FastQCFastQC Report
Sat 18 Jun 2016
SRR3554120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869414
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22030.2533890643582919No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14420.1658588428527721No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA11960.13756392236609946No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10870.12502674215045997No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9870.11352474195262556No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9830.11306466194471218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG302.1648884E-645.0000041
CTCGTAC207.032337E-445.042
ATAGCCG253.8900187E-545.01
CCGTAGG253.8900187E-545.02
AATTGCG351.2114651E-745.01
AATTCGG207.032337E-445.02
CGTACAG351.2114651E-745.01
CGTAAAC207.032337E-445.045
CGACTAG253.8900187E-545.01
CGATACG207.032337E-445.01
TTTCACG750.042.01
CATACCG750.042.01
TATAGCG750.042.01
AATCCGG1200.041.2500042
TAACACG603.6379788E-1241.2500041
CTAACCG556.002665E-1140.9090921
CCAAACG556.002665E-1140.9090921
ATACCGG1500.040.52
ACGGGAG8900.040.196635
CAAACGG2750.040.0909082