##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1105630
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39774336803451	34.0	31.0	34.0	30.0	34.0
2	32.55886055913823	34.0	31.0	34.0	31.0	34.0
3	32.65426046688314	34.0	31.0	34.0	31.0	34.0
4	36.14818971988821	37.0	35.0	37.0	35.0	37.0
5	36.001286144551074	37.0	35.0	37.0	35.0	37.0
6	35.98268679395458	37.0	35.0	37.0	35.0	37.0
7	36.01354431410146	37.0	35.0	37.0	35.0	37.0
8	36.11957345585775	37.0	36.0	37.0	35.0	37.0
9	37.747633475936794	39.0	38.0	39.0	35.0	39.0
10	37.37599920407369	39.0	37.0	39.0	35.0	39.0
11	37.32731564809204	39.0	37.0	39.0	34.0	39.0
12	37.30054267702577	39.0	37.0	39.0	34.0	39.0
13	37.28563895697476	39.0	37.0	39.0	34.0	39.0
14	38.54991905067699	40.0	38.0	41.0	34.0	41.0
15	38.55164476361893	40.0	38.0	41.0	34.0	41.0
16	38.555683185152354	40.0	38.0	41.0	34.0	41.0
17	38.508879100603274	40.0	38.0	41.0	34.0	41.0
18	38.441194613026056	40.0	38.0	41.0	34.0	41.0
19	38.437454663856805	40.0	38.0	41.0	34.0	41.0
20	38.34978880819081	40.0	38.0	41.0	34.0	41.0
21	38.31045015059288	40.0	38.0	41.0	34.0	41.0
22	38.24988558559373	40.0	37.0	41.0	34.0	41.0
23	38.18070692727223	40.0	37.0	41.0	34.0	41.0
24	38.12976221701654	40.0	37.0	41.0	34.0	41.0
25	38.0435597803967	40.0	37.0	41.0	33.0	41.0
26	37.97145157059776	40.0	37.0	41.0	33.0	41.0
27	37.90372185993506	40.0	37.0	41.0	33.0	41.0
28	37.787871168474084	40.0	37.0	41.0	33.0	41.0
29	37.70149417074428	40.0	36.0	41.0	33.0	41.0
30	37.648668180132596	40.0	36.0	41.0	33.0	41.0
31	37.56119678373416	40.0	36.0	41.0	33.0	41.0
32	37.51706176569015	40.0	36.0	41.0	33.0	41.0
33	37.45015240179807	40.0	36.0	41.0	33.0	41.0
34	37.335997576042615	40.0	36.0	41.0	32.0	41.0
35	37.2762913452059	40.0	36.0	41.0	32.0	41.0
36	37.18501940070367	39.0	35.0	41.0	32.0	41.0
37	37.09734540488228	39.0	35.0	41.0	31.0	41.0
38	37.02868952542894	39.0	35.0	41.0	31.0	41.0
39	36.956486347150495	39.0	35.0	41.0	31.0	41.0
40	36.83716161826289	39.0	35.0	41.0	31.0	41.0
41	36.74791114568165	39.0	35.0	41.0	31.0	41.0
42	36.65155522190968	39.0	35.0	41.0	31.0	41.0
43	36.56480739487894	39.0	35.0	41.0	30.0	41.0
44	36.46962817579118	39.0	35.0	41.0	30.0	41.0
45	36.34571059034216	39.0	35.0	40.0	30.0	41.0
46	36.21380299015041	38.0	35.0	40.0	30.0	41.0
47	36.070028852328534	38.0	35.0	40.0	30.0	41.0
48	35.96739596429185	38.0	35.0	40.0	29.0	41.0
49	35.86035201649738	38.0	35.0	40.0	29.0	41.0
50	35.75967547913859	38.0	34.0	40.0	29.0	41.0
51	34.73010229461936	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	5.0
10	9.0
11	12.0
12	15.0
13	17.0
14	28.0
15	49.0
16	91.0
17	170.0
18	306.0
19	532.0
20	875.0
21	1374.0
22	1985.0
23	2928.0
24	3932.0
25	5133.0
26	6421.0
27	8070.0
28	10304.0
29	12793.0
30	16744.0
31	21518.0
32	28030.0
33	37796.0
34	60265.0
35	77947.0
36	92319.0
37	134413.0
38	227290.0
39	354090.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.575183379611623	32.1439360364679	20.128071778081274	25.152808805839204
