##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554126_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197072
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.321572136011866	34.0	31.0	34.0	30.0	34.0
2	32.50907631287007	34.0	31.0	34.0	31.0	34.0
3	32.59877434272959	34.0	31.0	34.0	31.0	34.0
4	36.10823743266904	37.0	35.0	37.0	35.0	37.0
5	35.94762637502172	37.0	35.0	37.0	35.0	37.0
6	35.919800981060455	37.0	35.0	37.0	35.0	37.0
7	35.99858822192817	37.0	35.0	37.0	35.0	37.0
8	36.09395424836601	37.0	35.0	37.0	35.0	37.0
9	37.73058512771161	39.0	38.0	39.0	35.0	39.0
10	37.30804245692824	39.0	37.0	39.0	34.0	39.0
11	37.2371703623508	39.0	37.0	39.0	34.0	39.0
12	37.1865050723766	39.0	37.0	39.0	34.0	39.0
13	37.16918614753332	39.0	37.0	39.0	33.0	39.0
14	38.425803126294824	40.0	38.0	41.0	34.0	41.0
15	38.441229934373204	40.0	38.0	41.0	34.0	41.0
16	38.42156194447786	40.0	38.0	41.0	34.0	41.0
17	38.391821043345765	40.0	38.0	41.0	34.0	41.0
18	38.31856981033722	40.0	38.0	41.0	34.0	41.0
19	38.31894823369021	40.0	38.0	41.0	34.0	41.0
20	38.249484575698034	40.0	38.0	41.0	34.0	41.0
21	38.216413047836724	40.0	37.0	41.0	34.0	41.0
22	38.15764715906813	40.0	37.0	41.0	34.0	41.0
23	38.09907089966184	40.0	37.0	41.0	33.0	41.0
24	38.01612852025609	40.0	37.0	41.0	33.0	41.0
25	37.945927229105685	40.0	37.0	41.0	33.0	41.0
26	37.86095823810096	40.0	37.0	41.0	33.0	41.0
27	37.78574805859631	40.0	37.0	41.0	33.0	41.0
28	37.71929090313699	40.0	37.0	41.0	33.0	41.0
29	37.60688245986875	40.0	36.0	41.0	33.0	41.0
30	37.55540936551853	40.0	36.0	41.0	33.0	41.0
31	37.51643593701966	40.0	36.0	41.0	32.0	41.0
32	37.42905522808987	40.0	36.0	41.0	32.0	41.0
33	37.340567651736905	40.0	36.0	41.0	32.0	41.0
34	37.22684182739217	39.0	36.0	41.0	31.0	41.0
35	37.191320154510336	39.0	36.0	41.0	31.0	41.0
36	37.075998770332944	39.0	35.0	41.0	31.0	41.0
37	37.00073429167168	39.0	35.0	41.0	31.0	41.0
38	36.959383395484984	39.0	35.0	41.0	31.0	41.0
39	36.87514619003702	39.0	35.0	41.0	31.0	41.0
40	36.79767800098908	39.0	35.0	41.0	31.0	41.0
41	36.71309829316866	39.0	35.0	41.0	31.0	41.0
42	36.611076860873865	39.0	35.0	41.0	30.0	41.0
43	36.52725316438777	39.0	35.0	41.0	30.0	41.0
44	36.426915841319484	39.0	35.0	41.0	30.0	41.0
45	36.306706697675665	39.0	35.0	40.0	30.0	41.0
46	36.199202721306655	39.0	35.0	40.0	30.0	41.0
47	36.05492317922398	38.0	35.0	40.0	29.0	41.0
48	35.963760742879295	38.0	35.0	40.0	29.0	41.0
49	35.82187537591724	38.0	34.0	40.0	29.0	41.0
50	35.682315683601324	38.0	34.0	40.0	28.0	41.0
51	34.625351691460494	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	3.0
10	6.0
11	11.0
12	15.0
13	26.0
14	38.0
15	55.0
16	113.0
17	205.0
18	336.0
19	614.0
20	982.0
21	1523.0
22	2319.0
23	3141.0
24	4330.0
25	5732.0
26	7458.0
27	9261.0
28	11995.0
29	14969.0
30	19158.0
31	24820.0
32	32126.0
33	43104.0
34	66374.0
35	82612.0
36	99482.0
37	146502.0
38	246427.0
39	373183.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.7088863493591	31.748382720504697	19.52848283144205	27.01424809869415
