268427240917	0.0
8	0.0	0.0	0.0	2.1269162450436494	0.0
9	0.0	0.0	0.0	2.5599041421479902	0.0
10	0.0	0.0	0.0	3.2345009695224785	0.0
11	0.0	0.0	0.0	3.854378004327073	0.0
12	0.0	0.0	0.0	4.139762768653135	0.0
13	0.0	0.0	0.0	4.259725390825595	0.0
14	0.0	0.0	0.0	4.404802994716032	0.0
15	0.0	0.0	0.0	4.5369723398463915	0.0
16	0.0	0.0	0.0	4.767216390121254	0.0
17	0.0	0.0	0.0	5.092448388011034	0.0
18	0.0	0.0	0.0	5.434657552313525	0.0
19	0.0	0.0	0.0	5.6155134821150465	0.0
20	0.0	0.0	0.0	5.802683234136171	0.0
21	0.0	0.0	0.0	6.0336288202132105	0.0
22	0.0	0.0	0.0	6.2655565564133	0.0
23	0.0	0.0	0.0	6.50898947976911	0.0
24	0.0	0.0	0.0	6.706121040181165	0.0
25	0.0	0.0	0.0	6.882066219367439	0.0
26	0.0	0.0	0.0	7.048049590162784	0.0
27	0.0	0.0	0.0	7.22203046910296	0.0
28	0.0	0.0	0.0	7.398536876930978	0.0
29	0.0	0.0	0.0	7.589915843765171	0.0
30	0.0	0.0	0.0	7.834190610083595	0.0
31	0.0	0.0	0.0	8.025850191238659	0.0
32	0.0	0.0	0.0	8.211476564494992	0.0
33	0.0	0.0	0.0	8.414641332805779	0.0
34	0.0	0.0	0.0	8.610369821613476	0.0
35	0.0	0.0	0.0	8.818024419058203	0.0
36	0.0	0.0	0.0	9.018102429839404	0.0
37	0.0	0.0	0.0	9.212427847042745	0.0
38	0.0	0.0	0.0	9.419380908685294	0.0
39	0.0	0.0	0.0	9.641066222173583	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	25	3.8893082E-5	45.000004	1
CATATAG	25	3.8893082E-5	45.000004	10
CTATACG	30	2.1643373E-6	45.000004	1
GCACGAG	30	2.1643373E-6	45.000004	1
TATTCCG	30	2.1643373E-6	45.000004	1
CGTAACG	25	3.8893082E-5	45.000004	1
TCATACG	30	2.1643373E-6	45.000004	1
GTCGACT	20	7.0314814E-4	45.0	27
GACGTAA	20	7.0314814E-4	45.0	33
TAGCGAT	20	7.0314814E-4	45.0	42
ATTACCG	35	1.211065E-7	45.0	1
GTTACGG	20	7.0314814E-4	45.0	2
TACGCAC	20	7.0314814E-4	45.0	32
CGAATCG	20	7.0314814E-4	45.0	1
CATACCG	20	7.0314814E-4	45.0	1
GCGATAT	20	7.0314814E-4	45.0	44
ATACGTA	35	1.211065E-7	45.0	22
ACGAGTC	20	7.0314814E-4	45.0	28
CCGTTGT	20	7.0314814E-4	45.0	40
CCGTTAG	20	7.0314814E-4	45.0	7
>>END_MODULE