##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554119_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857232
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34925434421487	34.0	31.0	34.0	30.0	34.0
2	32.52639308845213	34.0	31.0	34.0	31.0	34.0
3	32.60340141291972	34.0	31.0	34.0	31.0	34.0
4	36.1193819176139	37.0	35.0	37.0	35.0	37.0
5	35.961444509770985	37.0	35.0	37.0	35.0	37.0
6	35.93547254418874	37.0	35.0	37.0	35.0	37.0
7	35.97712054612987	37.0	35.0	37.0	35.0	37.0
8	36.07773391567277	37.0	35.0	37.0	35.0	37.0
9	37.69171472833492	39.0	38.0	39.0	35.0	39.0
10	37.315538850626204	39.0	37.0	39.0	34.0	39.0
11	37.265837019616626	39.0	37.0	39.0	34.0	39.0
12	37.2258443455214	39.0	37.0	39.0	34.0	39.0
13	37.2090204285421	39.0	37.0	39.0	34.0	39.0
14	38.44932293708121	40.0	38.0	41.0	34.0	41.0
15	38.46460934729455	40.0	38.0	41.0	34.0	41.0
16	38.46291319036153	40.0	38.0	41.0	34.0	41.0
17	38.432416195382345	40.0	38.0	41.0	34.0	41.0
18	38.373713300483416	40.0	38.0	41.0	34.0	41.0
19	38.37395827500607	40.0	38.0	41.0	34.0	41.0
20	38.290336804972284	40.0	38.0	41.0	34.0	41.0
21	38.237795602590666	40.0	37.0	41.0	34.0	41.0
22	38.17939484293633	40.0	37.0	41.0	34.0	41.0
23	38.09132533549844	40.0	37.0	41.0	33.0	41.0
24	38.066620238161896	40.0	37.0	41.0	33.0	41.0
25	37.986758543778116	40.0	37.0	41.0	33.0	41.0
26	37.92198494689885	40.0	37.0	41.0	33.0	41.0
27	37.852687487168	40.0	37.0	41.0	33.0	41.0
28	37.73358087425574	40.0	37.0	41.0	33.0	41.0
29	37.65615026037292	40.0	36.0	41.0	33.0	41.0
30	37.61672802695186	40.0	36.0	41.0	33.0	41.0
31	37.52986706049238	40.0	36.0	41.0	33.0	41.0
32	37.46463734438285	40.0	36.0	41.0	32.0	41.0
33	37.38293484144316	40.0	36.0	41.0	32.0	41.0
34	37.293016359631935	40.0	36.0	41.0	32.0	41.0
35	37.198941476753085	39.0	36.0	41.0	32.0	41.0
36	37.10609613266887	39.0	35.0	41.0	31.0	41.0
37	37.00212077943894	39.0	35.0	41.0	31.0	41.0
38	36.939235819474774	39.0	35.0	41.0	31.0	41.0
39	36.88118502342423	39.0	35.0	41.0	31.0	41.0
40	36.765105595684716	39.0	35.0	41.0	31.0	41.0
41	36.6887085409784	39.0	35.0	41.0	31.0	41.0
42	36.61879397875954	39.0	35.0	41.0	31.0	41.0
43	36.50919821005282	39.0	35.0	41.0	30.0	41.0
44	36.40735413516994	39.0	35.0	40.0	30.0	41.0
45	36.27019173339306	39.0	35.0	40.0	30.0	41.0
46	36.13700608470052	38.0	35.0	40.0	30.0	41.0
47	36.01193959161581	38.0	35.0	40.0	29.0	41.0
48	35.9073226384456	38.0	35.0	40.0	29.0	41.0
49	35.778212899191814	38.0	34.0	40.0	28.0	41.0
50	35.68563586053717	38.0	34.0	40.0	28.0	41.0
51	34.64352124045766	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	2.0
10	5.0
11	5.0
12	10.0
13	13.0
14	31.0
15	44.0
16	55.0
17	139.0
18	231.0
19	425.0
20	691.0
21	1066.0
22	1676.0
23	2275.0
24	3069.0
25	4081.0
26	4982.0
27	6379.0
28	8082.0
29	10435.0
30	13566.0
31	17339.0
32	22253.0
33	30649.0
34	47877.0
35	59969.0
36	73619.0
37	106720.0
38	178400.0
39	263041.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.747671575489484	31.5740662971051	19.993653993318027	26.68460813408739