2	23.4696960104194	30.828486926005986	19.764206832303756	25.93761023127086
3	21.842026717798902	30.338992248763148	20.885287121369718	26.933693912068236
4	22.15686983891537	31.131300706384597	19.08848348905149	27.623345965648543
5	19.66173132060454	33.53083762198927	16.83800186319112	29.969429194215063
6	21.21523475303673	37.0784077856064	18.748315440065845	22.95804202129103
7	85.1670993008511	6.12293443557067	4.795636876712824	3.9143293868654068
8	86.3407288152456	5.740618470917034	4.512992592458598	3.405660121378761
9	77.37968398107866	10.46543599576712	7.2271917368378205	4.927688286316399
10	34.532076734531444	36.53618299069309	14.123802718811898	14.80793755596357
11	25.244521223194017	29.303021806571817	27.159809339471614	18.292647630762552
12	26.951150023063775	24.736304188562176	29.58032976673933	18.732216021634724
13	24.47771858578367	26.29098342121686	28.21775820120655	21.013539791792915
14	21.602706149435164	27.92661197688196	28.846087750875064	21.62459412280781
15	21.275019672042184	29.427656630156562	28.88036684966942	20.416956848131836
16	22.731836147716685	27.5855394661867	28.96873275869866	20.713891627397953
17	21.847905718911388	27.82368423432794	28.743793131517776	21.584616915242893
18	22.406229932255815	28.038584336532114	28.44233604370359	21.11284968750848
19	22.440056800195364	28.605862720801717	27.71532972151624	21.23875075748668
20	22.955509528504113	28.02845436538444	28.250771053607444	20.765265052504002
21	23.679621573220697	28.069155142317047	27.480621907871534	20.770601376590722
22	22.559355299693387	27.533713810225848	27.776742671598996	22.13018821848177
23	22.427213443918852	28.36220073623183	27.303166520445355	21.90741929940396
24	21.24046923473495	28.324575129111906	28.99568571764514	21.439269918508
25	21.625769923030308	30.071995152085236	26.27551712598247	22.026717798901984
26	22.557908160958007	28.4149308539023	26.817741920895777	22.209419064243914
27	21.80114504852437	27.788500673823975	27.730253339724865	22.68010093792679
28	21.63273427819433	29.199732279333958	28.599260150321538	20.568273292150177
29	21.86120130604271	29.04705914275119	27.003699248392323	22.08804030281378
30	22.20580121740546	28.495427946057905	27.996526867035083	21.302243969501554
31	22.57165597894413	28.3559599504355	27.20304260919114	21.869341461429233
32	22.87790671381927	28.471188372240263	27.877318813708023	20.773586100232446
33	21.977062851044202	29.117335817588163	26.775684451398753	22.129916879968885
34	20.866926548664562	28.959055018405795	29.183723307073794	20.990295125855845
35	23.1139712200284	27.325325832330886	28.85504192180024	20.70566102584047
36	23.192930727277663	30.069733997811205	26.98769027613216	19.749644998778976
37	21.47662418711504	29.908649367329033	27.7006774418205	20.91404900373543
38	21.442345088320685	28.272930365492975	29.35891754022594	20.925807005960404
39	22.492425133181985	27.779727395240723	27.661966480649042	22.06588099092825
