##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554118_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	726467
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30828241337872	34.0	31.0	34.0	30.0	34.0
2	32.490117238635754	34.0	31.0	34.0	31.0	34.0
3	32.581460685757236	34.0	31.0	34.0	31.0	34.0
4	36.098077407507844	37.0	35.0	37.0	35.0	37.0
5	35.92981924849993	37.0	35.0	37.0	35.0	37.0
6	35.90066995472609	37.0	35.0	37.0	35.0	37.0
7	35.911011787183725	37.0	35.0	37.0	35.0	37.0
8	36.007241898117876	37.0	35.0	37.0	35.0	37.0
9	37.63300191199325	39.0	37.0	39.0	35.0	39.0
10	37.29117220740928	39.0	37.0	39.0	34.0	39.0
11	37.23214406160225	39.0	37.0	39.0	34.0	39.0
12	37.12266076779812	39.0	37.0	39.0	33.0	39.0
13	37.10268738979197	39.0	37.0	39.0	33.0	39.0
14	38.341999017161136	40.0	38.0	41.0	34.0	41.0
15	38.358257154144646	40.0	38.0	41.0	34.0	41.0
16	38.35696735020311	40.0	38.0	41.0	34.0	41.0
17	38.32264507541292	40.0	38.0	41.0	34.0	41.0
18	38.25681964906871	40.0	37.0	41.0	34.0	41.0
19	38.25551470335198	40.0	37.0	41.0	34.0	41.0
20	38.164125830904915	40.0	37.0	41.0	34.0	41.0
21	38.107293242501036	40.0	37.0	41.0	33.0	41.0
22	38.03535879812848	40.0	37.0	41.0	33.0	41.0
23	37.96714510087864	40.0	37.0	41.0	33.0	41.0
24	37.889694920760334	40.0	37.0	41.0	33.0	41.0
25	37.79651381274029	40.0	37.0	41.0	33.0	41.0
26	37.70431279053281	40.0	36.0	41.0	33.0	41.0
27	37.62381223097539	40.0	36.0	41.0	33.0	41.0
28	37.53609042117536	40.0	36.0	41.0	33.0	41.0
29	37.41477451831948	40.0	36.0	41.0	32.0	41.0
30	37.39129513109336	40.0	36.0	41.0	32.0	41.0
31	37.32582347167869	39.0	36.0	41.0	32.0	41.0
32	37.27149478228192	39.0	36.0	41.0	32.0	41.0
33	37.17762541175305	39.0	35.0	41.0	32.0	41.0
34	37.032501132191825	39.0	35.0	41.0	31.0	41.0
35	36.985139035909405	39.0	35.0	41.0	31.0	41.0
36	36.8795554374803	39.0	35.0	41.0	31.0	41.0
37	36.76277380803258	39.0	35.0	41.0	31.0	41.0
38	36.72193231075878	39.0	35.0	41.0	31.0	41.0
39	36.61963172449678	39.0	35.0	41.0	30.0	41.0
40	36.51591331746659	39.0	35.0	41.0	30.0	41.0
41	36.43916241205726	39.0	35.0	40.0	30.0	41.0
42	36.332019210783145	39.0	35.0	40.0	30.0	41.0
43	36.2316182290455	38.0	35.0	40.0	30.0	41.0
44	36.08468519561109	38.0	35.0	40.0	30.0	41.0
45	35.92681980048646	38.0	35.0	40.0	29.0	41.0
46	35.81143259088162	38.0	35.0	40.0	29.0	41.0
47	35.64724619287593	38.0	34.0	40.0	28.0	41.0
48	35.53950420321914	38.0	34.0	40.0	28.0	41.0
49	35.380057180849235	38.0	34.0	40.0	27.0	41.0
50	35.26268915174399	37.0	34.0	40.0	27.0	41.0
51	34.18219960438671	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	8.0
11	7.0
12	14.0
13	15.0
14	36.0
15	52.0
16	83.0
17	139.0
18	289.0
19	454.0
20	707.0
21	1047.0
22	1499.0
23	2123.0
24	3023.0
25	3913.0
26	4778.0
27	5978.0
28	7565.0
29	9574.0
30	12249.0
31	15898.0
32	20746.0
33	28057.0
34	43586.0
35	53935.0
36	64509.0
37	92215.0
38	147374.0
39	206502.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.3956490797242	32.19912260295374	18.850271244254728	26.554957073067325
2	24.645716873581318	29.543255233892246	19.29433821494989	26.516689677576544
3	22.374450594452327	29.9125768961288	19.841231604463797	27.87174090495508
4	22.596759384803438	31.182008267409255	18.592861065953443	27.628371281833864
5	20.35082116599928	33.670214889320505	16.430064958215585	29.54889898646463
6	21.00040332183017	37.65484185792335	18.62576001387537	22.71899480637111
7	83.93072224891152	6.681790088193957	4.9358057558017085	4.451681907092821
8	84.84735025816727	6.145082983810689	5.015644206825637	3.9919225511964065
