##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1082389
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27999730226379	34.0	31.0	34.0	30.0	34.0
2	32.46316158054082	34.0	31.0	34.0	30.0	34.0
3	32.55992623724003	34.0	31.0	34.0	31.0	34.0
4	36.07887275277188	37.0	35.0	37.0	35.0	37.0
5	35.90890520875582	37.0	35.0	37.0	35.0	37.0
6	35.88377006787763	37.0	35.0	37.0	35.0	37.0
7	35.91108187537013	37.0	35.0	37.0	35.0	37.0
8	36.01265071984287	37.0	35.0	37.0	35.0	37.0
9	37.64654666667899	39.0	37.0	39.0	35.0	39.0
10	37.270874888787674	39.0	37.0	39.0	34.0	39.0
11	37.19464074376218	39.0	37.0	39.0	34.0	39.0
12	37.086868953768004	39.0	37.0	39.0	33.0	39.0
13	37.04859990262281	39.0	37.0	39.0	33.0	39.0
14	38.298734558462805	40.0	38.0	41.0	33.0	41.0
15	38.3074633980944	40.0	38.0	41.0	33.0	41.0
16	38.29685168640849	40.0	38.0	41.0	33.0	41.0
17	38.26566234505339	40.0	38.0	41.0	34.0	41.0
18	38.19130830043542	40.0	37.0	41.0	33.0	41.0
19	38.17818732452011	40.0	37.0	41.0	34.0	41.0
20	38.1002430734237	40.0	37.0	41.0	33.0	41.0
21	38.031352868515846	40.0	37.0	41.0	33.0	41.0
22	37.951352055499456	40.0	37.0	41.0	33.0	41.0
23	37.89317056991525	40.0	37.0	41.0	33.0	41.0
24	37.81269118588604	40.0	36.0	41.0	33.0	41.0
25	37.738919187094474	40.0	36.0	41.0	33.0	41.0
26	37.64938206134763	40.0	36.0	41.0	33.0	41.0
27	37.5691013120052	40.0	36.0	41.0	33.0	41.0
28	37.49216686422349	40.0	36.0	41.0	33.0	41.0
29	37.354831765659114	39.0	36.0	41.0	32.0	41.0
30	37.334751184648034	39.0	36.0	41.0	32.0	41.0
31	37.24759767514267	39.0	35.0	41.0	32.0	41.0
32	37.18888772890338	39.0	35.0	41.0	32.0	41.0
33	37.08367139725182	39.0	35.0	41.0	31.0	41.0
34	36.95132803456059	39.0	35.0	41.0	31.0	41.0
35	36.90995104347882	39.0	35.0	41.0	31.0	41.0
36	36.78221692940338	39.0	35.0	41.0	31.0	41.0
37	36.68148050285064	39.0	35.0	41.0	31.0	41.0
38	36.61361118784467	39.0	35.0	41.0	30.0	41.0
39	36.538227014502176	39.0	35.0	41.0	30.0	41.0
40	36.41388170057161	39.0	35.0	41.0	30.0	41.0
41	36.29391466469079	39.0	35.0	40.0	30.0	41.0
42	36.17855502966124	38.0	35.0	40.0	30.0	41.0
43	36.098811055914275	38.0	35.0	40.0	29.0	41.0
44	35.98952687065371	38.0	35.0	40.0	29.0	41.0
45	35.84265545935888	38.0	35.0	40.0	29.0	41.0
46	35.733395295037184	38.0	34.0	40.0	28.0	41.0
47	35.57047604881424	38.0	34.0	40.0	28.0	41.0
48	35.46731350743587	38.0	34.0	40.0	28.0	41.0
49	35.295281086559456	38.0	34.0	40.0	27.0	41.0
50	35.16222633452483	37.0	34.0	40.0	27.0	41.0
51	34.10491514603345	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	11.0
11	9.0
12	10.0
13	22.0
14	43.0
15	45.0
16	96.0
17	194.0
18	383.0
19	682.0
20	1043.0
21	1608.0
22	2319.0
23	3362.0
24	4594.0
25	6043.0
26	7771.0
27	9600.0
28	11791.0
29	14996.0
30	19216.0
31	24600.0
32	31313.0
33	42382.0
34	66957.0
35	83894.0
36	94430.0
37	135945.0
38	215526.0
39	303393.0
40	102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.502815531199964	31.565361436599964	18.6538296305672	27.277993401632873
2	24.92320228679338	30.587524448234415	18.946238367167442	25.543034897804763
3	22.55205845587862	30.000489657599992	19.692827624818804	27.75462426170259
4	22.829500299799797	31.72426918603201	18.32344933291081	27.122781181257384
5	20.090466551304566	33.90232162374156	16.313913020180358	29.693298804773516
6	20.814420693484507	38.995222604812135	18.710186448679725	21.480170253023637
7	83.57189513197196	7.045156593424361	5.246912154502679	4.136036120100998
8	84.51758101754545	6.49831068128002	4.907939751789791	4.07616854938474
9	75.75030788376452	10.717865758059256	8.025857616808745	5.505968741367475
10	36.427569016314834	33.30004277574883	14.550683719069577	15.721704488866756
11	27.39588077853711	31.050574239021277	24.080898826577133	17.47264615586448
12	28.236613638904313	25.494069137805354	27.90493990607813	18.364377317212206