40	22.195580800086827	28.739089930627788	28.77400215261887	20.291327116666515
41	21.252408129301845	27.50404746615052	30.696073731718563	20.547470672829064
42	21.129401336794405	28.969004097211542	28.10849922668524	21.79309533930881
43	21.269864240297387	28.088781961415666	29.090021073957832	21.551332724329114
44	21.386539800837532	27.782440780369562	29.136239067319085	21.69478035147382
45	21.652180204951023	28.54345486283838	28.39747474290676	21.406890189303837
46	20.57903638649458	28.332263053643626	29.48147210187857	21.607228457983233
47	21.655526713276593	28.939156860794295	28.430397149136695	20.974919276792416
48	20.87162974955455	28.190986134601992	29.23952859455695	21.697855521286506
49	20.537973824878122	29.964273762470267	28.425784394417665	21.071968018233946
50	20.81935186273889	28.117091612926565	29.39138771560106	21.67216880873348
51	21.440355272559536	27.555692229769452	28.954623156028692	22.04932934164232
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7078.0
1	5506.0
2	3934.0
3	2794.0
4	1654.0
5	1608.0
6	1562.0
7	1822.5
8	2083.0
9	2263.0
10	2443.0
11	2852.5
12	3262.0
13	3529.5
14	3797.0
15	3885.0
16	3973.0
17	3976.0
18	3979.0
19	4419.5
20	4860.0
21	5077.5
22	5295.0
23	6679.5
24	8064.0
25	9053.5
26	12397.5
27	14752.0
28	17313.0
29	19874.0
30	25966.0
31	32058.0
32	34341.5
33	36625.0
34	40742.5
35	44860.0
36	47175.0
37	49490.0
38	52877.5
39	56265.0
40	58756.5
41	61248.0
42	62836.5
43	64425.0
44	64607.0
45	64789.0
46	68939.0
47	73089.0
48	76817.0
49	80545.0
50	75763.5
51	70982.0
52	66968.5
53	62955.0
54	58635.0
55	54315.0
56	49944.5
57	45574.0
58	44247.5
59	42921.0
60	42058.5
61	41196.0
62	38323.0
63	35450.0
64	30434.0
65	25418.0
66	22186.5
67	18955.0
68	16937.0
69	14919.0
70	13075.5
71	11232.0
72	9513.5
73	7795.0
74	6698.5
75	4642.5
76	3683.0
77	2895.0
78	2107.0
79	1660.5
80	1214.0
81	920.0
82	626.0
83	468.0
84	310.0
85	258.0
86	206.0
87	128.0
88	50.0
89	37.5
90	25.0
91	19.0
92	13.0
93	12.0
94	11.0
95	11.0
96	11.0
97	10.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1105630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.183862864094035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.98609146880801	16.191134441302605
2	8.406874666309388	3.729939094260675
3	3.258585021457184	2.168640097409912
4	1.8316643976443638	1.6253356724154386
5	1.3113658988266785	1.4545580632110224
6	1.0250904616566576	1.364427973480928
7	0.7998757322625307	1.2421033486980144
8	0.6644708554176398	1.179242426701774
9	0.5840677874791385	1.1661191730694824
>10	7.614380818854081	37.9422274579799
>50	1.1027651847727178	16.97507506423275
>100	0.4073831182651332	13.063448639586953
>500	0.004512803928415372	0.7210752802146291
>1k	0.0024615294154992935	0.6656499557604186
>5k	4.102549025832156E-4	0.5110233116755196
