FastQCFastQC Report
Sat 18 Jun 2016
SRR3554158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082389
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67670.625191128143394No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA21400.19771080452591444No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA17400.16075551396032295No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14870.13738129267758634No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14260.13174561086633363No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12530.11576244769671531No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG11220.1036595900364841No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG207.0331036E-445.0000041
AATTACG551.8189894E-1245.0000041
ATACGCG406.8139343E-945.0000041
TAATACG453.8562575E-1045.01
ATTCGCT253.890655E-545.013
ACGATAG351.2118471E-745.01
CGCTATA253.890655E-545.024
TCGACAA253.890655E-545.014
GTGCGAT253.890655E-545.033
TAAACGG1700.042.352942
TCGGGAT1850.041.3513535
TTACGAG556.184564E-1140.9090921
CGAACCG501.0822987E-940.51
AGATACG451.9281288E-840.01
CCTCGCG451.9281288E-840.01
CTTACCG451.9281288E-840.01
CGCTAAT403.4579716E-739.37500422
GTAACGG1550.039.1935462
CATACGA4250.038.6470618
TAGCACG356.247952E-638.5714261