13	24.485836422949607	27.50776292072443	26.706664609488822	21.299736046837136
14	21.7912414113595	28.55091838516467	27.45750372555523	22.200336477920597
15	21.557499198532135	29.666783383792705	28.295002997997948	20.480714419677216
16	22.872645601535122	28.513131600561355	27.678403974911053	20.935818822992474
17	21.96668665331965	27.97995914592628	28.493914849467245	21.55943935128683
18	22.764736153083597	28.79205165610515	27.099406959974647	21.343805230836605
19	22.68916258387696	29.410683220173155	26.234930325419047	21.665223870530834
20	24.674493181286948	28.52920715195738	26.490476159680114	20.30582350707555
21	24.40305657208268	28.988746190140514	25.685035601803047	20.92316163597376
22	23.128930541607502	28.50786547165575	26.27724413311665	22.085959853620093
23	23.486011036697526	29.153104844930983	25.948157270630062	21.412726847741432
24	21.604155252871195	28.93211220734874	27.734206463665096	21.729526076114965
25	22.30390367973067	29.64876767964198	25.559941943238524	22.487386697388832
26	23.393715198509963	29.36254895421147	25.301532073958622	21.942203773319942
27	22.235074451052256	27.73429885189151	26.21913193870226	23.811494758353973
28	21.789578423284052	29.97092542514752	27.184034575369854	21.055461576198574
29	21.90986789407505	30.19182567450334	25.38486625418403	22.513440177237573
30	23.06047086583474	28.810714077840778	26.48761212466128	21.6412029316632
31	23.13706070553193	29.41567218439951	25.181150214941212	22.266116895127354
32	23.071927005910077	28.375473143204523	26.795542083299072	21.75705776758633
33	21.880673214528233	29.759079221980265	26.025670992591387	22.33457657090011
34	21.04539125951945	29.39229796311677	27.860870722078662	21.701440055285115
35	22.777300951875898	29.036603291422953	27.108368617936808	21.07772713876434
36	22.54984113844468	32.70321483311452	24.527688289515137	20.219255738925654
37	20.884081416200644	32.57728968051227	25.56086582550266	20.97776307778442
38	21.068211151443705	30.48774516370732	27.06106584601285	21.38297783883613
39	21.654322059813985	29.479697225304395	26.216729844815497	22.649250870066123
40	22.562590713689808	29.697086722056486	26.753874993186365	20.986447571067334
41	20.3940542633009	29.158832914968645	28.877603153764497	21.569509667965953
42	21.225271136347466	29.482006930964744	26.315585247078456	22.977136685609334
43	21.35138106540255	29.50648981096445	26.968585231372455	22.173543892260547
44	22.127072614374317	28.530592975353592	26.24694079485287	23.095393615419223
45	22.17964151520387	28.86817955467027	27.229581970991944	21.722596959133917
46	20.355805537565516	29.613844930057493	27.923417551360924	22.106931981016068
47	20.875489311144147	30.405889195104535	27.341741277858517	21.376880215892808
48	20.98589324170885	28.74899874259624	28.5365982100705	21.72850980562441
49	21.125584240046784	30.33613608416198	26.919804247825873	21.61847542796536
50	20.66031713182599	29.194587158590856	28.071146325396878	22.073949384186275
51	21.0913082080472	28.22765198094216	28.134247484037623	22.54679232697302
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8266.0
1	6640.0
2	5014.0
3	3387.0
4	1760.0
5	1670.0
6	1580.0
7	1829.5
8	2079.0
9	2304.0
10	2529.0
11	2948.0
12	3367.0
13	3737.5
14	4108.0
15	3970.5
16	3833.0
17	4071.0
18	4309.0
19	4338.0
20	4367.0
21	4701.0
22	5035.0
23	7041.0
24	9047.0
25	8957.0
26	10328.5
27	11790.0
28	15273.0
29	18756.0
30	21784.0
31	24812.0
32	28492.5
33	32173.0
34	37219.5
35	42266.0
36	44131.5
37	45997.0
38	47372.0
39	48747.0
40	51714.5
41	54682.0
42	58537.5
43	62393.0
44	64225.5
45	66058.0
46	70712.5
47	75367.0
48	75251.5
49	75136.0
50	71495.5
51	67855.0
52	64502.0
53	61149.0
54	57104.5
55	53060.0
56	51833.5
57	50607.0
58	47925.5
59	45244.0
60	43996.5
61	42749.0
62	40587.5
63	38426.0
64	33589.0
65	28752.0
66	25075.5
67	21399.0
68	18881.5
69	16364.0
70	14164.0
