##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731426
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41093152280614	34.0	31.0	34.0	31.0	34.0
2	32.586997180849465	34.0	31.0	34.0	31.0	34.0
3	32.66059724428719	34.0	31.0	34.0	31.0	34.0
4	36.15974411628791	37.0	35.0	37.0	35.0	37.0
5	36.011513126413334	37.0	35.0	37.0	35.0	37.0
6	35.98979801100863	37.0	35.0	37.0	35.0	37.0
7	36.03464465304761	37.0	35.0	37.0	35.0	37.0
8	36.123744575664524	37.0	36.0	37.0	35.0	37.0
9	37.78096212056995	39.0	38.0	39.0	35.0	39.0
10	37.37823648598765	39.0	37.0	39.0	35.0	39.0
11	37.34356038751699	39.0	37.0	39.0	34.0	39.0
12	37.322642345226996	39.0	37.0	39.0	34.0	39.0
13	37.29825026728609	39.0	37.0	39.0	34.0	39.0
14	38.58274111119922	40.0	38.0	41.0	34.0	41.0
15	38.59714311495626	40.0	38.0	41.0	34.0	41.0
16	38.59275032607537	40.0	38.0	41.0	34.0	41.0
17	38.5420493665798	40.0	38.0	41.0	34.0	41.0
18	38.476104212866375	40.0	38.0	41.0	34.0	41.0
19	38.4803356730551	40.0	38.0	41.0	34.0	41.0
20	38.39526486616555	40.0	38.0	41.0	34.0	41.0
21	38.37866031560267	40.0	38.0	41.0	34.0	41.0
22	38.30540068304928	40.0	38.0	41.0	34.0	41.0
23	38.21169332235934	40.0	37.0	41.0	34.0	41.0
24	38.16327010524646	40.0	37.0	41.0	34.0	41.0
25	38.10565798864137	40.0	37.0	41.0	33.0	41.0
26	38.06125431690971	40.0	37.0	41.0	33.0	41.0
27	38.00432169488096	40.0	37.0	41.0	33.0	41.0
28	37.891917979399146	40.0	37.0	41.0	33.0	41.0
29	37.827203298761596	40.0	37.0	41.0	33.0	41.0
30	37.78270939233771	40.0	37.0	41.0	33.0	41.0
31	37.704199741327216	40.0	37.0	41.0	33.0	41.0
32	37.6617497874016	40.0	37.0	41.0	33.0	41.0
33	37.57383932209137	40.0	36.0	41.0	33.0	41.0
34	37.473414945599416	40.0	36.0	41.0	32.0	41.0
35	37.417051075570186	40.0	36.0	41.0	32.0	41.0
36	37.334978521408864	40.0	36.0	41.0	32.0	41.0
37	37.270341770732784	40.0	36.0	41.0	32.0	41.0
38	37.2225843762732	39.0	35.0	41.0	32.0	41.0
39	37.15162162679478	39.0	35.0	41.0	32.0	41.0
40	37.05364862610845	39.0	35.0	41.0	31.0	41.0
41	36.96716961114316	39.0	35.0	41.0	31.0	41.0
42	36.88653807767293	39.0	35.0	41.0	31.0	41.0
43	36.81112921881366	39.0	35.0	41.0	31.0	41.0
44	36.72648224153913	39.0	35.0	41.0	31.0	41.0
45	36.60270348606694	39.0	35.0	41.0	31.0	41.0
46	36.46177467030157	39.0	35.0	41.0	30.0	41.0
47	36.34942290812741	39.0	35.0	40.0	30.0	41.0
48	36.269230243387575	39.0	35.0	40.0	30.0	41.0
49	36.15620445540629	38.0	35.0	40.0	30.0	41.0
50	36.068620749057324	38.0	35.0	40.0	29.0	41.0
51	35.06831449798066	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	2.0
11	3.0
12	7.0
13	11.0
14	19.0
15	27.0
16	56.0
17	114.0
18	168.0
19	293.0
20	587.0
21	832.0
22	1267.0
23	1714.0
24	2373.0
25	3081.0
26	3948.0
27	4953.0
28	6359.0
29	8175.0
30	10638.0
31	13588.0
32	17816.0
33	24170.0
34	37643.0
35	49612.0
36	58504.0
37	86405.0
38	150203.0
39	248760.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.29661783967209	31.623978365548943	20.10182848299076	26.977575311788204
2	24.298425267901333	30.661064824056023	19.58817433342539	25.45233557461725
