##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	988985
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18813126589382	33.0	31.0	34.0	30.0	34.0
2	32.38341228633397	34.0	31.0	34.0	30.0	34.0
3	32.46704955080209	34.0	31.0	34.0	30.0	34.0
4	36.018953775840885	37.0	35.0	37.0	35.0	37.0
5	35.82763742625014	37.0	35.0	37.0	35.0	37.0
6	35.79734980813662	37.0	35.0	37.0	35.0	37.0
7	35.832731537889856	37.0	35.0	37.0	35.0	37.0
8	35.96518855189917	37.0	35.0	37.0	35.0	37.0
9	37.55718438601192	39.0	37.0	39.0	35.0	39.0
10	37.190600464112194	39.0	37.0	39.0	34.0	39.0
11	37.16183966389784	39.0	37.0	39.0	34.0	39.0
12	37.078921318321306	39.0	37.0	39.0	33.0	39.0
13	37.04943249897622	39.0	37.0	39.0	33.0	39.0
14	38.26622446245393	40.0	38.0	41.0	33.0	41.0
15	38.2669828157151	40.0	38.0	41.0	33.0	41.0
16	38.28541282223694	40.0	38.0	41.0	33.0	41.0
17	38.247448646845	40.0	37.0	41.0	33.0	41.0
18	38.17299251252547	40.0	37.0	41.0	33.0	41.0
19	38.138633042968294	40.0	37.0	41.0	34.0	41.0
20	38.05544270135543	40.0	37.0	41.0	33.0	41.0
21	38.022887101422164	40.0	37.0	41.0	33.0	41.0
22	37.9528314382928	40.0	37.0	41.0	33.0	41.0
23	37.87675849482045	40.0	37.0	41.0	33.0	41.0
24	37.79656920984646	40.0	37.0	41.0	33.0	41.0
25	37.73201615798015	40.0	36.0	41.0	33.0	41.0
26	37.62776179618498	40.0	36.0	41.0	33.0	41.0
27	37.54394252693418	40.0	36.0	41.0	33.0	41.0
28	37.45464289144932	40.0	36.0	41.0	32.0	41.0
29	37.31693503945965	39.0	36.0	41.0	32.0	41.0
30	37.28295777994611	39.0	36.0	41.0	32.0	41.0
31	37.237330192065606	39.0	36.0	41.0	32.0	41.0
32	37.166408995080815	39.0	35.0	41.0	31.0	41.0
33	37.04465992911925	39.0	35.0	41.0	31.0	41.0
34	36.971520296061115	39.0	35.0	41.0	31.0	41.0
35	36.915280818212615	39.0	35.0	41.0	31.0	41.0
36	36.8008776675076	39.0	35.0	41.0	31.0	41.0
37	36.73896874067857	39.0	35.0	41.0	31.0	41.0
38	36.650416335940385	39.0	35.0	41.0	30.0	41.0
39	36.578738807969785	39.0	35.0	41.0	30.0	41.0
40	36.47130745157914	39.0	35.0	41.0	30.0	41.0
41	36.36967598092994	39.0	35.0	40.0	30.0	41.0
42	36.270031395825015	39.0	35.0	40.0	30.0	41.0
43	36.163180432463584	38.0	35.0	40.0	30.0	41.0
44	36.06388165644575	38.0	35.0	40.0	29.0	41.0
45	35.89475371213921	38.0	35.0	40.0	29.0	41.0
46	35.796432706259445	38.0	35.0	40.0	29.0	41.0
47	35.63839390890661	38.0	34.0	40.0	28.0	41.0
48	35.55642401047539	38.0	34.0	40.0	28.0	41.0
49	35.3882970924736	38.0	34.0	40.0	27.0	41.0
50	35.26818708069384	38.0	34.0	40.0	27.0	41.0
51	34.15011350020475	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	8.0
12	11.0
13	15.0
14	31.0
15	52.0
16	79.0
17	170.0
18	333.0
19	569.0
20	939.0
21	1500.0
22	2129.0
23	3023.0
24	4065.0
25	5331.0
26	6690.0
27	8576.0
28	10750.0
29	13773.0
30	17624.0
31	22802.0
32	29276.0
33	39696.0
34	59883.0
35	76459.0
36	89274.0
37	126765.0
38	196523.0
39	272507.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.20526094935717	32.84670647178673	19.032240124976617	27.915792453879483
2	24.276707937936372	30.992987760178366	19.518395122271826	25.21190917961344
3	21.04551636273553	31.510488025602008	20.52265706759961	26.921338544062852
4	22.44159415966875	31.979049227238026	18.296637461639964	27.282719151453254
5	19.16267688589817	33.89242506205857	17.123818864795727	29.82107918724753
6	20.700819527090903	39.120916899649636	18.76883875892961	21.409424814329846
7	82.2665662269903	7.559467534896889	5.534967668872632	4.63899856924018
8	83.72260448844017	6.36834734601637	5.4179790391158615	4.4910691264276
9	74.01386269761421	11.365895337138582	9.121270797838188	5.49897116740901
10	33.67189593370981	33.494845725668235	15.56868911055274	17.264569230069213
11	24.78379348523992	29.167479789885594	26.55692452362776	19.491802201246735
12	26.78938507661896	24.192783510366688	29.797519679267126	19.220311733747224
13	24.21381517414319	26.536297314923885	27.68899427190503	21.56089323902789
