FastQCFastQC Report
Sat 18 Jun 2016
SRR3554161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693646
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72931.0514008586512429No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15200.2191319491498545No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT13660.19693042272282982No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12190.17573805658794256No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA11260.16233064127811592No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7360.10610599643045587No Hit
CTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACTC6990.10077186345772916No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGC207.031349E-445.00000430
CGTATAT207.031349E-445.00000430
CTATACG207.031349E-445.0000041
ACCTCGC207.031349E-445.00000412
ATACGTT207.031349E-445.00000439
TAGGACG207.031349E-445.00000424
ATACGCG406.8084773E-945.0000041
TAGCTAC207.031349E-445.00000422
TTTCGTA253.8892E-545.034
TATGCGG253.8892E-545.02
ATCCGTT253.8892E-545.045
CACGTAT351.2109922E-745.034
CTTACCG351.2109922E-745.01
TTATGCA302.1642536E-644.99999638
TCATACG302.1642536E-644.9999961
ACGAACG600.044.9999961
CGACACC650.041.5384634
TAACGGG3800.041.447373
TATACGG603.6379788E-1241.2499962
AAATGCG603.6379788E-1241.2499961