FastQCFastQC Report
Sat 18 Jun 2016
SRR3554155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768793
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78581.022121689453468No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16810.218654436239664No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16240.21124021680738506No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12980.1688360846157548No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT11550.1502354990224937Illumina Single End Adapter 2 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA9180.11940795506722875No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7790.10132766557447845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCG302.1645592E-645.0000041
GCGAACG302.1645592E-645.0000041
CGCATAG302.1645592E-645.0000041
TCTTACG302.1645592E-645.0000041
TGACGGT302.1645592E-645.0000049
TCGGACA302.1645592E-645.00000421
AACTGCG302.1645592E-645.0000041
CGTAACG302.1645592E-645.0000041
CTTCGCG351.2112287E-745.01
AACCGGT207.031828E-445.038
CGAACAT207.031828E-445.011
GCGACAG253.889596E-545.01
CATGCGT253.889596E-545.034
ACGCACG453.8380676E-1045.01
TGGACGT207.031828E-445.040
CGGTTTA207.031828E-445.021
CCGGATT253.889596E-545.022
GGATCGA351.2112287E-745.08
CGATTCT253.889596E-545.031
TAACGCG551.8189894E-1245.01