##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554120_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	869414
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29198517622215	34.0	31.0	34.0	30.0	34.0
2	32.48183834168762	34.0	31.0	34.0	31.0	34.0
3	32.56252027227535	34.0	31.0	34.0	31.0	34.0
4	36.099186348506	37.0	35.0	37.0	35.0	37.0
5	35.921891066856524	37.0	35.0	37.0	35.0	37.0
6	35.894214953980494	37.0	35.0	37.0	35.0	37.0
7	35.95472352642125	37.0	35.0	37.0	35.0	37.0
8	36.053827060525826	37.0	35.0	37.0	35.0	37.0
9	37.66831106929495	39.0	38.0	39.0	35.0	39.0
10	37.275575272539896	39.0	37.0	39.0	34.0	39.0
11	37.20763640797134	39.0	37.0	39.0	34.0	39.0
12	37.15415440745146	39.0	37.0	39.0	34.0	39.0
13	37.139918381806595	39.0	37.0	39.0	33.0	39.0
14	38.37373334222821	40.0	38.0	41.0	34.0	41.0
15	38.393417865366786	40.0	38.0	41.0	34.0	41.0
16	38.37960971412929	40.0	38.0	41.0	34.0	41.0
17	38.34235358528848	40.0	38.0	41.0	34.0	41.0
18	38.28353235627676	40.0	38.0	41.0	34.0	41.0
19	38.293026107240046	40.0	38.0	41.0	34.0	41.0
20	38.19843365761306	40.0	37.0	41.0	34.0	41.0
21	38.15196787721385	40.0	37.0	41.0	34.0	41.0
22	38.08441317945191	40.0	37.0	41.0	33.0	41.0
23	38.01249117221485	40.0	37.0	41.0	33.0	41.0
24	37.94501698845429	40.0	37.0	41.0	33.0	41.0
25	37.86652963950431	40.0	37.0	41.0	33.0	41.0
26	37.77616992595012	40.0	37.0	41.0	33.0	41.0
27	37.70133561226297	40.0	37.0	41.0	33.0	41.0
28	37.61220086172985	40.0	36.0	41.0	33.0	41.0
29	37.50183686943159	40.0	36.0	41.0	32.0	41.0
30	37.46809115105117	40.0	36.0	41.0	32.0	41.0
31	37.41222708629031	40.0	36.0	41.0	32.0	41.0
32	37.34544877354172	39.0	36.0	41.0	32.0	41.0
33	37.24382975199387	39.0	36.0	41.0	32.0	41.0
34	37.14686213932603	39.0	36.0	41.0	31.0	41.0
35	37.087120750298475	39.0	35.0	41.0	31.0	41.0
36	36.94933483932856	39.0	35.0	41.0	31.0	41.0
37	36.85621004492681	39.0	35.0	41.0	31.0	41.0
38	36.80069794137201	39.0	35.0	41.0	31.0	41.0
39	36.735225105645874	39.0	35.0	41.0	31.0	41.0
40	36.639001672390826	39.0	35.0	41.0	30.0	41.0
41	36.5583369948034	39.0	35.0	41.0	30.0	41.0
42	36.45999834371197	39.0	35.0	40.0	30.0	41.0
43	36.350501602228626	39.0	35.0	40.0	30.0	41.0
44	36.24203774036305	39.0	35.0	40.0	30.0	41.0
45	36.11129220371423	38.0	35.0	40.0	29.0	41.0
46	36.02083817375841	38.0	35.0	40.0	29.0	41.0
47	35.851629948448036	38.0	35.0	40.0	29.0	41.0
48	35.74072536214047	38.0	34.0	40.0	28.0	41.0
49	35.59564603284511	38.0	34.0	40.0	28.0	41.0
50	35.48058232326601	38.0	34.0	40.0	28.0	41.0
51	34.4034131035387	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	9.0
12	11.0
13	19.0
14	32.0
15	36.0
16	64.0
17	153.0
18	260.0
19	447.0
20	711.0
21	1182.0
22	1717.0
23	2492.0
24	3353.0
25	4293.0
26	5274.0
27	6839.0
28	8868.0
29	11198.0
30	14355.0
31	18292.0
32	24181.0
33	32783.0
34	50309.0
35	62503.0
36	76657.0
37	110658.0
38	181364.0
39	251256.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.84954463581217	31.47821406142528	20.030733344528613	26.641507958233934
