##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768793
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34765144843931	34.0	31.0	34.0	30.0	34.0
2	32.53049650556132	34.0	31.0	34.0	31.0	34.0
3	32.59304260054397	34.0	31.0	34.0	31.0	34.0
4	36.10890447753817	37.0	35.0	37.0	35.0	37.0
5	35.960665614801385	37.0	35.0	37.0	35.0	37.0
6	35.939395910212504	37.0	35.0	37.0	35.0	37.0
7	36.00450316274992	37.0	35.0	37.0	35.0	37.0
8	36.12283800710985	37.0	36.0	37.0	35.0	37.0
9	37.79229259371508	39.0	38.0	39.0	35.0	39.0
10	37.3288999769769	39.0	37.0	39.0	34.0	39.0
11	37.307515807245906	39.0	37.0	39.0	34.0	39.0
12	37.33561179667349	39.0	37.0	39.0	34.0	39.0
13	37.331543081167496	39.0	37.0	39.0	34.0	39.0
14	38.60812078153677	40.0	38.0	41.0	34.0	41.0
15	38.60449431771621	40.0	38.0	41.0	34.0	41.0
16	38.6052474463217	40.0	38.0	41.0	34.0	41.0
17	38.54655674544383	40.0	38.0	41.0	34.0	41.0
18	38.47232349930346	40.0	38.0	41.0	34.0	41.0
19	38.43843271205643	40.0	38.0	41.0	34.0	41.0
20	38.37393680743711	40.0	38.0	41.0	34.0	41.0
21	38.328312042383324	40.0	38.0	41.0	34.0	41.0
22	38.28720084600146	40.0	38.0	41.0	34.0	41.0
23	38.17459446170816	40.0	37.0	41.0	34.0	41.0
24	38.14979454807731	40.0	37.0	41.0	34.0	41.0
25	38.111563190611776	40.0	37.0	41.0	33.0	41.0
26	38.047319629601205	40.0	37.0	41.0	33.0	41.0
27	37.98278210129384	40.0	37.0	41.0	33.0	41.0
28	37.86968013496481	40.0	37.0	41.0	33.0	41.0
29	37.79208967823588	40.0	37.0	41.0	33.0	41.0
30	37.749758387498325	40.0	37.0	41.0	33.0	41.0
31	37.67036250330063	40.0	36.0	41.0	33.0	41.0
32	37.598348320028926	40.0	36.0	41.0	33.0	41.0
33	37.519036983947565	40.0	36.0	41.0	33.0	41.0
34	37.42599243229322	40.0	36.0	41.0	33.0	41.0
35	37.36840475914843	40.0	36.0	41.0	32.0	41.0
36	37.31362278272565	40.0	36.0	41.0	32.0	41.0
37	37.25839985535768	39.0	35.0	41.0	32.0	41.0
38	37.18904048293884	39.0	35.0	41.0	32.0	41.0
39	37.12387859931087	39.0	35.0	41.0	32.0	41.0
40	37.02514981275844	39.0	35.0	41.0	31.0	41.0
41	36.93744479983559	39.0	35.0	41.0	31.0	41.0
42	36.862860353827365	39.0	35.0	41.0	31.0	41.0
43	36.79886263272429	39.0	35.0	41.0	31.0	41.0
44	36.730567265830985	39.0	35.0	41.0	31.0	41.0
45	36.57620842021194	39.0	35.0	41.0	31.0	41.0
46	36.41378758651548	39.0	35.0	40.0	30.0	41.0
47	36.30703843557369	39.0	35.0	40.0	30.0	41.0
48	36.240527684305135	38.0	35.0	40.0	30.0	41.0
49	36.09847514220343	38.0	35.0	40.0	30.0	41.0
50	36.00676124782614	38.0	35.0	40.0	30.0	41.0
51	35.06003046333669	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	2.0
12	8.0
13	10.0
14	20.0
15	36.0
16	57.0
17	93.0
18	170.0
19	314.0
20	529.0
21	859.0
22	1280.0
23	1823.0
24	2433.0
25	3118.0
26	4061.0
27	5155.0
28	6469.0
29	8350.0
30	10841.0
31	14209.0
32	18869.0
33	25347.0
34	38699.0
35	55554.0
36	66731.0
37	95707.0
38	159451.0
39	248469.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.163434110352203	32.14116153502958	19.75252115979204	26.942883194826177
2	24.972782010242028	30.248064173321037	19.039585428067113	25.739568388369822
