##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693646
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39027544309345	34.0	31.0	34.0	30.0	34.0
2	32.55518232643164	34.0	31.0	34.0	31.0	34.0
3	32.63604057401037	34.0	31.0	34.0	31.0	34.0
4	36.144556445218456	37.0	35.0	37.0	35.0	37.0
5	35.99316365984955	37.0	35.0	37.0	35.0	37.0
6	35.96841184119854	37.0	35.0	37.0	35.0	37.0
7	35.95122151645075	37.0	35.0	37.0	35.0	37.0
8	36.056922407106796	37.0	35.0	37.0	35.0	37.0
9	37.735931584698825	39.0	38.0	39.0	35.0	39.0
10	37.372018291751125	39.0	37.0	39.0	35.0	39.0
11	37.32642731306747	39.0	37.0	39.0	34.0	39.0
12	37.24123976783547	39.0	37.0	39.0	34.0	39.0
13	37.209220553423506	39.0	37.0	39.0	34.0	39.0
14	38.4719482271937	40.0	38.0	41.0	34.0	41.0
15	38.48167220743722	40.0	38.0	41.0	34.0	41.0
16	38.488969878006934	40.0	38.0	41.0	34.0	41.0
17	38.439107267972425	40.0	38.0	41.0	34.0	41.0
18	38.34799451016801	40.0	37.0	41.0	34.0	41.0
19	38.29701029055166	40.0	37.0	41.0	34.0	41.0
20	38.19897036817051	40.0	37.0	41.0	34.0	41.0
21	38.16041612003818	40.0	37.0	41.0	34.0	41.0
22	38.1101138044478	40.0	37.0	41.0	34.0	41.0
23	38.033739111881275	40.0	37.0	41.0	33.0	41.0
24	37.96881694697295	40.0	37.0	41.0	33.0	41.0
25	37.90144252255473	40.0	37.0	41.0	33.0	41.0
26	37.837846682601786	40.0	36.0	41.0	33.0	41.0
27	37.774562240681846	40.0	36.0	41.0	33.0	41.0
28	37.64772376687821	40.0	36.0	41.0	33.0	41.0
29	37.57619016039882	40.0	36.0	41.0	33.0	41.0
30	37.524919050928	40.0	36.0	41.0	33.0	41.0
31	37.44973516750619	40.0	36.0	41.0	33.0	41.0
32	37.38277017383507	40.0	36.0	41.0	33.0	41.0
33	37.31454228814121	39.0	35.0	41.0	32.0	41.0
34	37.19612886111936	39.0	35.0	41.0	32.0	41.0
35	37.10911329410103	39.0	35.0	41.0	32.0	41.0
36	37.02557356346033	39.0	35.0	41.0	31.0	41.0
37	36.902633331699455	39.0	35.0	41.0	31.0	41.0
38	36.84134991047306	39.0	35.0	41.0	31.0	41.0
39	36.77664976082901	39.0	35.0	41.0	31.0	41.0
40	36.66471370122512	39.0	35.0	41.0	31.0	41.0
41	36.60471479688486	39.0	35.0	41.0	31.0	41.0
42	36.51884390596933	39.0	35.0	41.0	31.0	41.0
43	36.42943807071619	39.0	35.0	40.0	30.0	41.0
44	36.33277781462014	39.0	35.0	40.0	30.0	41.0
45	36.18283389509923	38.0	35.0	40.0	30.0	41.0
46	36.04028423720457	38.0	35.0	40.0	30.0	41.0
47	35.91834307413291	38.0	35.0	40.0	29.0	41.0
48	35.79951589139129	38.0	35.0	40.0	29.0	41.0
49	35.68781338031215	38.0	34.0	40.0	29.0	41.0
50	35.58492083858337	38.0	34.0	40.0	28.0	41.0
51	34.56789774611257	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	5.0
13	11.0
14	15.0
15	23.0
16	47.0
17	93.0
18	207.0
19	343.0
20	585.0
21	917.0
22	1414.0
23	1966.0
24	2507.0
25	3426.0
26	4301.0
27	5262.0
28	6518.0
29	8100.0
30	10393.0
31	13667.0
32	18380.0
33	25308.0
34	40657.0
35	53985.0
36	60046.0
37	86480.0
38	141963.0
39	206938.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.89842369162368	31.27070580670832	18.582821785175724	27.248048716492274
2	25.613929872009646	30.140302113758317	19.14319407882407	25.102573935407975