3	21.682439508576397	30.88337029309869	20.605228690257114	26.8289615080678
4	21.95751860065133	31.45567699261443	18.95024240319595	27.636562003538295
5	19.36108369130985	34.307229986355416	16.431327297635033	29.900359024699696
6	19.921222379297426	39.790491450946504	18.533522188163943	21.754763981592124
7	86.58305829981433	5.5899024645008515	4.013119577373514	3.813919658311299
8	87.40063929912255	5.570351614517395	3.679114496886903	3.3498945894731658
9	78.89930081785444	10.161109941402138	6.337483217714437	4.602106023028988
10	33.038338806659866	39.39564631281907	13.46493015014506	14.101084730376003
11	23.761665568355514	28.940863463973116	29.41350184434242	17.88396912332895
12	26.918375884915218	24.772840998269135	29.955456874653073	18.35332624216257
13	24.24045631410423	26.260619666241013	29.105610136910638	20.393313882744117
14	21.734802973916707	27.40468618834988	28.69148758726105	22.169023250472364
15	20.221867967504572	30.446552351160612	29.09535619461162	20.236223486723194
16	22.02656181213137	28.61328418732722	28.695862602641963	20.664291397899444
17	21.500602931807183	28.63201472192676	29.18354009838316	20.683842247882904
18	21.844588516131502	29.044223202347197	28.534944068162737	20.576244213358564
19	21.632263550926545	29.216352713740008	28.24414226456265	20.907241470770796
20	22.46693445406644	28.251388383787283	29.429634713559537	19.852042448586733
21	23.767407776042962	28.796624675633627	27.706698968863563	19.72926857945985
22	22.49701268481022	28.2595915376265	27.749218649596813	21.494177127966466
23	21.943026362202055	29.77170622865471	27.54728434592153	20.73798306322171
24	20.831362297758076	29.39682209820269	29.380279071293607	20.391536532745622
25	20.823706020841477	31.02036296221354	26.596538815956777	21.559392200988206
26	22.237382865799137	29.63867841722881	27.06097404248687	21.062964674485187
27	21.025913762978075	28.812210667928127	27.665273041975535	22.49660252711826
28	20.546576140306744	29.805065720934177	29.43619723663091	20.212160902128172
29	20.950991624579927	29.709498978707348	27.355877423006564	21.983631973706157
30	21.924432546833174	28.823695083303026	28.12041136082119	21.13146100904261
31	22.05581972749123	29.42184171741229	26.753492492747043	21.768846062349436
32	22.37820367337229	29.176430698389172	27.46402233445352	20.981343293785017
33	20.573099671053534	30.32077065895935	27.729120922690743	21.37700874729638
34	20.17046153677884	29.627194001853912	29.03984818696628	21.162496274400965
35	22.303281534974147	28.931293117827366	28.308263583739162	20.457161763459325
36	22.272929865769058	31.445012892623453	26.90880553876947	19.37325170283802
37	21.132007885965223	31.911772346074653	27.035817704046615	19.920402063913507
38	20.489154063432256	29.81901108246084	28.852132683278963	20.839702170827945
39	21.131187570581304	30.111316797598114	26.900739104160916	21.85675652765967
40	21.571970370208334	30.261297793625058	27.970567084024907	20.19616475214171
41	20.15063724833408	28.54000267969692	30.60418962410415	20.705170447864855