2	24.347324137562318	29.622696045016504	19.967303554005106	26.06267626341607
3	21.439813144071536	30.402348396754746	20.635851477605357	27.521986981568357
4	21.621339401472927	31.515982330219067	18.99117179250705	27.871506475800956
5	19.485711803467126	33.952844941657645	16.65505500086879	29.906388254006444
6	21.195049253511904	37.44929294144379	18.91749201384712	22.438165791197186
7	86.02615381530936	5.568086130157584	4.423125760188192	3.982634294344868
8	87.22382613577129	5.278295708194661	4.07552762072791	3.422350535306147
9	78.51081639199647	9.61938797332157	6.839939452263523	5.029856182418435
10	33.94967052942513	36.754096662523224	14.529368325380595	14.76686448267105
11	24.57763609874761	28.654917999919803	28.51240359811273	18.255042303219856
12	26.283882673723884	24.60486921421602	30.911674485745216	18.199573626314873
13	24.589331301709507	25.82342582568133	29.08738989801783	20.499852974591338
14	21.52284908510098	27.631253592098055	29.092485665022654	21.75341165777831
15	21.2913676036195	29.092736276514696	29.414437895130785	20.20145822473502
16	22.58744670328936	28.206072817675125	28.4023016159429	20.804178863092613
17	22.248202280230426	27.29668725022388	28.87378536963524	21.581325099910448
18	22.476676423807422	27.297773233356054	29.249117847548018	20.976432495288506
19	21.957242337971316	28.827338706443722	28.157203576727213	21.058215378857746
20	23.117322934627158	27.163194862130265	29.291805338358927	20.427676864883647
21	23.643189382092306	28.134230856623493	27.819212211128487	20.403367550155714
22	22.624286592619324	27.386406164374407	27.536188299450657	22.45311894355561
23	21.99182672387292	28.15870724567946	28.731103893500144	21.118362136947486
24	21.00909552641779	27.942847213868504	30.267185265380864	20.780871994332838
25	21.71022294398332	29.56714383094751	26.89838205220739	21.824251172861782
26	22.664384431345816	28.173075637889784	27.151332584840343	22.011207345924056
27	20.735010091289414	29.101507678736116	27.71270232701124	22.45077990296323
28	21.44165096167983	28.485086945480305	28.89692516406699	21.176336928772873
29	21.913385326864216	28.70712872742826	26.69054158814173	22.688944357565795
30	22.320461927102127	28.709300693692608	28.405141879519363	20.5650954996859
31	22.999117847548018	28.20398438857479	27.030871994332838	21.766025769544356
32	23.14088041487897	28.419259660237646	27.733085395030542	20.706774529852844
33	21.853656254594544	28.736450271997004	27.6513025114613	21.758590961947153
34	21.285102316318483	28.768361468650173	28.679227314647743	21.267308900383604
35	22.54835131053103	27.82489273828141	29.085802691901574	20.540953259285992
36	22.32472232246682	30.64995255089084	27.201204271756417	19.824120854885923
37	20.760321851985513	30.7645655399174	28.422517609634173	20.052594998462915
38	20.901332584840343	29.084633171605383	29.957429461218705	20.056604782335565
