##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1170700
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39162210643205	34.0	31.0	34.0	30.0	34.0
2	32.57055266080123	34.0	31.0	34.0	31.0	34.0
3	32.649941060903735	34.0	31.0	34.0	31.0	34.0
4	36.13813530366448	37.0	35.0	37.0	35.0	37.0
5	35.991099342273856	37.0	35.0	37.0	35.0	37.0
6	35.9722798325788	37.0	35.0	37.0	35.0	37.0
7	36.024742461775006	37.0	35.0	37.0	35.0	37.0
8	36.124768087469036	37.0	36.0	37.0	35.0	37.0
9	37.762466045955414	39.0	38.0	39.0	35.0	39.0
10	37.35664730503118	39.0	37.0	39.0	35.0	39.0
11	37.30989664303408	39.0	37.0	39.0	34.0	39.0
12	37.27325531733151	39.0	37.0	39.0	34.0	39.0
13	37.25651832237123	39.0	37.0	39.0	34.0	39.0
14	38.52751174510976	40.0	38.0	41.0	34.0	41.0
15	38.53930554369181	40.0	38.0	41.0	34.0	41.0
16	38.52243187836337	40.0	38.0	41.0	34.0	41.0
17	38.4877218758008	40.0	38.0	41.0	34.0	41.0
18	38.419364482788076	40.0	38.0	41.0	34.0	41.0
19	38.42474417015461	40.0	38.0	41.0	34.0	41.0
20	38.35736140770479	40.0	38.0	41.0	34.0	41.0
21	38.31232339625865	40.0	38.0	41.0	34.0	41.0
22	38.259820620141795	40.0	38.0	41.0	34.0	41.0
23	38.19188946783975	40.0	37.0	41.0	34.0	41.0
24	38.14896301358162	40.0	37.0	41.0	34.0	41.0
25	38.073366362005636	40.0	37.0	41.0	33.0	41.0
26	38.01222687281114	40.0	37.0	41.0	33.0	41.0
27	37.94581532416503	40.0	37.0	41.0	33.0	41.0
28	37.85025454856069	40.0	37.0	41.0	33.0	41.0
29	37.7687708208764	40.0	37.0	41.0	33.0	41.0
30	37.714081318869056	40.0	37.0	41.0	33.0	41.0
31	37.64214059964124	40.0	36.0	41.0	33.0	41.0
32	37.57256256940292	40.0	36.0	41.0	33.0	41.0
33	37.49876227897839	40.0	36.0	41.0	33.0	41.0
34	37.38705560775605	40.0	36.0	41.0	32.0	41.0
35	37.31534210301529	40.0	36.0	41.0	32.0	41.0
36	37.243855812761595	40.0	36.0	41.0	32.0	41.0
37	37.16501323994191	39.0	35.0	41.0	31.0	41.0
38	37.12700606474759	39.0	35.0	41.0	31.0	41.0
39	37.059538737507474	39.0	35.0	41.0	31.0	41.0
40	36.95504057401555	39.0	35.0	41.0	31.0	41.0
41	36.87288203638848	39.0	35.0	41.0	31.0	41.0
42	36.79330827709917	39.0	35.0	41.0	31.0	41.0
43	36.704511830528745	39.0	35.0	41.0	31.0	41.0
44	36.61384470829418	39.0	35.0	41.0	31.0	41.0
45	36.486502092765015	39.0	35.0	41.0	30.0	41.0
46	36.34082343896814	39.0	35.0	40.0	30.0	41.0
47	36.243673870333986	39.0	35.0	40.0	30.0	41.0
48	36.146548219014264	38.0	35.0	40.0	30.0	41.0
49	36.03356709660887	38.0	35.0	40.0	29.0	41.0
50	35.91479627573247	38.0	35.0	40.0	29.0	41.0
51	34.90654992739387	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	11.0
11	17.0
12	14.0
13	32.0
14	41.0
15	68.0
16	121.0
17	190.0
18	321.0
19	554.0
20	889.0
21	1389.0
22	2049.0
23	2895.0
24	3962.0
25	5279.0
26	6601.0
27	8421.0
28	10640.0
29	13493.0
30	17507.0
31	22877.0
32	29115.0
33	39786.0
34	62553.0
35	79386.0
36	94394.0
37	140728.0
38	238904.0
39	388259.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.708037926027163	31.743230545827284	19.51892030409157	27.029811224053983
