##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	791647
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.300222194993474	34.0	31.0	34.0	30.0	34.0
2	32.48084436623899	34.0	31.0	34.0	30.0	34.0
3	32.57737223787875	34.0	31.0	34.0	31.0	34.0
4	36.097581371495124	37.0	35.0	37.0	35.0	37.0
5	35.92854643546934	37.0	35.0	37.0	35.0	37.0
6	35.90363255339817	37.0	35.0	37.0	35.0	37.0
7	35.985648906646524	37.0	35.0	37.0	35.0	37.0
8	36.08415493269096	37.0	35.0	37.0	35.0	37.0
9	37.72152739794378	39.0	38.0	39.0	35.0	39.0
10	37.30166854671337	39.0	37.0	39.0	34.0	39.0
11	37.27086567624206	39.0	37.0	39.0	34.0	39.0
12	37.198801991291575	39.0	37.0	39.0	34.0	39.0
13	37.19028430601013	39.0	37.0	39.0	33.0	39.0
14	38.44858251215504	40.0	38.0	41.0	34.0	41.0
15	38.46189021116735	40.0	38.0	41.0	34.0	41.0
16	38.44887557206685	40.0	38.0	41.0	34.0	41.0
17	38.407154956691556	40.0	38.0	41.0	34.0	41.0
18	38.34717494034589	40.0	38.0	41.0	34.0	41.0
19	38.33036820704177	40.0	38.0	41.0	34.0	41.0
20	38.25111950149498	40.0	37.0	41.0	34.0	41.0
21	38.215465984207604	40.0	37.0	41.0	34.0	41.0
22	38.150073201818486	40.0	37.0	41.0	33.0	41.0
23	38.07076512637577	40.0	37.0	41.0	33.0	41.0
24	37.98909993974587	40.0	37.0	41.0	33.0	41.0
25	37.94710394910863	40.0	37.0	41.0	33.0	41.0
26	37.83694626519143	40.0	37.0	41.0	33.0	41.0
27	37.75818136113697	40.0	37.0	41.0	33.0	41.0
28	37.664164709775946	40.0	36.0	41.0	33.0	41.0
29	37.539173394202216	40.0	36.0	41.0	33.0	41.0
30	37.508495579469134	40.0	36.0	41.0	33.0	41.0
31	37.473156596311235	40.0	36.0	41.0	32.0	41.0
32	37.41664656090404	40.0	36.0	41.0	32.0	41.0
33	37.30671625105634	40.0	36.0	41.0	32.0	41.0
34	37.23003434611639	39.0	36.0	41.0	32.0	41.0
35	37.17966341058578	39.0	35.0	41.0	31.0	41.0
36	37.08541812196598	39.0	35.0	41.0	31.0	41.0
37	36.996982240821985	39.0	35.0	41.0	31.0	41.0
38	36.927355247983	39.0	35.0	41.0	31.0	41.0
39	36.84894782649337	39.0	35.0	41.0	31.0	41.0
40	36.74966114947698	39.0	35.0	41.0	31.0	41.0
41	36.683631719693246	39.0	35.0	41.0	31.0	41.0
42	36.58743101407572	39.0	35.0	41.0	30.0	41.0
43	36.51676820603122	39.0	35.0	41.0	30.0	41.0
44	36.39737913489219	39.0	35.0	40.0	30.0	41.0
45	36.24864491370523	39.0	35.0	40.0	30.0	41.0
46	36.13535957314308	38.0	35.0	40.0	30.0	41.0
47	35.95815053931866	38.0	35.0	40.0	29.0	41.0
48	35.87181786831757	38.0	35.0	40.0	29.0	41.0
49	35.72545212702126	38.0	34.0	40.0	28.0	41.0
50	35.62349759425602	38.0	34.0	40.0	28.0	41.0
51	34.54453057991756	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	0.0
11	8.0
12	13.0
13	13.0
14	22.0
15	26.0
16	66.0
17	103.0
18	217.0
19	386.0
20	672.0
21	960.0
22	1437.0
23	2046.0
24	2817.0
25	3720.0
26	4883.0
27	6260.0
28	7799.0
29	9838.0
30	12871.0
31	16428.0
32	21583.0
33	29115.0
34	43497.0
35	56982.0
36	67270.0
37	97983.0
38	164250.0
39	240288.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.95278072171056	32.53811357840048	18.62294684373212	25.88615885615685
2	24.469997359934414	31.031381411159266	19.778512392518383	24.720108836387936
3	20.954415288632433	30.619455388575968	20.54514196352667	27.880987359264925
