SRR3554140_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	988985
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5870	0.5935378190771347	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2271	0.22962936748282328	RNA PCR Primer, Index 16 (96% over 26bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1675	0.16936556166170366	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1398	0.14135704788242492	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1284	0.12983007831261342	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	1275	0.12892005439920726	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1141	0.11537080946627098	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1116	0.11284296526236495	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1050	0.10616945656405305	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCCC	25	3.8904098E-5	45.0	12
CGAACCG	20	7.032809E-4	45.0	1
TCACACG	55	1.8189894E-12	45.0	1
ACGATAG	40	6.8139343E-9	45.0	1
TATTACG	20	7.032809E-4	45.0	1
GTATACG	45	3.8380676E-10	45.0	1
TATAGCG	20	7.032809E-4	45.0	1
CGCAATG	20	7.032809E-4	45.0	1
CGCGTAG	20	7.032809E-4	45.0	1
TCGAACG	25	3.8904098E-5	45.0	1
TACCGAC	20	7.032809E-4	45.0	20
AATTGCG	60	0.0	44.999996	1
ACGTAGA	30	2.165194E-6	44.999996	38
CGTAACG	75	0.0	42.0	1
AACGGGA	775	0.0	41.51613	4
TCTCGCG	60	3.6379788E-12	41.249996	1
TATCGCG	50	1.0804797E-9	40.5	1
GTAGGGA	390	0.0	40.384617	4
CATACGA	465	0.0	40.161293	18
ATTCACG	45	1.927765E-8	40.0	1