FastQCFastQC Report
Sat 18 Jun 2016
SRR3554141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213477
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34870.2873560850349862No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21360.1760231137466965No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA18450.15204243673345272No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16890.13918681606655917No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA15500.12773212842105783No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT13220.10894314436944418No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT13060.10762461917283969TruSeq Adapter, Index 23 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG551.8189894E-1245.01
ATTACGC207.0334435E-445.01
ATATGCG253.8909355E-545.01
GCGATCG207.0334435E-445.09
GTATACG207.0334435E-445.01
TACGTAA207.0334435E-445.045
TATCGCG600.044.9999961
CGATAAG302.1656033E-644.9999961
TAACGCG700.041.7857131
CGTATAG603.6379788E-1241.2499961
CGCATAG603.6379788E-1241.2499961
TATACGG1200.041.2499962
TTACGTA556.184564E-1140.90909244
CCGAACG501.0822987E-940.51
CGGGTTA950.040.2631576
TCATACG451.9284926E-840.01
CTAACGG2500.039.62
AAACGCG403.4584264E-739.3751
TAAGACG403.4584264E-739.3751
ATACCGG1550.039.1935462