##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254924
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06604321287913	33.0	31.0	34.0	30.0	34.0
2	32.25380897836218	34.0	31.0	34.0	30.0	34.0
3	32.356729848896144	34.0	31.0	34.0	30.0	34.0
4	35.91044781974235	37.0	35.0	37.0	35.0	37.0
5	35.7344698812195	37.0	35.0	37.0	35.0	37.0
6	35.69666253471623	37.0	35.0	37.0	33.0	37.0
7	35.615665845506896	37.0	35.0	37.0	35.0	37.0
8	35.78718755393764	37.0	35.0	37.0	35.0	37.0
9	37.4670450801023	39.0	37.0	39.0	35.0	39.0
10	37.007704256955016	39.0	37.0	39.0	33.0	39.0
11	37.069357926283914	39.0	37.0	39.0	33.0	39.0
12	37.08882647377258	39.0	37.0	39.0	33.0	39.0
13	37.04553906262259	39.0	37.0	39.0	33.0	39.0
14	38.190672514161086	40.0	38.0	41.0	33.0	41.0
15	38.1985140669376	40.0	37.0	41.0	33.0	41.0
16	38.22516122452182	40.0	37.0	41.0	33.0	41.0
17	38.13528737976809	40.0	37.0	41.0	33.0	41.0
18	37.90627402676876	39.0	37.0	41.0	33.0	41.0
19	37.73920854843012	39.0	36.0	41.0	33.0	41.0
20	37.646996751973134	39.0	36.0	41.0	33.0	41.0
21	37.5914782444964	39.0	35.0	41.0	33.0	41.0
22	37.53848990287301	39.0	35.0	41.0	33.0	41.0
23	37.37373099433557	39.0	35.0	41.0	32.0	41.0
24	37.31995025968524	39.0	35.0	41.0	32.0	41.0
25	37.242401656964425	39.0	35.0	41.0	32.0	41.0
26	37.18644772559665	39.0	35.0	41.0	32.0	41.0
27	37.119364987211874	39.0	35.0	41.0	32.0	41.0
28	37.006660808711615	39.0	35.0	41.0	32.0	41.0
29	36.90948674899186	39.0	35.0	41.0	31.0	41.0
30	36.78162118906027	39.0	35.0	41.0	31.0	41.0
31	36.704296182391616	39.0	35.0	40.0	31.0	41.0
32	36.599837598656855	39.0	35.0	40.0	31.0	41.0
33	36.444214746355776	39.0	35.0	40.0	30.0	41.0
34	36.4002487015738	39.0	35.0	40.0	30.0	41.0
35	36.2580259214511	38.0	35.0	40.0	30.0	41.0
36	36.189252483093	38.0	35.0	40.0	30.0	41.0
37	36.09116050273807	38.0	35.0	40.0	30.0	41.0
38	36.026623621157675	38.0	35.0	40.0	30.0	41.0
39	35.93162275815537	38.0	35.0	40.0	30.0	41.0
40	35.820664197957036	38.0	35.0	40.0	29.0	41.0
41	35.758371122373724	38.0	35.0	40.0	29.0	41.0
42	35.641414696144736	38.0	35.0	40.0	28.0	41.0
43	35.54092984575795	38.0	35.0	40.0	28.0	41.0
44	35.48476408655129	38.0	35.0	40.0	27.0	41.0
45	35.372118749117384	38.0	34.0	40.0	27.0	41.0
46	35.19603097393733	38.0	34.0	40.0	26.0	41.0
47	35.09624437087132	38.0	34.0	40.0	26.0	41.0
48	34.97631843216017	38.0	34.0	40.0	26.0	41.0
49	34.844290847468265	37.0	34.0	40.0	26.0	41.0
50	34.724572813858245	37.0	34.0	40.0	25.0	41.0
51	33.74149550454253	36.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	1.0
12	1.0
13	1.0
14	6.0
15	19.0
16	29.0
17	73.0
18	130.0
19	256.0
20	413.0
21	646.0
22	888.0
23	1272.0
24	1597.0
25	1888.0
26	2222.0
27	2518.0
28	3177.0
29	3731.0
30	4891.0
31	6253.0
32	7923.0
33	10881.0
34	16219.0
35	26570.0
36	23892.0
37	31818.0
38	46932.0
39	60644.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.804333840674083	35.07986694073528	19.666253471622916	25.449545746967722
2	20.685380740926707	37.23031177919694	19.923585068491	22.160722411385354
3	19.080196450706875	37.31190472454535	19.20140904740236	24.406489777345406
4	18.83384851955877	37.32720340179818	18.044201409047403	25.794746669595643
5	16.26955484771932	38.68760885597276	17.565627402676874	27.477208893631044
6	18.095589273665876	42.577395615948284	18.83502534088591	20.49198976949993