4	21.9467767830864	31.43951786591751	19.28081581816138	27.33288953283471
5	19.250751913415954	34.054698621986816	16.59969658193614	30.094852882661083
6	20.36299007006911	39.52961357776888	18.428668333234384	21.67872801892763
7	86.1525402104726	5.992822558539349	4.1133232362403955	3.741313994747659
8	87.18102891819207	5.871177431355137	3.7944942632259075	3.153299387226883
9	78.25874411195899	10.494071221137705	6.675955318468964	4.57122934843434
10	32.473311968592064	39.936613162179604	13.972641846681666	13.617433022546665
11	24.74309888119326	29.42965741043672	27.853197195214534	17.974046513155486
12	25.529307885964325	25.579203862327525	30.01918784508752	18.872300406620628
13	24.039881411790862	26.651525237890123	28.597468316055007	20.711125034264008
14	20.771252843754855	28.649764352040748	29.29954891510989	21.279433889094506
15	20.9520152290099	30.568422541865253	28.609342295240175	19.870219933884673
16	22.621067218090893	29.00661532223327	27.94844166655087	20.423875793124964
17	21.19000008842325	29.050321671148883	28.74968262369465	21.009995616733214
18	21.998567543362128	28.774441133485002	28.187942353094247	21.039048970058623
19	21.673675261827558	29.50052232876522	28.250722860062627	20.575079549344593
20	22.415420004117998	28.723913562484288	29.555850019010997	19.304816414386718
21	24.099124988789196	28.97958307174789	27.57239021937808	19.348901720084836
22	22.827093388846293	28.034212218324583	27.595885539893413	21.542808852935718
23	21.38617338283351	29.04185830300626	28.58193108797229	20.990037226187937
24	20.44535001080027	29.292727693024794	29.6057459953742	20.656176300800734
25	21.433416661719175	30.66985664064918	26.863614717165607	21.03311198046604
26	22.0320420591501	29.735475533918525	27.639465569881526	20.593016837049845
27	21.596873353906474	28.897602087799235	28.09168733033789	21.4138372279564
28	20.98384759874035	29.447342060286974	28.941055798859843	20.627754542112836
29	21.009111384240704	29.476774370394885	27.68165609166712	21.832458153697292
30	21.979746023164363	29.40755159812391	28.76585144641488	19.846850932296846
31	21.962819286879125	29.51833329754297	27.41108094895831	21.107766466619594
32	21.960671865111596	28.800336513622867	27.505441187802138	21.7335504334634
33	20.384337968816908	30.053293955513	28.019685541661875	21.542682534008215
34	19.73783769786281	29.48561669532001	29.696569304247976	21.0799763025692
35	21.922144592223557	29.368266411670856	28.31350336703101	20.396085629074577
36	21.849005933200026	30.969358817755893	27.586158982475773	19.59547626656831
37	20.781105720099994	31.920540341844283	27.394785807310583	19.903568130745143
38	20.648976121933135	29.95754420846665	29.125986708722447	20.267492960877764
39	20.8312543343182	29.229441910346406	28.246049059745065	21.693254695590333
40	21.374425722575847	29.721706770820834	28.764967213922365	20.138900292680955
41	20.028623868971902	28.89318092533667	29.533870525625687	21.544324680065735
42	19.977212065478682	30.2734678461486	27.600054064500974	22.14926602387175
