##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554123_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	787984
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26615134317448	34.0	31.0	34.0	30.0	34.0
2	32.454104398058846	34.0	31.0	34.0	30.0	34.0
3	32.54083458547382	34.0	31.0	34.0	31.0	34.0
4	36.06079691973441	37.0	35.0	37.0	35.0	37.0
5	35.90349423338545	37.0	35.0	37.0	35.0	37.0
6	35.8885967227761	37.0	35.0	37.0	35.0	37.0
7	36.01620337468781	37.0	35.0	37.0	35.0	37.0
8	36.14223892870921	37.0	36.0	37.0	35.0	37.0
9	37.78046254746289	39.0	38.0	39.0	35.0	39.0
10	37.2455252898536	39.0	37.0	39.0	34.0	39.0
11	37.19828702105627	39.0	37.0	39.0	34.0	39.0
12	37.26186572316189	39.0	37.0	39.0	34.0	39.0
13	37.25542650612195	39.0	37.0	39.0	34.0	39.0
14	38.50260792097301	40.0	38.0	41.0	34.0	41.0
15	38.49433110316961	40.0	38.0	41.0	34.0	41.0
16	38.47547539036326	40.0	38.0	41.0	34.0	41.0
17	38.40965298787793	40.0	38.0	41.0	34.0	41.0
18	38.35867606448862	40.0	38.0	41.0	34.0	41.0
19	38.341103372657315	40.0	38.0	41.0	34.0	41.0
20	38.28831296066925	40.0	38.0	41.0	34.0	41.0
21	38.22901353326971	40.0	37.0	41.0	34.0	41.0
22	38.18087423094885	40.0	37.0	41.0	34.0	41.0
23	38.09447780665597	40.0	37.0	41.0	33.0	41.0
24	38.08304102621373	40.0	37.0	41.0	33.0	41.0
25	38.0277188369307	40.0	37.0	41.0	33.0	41.0
26	37.95451430485898	40.0	37.0	41.0	33.0	41.0
27	37.88833275802554	40.0	37.0	41.0	33.0	41.0
28	37.79386129667608	40.0	37.0	41.0	33.0	41.0
29	37.725201273122295	40.0	37.0	41.0	33.0	41.0
30	37.66348301488355	40.0	36.0	41.0	33.0	41.0
31	37.57099509634713	40.0	36.0	41.0	33.0	41.0
32	37.53450958395094	40.0	36.0	41.0	33.0	41.0
33	37.43180445288229	40.0	36.0	41.0	32.0	41.0
34	37.321224796442564	40.0	36.0	41.0	32.0	41.0
35	37.28436618002396	39.0	36.0	41.0	32.0	41.0
36	37.21352210197161	39.0	35.0	41.0	32.0	41.0
37	37.15735471786229	39.0	35.0	41.0	32.0	41.0
38	37.10900855854941	39.0	35.0	41.0	31.0	41.0
39	37.02503096509574	39.0	35.0	41.0	31.0	41.0
40	36.941548305549354	39.0	35.0	41.0	31.0	41.0
41	36.89288614997259	39.0	35.0	41.0	31.0	41.0
42	36.81840367317103	39.0	35.0	41.0	31.0	41.0
43	36.74643013056102	39.0	35.0	41.0	31.0	41.0
44	36.651289873906066	39.0	35.0	41.0	31.0	41.0
45	36.495758797132936	39.0	35.0	41.0	30.0	41.0
46	36.33255370667425	39.0	35.0	40.0	30.0	41.0
47	36.2470786208857	39.0	35.0	40.0	30.0	41.0
48	36.163815508944346	38.0	35.0	40.0	30.0	41.0
49	36.08848783731649	38.0	35.0	40.0	30.0	41.0
50	35.98238289102317	38.0	35.0	40.0	29.0	41.0
51	34.9743510020508	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	9.0
13	10.0
14	12.0
15	25.0
16	62.0
17	67.0
18	160.0
19	319.0
20	590.0
21	908.0
22	1318.0
23	1969.0
24	2617.0
25	3370.0
26	4330.0
27	5571.0
28	7188.0
29	9123.0
30	11975.0
31	15497.0
32	20512.0
33	27833.0
34	41235.0
35	56327.0
36	68900.0
37	96616.0
38	160523.0
39	250776.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.016776990395744	32.421978111230686	20.43505959511868	27.12618530325489
2	23.231689983552968	31.058879368109	20.560950476151802	25.14848017218624
3	20.591915571889785	32.09291051594956	20.907911835773316	26.40726207638734
4	20.611712928181284	32.6709679384353	19.264731263578955	27.45258786980446
5	18.282858535198685	34.202090397774576	17.580052386850493	29.934998680176246
6	20.158531137688072	38.49659383946882	19.131606733131637	22.213268289711465
7	87.60647424313184	5.502269081605718	3.6401754350342137	3.2510812402282276
8	88.70218177018823	5.267112022579139	3.283442303397023	2.747263903835611
9	80.75328940689151	10.360616459217447	5.592626246218197	3.293467887672846
10	30.434755020406506	45.9514406383886	12.220045077057403	11.393759264147496
11	19.432628073666468	28.617966862271317	35.05705699608114	16.892348067981075
12	24.008101687343906	25.526279721415662	33.3510578895003	17.114560701740135