7	78.15741162071834	12.060064960537257	5.586370839936609	4.196152578807801
8	79.42327909494594	12.232273148075505	4.385620812477444	3.9588269445011064
9	71.43148546233388	16.981139476863692	8.037297390594844	3.5500776702075916
10	24.772873483861858	49.22996657827431	12.778710517644473	13.218449420219361
11	17.784516169525038	33.69514051246646	32.645023614881296	15.875319703127206
12	21.866517079600197	29.59627183003562	32.53440241013008	16.00280868023411
13	22.032841160502738	30.547143462365256	31.30776231347382	16.112253063658187
14	18.196011360248544	35.07437510787529	29.873217115689382	16.856396416186787
15	15.039384287081639	36.960819695281735	30.7338657796049	17.265930238031725
16	17.18198365002903	34.93315654861841	28.775242817467166	19.109616983885395
17	16.25425617046649	34.221964193249754	30.099166810500382	19.42461282578337
18	16.649668136385745	33.36092325555852	30.86096248293609	19.128446125119645
19	16.181685521959484	38.46126688738604	29.752004518993896	15.605043071660575
20	16.98074720308798	36.046429524093455	29.854387974455133	17.118435298363433
21	18.366258178908225	35.851077183788114	28.310398393246615	17.472266244057053
22	16.167171392258084	33.600210258743786	29.193014388602094	21.03960396039604
23	15.581114371342048	37.455084652680796	28.220567698608214	18.743233277368944
24	14.278765435973073	37.96072554957556	28.57439864430183	19.186110370149535
25	16.07106431720827	37.07340226891152	25.830835856961293	21.024697556918927
26	17.83590403414351	35.37995637915614	26.83387990146083	19.950259685239523
27	14.54708069856114	36.54500949302537	28.357078972556526	20.550830835856964
28	15.531295601826425	35.97425114936216	28.903516342125496	19.59093690668591
29	17.778632062889333	36.58031413283959	27.072774630870377	18.5682791734007
30	17.935541573174753	36.555208611193926	27.19830223909871	18.310947576532612
31	18.021057256280304	36.06290502267343	24.769735293656147	21.146302427390122
32	18.371750011768214	35.60355243131286	27.277149268017137	18.74754828890179
33	16.340948674899185	36.03348448949491	27.049630478103275	20.575936357502627
34	16.326434545197785	33.844596821013326	28.246850041581016	21.582118592207873
35	17.366352324614397	34.34670725392666	27.93106965213162	20.35587076932733
36	18.500808083977972	36.08918736564623	27.669423043730678	17.740581506645118
37	16.64299948219862	37.12557468108142	25.38285920509642	20.848566631623545
38	17.400872416877185	34.900597825234186	28.759159592662915	18.939370165225714
39	16.713608761827054	33.74417473443066	28.52104941080479	21.021167092937503
40	18.045378230374542	34.56441919944768	27.420329196152576	19.969873374025198
41	18.372926833095356	32.29354631184196	29.943434121542108	19.39009273352058
42	19.52071990083319	33.546076477695316	26.08071425209082	20.852489369380677
43	17.72292918673801	34.174106792612704	27.114747924871725	20.988216095777563
44	17.15020947419623	33.198521912413106	27.800442484819005	21.85082612857165
45	18.22974690495991	33.644929469175125	28.875664904049835	19.24965872181513
46	17.099606157129184	34.11879619023709	30.787607286877655	17.99399036575607
47	16.796770802278328	34.715444603097396	28.214683591972513	20.27310100265177
48	17.64486670537101	33.79830851547913	28.98079427594106	19.5760305032088
