##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1003879
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.253596299952484	34.0	31.0	34.0	30.0	34.0
2	32.44140877536038	34.0	31.0	34.0	30.0	34.0
3	32.52460406084797	34.0	31.0	34.0	30.0	34.0
4	36.0594334576179	37.0	35.0	37.0	35.0	37.0
5	35.893500113061435	37.0	35.0	37.0	35.0	37.0
6	35.863831198779934	37.0	35.0	37.0	35.0	37.0
7	35.92236713787219	37.0	35.0	37.0	35.0	37.0
8	36.06079019483424	37.0	35.0	37.0	35.0	37.0
9	37.69970185649864	39.0	38.0	39.0	35.0	39.0
10	37.267438605648685	39.0	37.0	39.0	34.0	39.0
11	37.20445392323178	39.0	37.0	39.0	34.0	39.0
12	37.13609010647698	39.0	37.0	39.0	34.0	39.0
13	37.11355053746517	39.0	37.0	39.0	33.0	39.0
14	38.35450886013155	40.0	38.0	41.0	34.0	41.0
15	38.347527939124134	40.0	38.0	41.0	34.0	41.0
16	38.34003699649061	40.0	38.0	41.0	34.0	41.0
17	38.29898125172456	40.0	38.0	41.0	34.0	41.0
18	38.230842561703156	40.0	37.0	41.0	34.0	41.0
19	38.21265411468912	40.0	37.0	41.0	34.0	41.0
20	38.124510025610654	40.0	37.0	41.0	33.0	41.0
21	38.08467753583848	40.0	37.0	41.0	33.0	41.0
22	38.01973046552423	40.0	37.0	41.0	33.0	41.0
23	37.942554829815144	40.0	37.0	41.0	33.0	41.0
24	37.868501084294024	40.0	37.0	41.0	33.0	41.0
25	37.80097302563357	40.0	37.0	41.0	33.0	41.0
26	37.71707546427408	40.0	36.0	41.0	33.0	41.0
27	37.65710210095041	40.0	36.0	41.0	33.0	41.0
28	37.55396317683705	40.0	36.0	41.0	33.0	41.0
29	37.44050926456276	40.0	36.0	41.0	32.0	41.0
30	37.41340639658764	40.0	36.0	41.0	32.0	41.0
31	37.3450366030169	40.0	36.0	41.0	32.0	41.0
32	37.29817836611783	39.0	36.0	41.0	32.0	41.0
33	37.205255812702525	39.0	35.0	41.0	32.0	41.0
34	37.07584977870839	39.0	35.0	41.0	31.0	41.0
35	37.04151695572873	39.0	35.0	41.0	31.0	41.0
36	36.9041667372263	39.0	35.0	41.0	31.0	41.0
37	36.83123962150817	39.0	35.0	41.0	31.0	41.0
38	36.756920903814105	39.0	35.0	41.0	31.0	41.0
39	36.69321003826158	39.0	35.0	41.0	31.0	41.0
40	36.60373610763847	39.0	35.0	41.0	30.0	41.0
41	36.515966565691684	39.0	35.0	41.0	30.0	41.0
42	36.39185100993247	39.0	35.0	40.0	30.0	41.0
43	36.3101529168356	39.0	35.0	40.0	30.0	41.0
44	36.1972717827547	39.0	35.0	40.0	30.0	41.0
45	36.05354230938191	38.0	35.0	40.0	29.0	41.0
46	35.93039400166753	38.0	35.0	40.0	29.0	41.0
47	35.76225521203253	38.0	35.0	40.0	28.0	41.0
48	35.654822941808725	38.0	34.0	40.0	28.0	41.0
49	35.50019673685773	38.0	34.0	40.0	28.0	41.0
50	35.37505715330234	38.0	34.0	40.0	27.0	41.0
51	34.351261456809034	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	7.0
12	6.0
13	7.0
14	28.0
15	43.0
16	80.0
17	155.0
18	305.0
19	541.0
20	914.0
21	1479.0
22	2176.0
23	2986.0
24	4073.0
25	5180.0
26	6629.0
27	8385.0
28	10496.0
29	13253.0
30	17264.0
31	22081.0
32	28474.0
33	38218.0
34	58484.0
35	76568.0
36	86079.0
37	123842.0
38	203630.0
39	292395.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.6110706569218	31.096775607418824	19.888751532804253	27.403402202855126
2	25.138188965004748	29.853000212176966	19.405027896788358	25.60378292602993
3	21.61196717931145	31.528999012829235	20.121150058921444	26.73788374893787
