SRR3554127_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254924
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10524	4.1282892156093585	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2265	0.8885001019911817	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1726	0.6770645368815804	RNA PCR Primer, Index 38 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1647	0.6460749086002103	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1113	0.4366007123691767	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	444	0.17416955641681442	No Hit
AAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	418	0.1639704382482622	RNA PCR Primer, Index 27 (95% over 21bp)
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	389	0.1525944987525694	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	328	0.12866579843404308	No Hit
ATTAATGGGAACATTTATCTATTAAAATTGCCATTTCATTAAGCAATTTTC	328	0.12866579843404308	No Hit
ACCAAGGGATTGTCTTTTAAGGTAATCTTTTCTGAACATTTATCTATCATG	288	0.11297484740550126	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	286	0.11219029985407415	RNA PCR Primer, Index 38 (95% over 23bp)
GCACTAGGGGCATTCTGAATTTTAAGGTTTTTTTTTTTGTTTGGTTTGGTT	285	0.1117980260783606	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	284	0.11140575230264706	No Hit
AACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	283	0.11101347852693352	RNA PCR Primer, Index 38 (95% over 21bp)
CGAGGTAGTCTTCAAAGTTATCCATCCAAGCTTCCTCTTGCAGCAAAGTGA	279	0.10944438342407933	No Hit
AATACTGGGATCACTTCTGTGTTAAAATGGAAGGTTTTTTGTGATGTATGA	276	0.10826756209693869	No Hit
CTCGATGGGGTCTTCCTTTGCCTTTCCTCTTGATAGTGTTTTCTGATGAGC	268	0.10512937189123032	No Hit
GCAGCAGGGAGAAGCTATTTTTTACTTTTTTTACTTGGAAATAAATCCTCT	262	0.10277572923694907	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	40	6.7793735E-9	45.0	35
GGCAGTA	20	7.0229487E-4	45.0	21
CGGAACG	20	7.0229487E-4	45.0	1
TTGGCAT	25	3.882239E-5	45.0	13
TCGCAGA	20	7.0229487E-4	45.0	27
CAAACGG	20	7.0229487E-4	45.0	2
CAAACCG	20	7.0229487E-4	45.0	1
ACTGCGG	40	6.7793735E-9	45.0	2
CGCGGGC	45	3.8198777E-10	45.0	4
CATGCGG	25	3.882239E-5	45.0	2
CCGATGT	20	7.0229487E-4	45.0	12
GACACTC	20	7.0229487E-4	45.0	18
CGTGCGG	35	1.206954E-7	45.0	2
TTAGCGG	20	7.0229487E-4	45.0	2
TTAGCAC	20	7.0229487E-4	45.0	15
CAGCCGC	25	3.882239E-5	45.0	35
CCCTAGC	45	3.8198777E-10	45.0	21
AGCCCGG	20	7.0229487E-4	45.0	2
AGCCCGA	20	7.0229487E-4	45.0	15
ATCAGAG	25	3.882239E-5	45.0	27

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content