FastQCFastQC Report
Sat 18 Jun 2016
SRR3554131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773445
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46260.5981032911196013No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA9590.12399071685769511No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9170.11856046648436541No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9160.11843117480880995No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT9000.11636250799992243No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT8500.10989792422214896RNA PCR Primer, Index 41 (96% over 27bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8290.1071827990354841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG406.8102963E-945.0000041
TATTCGA351.2112469E-745.00000425
ACGTCGA207.031853E-445.00000427
CATAACG406.8102963E-945.0000041
CGCAATA207.031853E-445.00000435
AATACGG351.2112469E-745.0000042
TAATCCG406.8102963E-945.0000041
ATTAGCG253.8896178E-545.01
TAGCGTC253.8896178E-545.045
TAACGCG253.8896178E-545.01
ACGATAG453.8380676E-1045.01
CAATACG253.8896178E-545.01
CGAGGGT1250.043.1999974
ATACCCG800.042.1875041
CGAACAG700.041.7857171
ACCGGGT1050.040.7142874
ACTACGG1850.040.1351362
CTAGCGG1850.040.1351362
CGTGATA451.9268555E-840.018
TCTAGCG451.9268555E-840.01