FastQCFastQC Report
Sat 18 Jun 2016
SRR3554119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857232
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21860.2550068126248204No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14940.17428187468503276No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA11090.1293698788659313No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11080.1292532243313362No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9780.11408813483397727No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9740.113621516695597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAT1250.045.00000411
CGAATAT1250.045.00000413
CCACGCG502.1827873E-1145.0000041
ACGAATA1250.045.00000412
GATAACG351.211447E-745.01
TAGCGCG351.211447E-745.01
CCGTCGG207.032283E-445.02
CCGTAGA207.032283E-445.041
TATACCG406.8121153E-945.01
CAATCGA207.032283E-445.040
ATACCGG850.045.02
GCTACGA1300.043.269239
ATAACGG1200.041.2499962
AGATACG501.0804797E-940.5000041
AATTGCG451.9274012E-840.01
TAGCGAG403.4568438E-739.3751
TTAACGG800.039.3752
TCGACAG403.4568438E-739.3751
CTTAACG356.2464314E-638.5714261
TAACGCG356.2464314E-638.5714261