42	20.255500898245344	29.849772909357885	27.701777076560035	22.192949115836736
43	21.252457528171	29.017973110061718	27.771914041885303	21.957655319881983
44	20.828217755453046	28.995961313926493	27.875820657182	22.30000027343846
45	20.780776182416265	29.746960047906416	27.893594157166955	21.578669612510357
46	20.3365754020229	30.865323354652418	27.96455143787615	20.833549805448534
47	20.038937636890132	30.330477724335747	29.08332490231411	20.54725973646001
48	20.072297129169595	29.226059779116408	29.69104188256912	21.010601209144873
49	19.631924487234524	31.24280515048686	27.777109372650138	21.34816098962848
50	19.849308063973663	28.817405998692962	29.573053186515107	21.760232750818265
51	20.534544848009233	28.606858383486504	28.490920475892302	22.367676292611964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4240.0
1	3349.5
2	2459.0
3	1691.0
4	923.0
5	917.0
6	911.0
7	985.5
8	1060.0
9	1177.0
10	1294.0
11	1635.5
12	1977.0
13	2215.0
14	2453.0
15	2383.0
16	2313.0
17	2287.0
18	2261.0
19	2355.5
20	2450.0
21	3064.5
22	3679.0
23	5029.5
24	6380.0
25	6503.0
26	8709.5
27	10793.0
28	13269.0
29	15745.0
30	18247.5
31	20750.0
32	24011.5
33	27273.0
34	30513.5
35	33754.0
36	35480.5
37	37207.0
38	38968.5
39	40730.0
40	41308.5
41	41887.0
42	42929.5
43	43972.0
44	46403.0
45	48834.0
46	48895.5
47	48957.0
48	49763.5
49	50570.0
50	49829.0
51	49088.0
52	44978.5
53	40869.0
54	37219.0
55	33569.0
56	30784.0
57	27999.0
58	26449.0
59	24899.0
60	24925.5
61	24952.0
62	22775.0
63	20598.0
64	18081.5
65	15565.0
66	13348.5
67	11132.0
68	9543.5
69	7955.0
70	6618.5
71	5282.0
72	4536.0
73	3790.0
74	3336.5
75	2217.0
76	1551.0
77	1187.0
78	823.0
79	657.5
80	492.0
81	393.0
82	294.0
83	201.0
84	108.0
85	80.0
86	52.0
87	32.0
88	12.0
89	9.0
90	6.0
91	4.5
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	731426.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.658788839135358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.00463752075194	16.31537877021201
2	8.014752816292967	3.6320918332449548
3	3.2276125566457634	2.194013741267345
4	1.9968500392996535	1.8098481353563993
5	1.3775125117323934	1.5606382563305634
6	1.1392539040135443	1.548846818712209
7	0.9361119331685741	1.4847813836423056
8	0.8132433204770507	1.4741686938821421
9	0.7020354330384911	1.431654537132911
>10	8.332552217189324	40.60125961635695
>50	1.2055876007549446	18.80307925357048
>100	0.2425729578983792	7.54423417489736
>500	0.006670756342205427	1.1198934215874086
>1k	6.06432394745948E-4	0.4801113638069209
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3494	0.47769699190348713	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	980	0.13398484604047436	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	902	0.12332074604949783	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	849	0.11607462682485994	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	825	0.11279336528917484	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	821	0.11224648836656068	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	772	0.10554724606453696	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7343846130709054E-4	0.0	0.0	0.0936526729976785	0.0