49	17.62682211168819	33.330718174828576	29.19576030503209	19.846699408451148
50	17.32830176837018	31.9993409800568	28.669721171800223	22.002636079772795
51	18.07636785865591	30.411024462192653	30.538513439299557	20.97409423985188
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11997.0
1	8909.0
2	5821.0
3	3585.0
4	1349.0
5	1111.0
6	873.0
7	960.0
8	1047.0
9	1206.5
10	1366.0
11	1630.5
12	1895.0
13	2103.5
14	2312.0
15	2268.5
16	2225.0
17	2117.0
18	2009.0
19	1978.0
20	1947.0
21	1955.5
22	1964.0
23	2556.5
24	3149.0
25	2978.0
26	3678.5
27	4550.0
28	5625.0
29	6700.0
30	7000.5
31	7301.0
32	9075.0
33	10849.0
34	9325.5
35	7802.0
36	9270.0
37	10738.0
38	11327.0
39	11916.0
40	12731.0
41	13546.0
42	13405.0
43	13264.0
44	14602.5
45	15941.0
46	17582.0
47	19223.0
48	21258.0
49	23293.0
50	21266.5
51	19240.0
52	17113.5
53	14987.0
54	12294.5
55	9602.0
56	8188.0
57	6774.0
58	5892.0
59	5010.0
60	4381.5
61	3753.0
62	3505.5
63	3258.0
64	2769.0
65	2280.0
66	1886.0
67	1492.0
68	1213.0
69	934.0
70	755.0
71	576.0
72	544.0
73	512.0
74	406.5
75	226.0
76	151.0
77	105.0
78	59.0
79	49.0
80	39.0
81	29.5
82	20.0
83	21.0
84	22.0
85	15.5
86	9.0
87	7.5
88	6.0
89	6.5
90	7.0
91	6.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	254924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.121134141940342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.87841533612898	22.855439268174045
2	9.667126819993229	5.823696474243304
3	3.7064048133774383	3.349233497042256
4	2.094131742765608	2.5231049253895277
5	1.3531112442372306	2.037862264831871
6	0.8829734587034096	1.5957697196027052
7	0.6928346313129998	1.4608275407572453
8	0.5039981246581408	1.2144796096091384
9	0.42325423905399423	1.147400793962122
>10	3.9056598859167035	24.715209238831964
>50	0.5808350480556351	12.318965652508199
>100	0.3047430521188758	14.181481539596113
>500	0.0	0.0
>1k	0.005209282942203006	2.648240259842149
>5k	0.0	0.0
>10k+	0.0013023207355507514	4.1282892156093585
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10524	4.1282892156093585	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2265	0.8885001019911817	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1726	0.6770645368815804	RNA PCR Primer, Index 38 (95% over 24bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1647	0.6460749086002103	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1113	0.4366007123691767	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	444	0.17416955641681442	No Hit
AAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	418	0.1639704382482622	RNA PCR Primer, Index 27 (95% over 21bp)
AAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	389	0.1525944987525694	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	328	0.12866579843404308	No Hit
ATTAATGGGAACATTTATCTATTAAAATTGCCATTTCATTAAGCAATTTTC	328	0.12866579843404308	No Hit
ACCAAGGGATTGTCTTTTAAGGTAATCTTTTCTGAACATTTATCTATCATG	288	0.11297484740550126	No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	286	0.11219029985407415	RNA PCR Primer, Index 38 (95% over 23bp)
GCACTAGGGGCATTCTGAATTTTAAGGTTTTTTTTTTTGTTTGGTTTGGTT	285	0.1117980260783606	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	284	0.11140575230264706	No Hit
AACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	283	0.11101347852693352	RNA PCR Primer, Index 38 (95% over 21bp)
