Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554128_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 260013 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 11022 | 4.239018818289854 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2411 | 0.9272613292412302 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1737 | 0.668043520900878 | No Hit |
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT | 1662 | 0.6391988092903047 | RNA PCR Primer, Index 38 (95% over 24bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1162 | 0.44690073188648255 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT | 473 | 0.18191398122401573 | RNA PCR Primer, Index 27 (95% over 21bp) |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 415 | 0.15960740424517236 | No Hit |
ATTAATGGGAACATTTATCTATTAAAATTGCCATTTCATTAAGCAATTTTC | 349 | 0.13422405802786783 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT | 345 | 0.13268567340863727 | No Hit |
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 345 | 0.13268567340863727 | No Hit |
ACCAAGGGATTGTCTTTTAAGGTAATCTTTTCTGAACATTTATCTATCATG | 318 | 0.12230157722883087 | No Hit |
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 298 | 0.11460965413267797 | No Hit |
AATACTGGGATCACTTCTGTGTTAAAATGGAAGGTTTTTTGTGATGTATGA | 279 | 0.10730232719133273 | No Hit |
GGCTGAGGGATATATTTTCTTTTTATTCATAAATTACTACACATTGTGAGT | 279 | 0.10730232719133273 | No Hit |
AACTATGGGTGGTGATGAAGACAGGTATGTACATTTTTTTAAATTTTGAAT | 277 | 0.10653313488171745 | No Hit |
CTCGATGGGGTCTTCCTTTGCCTTTCCTCTTGATAGTGTTTTCTGATGAGC | 273 | 0.10499475026248686 | No Hit |
GCAGCAGGGAGAAGCTATTTTTTACTTTTTTTACTTGGAAATAAATCCTCT | 267 | 0.10268717333364102 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CAGGTAT | 20 | 7.023207E-4 | 45.000004 | 22 |
AACGTAG | 40 | 6.7793735E-9 | 45.000004 | 1 |
CGGAAGA | 20 | 7.023207E-4 | 45.000004 | 19 |
AGGTAAG | 20 | 7.023207E-4 | 45.000004 | 18 |
TGGAGGT | 20 | 7.023207E-4 | 45.000004 | 15 |
TTGGCGA | 20 | 7.023207E-4 | 45.000004 | 13 |
TCGTTCA | 40 | 6.7793735E-9 | 45.000004 | 16 |
ATCTCGC | 20 | 7.023207E-4 | 45.000004 | 19 |
TCCGCTT | 20 | 7.023207E-4 | 45.000004 | 19 |
TCACCGG | 20 | 7.023207E-4 | 45.000004 | 2 |
GGTCGTT | 20 | 7.023207E-4 | 45.000004 | 8 |
CGTGCTT | 20 | 7.023207E-4 | 45.000004 | 11 |
CCTAGTT | 20 | 7.023207E-4 | 45.000004 | 29 |
TTAGCGG | 20 | 7.023207E-4 | 45.000004 | 2 |
TAGGTTC | 20 | 7.023207E-4 | 45.000004 | 10 |
GACGTGC | 20 | 7.023207E-4 | 45.000004 | 9 |
CGTTGGG | 40 | 6.7793735E-9 | 45.000004 | 3 |
GGCGTAA | 20 | 7.023207E-4 | 45.000004 | 36 |
ATTACGG | 20 | 7.023207E-4 | 45.000004 | 2 |
GCTATAA | 20 | 7.023207E-4 | 45.000004 | 16 |