3	20.44243378906936	32.03307002014847	20.623626906072246	26.900869284709927
4	20.89548161858914	32.575999001031484	19.02163521259949	27.506884167779884
5	18.97871078430735	34.23210148895737	17.289569493999036	29.499618232736253
6	19.670574523961587	39.05797789522017	19.128816209304716	22.142631371513527
7	86.6635102036569	6.264885346250551	3.8764660968557205	3.195138353236827
8	87.25872894264126	6.114129551127546	3.4256295257631115	3.2015119804680845
9	79.52231615012103	10.442342737251769	6.294542223979667	3.740798888647529
10	29.172351985514954	43.85003505494977	13.940163346960755	13.037449612574518
11	22.319922267762585	30.62189692153805	30.213724630687324	16.844456180012042
12	25.946646236373123	27.380192067305504	29.606278933340963	17.066882762980413
13	24.055630059066615	28.941340516888157	26.571651927111716	20.431377496933507
14	19.519298432738072	31.226480990331595	27.519631422242398	21.73458915468793
15	18.730269396313442	33.67902673411439	28.290970391249658	19.299733478322516
16	20.36035707921378	31.031630100690304	29.000394124296136	19.60761869579978
17	18.837450393018667	31.87568044974395	29.129427557222815	20.157441600014568
18	20.260070005840326	31.228952396809024	28.446929147377777	20.06404844997288
19	20.555858338980716	33.25849741087653	26.34441260521363	19.84123164492913
20	22.767376914201872	31.15012753758164	27.853791592795456	18.22870395542103
21	21.653162814957994	32.449306900557104	26.375890519294533	19.521639765190372
22	20.483146958934327	31.427315285128763	26.808906948944646	21.28063080699226
23	19.610090102277205	34.61282816050614	26.43546442280302	19.341617314413632
24	18.4041738153183	32.7249337598027	29.129297483197686	19.741594941681313
25	19.177984190802984	34.492249539212764	25.336599058524207	20.993167211460044
26	21.15614996494505	32.11904895075788	26.323730835218324	20.40107024907875
27	18.91939702884912	33.44255215643223	26.4804700354972	21.157580779221455
28	19.6002044763675	33.69905813398405	27.40698731648181	19.29375007316664
29	19.773072855762212	33.25173356156989	25.774948523204554	21.20024505946334
30	20.39534699197313	32.47454126143188	26.939761418223114	20.190350328371874
31	20.023335280107908	33.38284817889861	25.133033209199358	21.460783331794122
32	20.312099615891405	32.49002007042208	26.687027587399992	20.51085272628653
33	19.229233356703297	33.19320025026242	26.277164334222608	21.30040205881167
34	17.687205788814413	32.949961823273625	28.359389328466833	21.003443059445132
35	19.261751862985225	32.19761366193501	27.63084471372658	20.90978976135319
36	19.663810674654947	35.27061250557693	25.009853107403423	20.055723712364706
37	18.702953851036625	34.41888778904074	26.18077948160298	20.697378878319654
38	18.820800917802323	31.84316194346202	29.461896765449218	19.874140373286437
39	18.5356786547224	32.953213673901814	27.077509810833345	21.433597860542434
40	21.32927849239002	31.86319334333169	28.16661962322758	18.640908541050713
41	17.860074168209128	31.885175853578275	29.79644715807766	20.45830282013494
