##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260013
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98256241034102	33.0	31.0	34.0	30.0	34.0
2	32.182721633149114	34.0	31.0	34.0	30.0	34.0
3	32.29417759881237	34.0	31.0	34.0	30.0	34.0
4	35.86856426409449	37.0	35.0	37.0	35.0	37.0
5	35.66675512378227	37.0	35.0	37.0	33.0	37.0
6	35.63697199755397	37.0	35.0	37.0	33.0	37.0
7	35.61019641325626	37.0	35.0	37.0	35.0	37.0
8	35.761315780364825	37.0	35.0	37.0	35.0	37.0
9	37.41505617026841	39.0	37.0	39.0	35.0	39.0
10	36.940483745043515	39.0	37.0	39.0	33.0	39.0
11	36.984935368616185	39.0	37.0	39.0	33.0	39.0
12	36.98743908958398	39.0	37.0	39.0	33.0	39.0
13	36.93059193194186	39.0	37.0	39.0	33.0	39.0
14	38.05670101110329	40.0	37.0	41.0	33.0	41.0
15	38.092718441001026	40.0	37.0	41.0	33.0	41.0
16	38.09739128428194	40.0	37.0	41.0	33.0	41.0
17	38.01332625676409	39.0	37.0	41.0	33.0	41.0
18	37.783760811959404	39.0	37.0	41.0	33.0	41.0
19	37.60238526535212	39.0	36.0	41.0	33.0	41.0
20	37.52246233842154	39.0	35.0	41.0	33.0	41.0
21	37.470772615215395	39.0	35.0	41.0	32.0	41.0
22	37.40358751293205	39.0	35.0	41.0	32.0	41.0
23	37.23190763538746	39.0	35.0	41.0	32.0	41.0
24	37.14188136747009	39.0	35.0	41.0	32.0	41.0
25	37.06429678516074	39.0	35.0	41.0	32.0	41.0
26	36.99121197786265	39.0	35.0	41.0	32.0	41.0
27	36.88947091106983	39.0	35.0	41.0	31.0	41.0
28	36.82530873456327	39.0	35.0	40.0	31.0	41.0
29	36.67878144554311	39.0	35.0	40.0	31.0	41.0
30	36.558483614280824	39.0	35.0	40.0	31.0	41.0
31	36.543288220204374	39.0	35.0	40.0	31.0	41.0
32	36.41539461488464	38.0	35.0	40.0	30.0	41.0
33	36.24623768811559	38.0	35.0	40.0	30.0	41.0
34	36.20586662974544	38.0	35.0	40.0	30.0	41.0
35	36.147088799406184	38.0	35.0	40.0	30.0	41.0
36	36.00826112540527	38.0	35.0	40.0	30.0	41.0
37	35.93834154446124	38.0	35.0	40.0	30.0	41.0
38	35.87855607219639	38.0	35.0	40.0	29.0	41.0
39	35.75830054651114	38.0	35.0	40.0	29.0	41.0
40	35.6855003403676	38.0	35.0	40.0	28.0	41.0
41	35.61677300750347	38.0	35.0	40.0	28.0	41.0
42	35.454496505943936	38.0	34.0	40.0	27.0	41.0
43	35.34959790472015	38.0	34.0	40.0	27.0	41.0
44	35.259013972378305	38.0	34.0	40.0	26.0	41.0
45	35.16669551137828	38.0	34.0	40.0	26.0	41.0
46	35.064004492083086	38.0	34.0	40.0	26.0	41.0
47	34.91534654036529	38.0	34.0	40.0	25.0	41.0
48	34.80861726144462	37.0	34.0	40.0	25.0	41.0
49	34.63910650621315	37.0	33.0	40.0	24.0	41.0
50	34.47678769907658	37.0	33.0	40.0	24.0	41.0
51	33.474018606761966	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	0.0
13	3.0
14	3.0
15	18.0
16	30.0
17	78.0
18	160.0
19	294.0
20	492.0
21	741.0
22	1029.0
23	1275.0
24	1677.0
25	1946.0
26	2283.0
27	2878.0
28	3518.0
29	4191.0
30	5292.0
31	6777.0
32	8855.0
33	11714.0
34	17086.0
35	27514.0
36	24789.0
37	32132.0
38	47368.0
39	57845.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.823239607250407	35.10362943391292	19.439412644752377	25.633718314084298
2	20.310138339236886	37.5681215939203	19.97592428070904	22.14581578613377
3	18.949821739682246	37.298904285554954	19.267113567398553	24.484160407364246