43	20.893024289866567	28.87259094015388	28.414432190104932	21.819952579874617
44	20.73095710588179	29.704148439898088	28.380957674316964	21.183936779903163
45	21.30646613957989	29.9607021816542	28.08436083254279	20.648470846223127
46	19.927063451260473	30.546190410624934	28.72302932999178	20.803716808122815
47	20.55335269381429	30.12996954450658	28.32790372476622	20.988774036912915
48	20.797653499602728	28.67831242965615	29.416393923049032	21.107640147692088
49	19.53673796527998	30.16748626597461	29.313317678207586	20.98245809053783
50	20.469729563808112	28.980340985312896	28.751830045462185	21.798099405416806
51	20.719209445624124	28.148909804496196	29.19141991316837	21.940460836711313
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5695.0
1	4531.5
2	3368.0
3	2224.0
4	1080.0
5	1039.0
6	998.0
7	1152.5
8	1307.0
9	1546.0
10	1785.0
11	2235.0
12	2685.0
13	3079.0
14	3473.0
15	3481.5
16	3490.0
17	3496.5
18	3503.0
19	3649.5
20	3796.0
21	3852.5
22	3909.0
23	4568.5
24	5228.0
25	7361.5
26	10487.0
27	11479.0
28	14248.5
29	17018.0
30	18803.0
31	20588.0
32	25459.5
33	30331.0
34	31443.0
35	32555.0
36	34746.5
37	36938.0
38	40149.5
39	43361.0
40	44230.5
41	45100.0
42	44716.0
43	44332.0
44	46786.0
45	49240.0
46	52665.0
47	56090.0
48	55916.0
49	55742.0
50	54966.5
51	54191.0
52	50343.0
53	46495.0
54	41959.5
55	37424.0
56	35334.5
57	33245.0
58	30905.0
59	28565.0
60	27792.0
61	27019.0
62	23988.5
63	20958.0
64	18274.0
65	15590.0
66	13505.5
67	11421.0
68	9603.0
69	7785.0
70	6862.0
71	5939.0
72	4826.0
73	3713.0
74	3363.5
75	2391.0
76	1768.0
77	1381.0
78	994.0
79	758.0
80	522.0
81	365.0
82	208.0
83	156.0
84	104.0
85	83.5
86	63.0
87	43.0
88	23.0
89	17.0
90	11.0
91	7.5
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	791647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.192813200817724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2546794771378	17.964295898769883
2	7.939117728147961	3.8413918435276804
3	3.1085277226285926	2.2561209156935056
4	1.8794358026367186	1.8187535718447625
5	1.3066549579598898	1.5805829657922976
6	1.012822160740478	1.4701810404265778
7	0.7604038018673636	1.287741499503812
8	0.676454699183896	1.3092273740937073
9	0.577631662579064	1.257708142048776
>10	7.1403986896183085	37.63880293011644
>50	1.0115796266727997	16.899421323072495
>100	0.32546931423820785	10.897387357006048
>500	0.005774455575194011	1.0525784492327592
>1k	0.0010499010136716383	0.725806688871286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4658	0.5883935643032816	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1057	0.13351910636937928	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	952	0.12025561898169258	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	906	0.11444494831661081	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	900	0.11368703475160015	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	895	0.11305544011409126	No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	817	0.10320256376895258	RNA PCR Primer, Index 41 (96% over 27bp)