2	24.75679020581679	29.29030358379322	19.42756845415418	26.52533775623581
3	22.230375862362465	29.593841369014072	20.357275130145133	27.81850763847833
4	22.60580114881978	31.410467280260036	18.799789283356375	27.183942287563806
5	20.52623951305132	33.202593931084614	16.265438559765542	30.005727996098518
6	21.007138141322777	38.12257451570828	18.852698484266416	22.017588858702528
7	85.76466447515223	5.9075423216097285	4.411247115873451	3.9165460873645928
8	86.69862689121638	5.357056592141373	4.189833612065138	3.7544829045771064
9	77.69060539627841	10.253228036355521	6.892113538544353	5.164053028821712
10	34.70728559696531	35.849779276616204	14.250978245116826	15.191956881301657
11	26.311975652565984	29.920843234638504	25.771611683271722	17.995569429523794
12	27.173935547391693	25.799791583756416	28.338628087424407	18.68764478142749
13	24.942087429003905	26.65162971840803	28.019562601936478	20.38672025065159
14	22.31560568382842	28.280197926419405	27.486214852762895	21.91798153698928
15	21.43742796872376	29.590160728950764	28.582010411610582	20.390400890714897
16	23.00998143577168	28.259379306061323	27.793548298049032	20.937090960117967
17	22.555422387953264	28.096741023263945	27.84220175888587	21.50563482989692
18	22.74727575125314	27.882803819584222	28.15080042419377	21.219120004968865
19	23.579100405560528	28.629398652425657	27.127237426588486	20.664263515425333
20	24.58736574290269	27.505883273101194	27.555916973961768	20.350834010034344
21	24.42357726008553	28.605819552020094	26.6204592978719	20.350143890022476
22	23.115224737581865	28.182660964741768	26.030521707724972	22.67159258995139
23	22.30778432369389	29.19656228218087	27.294131449459062	21.201521944666176
24	21.314586606610888	28.192092604903994	28.687023673416807	21.80629711506831
25	22.52586224744483	30.399096402864455	25.632322460875944	21.442718888814767
26	23.501001824217234	29.300770403973253	25.49602375853161	21.70220401327791
27	22.461221006333002	27.94502964065451	26.338775313026936	23.254974039985555
28	22.15618796108643	29.80582323266016	27.02394946481193	21.014039341441475
29	22.15572788107852	28.949959397939303	26.413423294310878	22.4808894266713
30	23.234960559641323	27.815517118426897	27.34899599040273	21.600526331529053
31	23.166523658464207	29.296284623896096	25.020761110357093	22.516430607282604
32	23.031720216145587	28.822862295753232	26.571460777029127	21.573956711072057
33	22.27097792306082	29.41556036594764	26.283220652071392	22.030241058920147
34	22.219333942172543	29.305486224054363	27.533373053574017	20.941806780199077
35	23.67284170717288	28.269156006229483	27.669211675910443	20.3887906106872
36	23.091530617174328	31.219764116979942	25.366741276307952	20.32196398953778
37	21.661601952579552	31.241732937357806	26.588138677315985	20.508526432746656
38	21.744186314000004	30.018725256322078	27.40202021131475	20.835068218363173
39	22.065897259533433	28.71129289383424	26.86361158205412	22.359198264578207
40	22.502513187043228	29.066474659943363	27.88441409961192	20.546598053401485