4	18.70752616215343	37.52850818997512	18.17909104544773	25.584874602423724
5	16.220727425167205	38.707295404460545	17.62027283251222	27.45170433786003
6	18.06448139131505	42.73055577990331	18.826750970143802	20.378211858637837
7	78.20147454165753	12.217081453619627	5.466265148281047	4.115178856441793
8	79.43987416013815	12.31284589616673	4.384396164807145	3.8628837788879786
9	71.4779645633103	17.077992254233443	7.939603019849008	3.5044401626072545
10	24.69414990788922	49.48752562371882	12.66705895474457	13.151265513647395
11	17.76218881363624	33.741389853584245	32.61683069692669	15.879590635852821
12	21.75429690053959	29.690823151150138	32.551833946764205	16.003046001546075
13	22.17042994004146	30.547703384061563	31.35227853991916	15.929588135977815
14	18.1375546607285	35.16016506866964	29.808509574521274	16.893770696080583
15	15.123474595500994	37.02661020795114	30.642698634299055	17.20721656224881
16	17.443358601300705	35.087860991565805	28.598570071496425	18.870210335637065
17	16.267648156053735	34.39981847061493	29.89619749781742	19.436335875513915
18	16.715702676404643	33.53678469922658	30.86076465407499	18.886747970293793
19	16.305723175379693	38.489998576994225	29.512755131474194	15.691523116151885
20	17.039917234907488	36.05088976320415	29.626595593297257	17.28259740859111
21	18.35062093049194	35.86128385888398	28.252433532169547	17.535661678454538
22	16.158422848088364	33.755235315157314	29.025856399487722	21.0604854372666
23	15.5111475195471	37.55389153619242	28.151669339609942	18.783291604650536
24	14.319668631953016	37.93694930638083	28.39550330175799	19.34787875990816
25	16.022660405441265	37.180833266029005	25.813324718379466	20.98318161015026
26	17.76372719825547	35.484764223327296	26.921346240380288	19.830162338036946
27	14.597347055724136	36.54394203366755	28.3105075515455	20.548203359062818
28	15.41961363470288	36.10088726332914	28.883940418363697	19.59555868360428
29	17.643348601800678	36.848542188275204	26.91865406729664	18.589455142627482
30	17.821801217631425	36.66162845703869	27.16979535638603	18.346774968943862
31	17.981793218031406	36.16819159042048	24.7633772157546	21.08663797579352
32	18.512920507820763	35.72898278163015	26.945960394287976	18.81213631626111
33	16.27957063685277	35.948587186025314	27.16441101021872	20.607431166903194
34	16.196113271259513	33.88445962317269	28.257433282182042	21.661993823385753
35	17.36374719725552	34.364051028217816	28.014753108498418	20.25744866602824
36	18.45638487306404	36.30741539846085	27.733228723179227	17.50297100529589
37	16.50071342586717	37.13122036205882	25.514493506093928	20.853572705980085
38	17.414129293535325	34.990173568244664	28.792406533519475	18.803290604700535
39	16.70608777253445	33.67293173802848	28.62587639848777	20.995104090949297
40	18.0417902181814	34.481737451588955	27.45170433786003	20.024767992369615
41	18.368696949767894	32.40030306176999	29.89888967090107	19.332110317561042
42	19.527869760358136	33.56947537238523	26.042159430490013	20.860495436766623