39	20.97442760335218	28.746224120186586	28.781810952056354	21.49753732440488
40	22.922347193819586	28.248175548337944	28.8426260074582	19.986851250384273
41	20.849957228972023	27.631002980606013	30.288988465188392	21.23005132523357
42	21.19880842589251	28.51549447318123	27.954625953994412	22.331071146931848
43	21.098730903404306	28.45576540091156	29.253461780076723	21.192041915607415
44	21.447164414504723	28.18744403010011	28.38668016627237	21.978711389122793
45	21.0603873451221	29.223889624015932	28.658008874988305	21.057714155873665
46	20.364773380381465	29.420285496611733	28.91263015090153	21.30231097210527
47	20.49091449804189	28.80052327679538	29.851838485905606	20.85672373925712
48	20.593331061122473	28.4998730235107	29.459046740714008	21.44774917465282
49	20.642033227742356	29.30508774743708	29.022815670235374	21.030063354585188
50	20.23729566809682	28.246755416549714	30.758467326944412	20.757481588409053
51	20.933828541641606	27.320077656147667	30.262423647032094	21.483670155178636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4878.0
1	3874.0
2	2870.0
3	2033.0
4	1196.0
5	1254.0
6	1312.0
7	1456.5
8	1601.0
9	1779.5
10	1958.0
11	2117.5
12	2277.0
13	2688.5
14	3100.0
15	3181.5
16	3263.0
17	3052.0
18	2841.0
19	3654.5
20	4468.0
21	5047.0
22	5626.0
23	7139.0
24	8652.0
25	9813.5
26	13853.0
27	16731.0
28	21785.0
29	26839.0
30	30260.5
31	33682.0
32	38456.0
33	43230.0
34	48322.5
35	53415.0
36	57589.0
37	61763.0
38	64214.0
39	66665.0
40	66384.0
41	66103.0
42	67065.0
43	68027.0
44	71334.0
45	74641.0
46	78918.5
47	83196.0
48	84692.5
49	86189.0
50	83026.0
51	79863.0
52	75490.0
53	71117.0
54	64657.5
55	58198.0
56	53358.5
57	48519.0
58	45827.0
59	43135.0
60	41067.5
61	39000.0
62	35852.5
63	32705.0
64	29532.0
65	26359.0
66	22807.5
67	19256.0
68	16567.0
69	13878.0
70	11819.5
71	9761.0
72	8593.0
73	7425.0
74	6280.0
75	4269.5
76	3404.0
77	2617.5
78	1831.0
79	1406.0
80	981.0
81	702.0
82	423.0
83	338.0
84	253.0
85	231.5
86	210.0
87	135.5
88	61.0
89	43.5
90	26.0
91	22.5
92	19.0
93	14.5
94	10.0
95	6.5
96	3.0
97	1.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1197072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.094197579420598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71575681856093	16.286904958658067
2	8.138446038538003	3.5962486952982307
3	3.184977831068155	2.111085884570829
4	1.8102851250727925	1.5998718891377766
5	1.2268233961618307	1.3552839254927638
6	0.9333248932610665	1.2372638758500987
7	0.7261580359748602	1.1230715384498802
8	0.617064109992849	1.0906829092281067
9	0.5650865773890262	1.1236621041280526
>10	7.403914710368348	36.831275489665224
>50	1.255006770512663	19.408680044529515
>100	0.4174476576709797	12.890663674841955
>500	0.003805356952333413	0.6167837981661993
>1k	0.0019026784761667064	0.7285212119833856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3579	0.29897951000441075	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1635	0.13658326316211555	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1232	0.10291778606466445	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.50611492040579E-4	0.0	0.0	0.09907507652004223	0.0