4	22.176875898390144	31.99499142824982	18.87847041326694	26.9496622600931
5	19.21436746858934	34.45504886545091	16.77682270472836	29.553760961231383
6	20.59052933670293	38.77050919483324	18.689005348254124	21.949956120209706
7	84.49016265904557	6.924041642468863	4.638208389656522	3.947587308829052
8	86.18688108825864	6.034193363941272	4.133466284283265	3.6454592635168184
9	77.44409435798538	10.890256694282877	6.869154549502481	4.796494398229269
10	33.49298072775703	36.38326929839154	14.168341005240674	15.95540896861076
11	25.438025897543433	30.352562410410023	26.619742020701697	17.589669671344854
12	26.01498786208298	26.37688406670525	29.76334797321191	17.84478009799986
13	24.351839215682368	27.670366647773285	27.575036433673777	20.402757702870566
14	20.628880572260204	29.70039217873867	28.44964383157731	21.221083417423813
15	20.367096034482245	31.026548020229527	28.836742276708648	19.76961366857958
16	21.714668799725864	29.687243183690466	27.963828309985566	20.634259706598108
17	20.69004332195414	29.56581420669224	28.884756031354375	20.85938643999924
18	21.575010534138077	30.00670399520261	27.37551039517711	21.042775075482204
19	21.851338657348148	30.247968131617455	27.67923225807094	20.221460952963454
20	22.85584218815216	29.79602123363473	27.418045401886083	19.93009117632703
21	23.30579681415788	30.426674927954465	27.062524467590215	19.205003790297436
22	21.863491516407855	29.022222797767462	27.245315421480075	21.868970264344608
23	21.23423241247202	30.38394069404779	27.363058695320852	21.01876819815934
24	20.880006454961205	29.589621856817406	28.685130379258855	20.845241308962535
25	20.80868311818456	31.54912095979695	25.632870096894152	22.009325825124343
26	22.31842682235608	29.907887305143348	26.724734753889663	21.04895111861091
27	21.54492722728536	28.835746140720147	27.224795020116964	22.39453161187753
28	20.81724988768567	31.035015176131786	28.359294297420305	19.788440638762243
29	21.3619370461978	30.712167502258737	26.1320338407318	21.793861610811664
30	22.341437563690445	29.73216891677184	27.61159462445175	20.31479889508596
31	22.559093277177826	30.322479103557303	25.973648218560204	21.144779400704667
32	22.08732327302394	30.128531426596233	27.556508304287668	20.22763699609216
33	20.82681279317527	30.51662600771607	27.42302608182859	21.23353511728007
34	20.55765684908241	30.22605313987044	28.135860995199618	21.08042901584753
35	22.812510272652382	29.343974722053158	28.522959440330958	19.320555564963506
36	22.350801241982353	32.362764835204246	26.288526804525247	18.99790711828816
37	20.514922615175735	32.74089805643907	27.1134270166026	19.630752311782594
38	20.39917161331196	30.754005213775766	28.683337334479553	20.163485838432717
39	20.546998194005454	29.638432520253932	27.886926611673317	21.927642674067293
40	22.158447382602883	29.91864557381916	28.292453572591914	19.630453470986044
41	20.164980042415472	28.85576847408901	29.949725016660373	21.029526466835147
42	21.747043219352133	30.610362404233975	26.480681436707016	21.161912939706877