42	19.273848747322102	33.61047772287208	26.540694309131325	20.574979220674486
43	19.234696465758663	33.304543615771735	27.175845773829888	20.284914144639714
44	20.093575253676867	32.2910068119767	26.503493137944805	21.11192479640163
45	19.87739222391463	33.01252742936005	26.959792818092776	20.150287528632546
46	18.094987857589754	33.36125589072741	27.973719843963202	20.570036407719634
47	18.125165031419378	34.15756907255919	28.392948426949776	19.324317469071648
48	19.102931478304303	32.54296019864905	28.45837566158901	19.895732661457636
49	17.89207237839054	34.41602616048793	27.34299089611898	20.34891056500254
50	17.87360186682241	32.95620537647975	28.910643046958022	20.25954970973981
51	17.656118096808893	31.51407465988894	28.50741356906215	22.32239367424001
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9300.0
1	7092.5
2	4885.0
3	3155.5
4	1426.0
5	1480.0
6	1534.0
7	1660.5
8	1787.0
9	2267.5
10	2748.0
11	3346.0
12	3944.0
13	4475.0
14	5006.0
15	5173.0
16	5340.0
17	5358.0
18	5376.0
19	5445.5
20	5515.0
21	5273.0
22	5031.0
23	5980.0
24	6929.0
25	7256.5
26	10065.5
27	12547.0
28	14856.0
29	17165.0
30	19214.0
31	21263.0
32	27379.0
33	33495.0
34	36825.5
35	40156.0
36	41713.5
37	43271.0
38	43244.5
39	43218.0
40	42603.5
41	41989.0
42	41824.0
43	41659.0
44	42597.0
45	43535.0
46	45569.5
47	47604.0
48	49796.5
49	51989.0
50	53411.5
51	54834.0
52	51701.0
53	48568.0
54	43373.0
55	38178.0
56	33150.5
57	28123.0
58	25919.5
59	23716.0
60	23021.0
61	22326.0
62	19890.5
63	17455.0
64	14516.5
65	11578.0
66	9380.5
67	7183.0
68	5626.5
69	4070.0
70	3553.5
71	3037.0
72	2456.5
73	1876.0
74	1783.5
75	1285.5
76	880.0
77	620.0
78	360.0
79	338.5
80	317.0
81	219.5
82	122.0
83	96.5
84	71.0
85	53.5
86	36.0
87	45.5
88	55.0
89	32.0
90	9.0
91	7.0
92	5.0
93	2.5
94	0.0
95	2.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	768793.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.61345318516524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0850378622051	17.02176666910044
2	9.393890535030451	4.436443887510168
3	3.9283425081956653	2.782851957377188
4	2.3826037025765117	2.2504600395836727
5	1.5877141523766454	1.8745706904285113
6	1.1790112961570212	1.6704316827950885
7	0.852867525092541	1.409740316384432
8	0.7203028660082108	1.360707040450019
9	0.5612209641025253	1.1927128466131454
>10	5.8258774247089775	30.489938587239774
>50	1.004808906993927	16.614396207062175
>100	0.47278611932385717	16.642502580276023
>500	0.002768068614308297	0.4734943431685591
>1k	0.0022144548914466377	0.75272789286708
>5k	5.536137228616594E-4	1.0272552591437156
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7858	1.022121689453468	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1681	0.218654436239664	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1624	0.21124021680738506	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1298	0.1688360846157548	No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1155	0.1502354990224937	Illumina Single End Adapter 2 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	918	0.11940795506722875	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	779	0.10132766557447845	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6014805025540035E-4	0.0	0.0	0.17586008197265063	0.0