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5615	0.5078552499479935	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1484	0.13422211770664688	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1458	0.13187051726165172	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	1212	0.10962075920515905	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.808923419227047E-4	0.0	0.0	0.09523981802230402	0.0
2	1.808923419227047E-4	0.0	0.0	0.14815082803469515	0.0
3	1.808923419227047E-4	0.0	0.0	0.20486057722746306	0.0
4	1.808923419227047E-4	0.0	0.0	0.43812125213679076	0.0
5	1.808923419227047E-4	0.0	0.0	0.49763483262936065	0.0
6	1.808923419227047E-4	0.0	0.0	0.8835686441214511	0.0
7	1.808923419227047E-4	0.0	0.0	1.1322051680942087	0.0
8	1.808923419227047E-4	0.0	0.0	1.3898863091631015	0.0
9	1.808923419227047E-4	0.0	0.0	1.6874542116259508	0.0
10	1.808923419227047E-4	0.0	0.0	2.2304930220779102	0.0
11	1.808923419227047E-4	0.0	0.0	2.645007823593788	0.0
12	1.808923419227047E-4	0.0	0.0	2.853938478514512	0.0
13	1.808923419227047E-4	0.0	0.0	2.9539719435977676	0.0
14	1.808923419227047E-4	0.0	0.0	3.0682054575219557	0.0
15	1.808923419227047E-4	0.0	0.0	3.157294935918888	0.0
16	1.808923419227047E-4	0.0	0.0	3.315213950417409	0.0
17	1.808923419227047E-4	0.0	0.0	3.498005661930302	0.0
18	1.808923419227047E-4	0.0	0.0	3.719779673127538	0.0
19	1.808923419227047E-4	0.0	0.0	3.8394399573094073	0.0
20	1.808923419227047E-4	0.0	0.0	3.9735716288450926	0.0
21	1.808923419227047E-4	0.0	0.0	4.115300778741532	0.0
22	1.808923419227047E-4	0.0	0.0	4.277832547959082	0.0
23	1.808923419227047E-4	0.0	0.0	4.445067518066622	0.0
24	1.808923419227047E-4	0.0	0.0	4.5763953583025065	0.0
25	1.808923419227047E-4	0.0	0.0	4.701120628058211	0.0
26	1.808923419227047E-4	0.0	0.0	4.821866266291616	0.0
27	1.808923419227047E-4	0.0	0.0	4.95183741396308	0.0
28	1.808923419227047E-4	0.0	0.0	5.086421316353572	0.0
29	1.808923419227047E-4	0.0	0.0	5.2230854806761755	0.0
30	1.808923419227047E-4	0.0	0.0	5.402892468547344	0.0
31	2.7133851288405704E-4	0.0	0.0	5.546520988033971	0.0
32	2.7133851288405704E-4	0.0	0.0	5.7038068793357635	0.0
33	2.7133851288405704E-4	0.0	0.0	5.854851984841222	0.0
34	2.7133851288405704E-4	0.0	0.0	5.9984805043278495	0.0
35	2.7133851288405704E-4	0.0	0.0	6.167524397854617	0.0
36	2.7133851288405704E-4	0.0	0.0	6.330779736439858	0.0
37	2.7133851288405704E-4	0.0	0.0	6.4892414279641475	0.0
38	2.7133851288405704E-4	0.0	0.0	6.650054719933432	0.0
39	2.7133851288405704E-4	0.0	0.0	6.81982218282789	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCG	25	3.8907092E-5	45.0	1
ACCTACG	20	7.0331694E-4	45.0	1
TCATACG	25	3.8907092E-5	45.0	1
CCGGCAT	30	2.1654268E-6	44.999996	5
TCTAGCG	60	0.0	44.999996	1
TATCGCG	30	2.1654268E-6	44.999996	1
ATACCGG	205	0.0	42.80488	2
TAACGGG	585	0.0	41.923073	3
ATAGCGG	210	0.0	41.785713	2
AACGTTG	65	0.0	41.53846	1
AACTACG	55	6.184564E-11	40.909092	1
ATAAGCG	105	0.0	40.714287	1
TATACCG	105	0.0	40.714287	1
TATAGCG	50	1.0822987E-9	40.5	1
CGATAGG	95	0.0	40.263157	2
AACGGGA	1185	0.0	40.253162	4
CGAATAT	275	0.0	40.090908	13
ACTCACG	90	0.0	40.0	1
AAGGGAT	1370	0.0	39.41606	5
ACGAATA	280	0.0	39.375004	12
>>END_MODULE