2	2.7343846130709054E-4	0.0	0.0	0.14095752680380516	0.0
3	2.7343846130709054E-4	0.0	0.0	0.1890826959938531	0.0
4	2.7343846130709054E-4	0.0	0.0	0.3999037496616199	0.0
5	2.7343846130709054E-4	0.0	0.0	0.4413296765496441	0.0
6	2.7343846130709054E-4	0.0	0.0	0.7422487032180972	0.0
7	2.7343846130709054E-4	0.0	0.0	0.9676987145657934	0.0
8	2.7343846130709054E-4	0.0	0.0	1.208324560516033	0.0
9	2.7343846130709054E-4	0.0	0.0	1.49666541796436	0.0
10	2.7343846130709054E-4	0.0	0.0	1.950983421426091	0.0
11	4.101576919606358E-4	0.0	0.0	2.3126057865047183	0.0
12	4.101576919606358E-4	0.0	0.0	2.4880165594332166	0.0
13	4.101576919606358E-4	0.0	0.0	2.5640324516765878	0.0
14	4.101576919606358E-4	0.0	0.0	2.6426460093023763	0.0
15	4.101576919606358E-4	0.0	0.0	2.7226267592347004	0.0
16	4.101576919606358E-4	0.0	0.0	2.864951478345041	0.0
17	4.101576919606358E-4	0.0	0.0	3.039952093581579	0.0
18	4.101576919606358E-4	0.0	0.0	3.253780970323724	0.0
19	4.101576919606358E-4	0.0	0.0	3.3494844317812054	0.0
20	4.101576919606358E-4	0.0	0.0	3.460910604763845	0.0
21	4.101576919606358E-4	0.0	0.0	3.595579046957587	0.0
22	4.101576919606358E-4	0.0	0.0	3.749524900673479	0.0
23	4.101576919606358E-4	0.0	0.0	3.9111270313059694	0.0
24	4.101576919606358E-4	0.0	0.0	4.0347212158167745	0.0
25	4.101576919606358E-4	0.0	0.0	4.141225496495887	0.0
26	4.101576919606358E-4	0.0	0.0	4.24239772717951	0.0
27	4.101576919606358E-4	0.0	0.0	4.362027054001362	0.0
28	4.101576919606358E-4	0.0	0.0	4.474000103906615	0.0
29	4.101576919606358E-4	0.0	0.0	4.596090376880231	0.0
30	4.101576919606358E-4	0.0	0.0	4.753044053670501	0.0
31	4.101576919606358E-4	0.0	0.0	4.880603095870259	0.0
32	4.101576919606358E-4	0.0	0.0	5.012400434220276	0.0
33	4.101576919606358E-4	0.0	0.0	5.155135311022578	0.0
34	4.101576919606358E-4	0.0	0.0	5.280233407070572	0.0
35	4.101576919606358E-4	0.0	0.0	5.42324172233418	0.0
36	4.101576919606358E-4	0.0	0.0	5.57171880682393	0.0
37	4.101576919606358E-4	0.0	0.0	5.709668510553358	0.0
38	4.101576919606358E-4	0.0	0.0	5.870040168109966	0.0
39	4.101576919606358E-4	0.0	0.0	6.032599333357031	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	40	6.8084773E-9	45.000004	6
AAATCGT	20	7.031601E-4	45.000004	43
CGTATAG	20	7.031601E-4	45.000004	1
CGTGAGT	20	7.031601E-4	45.000004	34
CGATTAG	20	7.031601E-4	45.000004	1
AATTGCG	20	7.031601E-4	45.000004	1
TCATACG	40	6.8084773E-9	45.000004	1
CCAACGA	20	7.031601E-4	45.000004	27
ATACGAG	20	7.031601E-4	45.000004	1
GGCGAAT	20	7.031601E-4	45.000004	16
GCGAATT	25	3.8894097E-5	45.0	17
CGAATTA	25	3.8894097E-5	45.0	18
TATAGCG	25	3.8894097E-5	45.0	1
GCGAGAC	135	0.0	43.333332	21
ATAACGG	130	0.0	43.26923	2
TTAACGG	60	3.6379788E-12	41.249996	2
TAACGGG	390	0.0	40.96154	3
CCGACAG	55	6.002665E-11	40.90909	1
CAAACGC	50	1.0804797E-9	40.5	34
AAGTACG	45	1.9266736E-8	40.0	1
>>END_MODULE