3	22.38591442897386	31.63558933519403	20.200794064984155	25.777702170847956
4	22.089653800353496	32.59068746882415	19.030600623372727	26.28905810744962
5	19.35886604982945	35.10983988951136	16.619572519700252	28.911721540958936
6	21.15171139168971	38.485769398223304	18.599400847117984	21.76311836296901
7	83.42353304134961	7.40377656614469	5.005435049001941	4.167255343503746
8	84.42029507846942	6.969549309013531	4.5723611179189385	4.037794494598109
9	75.93469867915334	11.046268557736944	7.805999025439489	5.213033737670224
10	35.97267193928892	33.92119323112942	14.235359246647425	15.870775582934234
11	26.027973923298052	31.019713225478124	24.61385202250139	18.338460828722432
12	27.369580448816833	26.149938152890666	27.754358851633253	18.726122546659248
13	25.083976552881442	26.99417282014169	26.544952324384486	21.37689830259239
14	21.192942797911325	28.805471378772456	28.08536342745435	21.91622239586187
15	20.24692710691044	30.665642128693886	28.947186316939767	20.140244447455906
16	22.492741254184413	27.985024061264678	27.90847204481825	21.61376263973266
17	21.623565911141995	29.123933533819844	27.47611894251535	21.776381612522812
18	22.515951940903573	28.547847172765355	27.85945568777157	21.076745198559497
19	22.835855753511158	30.26803297359172	26.590364537530668	20.305746735366455
20	24.048145595880317	29.281218373637273	26.789889943861855	19.88074608662055
21	23.218183338475246	29.983593936964965	26.06733117469141	20.730891549868378
22	22.158276700218842	29.335857195168717	26.314431280509076	22.19143482410336
23	22.38000363297705	29.44369318067141	26.13941405270123	22.036889133650305
24	21.5010250185253	28.879572577366552	27.922167791640117	21.69723461246803
25	21.696802115199972	31.04941137121817	25.80754448234402	21.446242031237837
26	22.47010723048933	30.04587354356545	25.973479267522627	21.510539958422594
27	21.679358058721597	29.075782171309285	26.59079703479873	22.65406273517039
28	21.845292843900204	30.035061111863975	28.121693197971297	19.99795284626452
29	21.575270382875413	29.721500592521256	27.243867909567705	21.459361115035623
30	22.305469937114896	29.826309097147536	26.94933727001958	20.918883695717987
31	22.40393514847631	30.43886939447499	25.21718571144359	21.940009745605106
32	23.064935139826368	29.240851961951776	26.913439996770688	20.780772901451172
33	21.66652730643585	30.603795019361463	26.580705431877355	21.148972242325335
34	20.795910305833235	30.026411166502797	28.211940961239595	20.965737566424373
35	22.700339942852693	29.43561989833431	28.02120966602561	19.842830492787385
36	23.143505476857072	31.53568246627242	26.055077085429744	19.265734971440764
37	21.470894375517194	32.82697514294035	25.539972839171565	20.162157642370893
38	20.997454032748696	30.70918018701182	28.12544150762781	20.16792427261168
39	20.919027861474007	30.183984337832264	26.96447467440164	21.932513126292086
40	21.355994267969542	30.56054529255557	27.93355688636567	20.149903553109223
41	20.51694956793523	29.745576273776535	28.726324378717678	21.01114977957056