14	20.682517935054626	27.94713772200792	28.79356107524381	22.576783267693646
15	19.129006001102137	30.367801331668325	28.69730076795907	21.805891899270463
16	20.740658351744464	27.447130138475305	28.575863132403423	23.236348377376807
17	20.395354833490902	27.906894442281732	29.053423459405348	22.644327264822014
18	21.319332446902635	27.93065617779845	27.69182545741341	23.05818591788551
19	21.41791837085497	29.68477782777292	27.572308983452732	21.324994817919382
20	22.161812363180434	28.19688872935383	27.973123960424072	21.668174947041663
21	22.370207839350446	28.797605625970057	27.366138010182155	21.46604852449734
22	21.313872303422198	27.837227056022083	26.956728362917538	23.892172277638185
23	20.83904204816049	29.964559624261238	26.482707017801076	22.713691309777197
24	19.76632608179093	28.423585797560126	29.4195564138991	22.39053170674985
25	21.177469830179426	29.99994944311592	25.88138343857591	22.941197288128738
26	22.429763848794472	27.783838986435587	26.520928022164135	23.265469142605802
27	19.922749081128632	28.583648892551455	27.678175098712316	23.815426927607596
28	21.14946131640015	28.681931475199317	28.085764698150122	22.08284251025041
29	20.214260074723075	29.182141286268244	26.368650687320837	24.23494795168784
30	21.27544907152282	29.034515184760135	27.936217435047045	21.75381830867
31	22.040981410233723	28.855847156428055	25.148106391906854	23.955065041431368
32	21.162707220028615	28.447246419308687	26.972097655677285	23.417948704985413
33	20.429935742200335	29.729065658225352	26.584933037407037	23.256065562167272
34	19.966531342740286	28.01589508435416	27.887480598795733	24.13009297410982
35	21.448353615069994	27.9873810017341	28.092640434384748	22.471624948811154
36	21.50770739697771	30.37932830123814	27.29363943841413	20.81932486337002
37	19.44700880195352	30.4152236889336	26.895453419414856	23.242314089698024
38	19.126174815593767	28.897303801372114	28.836534426710216	23.139986956323906
39	19.89453833981304	28.41377776204897	27.370789243517347	24.320894654620645
40	21.094657654059464	29.0283472449026	27.010015318735874	22.866979782302057
41	19.492206656319357	27.390405314539656	29.840695258269847	23.276692770871147
42	20.376952127686465	28.090618159021624	27.304863066679474	24.227566646612438
43	20.014257041310028	28.56787514471908	28.131164780052277	23.286703033918613
44	21.278381370799355	26.682912278750436	27.203445957218765	24.835260393231444
45	21.121655030157182	28.937850422402768	27.255924002891852	22.6845705445482
46	18.992805755395683	28.692649534623882	29.327846226181386	22.986698483799046
47	19.77623523107024	28.911560842682142	28.130861438747807	23.18134248749981
48	19.40312542657371	27.7054758161145	29.17981566960065	23.711583087711137
49	19.151756598937293	29.21854224280449	28.492545387442682	23.137155770815532
50	19.878764591980666	26.23811281263113	29.704495012563388	24.178627582824816
51	19.79908694267355	26.264907961192534	29.06363594998913	24.872369146144784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7380.0
1	5713.5
2	4047.0
3	2747.0
4	1447.0
5	1425.5
6	1404.0
7	1494.0
8	1584.0
9	1743.5
10	1903.0
11	2132.0
12	2361.0
13	2625.0
14	2889.0
15	3144.0
16	3399.0
17	3486.0
18	3573.0
19	3436.5
20	3300.0
21	3612.5
22	3925.0
23	5064.5
24	6204.0
25	7061.5
26	9505.0
27	11091.0
28	14482.5
29	17874.0
30	20921.5
31	23969.0
32	28742.0
33	33515.0
34	37126.0
35	40737.0
36	43537.5
37	46338.0
38	47435.0
39	48532.0
40	49800.0
41	51068.0
42	52176.0
43	53284.0
44	54203.0
45	55122.0
46	60489.0
47	65856.0
48	67706.0
49	69556.0
50	72830.5
51	76105.0
52	71160.5
53	66216.0
54	61010.5
55	55805.0
56	50636.5
57	45468.0
58	41644.5
59	37821.0
60	36231.0
61	34641.0
62	32045.0
63	29449.0
64	25845.0
65	22241.0
66	18808.0
67	15375.0
68	13752.5
69	12130.0
70	10410.0
71	8690.0
72	7590.0
73	6490.0
74	5346.0
75	3535.0
76	2868.0
77	2243.5
78	1619.0
79	1189.0
80	759.0
81	568.0