43	21.039388213121303	29.82391304131275	27.78691455842786	21.34978418713809
44	22.046979765489667	29.57916242893815	26.85283784201084	21.521019963561344
45	21.598618957065543	29.58374465448525	28.021903038115152	20.795733350334054
46	20.026915594409285	30.25563837872891	28.400235486547682	21.31721054031412
47	21.09905675883249	30.21091187284523	28.62287187997757	20.06715948834471
48	20.862972529557844	30.25065769878641	28.502439038967843	20.383930732687904
49	20.296270765699852	30.903027157655455	28.03325898838406	20.767443088260638
50	19.789436774750744	29.457434611143373	29.343775494855457	21.409353119250426
51	20.368490624866144	29.00708153074225	28.88734598492448	21.73708185946713
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9753.0
1	7580.5
2	5408.0
3	3572.0
4	1736.0
5	1632.0
6	1528.0
7	1605.5
8	1683.0
9	1836.0
10	1989.0
11	2363.5
12	2738.0
13	3175.5
14	3613.0
15	3579.5
16	3546.0
17	3433.0
18	3320.0
19	3721.5
20	4123.0
21	4515.5
22	4908.0
23	6202.0
24	7496.0
25	8921.5
26	12508.5
27	14670.0
28	17428.0
29	20186.0
30	23983.0
31	27780.0
32	30909.5
33	34039.0
34	37503.0
35	40967.0
36	43739.0
37	46511.0
38	48623.0
39	50735.0
40	53990.0
41	57245.0
42	60099.0
43	62953.0
44	66274.0
45	69595.0
46	71231.5
47	72868.0
48	73087.0
49	73306.0
50	69138.5
51	64971.0
52	60895.5
53	56820.0
54	52097.0
55	47374.0
56	43037.5
57	38701.0
58	36433.0
59	34165.0
60	33656.0
61	33147.0
62	29975.5
63	26804.0
64	23663.0
65	20522.0
66	17735.5
67	14949.0
68	12819.0
69	10689.0
70	9346.0
71	8003.0
72	6827.5
73	5652.0
74	4718.5
75	3117.5
76	2450.0
77	1786.5
78	1123.0
79	916.0
80	709.0
81	616.5
82	524.0
83	385.5
84	247.0
85	162.5
86	78.0
87	67.0
88	56.0
89	47.0
90	38.0
91	27.5
92	17.0
93	11.5
94	6.0
95	5.5
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1003879.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.82785535169201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23612251150527	17.174793219148214
2	8.60373404608976	3.9280959257712973
3	3.1255956828695517	2.140519384092574
4	1.684432194800627	1.5380789797056724
5	1.127056633097178	1.2864142896753696
6	0.8027073735057143	1.0994452687275034
7	0.6476128491021692	1.0348528710241023
8	0.5303563494608764	0.9685518424275446
9	0.469153504445019	0.963879150348926
>10	6.154992195159088	32.15946441255884
>50	1.088321134045121	17.698099812536476
>100	0.5233408916029858	17.637365494303296
>500	0.0030681626810895317	0.559316025693837
>1k	0.0030681626810895317	0.9825551649934668
>5k	4.383089544413616E-4	0.8285681589929632
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8281	0.824900212077352	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1721	0.17143500362095432	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1602	0.1595809853577971	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	1565	0.15589528220034485	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1412	0.1406544015762856	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1363	0.13577333523263263	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1098	0.10937573153736657	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	1059	0.10549080118221418	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.961359885006061E-5	0.0	0.0	0.19066042819901602	0.0