2	24.741493551337328	29.12280456165892	19.513503928924724	26.622197958079024
3	22.158528846333315	29.694411781174757	20.332302107247514	27.814757265244417
4	22.51747484928234	31.318242902738113	18.81905948448028	27.34522276349926
5	20.556278813670044	33.098391100658866	16.341200515146426	30.00412957052467
6	20.954654049312204	38.09902103514568	18.850906172424732	22.09541874311738
7	85.71203594826137	5.815811822237154	4.467635365921944	4.0045168635795205
8	86.58461186703249	5.330528958321668	4.218227970957687	3.86663120368815
9	77.5715325606137	10.309111185770012	6.9501605166396025	5.169195736976688
10	34.842843010993526	35.65184220841032	14.336025719991788	15.169289060604363
11	26.283316535080353	29.794034753718947	25.760937529163634	18.16171118203707
12	27.264614480094068	25.701093752916364	28.305406237751274	18.728885529238294
13	24.911809171846127	26.6370130839726	28.110126546839133	20.341051197342143
14	22.322078503835602	28.16612072344476	27.53980252720384	21.9719982455158
15	21.44798607611475	29.481050637400376	28.663535658958132	20.407427627526737
16	23.086282359967896	28.22538122701906	27.775444687085876	20.91289172592717
17	22.60263265953674	28.07688000447953	27.775444687085876	21.545042648897848
18	22.737251992459452	27.843337626220205	28.195401011628125	21.22400936969222
19	23.48454094107546	28.693165910745282	27.10258133154152	20.719711816637737
20	24.597775164716204	27.501306530787467	27.613644847602515	20.287273456893818
21	24.373215185620694	28.63238889822125	26.65264479160834	20.341751124549713
22	23.143793045523264	28.103593892901806	26.055606883550773	22.69700617802415
23	22.413302349888944	29.222310879668516	27.294594695485003	21.06979207495754
24	21.318732851783416	28.156088433469584	28.665752095115444	21.85942661963156
25	22.485511506803295	30.400638333613305	25.764437165201485	21.349412994381918
26	23.544034753718947	29.17273270246561	25.56589114732068	21.717341396494767
27	22.52389084868507	27.8908160218004	26.43310095750042	23.15219217201411
28	22.241236911361216	29.75612202997555	27.00412490434328	20.99851615431995
29	22.1872258618437	28.976169811672918	26.48162924389197	22.35497508259141
30	23.27876234204976	27.782560613696177	27.414515557048734	21.524161487205333
31	23.21436903895328	29.351097485861473	24.902710118147713	22.531823357037535
32	23.05140265412397	28.772607648804527	26.577519271329113	21.598470425742388
33	22.258851746085075	29.409774716762787	26.297198424697164	22.03417511245497
34	22.24030367508446	29.265356402934096	27.43119715549583	21.06314276648562
35	23.667688560389717	28.225731190622845	27.708484984228303	20.39809526475913
36	23.03577094648823	31.15177688187095	25.49158220878362	20.320869962857195
37	21.535826940664837	31.19517236874032	26.609715922877353	20.65928476771749
38	21.796316516415626	30.00284637064412	27.418715120294156	20.7821219926461
39	22.01247736902029	28.703548164324243	26.870205498628142	22.413768968027327
40	22.49834350560875	28.958088358810684	27.91811318289564	20.625454952684922
