FastQCFastQC Report
Sat 18 Jun 2016
SRR3554147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105630
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56150.5078552499479935No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14840.13422211770664688No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14580.13187051726165172No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT12120.10962075920515905No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG253.8907092E-545.01
ACCTACG207.0331694E-445.01
TCATACG253.8907092E-545.01
CCGGCAT302.1654268E-644.9999965
TCTAGCG600.044.9999961
TATCGCG302.1654268E-644.9999961
ATACCGG2050.042.804882
TAACGGG5850.041.9230733
ATAGCGG2100.041.7857132
AACGTTG650.041.538461
AACTACG556.184564E-1140.9090921
ATAAGCG1050.040.7142871
TATACCG1050.040.7142871
TATAGCG501.0822987E-940.51
CGATAGG950.040.2631572
AACGGGA11850.040.2531624
CGAATAT2750.040.09090813
ACTCACG900.040.01
AAGGGAT13700.039.416065
ACGAATA2800.039.37500412
Produced by FastQC (version 0.11.5)