13	23.0910779914313	27.580382342788685	30.771183171231904	18.557356494548113
14	19.808524030944792	30.31064082519442	30.425871591301345	19.45496355255944
15	19.032366139414	32.606245812097704	30.28246766431806	18.078920384170235
16	21.780137667769903	31.12613961704806	29.024066478507176	18.069656236674856
17	20.25079189425166	30.54059473288798	30.71839022112124	18.490223151739123
18	20.812351519827814	30.86115961745416	29.397170500923874	18.92931836179415
19	19.637708379865582	32.02844220187212	29.34805782858535	18.98579158967695
20	21.80539198765457	31.119032873763935	29.165820625799512	17.909754512781987
21	21.11933744847611	31.908642815082537	28.288772360860122	18.68324737558123
22	20.501558407277304	30.40924688826169	29.40732299132977	19.68187171313123
23	18.96180633109302	32.75916769883652	29.00122337509391	19.27780259497655
24	18.365220613616522	31.89861723080672	30.60683973278645	19.12932242279031
25	19.585549960405288	33.338367276492924	26.9065107920973	20.169571971004487
26	20.33531137688075	32.303574691871916	27.999045665901846	19.36206826534549
27	18.2639495218177	32.801173627890925	27.75513208390018	21.179744766391195
28	18.503675201526935	33.142551117789196	29.037264716034844	19.316508964649028
29	20.04710755548336	31.371702978740686	28.588270827834066	19.992918637941887
30	20.2592946049666	31.779959999187803	28.56530081829073	19.395444577554873
31	19.822737517513044	33.164886596682166	27.05245284168206	19.959923044122725
32	20.62770310057057	31.842270909053994	28.12582488984548	19.40420110052996
33	19.18478040163252	33.37923105037666	27.777213750533004	19.658774797457816
34	17.87536295153201	32.2949450750269	29.91685110357571	19.912840869865377
35	19.2283092042478	31.65609961623586	29.328260472293856	19.78733070722248
36	18.63870332392536	35.69514101809174	27.67226238096205	17.993893277020852
37	18.463572864423643	34.52214765782046	28.069224755832607	18.945054721923288
38	18.799747202988893	33.27884830148836	29.383972263396213	18.53743223212654
39	19.52882292026234	32.49088813986071	28.58357530102134	19.396713638855612
40	20.208912871327335	32.573250218278545	27.993334890048526	19.22450202034559
41	18.327148774594406	32.40167313041889	28.970638997746146	20.300539097240552
42	19.429328514284556	31.93605453917846	27.967446039513494	20.667170907023493
43	17.959755528031028	32.32540254624459	29.316331296066927	20.398510629657455
44	19.868423724339582	32.10103250827428	27.68254177749802	20.34800198988812
45	19.451537087047452	31.922856301650793	28.718984141809983	19.906622469491765
46	17.75353306666125	33.37301265000305	28.666445003959474	20.20700927937623
47	18.04554407196085	32.974908119961825	28.85781944811062	20.1217283599667
48	18.3696623281691	31.93630835143861	29.86976892931836	19.82426039107393
49	18.67436394647607	32.734040285081925	28.616190176450285	19.975405591991716
50	18.5289295214116	31.784782432130605	29.115311986030175	20.570976060427622
51	18.601012203293468	30.397952242685133	29.785630165079496	21.215405388941907
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7225.0
1	5533.5
2	3842.0
3	2546.0
4	1250.0
5	1293.0
6	1336.0
7	1717.5
8	2099.0
9	2596.5
10	3094.0
11	3682.0
12	4270.0
13	4648.0
14	5026.0
15	5246.0
16	5466.0
17	5418.0
18	5370.0
19	5681.5
20	5993.0
21	6376.0
22	6759.0
23	7705.0
24	8651.0
25	8888.0
26	11384.5
27	13644.0
28	16778.0
29	19912.0
30	21278.0
31	22644.0
32	27392.0
33	32140.0
34	34557.0
35	36974.0
36	40641.5
37	44309.0
38	45780.5
39	47252.0
40	48754.0
41	50256.0
42	50467.5
43	50679.0
44	54248.0
45	57817.0
46	59840.5
47	61864.0
48	60984.5
49	60105.0
50	55645.0
51	51185.0
52	46695.0
53	42205.0
54	37090.5
55	31976.0
56	28300.0
57	24624.0
58	21939.5
59	19255.0
60	17927.0
61	16599.0
62	14728.5
63	12858.0
64	10583.0
65	8308.0
66	6537.0
67	4766.0
68	3892.0
69	3018.0
70	2482.5
71	1947.0
72	1734.0