41	21.050500682068613	28.407064988601515	29.54679818820493	20.99563614112494
42	21.93811003733549	29.136176781142243	26.923537003084835	22.00217617843743
43	22.043583379149634	28.45686864945814	27.393048651160434	22.106499320231787
44	22.188738621646305	28.460089209513534	27.510599093182304	21.840573075657858
45	21.960768977725227	28.92246961746648	27.26560648896843	21.851154915839864
46	20.84726033857288	29.736006091459306	27.77836565778789	21.63836791217993
47	21.718651873560813	29.507001267520423	28.174724584605265	20.599622274313504
48	21.250405445506974	28.937882297731576	28.030259462120462	21.781452794640987
49	20.564196113704174	29.686547490608618	28.348749847598498	21.400506548088714
50	20.791360617611403	28.111693623521127	29.22048644259237	21.876459316275103
51	21.85932133598033	27.504503033077455	28.30366200682299	22.332513624119237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2884.0
1	2373.5
2	1863.0
3	1322.0
4	781.0
5	820.0
6	859.0
7	1008.5
8	1158.0
9	1347.0
10	1536.0
11	1797.0
12	2058.0
13	2223.0
14	2388.0
15	2387.5
16	2387.0
17	2474.5
18	2562.0
19	2768.5
20	2975.0
21	3305.5
22	3636.0
23	4850.5
24	6065.0
25	7098.5
26	8945.0
27	9758.0
28	13121.0
29	16484.0
30	20369.5
31	24255.0
32	25934.5
33	27614.0
34	30948.5
35	34283.0
36	38164.5
37	42046.0
38	42798.5
39	43551.0
40	44531.0
41	45511.0
42	47321.5
43	49132.0
44	51574.0
45	54016.0
46	56789.5
47	59563.0
48	59109.5
49	58656.0
50	59282.5
51	59909.0
52	57447.0
53	54985.0
54	50855.5
55	46726.0
56	43215.5
57	39705.0
58	37064.0
59	34423.0
60	32837.0
61	31251.0
62	28713.0
63	26175.0
64	23162.5
65	20150.0
66	18207.5
67	16265.0
68	13654.5
69	11044.0
70	9488.0
71	7932.0
72	7019.5
73	6107.0
74	5163.5
75	3496.0
76	2772.0
77	2264.5
78	1757.0
79	1294.5
80	832.0
81	645.5
82	459.0
83	356.0
84	253.0
85	208.5
86	164.0
87	114.0
88	64.0
89	49.0
90	34.0
91	26.0
92	18.0
93	12.5
94	7.0
95	6.5
96	6.0
97	3.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	869414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.37593156575735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59774768030798	17.452617977175493
2	8.02811983838442	3.913857995643151
3	3.3638106784756228	2.4598805669605674
4	2.0399500042666006	1.9890272680627628
5	1.4638266835173912	1.7841069530774734
6	1.1825690978571894	1.7295734040687751
7	0.9987451342815071	1.7041740131415406
8	0.8642807906783819	1.685411952573991
9	0.7965323343467732	1.7474595904754906
>10	8.619874102960534	44.40239326993731
>50	0.8998634484171897	14.581057856733203
>100	0.13851350917990785	5.119286718149127
>500	0.004269251995271132	0.7456978304010056
>1k	0.0018974453312316142	0.685454603600118
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2203	0.2533890643582919	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1442	0.1658588428527721	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1196	0.13756392236609946	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1087	0.12502674215045997	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	987	0.11352474195262556	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	983	0.11306466194471218	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6008000791337614E-4	0.0	0.0	0.08350452143627776	0.0