2	24.411206970188776	29.540531306056206	19.808405227641583	26.239856496113433
3	21.41769881267618	30.392073118646962	20.570427949090288	27.619800119586575
4	21.682326813017852	31.36542239685658	18.958998889553257	27.993251900572307
5	19.506705389937647	33.94413598701632	16.65601776714786	29.89314085589818
6	21.214572478004612	37.364055693175025	18.936960792688133	22.48441103613223
7	85.96224481079696	5.5553087896130515	4.407704792004783	4.074741607585206
8	87.10950713248484	5.240966942854702	4.120099086016913	3.5294268386435466
9	78.30374989322627	9.678995472794055	6.959511403433844	5.057743230545827
10	33.89220124711711	36.70718373622619	14.573161356453404	14.827453660203297
11	24.576748953617493	28.526180917399845	28.638506876227897	18.25856325275476
12	26.35722217476723	24.578286495259245	30.86221918510293	18.20227214487059
13	24.56590074314513	25.876654992739383	29.004612624925258	20.552831639190227
14	21.402835910139235	27.675066199709576	29.060818313829333	21.86127957632186
15	21.2442128640984	29.195609464422994	29.39958998889553	20.16058768258307
16	22.46356880498847	28.292474587853423	28.37063295464252	20.873323652515587
17	22.285811907405826	27.304433245067056	28.877423763560262	21.532331083966856
18	22.46613137439139	27.280088835739303	29.243700350217814	21.01007943965149
19	21.861450414282054	28.882378064405913	28.18151533270693	21.074656188605108
20	23.116426069872727	27.083198086614846	29.406338088323224	20.394037755189203
21	23.645682070556077	28.157170923379176	27.769795848637568	20.427351157427182
22	22.525924660459555	27.402494234218842	27.54044588707611	22.531135218245492
23	21.94763816520031	28.192534381139488	28.647475869138123	21.21235158452208
24	20.89143247629623	27.92765012385752	30.306312462629197	20.87460493721705
25	21.625779448193388	29.63756726744683	26.8404373451781	21.896215939181687
26	22.57828649525925	28.327496369693346	27.102673614077048	21.99154352097036
27	20.70069189373879	29.16870248569232	27.626975313914752	22.503630306654138
28	21.47492952934142	28.50328863073375	28.871956948834033	21.1498248910908
29	21.931664815922097	28.714444349534467	26.752455795677797	22.601435038865635
30	22.332108994618604	28.734688647817546	28.443239087725292	20.489963269838558
31	22.86614845818741	28.223199794994446	27.025881950969506	21.884769795848637
32	23.05398479542154	28.48022550610746	27.754505851200133	20.711283847270863
33	21.801144614333303	28.699496028017425	27.681387204236778	21.817972153412487
34	21.22960621850175	28.7524557956778	28.708037926027163	21.309900059793286
35	22.49278209618177	27.829759972665926	29.214828734944902	20.462629196207395
36	22.314854360638932	30.74092423336465	27.287264030067483	19.656957375928933
37	20.72853848125053	30.753139147518578	28.438455624839836	20.079866746391048
38	20.841718629879562	29.057486973605535	30.080891774152217	20.01990262236269
39	20.915520628683694	28.793884001025027	28.779362774408472	21.511232595882802