2	9.961359885006061E-5	0.0	0.0	0.27154667046526526	0.0
3	9.961359885006061E-5	0.0	0.0	0.358210501464818	0.0
4	9.961359885006061E-5	0.0	0.0	0.7559676016731101	0.0
5	9.961359885006061E-5	0.0	0.0	0.8201187593325491	0.0
6	9.961359885006061E-5	0.0	0.0	1.2826246987933805	0.0
7	9.961359885006061E-5	0.0	0.0	1.602483964700925	0.0
8	9.961359885006061E-5	0.0	0.0	1.8984359668844553	0.0
9	9.961359885006061E-5	0.0	0.0	2.290913546353694	0.0
10	9.961359885006061E-5	0.0	0.0	2.916686174329775	0.0
11	9.961359885006061E-5	0.0	0.0	3.4759169182740153	0.0
12	9.961359885006061E-5	0.0	0.0	3.741785613604827	0.0
13	9.961359885006061E-5	0.0	0.0	3.8645095673881014	0.0
14	9.961359885006061E-5	0.0	0.0	3.9970952674575324	0.0
15	9.961359885006061E-5	0.0	0.0	4.12479990118331	0.0
16	9.961359885006061E-5	0.0	0.0	4.382201440611866	0.0
17	9.961359885006061E-5	0.0	0.0	4.688314029878103	0.0
18	9.961359885006061E-5	0.0	0.0	5.074914407015188	0.0
19	9.961359885006061E-5	0.0	0.0	5.24166757149019	0.0
20	9.961359885006061E-5	0.0	0.0	5.432427613288056	0.0
21	9.961359885006061E-5	0.0	0.0	5.642612306861683	0.0
22	9.961359885006061E-5	0.0	0.0	5.901906504668391	0.0
23	9.961359885006061E-5	0.0	0.0	6.161300316073949	0.0
24	9.961359885006061E-5	0.0	0.0	6.355546833831568	0.0
25	9.961359885006061E-5	0.0	0.0	6.539333923709929	0.0
26	9.961359885006061E-5	0.0	0.0	6.697918773079226	0.0
27	9.961359885006061E-5	0.0	0.0	6.879514363782886	0.0
28	9.961359885006061E-5	0.0	0.0	7.070374019179602	0.0
29	9.961359885006061E-5	0.0	0.0	7.261632128971718	0.0
30	9.961359885006061E-5	0.0	0.0	7.508275399724469	0.0
31	9.961359885006061E-5	0.0	0.0	7.705609939046439	0.0
32	9.961359885006061E-5	0.0	0.0	7.908024771909762	0.0
33	9.961359885006061E-5	0.0	0.0	8.11751217029144	0.0
34	9.961359885006061E-5	0.0	0.0	8.319229707962812	0.0
35	9.961359885006061E-5	0.0	0.0	8.544356441363949	0.0
36	9.961359885006061E-5	0.0	0.0	8.747966637413473	0.0
37	9.961359885006061E-5	0.0	0.0	8.95665712700435	0.0
38	9.961359885006061E-5	0.0	0.0	9.183776132382489	0.0
39	9.961359885006061E-5	0.0	0.0	9.43251128871109	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	20	7.0328586E-4	45.000004	10
ACTATCG	20	7.0328586E-4	45.000004	42
TCGCACA	20	7.0328586E-4	45.000004	25
CTATCGT	20	7.0328586E-4	45.000004	43
TCCGAAC	20	7.0328586E-4	45.000004	40
TATATCG	20	7.0328586E-4	45.000004	28
CGTTAGT	20	7.0328586E-4	45.000004	41
TACGACG	35	1.2117198E-7	45.000004	1
TCCCGAT	20	7.0328586E-4	45.000004	40
ACGTCGA	20	7.0328586E-4	45.000004	42
ACTCACG	35	1.2117198E-7	45.000004	1
CCGCCCA	20	7.0328586E-4	45.000004	36
GATAGCG	20	7.0328586E-4	45.000004	1
CGTATCG	25	3.8904524E-5	45.0	43
ACATCCG	25	3.8904524E-5	45.0	1
TCAACCG	50	2.1827873E-11	45.0	1
ATTTACG	30	2.1652268E-6	44.999996	1
CGACACT	30	2.1652268E-6	44.999996	34
CCGAACG	30	2.1652268E-6	44.999996	1
ACTACGG	155	0.0	42.09677	2
>>END_MODULE