FastQCFastQC Report
Sat 18 Jun 2016
SRR3554125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170700
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34450.294268386435466No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA16560.14145383104125736No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12190.10412573673870335No Hit
ACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA12130.10361322285811908No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG700.045.0000041
CACTACG351.2119563E-745.0000041
TAATGCG207.0333405E-445.01
TAATACG302.165536E-644.9999961
TATCGAT302.165536E-644.99999613
AATTGCG603.6379788E-1241.2499961
CTTACCG603.6379788E-1241.2499961
AGTAACG556.184564E-1140.9090921
ACTCACG900.040.01
ACTACGG2550.039.7058832
GTTACGG1600.039.3752
AAACGGG8400.039.107143
GCTACGA2650.039.0566029
TATCCCG750.039.01
AACGGGA14700.038.8775524
ACGGGAG11250.038.85
ACGGGAC8300.038.765065
TACCCGC356.248387E-638.5714318
TCGAACG356.248387E-638.571431
TACGGGA8000.038.5312544
Produced by FastQC (version 0.11.5)