FastQCFastQC Report
Sat 18 Jun 2016
SRR3554160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003879
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82810.824900212077352No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA17210.17143500362095432No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16020.1595809853577971No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT15650.15589528220034485No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14120.1406544015762856No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13630.13577333523263263No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10980.10937573153736657No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT10590.10549080118221418No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC207.0328586E-445.00000410
ACTATCG207.0328586E-445.00000442
TCGCACA207.0328586E-445.00000425
CTATCGT207.0328586E-445.00000443
TCCGAAC207.0328586E-445.00000440
TATATCG207.0328586E-445.00000428
CGTTAGT207.0328586E-445.00000441
TACGACG351.2117198E-745.0000041
TCCCGAT207.0328586E-445.00000440
ACGTCGA207.0328586E-445.00000442
ACTCACG351.2117198E-745.0000041
CCGCCCA207.0328586E-445.00000436
GATAGCG207.0328586E-445.0000041
CGTATCG253.8904524E-545.043
ACATCCG253.8904524E-545.01
TCAACCG502.1827873E-1145.01
ATTTACG302.1652268E-644.9999961
CGACACT302.1652268E-644.99999634
CCGAACG302.1652268E-644.9999961
ACTACGG1550.042.096772