2	2.6014805025540035E-4	0.0	0.0	0.2649607891851253	0.0
3	2.6014805025540035E-4	0.0	0.0	0.34144431596021296	0.0
4	2.6014805025540035E-4	0.0	0.0	0.7276340965643547	0.0
5	2.6014805025540035E-4	0.0	0.0	0.7993048844097176	0.0
6	2.6014805025540035E-4	0.0	0.0	1.2782374449299096	0.0
7	2.6014805025540035E-4	0.0	0.0	1.6100562830306728	0.0
8	2.6014805025540035E-4	0.0	0.0	1.9743936274133609	0.0
9	2.6014805025540035E-4	0.0	0.0	2.3989552454301744	0.0
10	2.6014805025540035E-4	0.0	0.0	3.111760903129971	0.0
11	2.6014805025540035E-4	0.0	0.0	3.663795065771931	0.0
12	2.6014805025540035E-4	0.0	0.0	3.9312272614344823	0.0
13	2.6014805025540035E-4	0.0	0.0	4.055057733356053	0.0
14	2.6014805025540035E-4	0.0	0.0	4.191635459740138	0.0
15	2.6014805025540035E-4	0.0	0.0	4.310913340782239	0.0
16	2.6014805025540035E-4	0.0	0.0	4.53711207047931	0.0
17	2.6014805025540035E-4	0.0	0.0	4.821844111483846	0.0
18	2.6014805025540035E-4	0.0	0.0	5.161467391092271	0.0
19	2.6014805025540035E-4	0.0	0.0	5.314304370617318	0.0
20	2.6014805025540035E-4	0.0	0.0	5.494456895419183	0.0
21	2.6014805025540035E-4	0.0	0.0	5.687616822733818	0.0
22	2.6014805025540035E-4	0.0	0.0	5.93579806267747	0.0
23	2.6014805025540035E-4	0.0	0.0	6.17331323256065	0.0
24	2.6014805025540035E-4	0.0	0.0	6.3519048690609825	0.0
25	2.6014805025540035E-4	0.0	0.0	6.506302736887562	0.0
26	2.6014805025540035E-4	0.0	0.0	6.655497643709035	0.0
27	2.6014805025540035E-4	0.0	0.0	6.8264149127268325	0.0
28	2.6014805025540035E-4	0.0	0.0	6.985235297407755	0.0
29	2.6014805025540035E-4	0.0	0.0	7.163566785857832	0.0
30	2.6014805025540035E-4	0.0	0.0	7.417341208881974	0.0
31	2.6014805025540035E-4	0.0	0.0	7.594241883055647	0.0
32	2.6014805025540035E-4	0.0	0.0	7.787922106470792	0.0
33	2.6014805025540035E-4	0.0	0.0	7.980171515609533	0.0
34	2.6014805025540035E-4	0.0	0.0	8.167217963743166	0.0
35	2.6014805025540035E-4	0.0	0.0	8.384571659731554	0.0
36	2.6014805025540035E-4	0.0	0.0	8.585275880503595	0.0
37	2.6014805025540035E-4	0.0	0.0	8.785329731149998	0.0
38	2.6014805025540035E-4	0.0	0.0	8.99396846745483	0.0
39	2.6014805025540035E-4	0.0	0.0	9.206379350488364	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACCG	30	2.1645592E-6	45.000004	1
GCGAACG	30	2.1645592E-6	45.000004	1
CGCATAG	30	2.1645592E-6	45.000004	1
TCTTACG	30	2.1645592E-6	45.000004	1
TGACGGT	30	2.1645592E-6	45.000004	9
TCGGACA	30	2.1645592E-6	45.000004	21
AACTGCG	30	2.1645592E-6	45.000004	1
CGTAACG	30	2.1645592E-6	45.000004	1
CTTCGCG	35	1.2112287E-7	45.0	1
AACCGGT	20	7.031828E-4	45.0	38
CGAACAT	20	7.031828E-4	45.0	11
GCGACAG	25	3.889596E-5	45.0	1
CATGCGT	25	3.889596E-5	45.0	34
ACGCACG	45	3.8380676E-10	45.0	1
TGGACGT	20	7.031828E-4	45.0	40
CGGTTTA	20	7.031828E-4	45.0	21
CCGGATT	25	3.889596E-5	45.0	22
GGATCGA	35	1.2112287E-7	45.0	8
CGATTCT	25	3.889596E-5	45.0	31
TAACGCG	55	1.8189894E-12	45.0	1
>>END_MODULE