2	2.50611492040579E-4	0.0	0.0	0.1484455404520363	0.0
3	2.50611492040579E-4	0.0	0.0	0.19038119678682652	0.0
4	2.50611492040579E-4	0.0	0.0	0.37959287327746366	0.0
5	3.3414865605410534E-4	0.0	0.0	0.42871272571741714	0.0
6	3.3414865605410534E-4	0.0	0.0	0.791514629028162	0.0
7	3.3414865605410534E-4	0.0	0.0	1.06910862504511	0.0
8	3.3414865605410534E-4	0.0	0.0	1.3942352673857545	0.0
9	3.3414865605410534E-4	0.0	0.0	1.7186936124142909	0.0
10	3.3414865605410534E-4	0.0	0.0	2.2529137762807916	0.0
11	3.3414865605410534E-4	0.0	0.0	2.639941457155459	0.0
12	3.3414865605410534E-4	0.0	0.0	2.843855674512477	0.0
13	3.3414865605410534E-4	0.0	0.0	2.9394221901439512	0.0
14	3.3414865605410534E-4	0.0	0.0	3.0289740299664514	0.0
15	3.3414865605410534E-4	0.0	0.0	3.1085849472713423	0.0
16	3.3414865605410534E-4	0.0	0.0	3.2583670823475948	0.0
17	3.3414865605410534E-4	0.0	0.0	3.4317902348396756	0.0
18	3.3414865605410534E-4	0.0	0.0	3.6466478206824653	0.0
19	3.3414865605410534E-4	0.0	0.0	3.7508186642073325	0.0
20	3.3414865605410534E-4	0.0	0.0	3.8714463290428647	0.0
21	3.3414865605410534E-4	0.0	0.0	3.999926487295668	0.0
22	3.3414865605410534E-4	0.0	0.0	4.14636713581138	0.0
23	3.3414865605410534E-4	0.0	0.0	4.3047535987810255	0.0
24	3.3414865605410534E-4	0.0	0.0	4.4308947164414505	0.0
25	3.3414865605410534E-4	0.0	0.0	4.54600892845209	0.0
26	3.3414865605410534E-4	0.0	0.0	4.655776761965863	0.0
27	3.3414865605410534E-4	0.0	0.0	4.777908095753639	0.0
28	4.176858200676317E-4	0.0	0.0	4.899120520737266	0.0
29	5.01222984081158E-4	0.0	0.0	5.032947057486934	0.0
30	5.01222984081158E-4	0.0	0.0	5.210296456687651	0.0
31	5.847601480946843E-4	0.0	0.0	5.343120547469158	0.0
32	5.847601480946843E-4	0.0	0.0	5.501423473274791	0.0
33	5.847601480946843E-4	0.0	0.0	5.650453773874921	0.0
34	5.847601480946843E-4	0.0	0.0	5.800987743427296	0.0
35	5.847601480946843E-4	0.0	0.0	5.967310236978227	0.0
36	5.847601480946843E-4	0.0	0.0	6.118428966678696	0.0
37	5.847601480946843E-4	0.0	0.0	6.277149578304396	0.0
38	5.847601480946843E-4	0.0	0.0	6.441383642754989	0.0
39	5.847601480946843E-4	0.0	0.0	6.622742825828355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTA	25	3.8909024E-5	45.000004	27
CGGTCTA	25	3.8909024E-5	45.000004	31
TCATACG	25	3.8909024E-5	45.000004	1
TACCGTA	25	3.8909024E-5	45.000004	13
CCGATAT	20	7.0334045E-4	45.0	30
CGACATA	20	7.0334045E-4	45.0	40
ATATCGT	20	7.0334045E-4	45.0	39
CGTACGT	20	7.0334045E-4	45.0	14
TCGTACT	20	7.0334045E-4	45.0	31
TCGTAAT	20	7.0334045E-4	45.0	37
CGTCGGA	20	7.0334045E-4	45.0	26
TATCCCG	20	7.0334045E-4	45.0	1
CGTATGG	60	3.6379788E-12	41.249996	2
ATGCGAA	50	1.0822987E-9	40.500004	15
CGTAATG	45	1.9284926E-8	40.0	1
ACTACGG	205	0.0	39.512196	2
ACGTTAG	40	3.45839E-7	39.375	1
ATTACCG	80	0.0	39.375	1
ATTCACG	80	0.0	39.375	1
CATACGA	440	0.0	39.374996	18
>>END_MODULE