43	17.548737947718	34.19482872010246	27.1482579717168	21.10817536046275
44	17.227600158453615	33.247952986966034	27.731305742405187	21.79314111217516
45	18.10678696834389	33.78677219985155	28.848557572121393	19.25788325968317
46	17.076069273459403	34.20059766242457	30.645006211227898	18.078326852888125
47	16.800313830462326	34.72749439451104	28.17936026275609	20.29283151227054
48	17.747958755908357	33.95137935410922	28.88817097606658	19.412490913915843
49	17.53027733228723	33.544476622322726	29.04200943798964	19.8832366074004
50	17.190294331437276	32.154161522693094	28.701641840984877	21.953902304884757
51	17.98102402572179	30.49962886471061	30.60577740343752	20.91356970613008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12531.0
1	9269.0
2	6007.0
3	3694.5
4	1382.0
5	1146.5
6	911.0
7	956.5
8	1002.0
9	1167.0
10	1332.0
11	1632.5
12	1933.0
13	2136.0
14	2339.0
15	2335.0
16	2331.0
17	2165.0
18	1999.0
19	1977.0
20	1955.0
21	1943.5
22	1932.0
23	2515.0
24	3098.0
25	2944.0
26	3740.0
27	4690.0
28	5744.5
29	6799.0
30	7191.5
31	7584.0
32	9270.0
33	10956.0
34	9329.5
35	7703.0
36	9329.5
37	10956.0
38	11579.5
39	12203.0
40	12992.5
41	13782.0
42	13864.0
43	13946.0
44	15101.0
45	16256.0
46	18099.0
47	19942.0
48	21968.0
49	23994.0
50	21734.5
51	19475.0
52	17324.5
53	15174.0
54	12382.0
55	9590.0
56	8196.0
57	6802.0
58	5969.0
59	5136.0
60	4471.5
61	3807.0
62	3485.0
63	3163.0
64	2694.5
65	2226.0
66	1915.5
67	1605.0
68	1255.0
69	905.0
70	743.5
71	582.0
72	562.5
73	543.0
74	445.5
75	243.0
76	138.0
77	99.5
78	61.0
79	47.0
80	33.0
81	24.0
82	15.0
83	16.5
84	18.0
85	14.5
86	11.0
87	8.0
88	5.0
89	8.5
90	12.0
91	10.5
92	9.0
93	5.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	260013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.234642114048143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.37952527539625	23.093076115425
2	9.531381178924873	5.763557975947356
3	3.7016307527921235	3.357524431470734
4	1.9500343450276032	2.3583436212804747
5	1.196987813875391	1.8095249083699663
6	0.8815223751494645	1.5991508116901847
7	0.6894446281832752	1.4591578113402024
8	0.48464650062329867	1.1722490798536995
9	0.4503014730200728	1.2253233492171545
>10	3.849187167680057	24.74607038878825
>50	0.5775052789579465	12.28169360762731
>100	0.30147302007276056	14.213904689380916
>500	0.0	0.0
>1k	0.005088152237514946	2.6814043913188956
>5k	0.0	0.0
>10k+	0.0012720380593787366	4.239018818289854
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11022	4.239018818289854	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2411	0.9272613292412302	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1737	0.668043520900878	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1662	0.6391988092903047	RNA PCR Primer, Index 38 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1162	0.44690073188648255	No Hit
AAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	473	0.18191398122401573	RNA PCR Primer, Index 27 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	415	0.15960740424517236	No Hit