71	11964.0
72	10139.0
73	8314.0
74	6985.5
75	4864.5
76	4072.0
77	3157.0
78	2242.0
79	1646.5
80	1051.0
81	781.5
82	512.0
83	379.0
84	246.0
85	212.0
86	178.0
87	133.5
88	89.0
89	82.5
90	76.0
91	48.5
92	21.0
93	16.0
94	11.0
95	10.0
96	9.0
97	5.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1082389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.649868585163134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09176273594869	17.00818557797488
2	8.048975034376216	3.646164535477604
3	2.9444890421900656	2.0007686956817357
4	1.6456487650488758	1.4909491306277218
5	1.073511264930726	1.2157444537686595
6	0.8415718827228534	1.1436895529184574
7	0.6990792192410247	1.1083836712499362
8	0.5950025544914114	1.078138373365347
9	0.5192931031579033	1.0585728489337265
>10	7.0555305848920415	36.219990471942296
>50	1.0155617516886608	16.248473469143562
>100	0.4601250644522753	15.1625619945048
>500	0.005340737355249624	0.849374437770576
>1k	0.003697433553634355	1.1393230298053167
>5k	4.108259504038172E-4	0.6296797568353952
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6767	0.625191128143394	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2140	0.19771080452591444	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1740	0.16075551396032295	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1487	0.13738129267758634	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1426	0.13174561086633363	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1253	0.11576244769671531	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1122	0.1036595900364841	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12028947079100027	0.0
2	0.0	0.0	0.0	0.17073344241303265	0.0
3	0.0	0.0	0.0	0.21572650867664028	0.0
4	0.0	0.0	0.0	0.44919155682476447	0.0
5	0.0	0.0	0.0	0.4959399993902377	0.0
6	0.0	0.0	0.0	0.854591094329303	0.0
7	0.0	0.0	0.0	1.119283363005352	0.0
8	0.0	0.0	0.0	1.397279536285014	0.0
9	0.0	0.0	0.0	1.7287684926583695	0.0
10	0.0	0.0	0.0	2.2534412304633546	0.0
11	0.0	0.0	0.0	2.6761173663073072	0.0
12	0.0	0.0	0.0	2.8798334055501305	0.0
13	0.0	0.0	0.0	2.9743465611716307	0.0
14	0.0	0.0	0.0	3.0918643851702114	0.0
15	0.0	0.0	0.0	3.1857308232068138	0.0
16	0.0	0.0	0.0	3.368936676185734	0.0
17	0.0	0.0	0.0	3.5777340678813254	0.0
18	0.0	0.0	0.0	3.823856303048165	0.0
19	0.0	0.0	0.0	3.9454392090089607	0.0
20	0.0	0.0	0.0	4.081804231195993	0.0
21	0.0	0.0	0.0	4.243853180326112	0.0
22	0.0	0.0	0.0	4.4270590333050315	0.0
23	0.0	0.0	0.0	4.6240307320196345	0.0
24	0.0	0.0	0.0	4.783492810810162	0.0
25	0.0	0.0	0.0	4.922814256242441	0.0
26	0.0	0.0	0.0	5.05049478514656	0.0
27	0.0	0.0	0.0	5.187506524918491	0.0
28	0.0	0.0	0.0	5.332740816841265	0.0
29	0.0	0.0	0.0	5.484349896386604	0.0
30	0.0	0.0	0.0	5.665892761290072	0.0
31	0.0	0.0	0.0	5.8111270532128465	0.0
32	0.0	0.0	0.0	5.982784377890019	0.0
33	0.0	0.0	0.0	6.158691560982235	0.0
34	0.0	0.0	0.0	6.31787647509352	0.0
35	0.0	0.0	0.0	6.496555304978155	0.0
36	0.0	0.0	0.0	6.6696908412779505	0.0
37	0.0	0.0	0.0	6.827767096672268	0.0
38	9.238822641397871E-5	0.0	0.0	7.01263593772664	0.0
39	9.238822641397871E-5	0.0	0.0	7.19630373183763	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	20	7.0331036E-4	45.000004	1
AATTACG	55	1.8189894E-12	45.000004	1
ATACGCG	40	6.8139343E-9	45.000004	1
TAATACG	45	3.8562575E-10	45.0	1
ATTCGCT	25	3.890655E-5	45.0	13
ACGATAG	35	1.2118471E-7	45.0	1
CGCTATA	25	3.890655E-5	45.0	24
TCGACAA	25	3.890655E-5	45.0	14
GTGCGAT	25	3.890655E-5	45.0	33
TAAACGG	170	0.0	42.35294	2
TCGGGAT	185	0.0	41.351353	5
TTACGAG	55	6.184564E-11	40.909092	1
CGAACCG	50	1.0822987E-9	40.5	1
AGATACG	45	1.9281288E-8	40.0	1
CCTCGCG	45	1.9281288E-8	40.0	1
CTTACCG	45	1.9281288E-8	40.0	1
CGCTAAT	40	3.4579716E-7	39.375004	22
GTAACGG	155	0.0	39.193546	2
CATACGA	425	0.0	38.64706	18
TAGCACG	35	6.247952E-6	38.571426	1
>>END_MODULE