41	21.008081826156747	28.443058588573457	29.545443940496856	21.003415644772943
42	21.901422252085784	29.14123597812494	27.00214177725517	21.955199992534112
43	22.105567687627154	28.396980047408405	27.29436138641581	22.203090878548633
44	22.273316908374863	28.37994848535752	27.50212311252963	21.844611493737982
45	22.086552998488155	28.76082554081042	27.403550030796797	21.749071429904625
46	20.93015659704724	29.73804057711331	27.690053567762284	21.64174925807716
47	21.791417025962634	29.398109263303283	28.239496425705056	20.570977285029024
48	21.280703473505422	28.8796965115628	28.004904156634375	21.834695858297405
49	20.50180114601415	29.749589376038227	28.323371036078914	21.425238441868714
50	20.755758067827614	28.1083767288202	29.160600630867723	21.975264572484463
51	21.810898333240008	27.54330216324169	28.30692274670101	22.338876756817292
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2876.0
1	2334.5
2	1793.0
3	1304.0
4	815.0
5	860.0
6	905.0
7	1065.0
8	1225.0
9	1316.0
10	1407.0
11	1730.0
12	2053.0
13	2225.5
14	2398.0
15	2358.5
16	2319.0
17	2426.5
18	2534.0
19	2735.5
20	2937.0
21	3303.0
22	3669.0
23	4935.0
24	6201.0
25	7045.5
26	8745.0
27	9600.0
28	12853.5
29	16107.0
30	20059.0
31	24011.0
32	25648.5
33	27286.0
34	30314.0
35	33342.0
36	37148.5
37	40955.0
38	41801.0
39	42647.0
40	43655.5
41	44664.0
42	46510.5
43	48357.0
44	50622.0
45	52887.0
46	55883.5
47	58880.0
48	58484.0
49	58088.0
50	58543.0
51	58998.0
52	56785.5
53	54573.0
54	50564.5
55	46556.0
56	43182.0
57	39808.0
58	36819.5
59	33831.0
60	32192.5
61	30554.0
62	28140.0
63	25726.0
64	22876.0
65	20026.0
66	18153.5
67	16281.0
68	13666.0
69	11051.0
70	9393.0
71	7735.0
72	6832.0
73	5929.0
74	5055.5
75	3434.0
76	2686.0
77	2174.5
78	1663.0
79	1245.5
80	828.0
81	632.5
82	437.0
83	342.0
84	247.0
85	193.5
86	140.0
87	107.5
88	75.0
89	52.5
90	30.0
91	19.0
92	8.0
93	10.0
94	12.0
95	10.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	857232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.038249767842824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01245693572523	17.070151764491452
2	8.040690698077595	3.8656826261271946
3	3.4152289242711493	2.4628837768797314
4	2.0853580764094595	2.005134331844754
5	1.5704734534335913	1.8875716563701672
6	1.2342932984032062	1.7802150356274495
7	1.0303936080644531	1.7338201236889446
8	0.8696026256628936	1.6722980091565247
9	0.8024661952360888	1.7360894548202062
>10	8.901059049885097	45.04876356880201
>50	0.8929728032061053	14.2470383000248
>100	0.13865734067860783	5.056422871808498
>500	0.004394070655307994	0.7418449766201262
>1k	0.0019529202912479976	0.692083503738156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2186	0.2550068126248204	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1494	0.17428187468503276	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1109	0.1293698788659313	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1108	0.1292532243313362	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	978	0.11408813483397727	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	974	0.113621516695597	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3330906919013757E-4	0.0	0.0	0.08305802863168897	0.0