42	20.890915539050177	30.047171035369622	26.431493874397027	22.630419551183167
43	21.000769845137146	28.97356865029136	28.04139287186836	21.984268632703134
44	22.00286601522967	28.63261663730491	26.69820052303336	22.66631682443206
45	21.936549767460637	29.832075727388325	26.54855646828497	21.682818036866067
46	20.08993059860505	30.625131551252366	28.850451094650587	20.434486755491996
47	21.127924041946468	30.21440331235241	27.813178480089267	20.844494165611856
48	20.89351052265853	28.80028141155575	28.937383045530428	21.36882502025529
49	20.229338884676046	29.995559694714597	27.557716760422462	22.217384660186895
50	20.057493303500635	29.253106051213447	28.703257857754533	21.986142787531392
51	21.061463628421414	27.835812503784354	28.716809438820377	22.38591442897386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8838.0
1	6800.0
2	4762.0
3	3065.0
4	1368.0
5	1243.5
6	1119.0
7	1347.0
8	1575.0
9	1786.0
10	1997.0
11	2281.5
12	2566.0
13	2797.5
14	3029.0
15	2913.5
16	2798.0
17	2819.5
18	2841.0
19	2501.5
20	2162.0
21	2310.5
22	2459.0
23	3783.5
24	5108.0
25	5282.5
26	6624.5
27	7792.0
28	9856.0
29	11920.0
30	14300.0
31	16680.0
32	18573.5
33	20467.0
34	22661.5
35	24856.0
36	27618.5
37	30381.0
38	31579.0
39	32777.0
40	34578.0
41	36379.0
42	38282.5
43	40186.0
44	41794.5
45	43403.0
46	48828.5
47	54254.0
48	51718.0
49	49182.0
50	46377.0
51	43572.0
52	41015.5
53	38459.0
54	35415.5
55	32372.0
56	30696.0
57	29020.0
58	29229.0
59	29438.0
60	28170.0
61	26902.0
62	24479.0
63	22056.0
64	19703.0
65	17350.0
66	14933.0
67	12516.0
68	10671.0
69	8826.0
70	7796.0
71	6766.0
72	5531.0
73	4296.0
74	3572.0
75	2545.5
76	2243.0
77	1717.5
78	1192.0
79	974.5
80	757.0
81	523.0
82	289.0
83	231.0
84	173.0
85	142.0
86	111.0
87	80.5
88	50.0
89	37.0
90	24.0
91	22.5
92	21.0
93	12.5
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	693646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.273495126108454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7530835230393	17.90245113431964
2	8.380648798634235	4.068552755345496
3	3.0969340578686397	2.255202412786611
4	1.7712419260870096	1.7197692904012791
5	1.2819020760290514	1.5558121897319743
6	0.9599151358537678	1.3980297222974496
7	0.8113172670060889	1.378545400847995
8	0.6884427159774076	1.3368728728705974
9	0.6085336940833794	1.3294115691665114
>10	7.408027165506784	38.74974781499036
>50	0.9354874115290269	15.742838045718855
>100	0.29610833191984515	9.930169185008285
>500	0.005372933442093965	0.8177326082217224
>1k	0.0023879704187084287	0.758029288252318
>5k	5.969926046771072E-4	1.0568357100409396
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7293	1.0514008586512429	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1520	0.2191319491498545	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	1366	0.19693042272282982	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1219	0.17573805658794256	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1126	0.16233064127811592	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	736	0.10610599643045587	No Hit
CTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACTC	699	0.10077186345772916	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.883315120392823E-4	0.0	0.0	0.22014110944199203	0.0
2	2.883315120392823E-4	0.0	0.0	0.3210571386557408	0.0
3	2.883315120392823E-4	0.0	0.0	0.41433238280044865	0.0
4	2.883315120392823E-4	0.0	0.0	0.8249164559443867	0.0
5	2.883315120392823E-4	0.0	0.0	0.9089650917038374	0.0
6	2.883315120392823E-4	0.0	0.0	1.4406483999042738	0.0
7	2.883315120392823E-4	0.0	0.0	1.7984678063450232	0.0
8	2.883315120392823E-4	0.0	0.0	2.158882196394126	0.0
9	2.883315120392823E-4	0.0	0.0	2.6158876429763884	0.0
10	2.883315120392823E-4	0.0	0.0	3.312352410307275	0.0
11	2.883315120392823E-4	0.0	0.0	3.9452400792334994	0.0
12	2.883315120392823E-4	0.0	0.0	4.2324182652246245	0.0
13	2.883315120392823E-4	0.0	0.0	4.359428296277929	0.0
14	2.883315120392823E-4	0.0	0.0	4.5048915441017465	0.0
15	2.883315120392823E-4	0.0	0.0	4.6398306917361305	0.0
16	2.883315120392823E-4	0.0	0.0	4.894427416866817	0.0
17	2.883315120392823E-4	0.0	0.0	5.215052058254499	0.0
18	2.883315120392823E-4	0.0	0.0	5.556869065777068	0.0
19	2.883315120392823E-4	0.0	0.0	5.741545399238228	0.0
20	2.883315120392823E-4	0.0	0.0	5.933430020500371	0.0
21	2.883315120392823E-4	0.0	0.0	6.1519853066261465	0.0
22	2.883315120392823E-4	0.0	0.0	6.40773535780499	0.0
23	2.883315120392823E-4	0.0	0.0	6.66867537620054	0.0
24	2.883315120392823E-4	0.0	0.0	6.870074937359979	0.0
25	2.883315120392823E-4	0.0	0.0	7.065275371010573	0.0
26	2.883315120392823E-4	0.0	0.0	7.233949305553553	0.0
27	2.883315120392823E-4	0.0	0.0	7.412426511505869	0.0
28	2.883315120392823E-4	0.0	0.0	7.590471220190126	0.0
29	2.883315120392823E-4	0.0	0.0	7.7891316319851915	0.0
30	2.883315120392823E-4	0.0	0.0	8.029023450001874	0.0
31	2.883315120392823E-4	0.0	0.0	8.219754745215859	0.0
32	2.883315120392823E-4	0.0	0.0	8.421154306375298	0.0
33	2.883315120392823E-4	0.0	0.0	8.62500468538707	0.0
34	2.883315120392823E-4	0.0	0.0	8.823376765670098	0.0
35	2.883315120392823E-4	0.0	0.0	9.03501209550693	0.0
36	2.883315120392823E-4	0.0	0.0	9.243475778711332	0.0
37	2.883315120392823E-4	0.0	0.0	9.446028665918927	0.0
38	2.883315120392823E-4	0.0	0.0	9.644689077713993	0.0
39	2.883315120392823E-4	0.0	0.0	9.871894309200947	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTGC	20	7.031349E-4	45.000004	30
CGTATAT	20	7.031349E-4	45.000004	30
CTATACG	20	7.031349E-4	45.000004	1
ACCTCGC	20	7.031349E-4	45.000004	12
ATACGTT	20	7.031349E-4	45.000004	39
TAGGACG	20	7.031349E-4	45.000004	24
ATACGCG	40	6.8084773E-9	45.000004	1
TAGCTAC	20	7.031349E-4	45.000004	22
TTTCGTA	25	3.8892E-5	45.0	34
TATGCGG	25	3.8892E-5	45.0	2
ATCCGTT	25	3.8892E-5	45.0	45
CACGTAT	35	1.2109922E-7	45.0	34
CTTACCG	35	1.2109922E-7	45.0	1
TTATGCA	30	2.1642536E-6	44.999996	38
TCATACG	30	2.1642536E-6	44.999996	1
ACGAACG	60	0.0	44.999996	1
CGACACC	65	0.0	41.53846	34
TAACGGG	380	0.0	41.44737	3
TATACGG	60	3.6379788E-12	41.249996	2
AAATGCG	60	3.6379788E-12	41.249996	1
>>END_MODULE