FastQCFastQC Report
Sat 18 Jun 2016
SRR3554128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260013
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110224.239018818289854No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24110.9272613292412302No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17370.668043520900878No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT16620.6391988092903047RNA PCR Primer, Index 38 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11620.44690073188648255No Hit
AAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT4730.18191398122401573RNA PCR Primer, Index 27 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4150.15960740424517236No Hit
ATTAATGGGAACATTTATCTATTAAAATTGCCATTTCATTAAGCAATTTTC3490.13422405802786783No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT3450.13268567340863727No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.13268567340863727No Hit
ACCAAGGGATTGTCTTTTAAGGTAATCTTTTCTGAACATTTATCTATCATG3180.12230157722883087No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG2980.11460965413267797No Hit
AATACTGGGATCACTTCTGTGTTAAAATGGAAGGTTTTTTGTGATGTATGA2790.10730232719133273No Hit
GGCTGAGGGATATATTTTCTTTTTATTCATAAATTACTACACATTGTGAGT2790.10730232719133273No Hit
AACTATGGGTGGTGATGAAGACAGGTATGTACATTTTTTTAAATTTTGAAT2770.10653313488171745No Hit
CTCGATGGGGTCTTCCTTTGCCTTTCCTCTTGATAGTGTTTTCTGATGAGC2730.10499475026248686No Hit
GCAGCAGGGAGAAGCTATTTTTTACTTTTTTTACTTGGAAATAAATCCTCT2670.10268717333364102No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAT207.023207E-445.00000422
AACGTAG406.7793735E-945.0000041
CGGAAGA207.023207E-445.00000419
AGGTAAG207.023207E-445.00000418
TGGAGGT207.023207E-445.00000415
TTGGCGA207.023207E-445.00000413
TCGTTCA406.7793735E-945.00000416
ATCTCGC207.023207E-445.00000419
TCCGCTT207.023207E-445.00000419
TCACCGG207.023207E-445.0000042
GGTCGTT207.023207E-445.0000048
CGTGCTT207.023207E-445.00000411
CCTAGTT207.023207E-445.00000429
TTAGCGG207.023207E-445.0000042
TAGGTTC207.023207E-445.00000410
GACGTGC207.023207E-445.0000049
CGTTGGG406.7793735E-945.0000043
GGCGTAA207.023207E-445.00000436
ATTACGG207.023207E-445.0000042
GCTATAA207.023207E-445.00000416