FastQCFastQC Report
Sat 18 Jun 2016
SRR3554132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791647
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46580.5883935643032816No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA10570.13351910636937928No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9520.12025561898169258No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9060.11444494831661081No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT9000.11368703475160015No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8950.11305544011409126No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT8170.10320256376895258RNA PCR Primer, Index 41 (96% over 27bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7980.10080250414641881No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG351.2112832E-745.0000041
TTAGGCG351.2112832E-745.0000041
CGATTCG207.0319534E-445.00000411
TAACGCG302.1646429E-645.0000041
ATGTCGC207.0319534E-445.00000415
GCCGATT207.0319534E-445.0000049
ACGATAG207.0319534E-445.0000041
AGCGACG406.8102963E-945.0000041
ACTCGAT207.0319534E-445.00000441
TCGACGG207.0319534E-445.0000042
TTATTCG302.1646429E-645.00000424
TGTAACG351.2112832E-745.0000041
CGATAAT302.1646429E-645.00000437
GTAACGT207.0319534E-445.00000421
ATACCCG650.045.0000041
TCAACGT207.0319534E-445.00000427
CGACGAA253.889702E-545.034
CAATACG453.8380676E-1045.01
CGCTACG1100.042.9545441
AACGGGA8600.041.8604664