2	4.6008000791337614E-4	0.0	0.0	0.12583188216430838	0.0
3	4.6008000791337614E-4	0.0	0.0	0.1693094429121224	0.0
4	4.6008000791337614E-4	0.0	0.0	0.32792202564025885	0.0
5	4.6008000791337614E-4	0.0	0.0	0.368754126342571	0.0
6	4.6008000791337614E-4	0.0	0.0	0.706107792145054	0.0
7	5.751000098917202E-4	0.0	0.0	0.9500652163411217	0.0
8	5.751000098917202E-4	0.0	0.0	1.2740765619141168	0.0
9	5.751000098917202E-4	0.0	0.0	1.5699080070024176	0.0
10	5.751000098917202E-4	0.0	0.0	2.049196355246177	0.0
11	5.751000098917202E-4	0.0	0.0	2.371367380787519	0.0
12	5.751000098917202E-4	0.0	0.0	2.533890643582919	0.0
13	5.751000098917202E-4	0.0	0.0	2.6131394249459983	0.0
14	5.751000098917202E-4	0.0	0.0	2.6931933463229254	0.0
15	5.751000098917202E-4	0.0	0.0	2.7609401274881704	0.0
16	5.751000098917202E-4	0.0	0.0	2.873199649419034	0.0
17	5.751000098917202E-4	0.0	0.0	3.006622851713913	0.0
18	5.751000098917202E-4	0.0	0.0	3.1783477146675807	0.0
19	5.751000098917202E-4	0.0	0.0	3.2569063760187897	0.0
20	5.751000098917202E-4	0.0	0.0	3.351682857648945	0.0
21	5.751000098917202E-4	0.0	0.0	3.4531304993938448	0.0
22	5.751000098917202E-4	0.0	0.0	3.5687256013820803	0.0
23	5.751000098917202E-4	0.0	0.0	3.6986982036176093	0.0
24	5.751000098917202E-4	0.0	0.0	3.7938197452536997	0.0
25	5.751000098917202E-4	0.0	0.0	3.88549068683044	0.0
26	5.751000098917202E-4	0.0	0.0	3.9787719084348767	0.0
27	5.751000098917202E-4	0.0	0.0	4.0755037300986645	0.0
28	5.751000098917202E-4	0.0	0.0	4.166944631671448	0.0
29	5.751000098917202E-4	0.0	0.0	4.270117573446022	0.0
30	5.751000098917202E-4	0.0	0.0	4.407911535816078	0.0
31	5.751000098917202E-4	0.0	0.0	4.51062439758274	0.0
32	5.751000098917202E-4	0.0	0.0	4.628519899610542	0.0
33	5.751000098917202E-4	0.0	0.0	4.7509011817155	0.0
34	5.751000098917202E-4	0.0	0.0	4.87316744381848	0.0
35	5.751000098917202E-4	0.0	0.0	5.009236106158861	0.0
36	5.751000098917202E-4	0.0	0.0	5.139668788402303	0.0
37	5.751000098917202E-4	0.0	0.0	5.261935050505283	0.0
38	5.751000098917202E-4	0.0	0.0	5.39547327280214	0.0
39	5.751000098917202E-4	0.0	0.0	5.533727315180109	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGCG	30	2.1648884E-6	45.000004	1
CTCGTAC	20	7.032337E-4	45.0	42
ATAGCCG	25	3.8900187E-5	45.0	1
CCGTAGG	25	3.8900187E-5	45.0	2
AATTGCG	35	1.2114651E-7	45.0	1
AATTCGG	20	7.032337E-4	45.0	2
CGTACAG	35	1.2114651E-7	45.0	1
CGTAAAC	20	7.032337E-4	45.0	45
CGACTAG	25	3.8900187E-5	45.0	1
CGATACG	20	7.032337E-4	45.0	1
TTTCACG	75	0.0	42.0	1
CATACCG	75	0.0	42.0	1
TATAGCG	75	0.0	42.0	1
AATCCGG	120	0.0	41.250004	2
TAACACG	60	3.6379788E-12	41.250004	1
CTAACCG	55	6.002665E-11	40.909092	1
CCAAACG	55	6.002665E-11	40.909092	1
ATACCGG	150	0.0	40.5	2
ACGGGAG	890	0.0	40.19663	5
CAAACGG	275	0.0	40.090908	2
>>END_MODULE