ATTAATGGGAACATTTATCTATTAAAATTGCCATTTCATTAAGCAATTTTC	349	0.13422405802786783	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	345	0.13268567340863727	No Hit
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	345	0.13268567340863727	No Hit
ACCAAGGGATTGTCTTTTAAGGTAATCTTTTCTGAACATTTATCTATCATG	318	0.12230157722883087	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	298	0.11460965413267797	No Hit
AATACTGGGATCACTTCTGTGTTAAAATGGAAGGTTTTTTGTGATGTATGA	279	0.10730232719133273	No Hit
GGCTGAGGGATATATTTTCTTTTTATTCATAAATTACTACACATTGTGAGT	279	0.10730232719133273	No Hit
AACTATGGGTGGTGATGAAGACAGGTATGTACATTTTTTTAAATTTTGAAT	277	0.10653313488171745	No Hit
CTCGATGGGGTCTTCCTTTGCCTTTCCTCTTGATAGTGTTTTCTGATGAGC	273	0.10499475026248686	No Hit
GCAGCAGGGAGAAGCTATTTTTTACTTTTTTTACTTGGAAATAAATCCTCT	267	0.10268717333364102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7580390211258667	0.0
2	0.0	0.0	0.0	0.9991808101902597	0.0
3	0.0	0.0	0.0	1.2595524069950348	0.0
4	0.0	0.0	0.0	2.5506416986842964	0.0
5	0.0	0.0	0.0	2.714864256787161	0.0
6	0.0	0.0	0.0	3.7367362401110715	0.0
7	0.0	0.0	0.0	4.4678535304004035	0.0
8	0.0	0.0	0.0	5.053208878017638	0.0
9	0.0	0.0	0.0	5.941241399468488	0.0
10	0.0	0.0	0.0	7.4153984608461885	0.0
11	0.0	0.0	0.0	8.687642540949875	0.0
12	0.0	0.0	0.0	9.381838600377673	0.0
13	0.0	0.0	0.0	9.64374858180168	0.0
14	0.0	0.0	0.0	9.93258029406222	0.0
15	0.0	0.0	0.0	10.180644813913151	0.0
16	0.0	0.0	0.0	10.744847373015965	0.0
17	0.0	0.0	0.0	11.48827174025914	0.0
18	0.0	0.0	0.0	12.57244830066189	0.0
19	0.0	0.0	0.0	12.93319949387146	0.0
20	0.0	0.0	0.0	13.375100475745443	0.0
21	0.0	0.0	0.0	13.825462573025195	0.0
22	0.0	0.0	0.0	14.29813124728379	0.0
23	0.0	0.0	0.0	14.849257537123144	0.0
24	0.0	0.0	0.0	15.198855441843293	0.0
25	0.0	0.0	0.0	15.469995730982681	0.0
26	0.0	0.0	0.0	15.774595885590335	0.0
27	0.0	0.0	0.0	16.112655905666255	0.0
28	0.0	0.0	0.0	16.45763865652871	0.0
29	0.0	0.0	0.0	16.796852465069055	0.0
30	0.0	0.0	0.0	17.23029233153727	0.0
31	0.0	0.0	0.0	17.582967005495878	0.0
32	0.0	0.0	0.0	17.92218081403622	0.0
33	0.0	0.0	0.0	18.307546161153482	0.0
34	0.0	0.0	0.0	18.67829685438805	0.0
35	0.0	0.0	0.0	19.095583682354345	0.0
36	0.0	0.0	0.0	19.43710506782353	0.0
37	0.0	0.0	0.0	19.81631687646387	0.0
38	0.0	0.0	0.0	20.241295627526316	0.0
39	0.0	0.0	0.0	20.64050643621665	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAT	20	7.023207E-4	45.000004	22
AACGTAG	40	6.7793735E-9	45.000004	1
CGGAAGA	20	7.023207E-4	45.000004	19
AGGTAAG	20	7.023207E-4	45.000004	18
TGGAGGT	20	7.023207E-4	45.000004	15
TTGGCGA	20	7.023207E-4	45.000004	13
TCGTTCA	40	6.7793735E-9	45.000004	16
ATCTCGC	20	7.023207E-4	45.000004	19
TCCGCTT	20	7.023207E-4	45.000004	19
TCACCGG	20	7.023207E-4	45.000004	2
GGTCGTT	20	7.023207E-4	45.000004	8
CGTGCTT	20	7.023207E-4	45.000004	11
CCTAGTT	20	7.023207E-4	45.000004	29
TTAGCGG	20	7.023207E-4	45.000004	2
TAGGTTC	20	7.023207E-4	45.000004	10
GACGTGC	20	7.023207E-4	45.000004	9
CGTTGGG	40	6.7793735E-9	45.000004	3
GGCGTAA	20	7.023207E-4	45.000004	36
ATTACGG	20	7.023207E-4	45.000004	2
GCTATAA	20	7.023207E-4	45.000004	16
>>END_MODULE