Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554131_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 773445 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4626 | 0.5981032911196013 | No Hit |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 959 | 0.12399071685769511 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 917 | 0.11856046648436541 | No Hit |
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 916 | 0.11843117480880995 | No Hit |
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT | 900 | 0.11636250799992243 | No Hit |
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT | 850 | 0.10989792422214896 | RNA PCR Primer, Index 41 (96% over 27bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 829 | 0.1071827990354841 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCACGCG | 40 | 6.8102963E-9 | 45.000004 | 1 |
TATTCGA | 35 | 1.2112469E-7 | 45.000004 | 25 |
ACGTCGA | 20 | 7.031853E-4 | 45.000004 | 27 |
CATAACG | 40 | 6.8102963E-9 | 45.000004 | 1 |
CGCAATA | 20 | 7.031853E-4 | 45.000004 | 35 |
AATACGG | 35 | 1.2112469E-7 | 45.000004 | 2 |
TAATCCG | 40 | 6.8102963E-9 | 45.000004 | 1 |
ATTAGCG | 25 | 3.8896178E-5 | 45.0 | 1 |
TAGCGTC | 25 | 3.8896178E-5 | 45.0 | 45 |
TAACGCG | 25 | 3.8896178E-5 | 45.0 | 1 |
ACGATAG | 45 | 3.8380676E-10 | 45.0 | 1 |
CAATACG | 25 | 3.8896178E-5 | 45.0 | 1 |
CGAGGGT | 125 | 0.0 | 43.199997 | 4 |
ATACCCG | 80 | 0.0 | 42.187504 | 1 |
CGAACAG | 70 | 0.0 | 41.785717 | 1 |
ACCGGGT | 105 | 0.0 | 40.714287 | 4 |
ACTACGG | 185 | 0.0 | 40.135136 | 2 |
CTAGCGG | 185 | 0.0 | 40.135136 | 2 |
CGTGATA | 45 | 1.9268555E-8 | 40.0 | 18 |
TCTAGCG | 45 | 1.9268555E-8 | 40.0 | 1 |