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	798	0.10080250414641881	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5263785500355586E-4	0.0	0.0	0.12732947892179217	0.0
2	2.5263785500355586E-4	0.0	0.0	0.18253085024006913	0.0
3	2.5263785500355586E-4	0.0	0.0	0.24467976257094387	0.0
4	2.5263785500355586E-4	0.0	0.0	0.5435503450401504	0.0
5	2.5263785500355586E-4	0.0	0.0	0.5953411053158795	0.0
6	2.5263785500355586E-4	0.0	0.0	0.976697947443747	0.0
7	2.5263785500355586E-4	0.0	0.0	1.2351464731123847	0.0
8	2.5263785500355586E-4	0.0	0.0	1.4925844473610081	0.0
9	2.5263785500355586E-4	0.0	0.0	1.8169714531855738	0.0
10	2.5263785500355586E-4	0.0	0.0	2.3879330054936103	0.0
11	2.5263785500355586E-4	0.0	0.0	2.8549340804676833	0.0
12	2.5263785500355586E-4	0.0	0.0	3.084329252810912	0.0
13	2.5263785500355586E-4	0.0	0.0	3.1875318165798645	0.0
14	2.5263785500355586E-4	0.0	0.0	3.306650565214041	0.0
15	2.5263785500355586E-4	0.0	0.0	3.4069477936504526	0.0
16	2.5263785500355586E-4	0.0	0.0	3.6061527423207567	0.0
17	2.5263785500355586E-4	0.0	0.0	3.8485587641966683	0.0
18	2.5263785500355586E-4	0.0	0.0	4.139218616378259	0.0
19	2.5263785500355586E-4	0.0	0.0	4.2910539672353964	0.0
20	2.5263785500355586E-4	0.0	0.0	4.469416292867907	0.0
21	2.5263785500355586E-4	0.0	0.0	4.644620645312873	0.0
22	2.5263785500355586E-4	0.0	0.0	4.843699275055675	0.0
23	2.5263785500355586E-4	0.0	0.0	5.047830661898548	0.0
24	2.5263785500355586E-4	0.0	0.0	5.213561094780881	0.0
25	2.5263785500355586E-4	0.0	0.0	5.361227921030459	0.0
26	2.5263785500355586E-4	0.0	0.0	5.49487334632734	0.0
27	2.5263785500355586E-4	0.0	0.0	5.640013794026883	0.0
28	2.5263785500355586E-4	0.0	0.0	5.7939965666515505	0.0
29	2.5263785500355586E-4	0.0	0.0	5.95808485347636	0.0
30	2.5263785500355586E-4	0.0	0.0	6.160195137479205	0.0
31	2.5263785500355586E-4	0.0	0.0	6.3185990725664345	0.0
32	2.5263785500355586E-4	0.0	0.0	6.494056062866404	0.0
33	2.5263785500355586E-4	0.0	0.0	6.682144945916551	0.0
34	3.789567825053338E-4	0.0	0.0	6.856212428014001	0.0
35	3.789567825053338E-4	0.0	0.0	7.050743576366739	0.0
36	3.789567825053338E-4	0.0	0.0	7.229484858781755	0.0
37	3.789567825053338E-4	0.0	0.0	7.414163130789355	0.0
38	3.789567825053338E-4	0.0	0.0	7.604652073462035	0.0
39	3.789567825053338E-4	0.0	0.0	7.805878124972367	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	35	1.2112832E-7	45.000004	1
TTAGGCG	35	1.2112832E-7	45.000004	1
CGATTCG	20	7.0319534E-4	45.000004	11
TAACGCG	30	2.1646429E-6	45.000004	1
ATGTCGC	20	7.0319534E-4	45.000004	15
GCCGATT	20	7.0319534E-4	45.000004	9
ACGATAG	20	7.0319534E-4	45.000004	1
AGCGACG	40	6.8102963E-9	45.000004	1
ACTCGAT	20	7.0319534E-4	45.000004	41
TCGACGG	20	7.0319534E-4	45.000004	2
TTATTCG	30	2.1646429E-6	45.000004	24
TGTAACG	35	1.2112832E-7	45.000004	1
CGATAAT	30	2.1646429E-6	45.000004	37
GTAACGT	20	7.0319534E-4	45.000004	21
ATACCCG	65	0.0	45.000004	1
TCAACGT	20	7.0319534E-4	45.000004	27
CGACGAA	25	3.889702E-5	45.0	34
CAATACG	45	3.8380676E-10	45.0	1
CGCTACG	110	0.0	42.954544	1
AACGGGA	860	0.0	41.860466	4
>>END_MODULE