40	22.997181173656784	28.130861877509183	28.91048090885795	19.961476039976084
41	20.77970445032886	27.68343725975912	30.239514820193047	21.29734346971897
42	21.06389339711284	28.59904330742291	28.045101221491414	22.291962073972837
43	21.124626291962073	28.348253181857007	29.249081745963952	21.278038780216963
44	21.485863158793883	28.1431622106432	28.362432732553174	22.00854189800974
45	21.04698043905356	29.203126334671563	28.726317587768	21.023575638506877
46	20.27855129409755	29.376185188348853	29.028529939352527	21.316733578201077
47	20.495344665584692	28.648757153839583	29.929956436320147	20.925941744255574
48	20.600751687024857	28.44127445118305	29.531562313145983	21.42641154864611
49	20.64371743401384	29.3453489365337	28.949004868881868	21.0619287605706
50	20.21602460066627	28.285726488425727	30.705048261723753	20.79320064918425
51	20.963355257538225	27.35918681130947	30.210557785940033	21.466900145212268
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4681.0
1	3716.0
2	2751.0
3	1959.5
4	1168.0
5	1300.0
6	1432.0
7	1525.0
8	1618.0
9	1791.0
10	1964.0
11	2078.0
12	2192.0
13	2632.0
14	3072.0
15	3118.0
16	3164.0
17	2960.0
18	2756.0
19	3526.5
20	4297.0
21	4909.5
22	5522.0
23	6909.0
24	8296.0
25	9601.5
26	13568.0
27	16229.0
28	21260.0
29	26291.0
30	29844.0
31	33397.0
32	37771.5
33	42146.0
34	46891.5
35	51637.0
36	56089.5
37	60542.0
38	62773.5
39	65005.0
40	65099.0
41	65193.0
42	65859.5
43	66526.0
44	69569.0
45	72612.0
46	76920.0
47	81228.0
48	83279.0
49	85330.0
50	81629.0
51	77928.0
52	73712.5
53	69497.0
54	63316.5
55	57136.0
56	52304.0
57	47472.0
58	44776.5
59	42081.0
60	40016.5
61	37952.0
62	34855.0
63	31758.0
64	28814.5
65	25871.0
66	22382.5
67	18894.0
68	16243.0
69	13592.0
70	11564.5
71	9537.0
72	8367.5
73	7198.0
74	6039.5
75	4064.0
76	3247.0
77	2514.0
78	1781.0
79	1367.0
80	953.0
81	696.0
82	439.0
83	342.0
84	245.0
85	210.0
86	175.0
87	115.0
88	55.0
89	39.0
90	23.0
91	20.0
92	17.0
93	10.0
94	3.0
95	3.5
96	4.0
97	3.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1170700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.72990103965692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73894149968298	15.806100005175047
2	8.345325950885812	3.6268621411285897
3	3.3539907484945917	2.1864566115813693
4	1.9239434260875672	1.672284010191253
5	1.2946012645254228	1.4065778681976073
6	0.9920670052114331	1.293451070877195
7	0.7556076718415735	1.1493495953746067
8	0.6920123587981275	1.2029888059922293
9	0.5819038649773615	1.138024206049675
>10	7.593700764901006	36.895566272243215
>50	1.3195705708788306	19.998040131709942
>100	0.40279544713388077	12.303999270332008
>500	0.003561059945191472	0.5809629574968781
>1k	0.0019783666362174846	0.7393370536503855
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3445	0.294268386435466	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1656	0.14145383104125736	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1219	0.10412573673870335	No Hit
ACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	1213	0.10361322285811908	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7083796019475527E-4	0.0	0.0	0.10070897753480823	0.0
2	1.7083796019475527E-4	0.0	0.0	0.15341248825489023	0.0
3	1.7083796019475527E-4	0.0	0.0	0.20073460322883746	0.0
4	1.7083796019475527E-4	0.0	0.0	0.4083881438455625	0.0
5	1.7083796019475527E-4	0.0	0.0	0.45963953190398904	0.0
6	1.7083796019475527E-4	0.0	0.0	0.836678910053814	0.0
7	1.7083796019475527E-4	0.0	0.0	1.123942940121295	0.0
8	1.7083796019475527E-4	0.0	0.0	1.4591270180234048	0.0
9	1.7083796019475527E-4	0.0	0.0	1.7762022721448707	0.0
10	1.7083796019475527E-4	0.0	0.0	2.34560519347399	0.0
11	1.7083796019475527E-4	0.0	0.0	2.740411719484069	0.0
12	1.7083796019475527E-4	0.0	0.0	2.940975484752712	0.0
13	1.7083796019475527E-4	0.0	0.0	3.0414282053472284	0.0
14	1.7083796019475527E-4	0.0	0.0	3.133680703852396	0.0
15	1.7083796019475527E-4	0.0	0.0	3.2173058853677285	0.0
16	1.7083796019475527E-4	0.0	0.0	3.3708037926027163	0.0
17	1.7083796019475527E-4	0.0	0.0	3.5506961646877935	0.0
18	1.7083796019475527E-4	0.0	0.0	3.7627914922695824	0.0
19	1.7083796019475527E-4	0.0	0.0	3.873579909455881	0.0
20	1.7083796019475527E-4	0.0	0.0	3.9937644144528917	0.0
21	1.7083796019475527E-4	0.0	0.0	4.1282993081062616	0.0
22	1.7083796019475527E-4	0.0	0.0	4.28111386350047	0.0
23	1.7083796019475527E-4	0.0	0.0	4.441616127103442	0.0
24	1.7083796019475527E-4	0.0	0.0	4.578286495259246	0.0
25	1.7083796019475527E-4	0.0	0.0	4.694285470231486	0.0
26	1.7083796019475527E-4	0.0	0.0	4.8019988041342785	0.0
27	1.7083796019475527E-4	0.0	0.0	4.921841633210899	0.0
28	1.7083796019475527E-4	0.0	0.0	5.047749209874434	0.0
29	1.7083796019475527E-4	0.0	0.0	5.181002818826343	0.0
30	1.7083796019475527E-4	0.0	0.0	5.353976253523533	0.0
31	1.7083796019475527E-4	0.0	0.0	5.496967626206543	0.0
32	1.7083796019475527E-4	0.0	0.0	5.6544802255061075	0.0
33	1.7083796019475527E-4	0.0	0.0	5.809942769283335	0.0
34	1.7083796019475527E-4	0.0	0.0	5.960280174254719	0.0
35	1.7083796019475527E-4	0.0	0.0	6.127103442384898	0.0
36	1.7083796019475527E-4	0.0	0.0	6.28273682412232	0.0
37	1.7083796019475527E-4	0.0	0.0	6.443922439566071	0.0
38	1.7083796019475527E-4	0.0	0.0	6.61792090202443	0.0
39	1.7083796019475527E-4	0.0	0.0	6.803194669855642	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	70	0.0	45.000004	1
CACTACG	35	1.2119563E-7	45.000004	1
TAATGCG	20	7.0333405E-4	45.0	1
TAATACG	30	2.165536E-6	44.999996	1
TATCGAT	30	2.165536E-6	44.999996	13
AATTGCG	60	3.6379788E-12	41.249996	1
CTTACCG	60	3.6379788E-12	41.249996	1
AGTAACG	55	6.184564E-11	40.909092	1
ACTCACG	90	0.0	40.0	1
ACTACGG	255	0.0	39.705883	2
GTTACGG	160	0.0	39.375	2
AAACGGG	840	0.0	39.10714	3
GCTACGA	265	0.0	39.056602	9
TATCCCG	75	0.0	39.0	1
AACGGGA	1470	0.0	38.877552	4
ACGGGAG	1125	0.0	38.8	5
ACGGGAC	830	0.0	38.76506	5
TACCCGC	35	6.248387E-6	38.57143	18
TCGAACG	35	6.248387E-6	38.57143	1
TACGGGA	800	0.0	38.531254	4
>>END_MODULE