CGAGGTAGTCTTCAAAGTTATCCATCCAAGCTTCCTCTTGCAGCAAAGTGA	279	0.10944438342407933	No Hit
AATACTGGGATCACTTCTGTGTTAAAATGGAAGGTTTTTTGTGATGTATGA	276	0.10826756209693869	No Hit
CTCGATGGGGTCTTCCTTTGCCTTTCCTCTTGATAGTGTTTTCTGATGAGC	268	0.10512937189123032	No Hit
GCAGCAGGGAGAAGCTATTTTTTACTTTTTTTACTTGGAAATAAATCCTCT	262	0.10277572923694907	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9227377571354597E-4	0.0	0.0	0.7665029577442689	0.0
2	3.9227377571354597E-4	0.0	0.0	1.0199118168552197	0.0
3	3.9227377571354597E-4	0.0	0.0	1.2803816039290141	0.0
4	3.9227377571354597E-4	0.0	0.0	2.616466084009352	0.0
5	3.9227377571354597E-4	0.0	0.0	2.7792597009304734	0.0
6	3.9227377571354597E-4	0.0	0.0	3.8101551835056724	0.0
7	3.9227377571354597E-4	0.0	0.0	4.561751737772826	0.0
8	3.9227377571354597E-4	0.0	0.0	5.150946948894573	0.0
9	3.9227377571354597E-4	0.0	0.0	6.0837739875413845	0.0
10	3.9227377571354597E-4	0.0	0.0	7.578729346785709	0.0
11	3.9227377571354597E-4	0.0	0.0	8.89520013808037	0.0
12	3.9227377571354597E-4	0.0	0.0	9.652680798983226	0.0
13	3.9227377571354597E-4	0.0	0.0	9.906089658094178	0.0
14	3.9227377571354597E-4	0.0	0.0	10.174797194457955	0.0
15	3.9227377571354597E-4	0.0	0.0	10.423498768260345	0.0
16	3.9227377571354597E-4	0.0	0.0	10.98719618396071	0.0
17	3.9227377571354597E-4	0.0	0.0	11.736831369349296	0.0
18	3.9227377571354597E-4	0.0	0.0	12.819899264094397	0.0
19	3.9227377571354597E-4	0.0	0.0	13.187852065713702	0.0
20	3.9227377571354597E-4	0.0	0.0	13.641712824214276	0.0
21	3.9227377571354597E-4	0.0	0.0	14.080667179237734	0.0
22	3.9227377571354597E-4	0.0	0.0	14.562379375813968	0.0
23	3.9227377571354597E-4	0.0	0.0	15.080180759755848	0.0
24	3.9227377571354597E-4	0.0	0.0	15.461863143525129	0.0
25	3.9227377571354597E-4	0.0	0.0	15.745477083366023	0.0
26	3.9227377571354597E-4	0.0	0.0	16.06243429414257	0.0
27	3.9227377571354597E-4	0.0	0.0	16.427248905556166	0.0
28	3.9227377571354597E-4	0.0	0.0	16.763035257566962	0.0
29	3.9227377571354597E-4	0.0	0.0	17.143933093784813	0.0
30	3.9227377571354597E-4	0.0	0.0	17.563273760022597	0.0
31	3.9227377571354597E-4	0.0	0.0	17.948486607773297	0.0
32	3.9227377571354597E-4	0.0	0.0	18.28388068600838	0.0
33	3.9227377571354597E-4	0.0	0.0	18.65340258273054	0.0
34	3.9227377571354597E-4	0.0	0.0	19.014686730162715	0.0
35	3.9227377571354597E-4	0.0	0.0	19.453641085186174	0.0
36	3.9227377571354597E-4	0.0	0.0	19.843168944469724	0.0
37	3.9227377571354597E-4	0.0	0.0	20.233873625080417	0.0
38	3.9227377571354597E-4	0.0	0.0	20.65439111264534	0.0
39	3.9227377571354597E-4	0.0	0.0	21.06392493449028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	40	6.7793735E-9	45.0	35
GGCAGTA	20	7.0229487E-4	45.0	21
CGGAACG	20	7.0229487E-4	45.0	1
TTGGCAT	25	3.882239E-5	45.0	13
TCGCAGA	20	7.0229487E-4	45.0	27
CAAACGG	20	7.0229487E-4	45.0	2
CAAACCG	20	7.0229487E-4	45.0	1
ACTGCGG	40	6.7793735E-9	45.0	2
CGCGGGC	45	3.8198777E-10	45.0	4
CATGCGG	25	3.882239E-5	45.0	2
CCGATGT	20	7.0229487E-4	45.0	12
GACACTC	20	7.0229487E-4	45.0	18
CGTGCGG	35	1.206954E-7	45.0	2
TTAGCGG	20	7.0229487E-4	45.0	2
TTAGCAC	20	7.0229487E-4	45.0	15
CAGCCGC	25	3.882239E-5	45.0	35
CCCTAGC	45	3.8198777E-10	45.0	21
AGCCCGG	20	7.0229487E-4	45.0	2
AGCCCGA	20	7.0229487E-4	45.0	15
ATCAGAG	25	3.882239E-5	45.0	27
>>END_MODULE