82	377.0
83	302.5
84	228.0
85	163.5
86	99.0
87	75.0
88	51.0
89	47.0
90	43.0
91	28.0
92	13.0
93	10.0
94	7.0
95	8.0
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	988985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.428858076826938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46822038718048	19.190706655363098
2	8.644261305543614	4.396273878353506
3	3.1728762483126816	2.4204785944103486
4	1.70666967429065	1.7359464372624558
5	1.086118473866311	1.3809376263283148
6	0.8096863812403	1.2353640045179508
7	0.6721862283805888	1.1965049741881302
8	0.5719264916406264	1.163475006904563
9	0.5032385786875909	1.1517104156607771
>10	6.0965555887194665	34.18221155049496
>50	0.9145688176890328	16.35340932124606
>100	0.34411100753357043	12.874789873627316
>500	0.0059880105719298795	0.9843636158997403
>1k	0.0031936056383626028	1.137951545244518
>5k	3.9920070479532535E-4	0.5958765004982444
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5870	0.5935378190771347	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2271	0.22962936748282328	RNA PCR Primer, Index 16 (96% over 26bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1675	0.16936556166170366	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1398	0.14135704788242492	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1284	0.12983007831261342	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	1275	0.12892005439920726	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1141	0.11537080946627098	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1116	0.11284296526236495	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1050	0.10616945656405305	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2845341435916622	0.0
2	0.0	0.0	0.0	0.40465730016127643	0.0
3	0.0	0.0	0.0	0.5119390081750481	0.0
4	0.0	0.0	0.0	1.0557288533193123	0.0
5	0.0	0.0	0.0	1.1562359388666157	0.0
6	0.0	0.0	0.0	1.8280358144966808	0.0
7	0.0	0.0	0.0	2.306303937875701	0.0
8	0.0	0.0	0.0	2.7568668887799106	0.0
9	0.0	0.0	0.0	3.380435496999449	0.0
10	0.0	0.0	0.0	4.271955590833025	0.0
11	0.0	0.0	0.0	5.161048954230853	0.0
12	0.0	0.0	0.0	5.56216727250666	0.0
13	0.0	0.0	0.0	5.744374282724207	0.0
14	0.0	0.0	0.0	5.961869998028281	0.0
15	0.0	0.0	0.0	6.145795942304484	0.0
16	0.0	0.0	0.0	6.495750693893234	0.0
17	0.0	0.0	0.0	6.910013802029353	0.0
18	0.0	0.0	0.0	7.454410329782555	0.0
19	0.0	0.0	0.0	7.688690930600565	0.0
20	0.0	0.0	0.0	7.951384500270479	0.0
21	0.0	0.0	0.0	8.251793505462672	0.0
22	0.0	0.0	0.0	8.585468940378266	0.0
23	0.0	0.0	0.0	8.926626794137423	0.0
24	0.0	0.0	0.0	9.188915908734712	0.0
25	0.0	0.0	0.0	9.429061108105785	0.0
26	0.0	0.0	0.0	9.645141230655673	0.0
27	0.0	0.0	0.0	9.90449804597643	0.0
28	0.0	0.0	0.0	10.137868622881035	0.0
29	0.0	0.0	0.0	10.376396001961607	0.0
30	0.0	0.0	0.0	10.691365389768297	0.0
31	0.0	0.0	0.0	10.92534264928184	0.0
32	0.0	0.0	0.0	11.187227308806504	0.0
33	0.0	0.0	0.0	11.439607274124482	0.0
34	0.0	0.0	0.0	11.695121766255301	0.0
35	0.0	0.0	0.0	11.971364580858152	0.0
36	0.0	0.0	0.0	12.222935636030881	0.0
37	0.0	0.0	0.0	12.45913739844386	0.0
38	1.01113768156241E-4	0.0	0.0	12.715056345647305	0.0
39	1.01113768156241E-4	0.0	0.0	12.971581975459689	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCCC	25	3.8904098E-5	45.0	12
CGAACCG	20	7.032809E-4	45.0	1
TCACACG	55	1.8189894E-12	45.0	1
ACGATAG	40	6.8139343E-9	45.0	1
TATTACG	20	7.032809E-4	45.0	1
GTATACG	45	3.8380676E-10	45.0	1
TATAGCG	20	7.032809E-4	45.0	1
CGCAATG	20	7.032809E-4	45.0	1
CGCGTAG	20	7.032809E-4	45.0	1
TCGAACG	25	3.8904098E-5	45.0	1
TACCGAC	20	7.032809E-4	45.0	20
AATTGCG	60	0.0	44.999996	1
ACGTAGA	30	2.165194E-6	44.999996	38
CGTAACG	75	0.0	42.0	1
AACGGGA	775	0.0	41.51613	4
TCTCGCG	60	3.6379788E-12	41.249996	1
TATCGCG	50	1.0804797E-9	40.5	1
GTAGGGA	390	0.0	40.384617	4
CATACGA	465	0.0	40.161293	18
ATTCACG	45	1.927765E-8	40.0	1
>>END_MODULE