73	1521.0
74	1298.0
75	969.5
76	864.0
77	612.5
78	361.0
79	267.5
80	174.0
81	123.0
82	72.0
83	49.5
84	27.0
85	19.0
86	11.0
87	15.0
88	19.0
89	12.5
90	6.0
91	7.0
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	787984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.919372460812912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.76531299907121	19.587965628496292
2	9.22530463207298	4.966788229104703
3	3.9062029459391896	3.154575960277851
4	2.3362339827427183	2.515598109482384
5	1.582016416795792	2.1293444581423278
6	1.1741486839418356	1.8964407448442144
7	0.8742547631399641	1.6474072716223125
8	0.7748280183029116	1.6686307214215712
9	0.6282491170008331	1.522086478085003
>10	5.570158588472067	32.01453718063182
>50	0.8692715381522115	16.310454599347025
>100	0.2892837199340262	10.708925529426901
>500	0.0023672972171360574	0.37853336375037666
>1k	0.001893837773708846	0.7167917972162015
>5k	4.734594434272115E-4	0.7819199281510305
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6135	0.7785691080021929	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1725	0.21891307437714472	No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	1557	0.19759284452476192	TruSeq Adapter, Index 14 (95% over 22bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1310	0.16624703039655628	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1032	0.1309671262360657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2362992141972426	0.0
2	0.0	0.0	0.0	0.35026091900343154	0.0
3	0.0	0.0	0.0	0.45584681922475584	0.0
4	0.0	0.0	0.0	0.9519228816828769	0.0
5	0.0	0.0	0.0	1.039741923693882	0.0
6	0.0	0.0	0.0	1.6652622388271843	0.0
7	0.0	0.0	0.0	2.14128713273366	0.0
8	0.0	0.0	0.0	2.6203577737619037	0.0
9	0.0	0.0	0.0	3.2005726004588926	0.0
10	0.0	0.0	0.0	4.166708968710025	0.0
11	0.0	0.0	0.0	4.918627789396739	0.0
12	0.0	0.0	0.0	5.317366850088327	0.0
13	0.0	0.0	0.0	5.499477146744097	0.0
14	0.0	0.0	0.0	5.671181140733822	0.0
15	0.0	0.0	0.0	5.8200420313102805	0.0
16	0.0	0.0	0.0	6.11205303660988	0.0
17	0.0	0.0	0.0	6.514218562813459	0.0
18	0.0	0.0	0.0	6.97679140693212	0.0
19	0.0	0.0	0.0	7.202811224593392	0.0
20	0.0	0.0	0.0	7.445836463684542	0.0
21	0.0	0.0	0.0	7.708785965197263	0.0
22	0.0	0.0	0.0	8.033158033665659	0.0
23	0.0	0.0	0.0	8.361464192166338	0.0
24	0.0	0.0	0.0	8.612865235842353	0.0
25	0.0	0.0	0.0	8.832793559260086	0.0
26	0.0	0.0	0.0	9.037746959329123	0.0
27	0.0	0.0	0.0	9.257675282746858	0.0
28	0.0	0.0	0.0	9.494862839854616	0.0
29	0.0	0.0	0.0	9.74385266705923	0.0
30	0.0	0.0	0.0	10.075839103332047	0.0
31	1.2690613007370708E-4	0.0	0.0	10.325209648926881	0.0
32	1.2690613007370708E-4	0.0	0.0	10.605799102519848	0.0
33	1.2690613007370708E-4	0.0	0.0	10.873190318585149	0.0
34	1.2690613007370708E-4	0.0	0.0	11.125098986781458	0.0
35	1.2690613007370708E-4	0.0	0.0	11.407718938455604	0.0
36	1.2690613007370708E-4	0.0	0.0	11.696684196633434	0.0
37	1.2690613007370708E-4	0.0	0.0	11.973339560194116	0.0
38	1.2690613007370708E-4	0.0	0.0	12.264335316453126	0.0
39	1.2690613007370708E-4	0.0	0.0	12.561041848565454	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	20	7.0319336E-4	45.000004	9
TACTTCG	20	7.0319336E-4	45.000004	14
CGAAACG	80	0.0	45.000004	1
TAGGTCG	20	7.0319336E-4	45.000004	1
GTCTCGC	20	7.0319336E-4	45.000004	31
TAACGCG	35	1.2112832E-7	45.000004	1
ACGGCAA	20	7.0319336E-4	45.000004	34
ACGTGCG	70	0.0	45.000004	1
CGAATCT	30	2.1646301E-6	45.000004	33
GCCTATC	20	7.0319336E-4	45.000004	37
TTAACGC	30	2.1646301E-6	45.000004	24
ACTCGCG	30	2.1646301E-6	45.000004	1
ACTCGAT	20	7.0319336E-4	45.000004	38
TACTCGA	20	7.0319336E-4	45.000004	37
ACGTCAC	20	7.0319336E-4	45.000004	42
TTACGTA	20	7.0319336E-4	45.000004	34
ACCCGTA	20	7.0319336E-4	45.000004	31
CGTCCAA	20	7.0319336E-4	45.000004	13
CACCCGT	20	7.0319336E-4	45.000004	30
CCGCACG	60	0.0	45.000004	1
>>END_MODULE