2	2.3330906919013757E-4	0.0	0.0	0.12762006084700525	0.0
3	2.3330906919013757E-4	0.0	0.0	0.1707822386471807	0.0
4	2.3330906919013757E-4	0.0	0.0	0.3407478955521959	0.0
5	2.3330906919013757E-4	0.0	0.0	0.3877596729940086	0.0
6	2.3330906919013757E-4	0.0	0.0	0.7225581872818561	0.0
7	2.3330906919013757E-4	0.0	0.0	0.9789648543218172	0.0
8	2.3330906919013757E-4	0.0	0.0	1.3232123859118652	0.0
9	2.3330906919013757E-4	0.0	0.0	1.6322302480542024	0.0
10	2.3330906919013757E-4	0.0	0.0	2.125678929391343	0.0
11	2.3330906919013757E-4	0.0	0.0	2.45931089833324	0.0
12	2.3330906919013757E-4	0.0	0.0	2.625543610131213	0.0
13	2.3330906919013757E-4	0.0	0.0	2.7062685480710007	0.0
14	2.3330906919013757E-4	0.0	0.0	2.7860602497340277	0.0
15	2.3330906919013757E-4	0.0	0.0	2.854769770610523	0.0
16	2.3330906919013757E-4	0.0	0.0	2.981806558784553	0.0
17	2.3330906919013757E-4	0.0	0.0	3.118059055191593	0.0
18	2.3330906919013757E-4	0.0	0.0	3.2984069656755697	0.0
19	2.3330906919013757E-4	0.0	0.0	3.378315321873192	0.0
20	2.3330906919013757E-4	0.0	0.0	3.4785215670903558	0.0
21	2.3330906919013757E-4	0.0	0.0	3.5846771935718684	0.0
22	2.3330906919013757E-4	0.0	0.0	3.7071644548966907	0.0
23	2.3330906919013757E-4	0.0	0.0	3.839567351662094	0.0
24	2.3330906919013757E-4	0.0	0.0	3.942923269313325	0.0
25	2.3330906919013757E-4	0.0	0.0	4.038113369542901	0.0
26	2.3330906919013757E-4	0.0	0.0	4.130853724545981	0.0
27	2.3330906919013757E-4	0.0	0.0	4.22312746141068	0.0
28	2.3330906919013757E-4	0.0	0.0	4.324500251973794	0.0
29	2.3330906919013757E-4	0.0	0.0	4.433222278216399	0.0
30	2.3330906919013757E-4	0.0	0.0	4.5768240103029285	0.0
31	2.3330906919013757E-4	0.0	0.0	4.686595927356888	0.0
32	2.3330906919013757E-4	0.0	0.0	4.807916643335759	0.0
33	2.3330906919013757E-4	0.0	0.0	4.939152994755212	0.0
34	2.3330906919013757E-4	0.0	0.0	5.06222352875301	0.0
35	2.3330906919013757E-4	0.0	0.0	5.196609552606529	0.0
36	2.3330906919013757E-4	0.0	0.0	5.326446049610841	0.0
37	2.3330906919013757E-4	0.0	0.0	5.455465964872986	0.0
38	2.3330906919013757E-4	0.0	0.0	5.6007008604438475	0.0
39	2.3330906919013757E-4	0.0	0.0	5.745352483341732	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	125	0.0	45.000004	11
CGAATAT	125	0.0	45.000004	13
CCACGCG	50	2.1827873E-11	45.000004	1
ACGAATA	125	0.0	45.000004	12
GATAACG	35	1.211447E-7	45.0	1
TAGCGCG	35	1.211447E-7	45.0	1
CCGTCGG	20	7.032283E-4	45.0	2
CCGTAGA	20	7.032283E-4	45.0	41
TATACCG	40	6.8121153E-9	45.0	1
CAATCGA	20	7.032283E-4	45.0	40
ATACCGG	85	0.0	45.0	2
GCTACGA	130	0.0	43.26923	9
ATAACGG	120	0.0	41.249996	2
AGATACG	50	1.0804797E-9	40.500004	1
AATTGCG	45	1.9274012E-8	40.0	1
TAGCGAG	40	3.4568438E-7	39.375	1
TTAACGG	80	0.0	39.375	2
TCGACAG	40	3.4568438E-7	39.375	1
CTTAACG	35	6.2464314E-6	38.571426	1
TAACGCG	35	6.2464314E-6	38.571426	1
>>END_MODULE