9	76.19520225970346	10.439428081385666	7.785900804854178	5.579468854056688
10	36.14864818360641	33.03907816872618	14.989118569735446	15.823155077931963
11	27.603456179014323	29.689855148272393	24.343431979704516	18.36325669300877
12	27.72018550051138	24.78942608542439	28.105474852952717	19.384913561111517
13	25.000447370630734	26.45529666178918	26.416478656291336	22.127777311288742
14	22.220692749980383	28.118964798125724	27.098133845033566	22.562208606860327
15	21.74661753390037	29.006685782010745	28.878393650365396	20.368303033723485
16	23.334163836760652	27.222571706629484	27.605658619042572	21.837605837567295
17	22.833796992843446	26.97920208350827	28.095013262818547	22.091987660829744
18	23.133604141688473	27.844898667110822	27.427811586761685	21.593685604439017
19	23.541055546914038	27.826453231874265	26.708852570041035	21.923638651170666
20	24.497878086685287	26.97039232339528	27.11410153523835	21.417628054681078
21	24.478193778932837	27.904915157880538	26.148193930350587	21.46869713283604
22	23.41014801773515	28.17127274879657	25.86545569172447	22.55312354174381
23	22.661456060633174	29.245925830079	26.22527933133921	21.867338777948618
24	22.061291152936057	27.022012011557305	28.484707495316375	22.431989340190263
25	22.591803894739883	30.13240794144813	25.27258636662092	22.003201797191064
26	23.69908061894071	27.747716000864457	25.66916322420702	22.884040155987815
27	22.31099279113848	28.017239599321098	26.008476641058714	23.663290968481707
28	22.403908229830122	29.030224359812628	27.45382790959534	21.112039500761906
29	22.31677419621263	29.266436052842042	25.654021449012827	22.762768301932503
30	23.070696948381688	28.91845052837913	26.376834735782907	21.634017787456276
31	23.824757353052515	29.26616074783851	25.07588094159817	21.8332009575108
32	23.60052142767669	27.342879993172435	26.876513317191282	22.180085261959594
33	22.707982606229876	28.947357553749857	25.89780402963934	22.446855810380924
34	21.84944395271912	28.916798698357944	27.16833662093392	22.06542072798902
35	23.9958594127469	28.037612169582378	27.536832368159875	20.429696049510852
36	24.9071189744338	31.239547013147188	24.34384493720981	19.509489075209196
37	22.28924369585955	31.11442088904245	25.83462153132902	20.76171388376898
38	22.17334028937309	28.745008376154736	27.986267786423884	21.095383548048293
39	22.715278188823444	28.308649945558432	26.801217398725612	22.17485446689251
40	22.89766775366259	28.972547961572925	27.01320225144432	21.116582033320164
41	21.987371759488042	27.21582673404298	28.434326679670242	22.36247482679874
42	22.406661279865432	28.41491767692132	25.99773974592101	23.18068129729224
43	22.39950334977363	28.098179270359147	27.23812643932897	22.26419094053825
44	22.790849412292644	28.173199883821287	26.624609238960613	22.411341464925457
45	22.49930141355354	28.378852721458784	27.315624797822892	21.806221067164785
46	21.16090613888862	29.356598441498377	27.857287392269708	21.625208027343295
47	21.739046646303272	29.102354270737695	28.038988694600032	21.119610388359003
48	21.392024689352716	28.15034956852823	28.34829386606687	22.10933187605218
49	21.081205340366456	29.55991118660586	27.42849984927051	21.93038362375717
50	21.24101989491608	28.290755120328935	28.77818262908019	21.690042355674795
51	21.471174877867817	27.571796103608285	27.87174090495508	23.08528811356882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3409.0
1	2760.0
2	2111.0
3	1489.0
4	867.0
5	941.0
6	1015.0
7	1047.5
8	1080.0
9	1255.0
10	1430.0
11	1690.0
12	1950.0
13	2125.0
14	2300.0
15	2298.5
16	2297.0
17	2359.0
18	2421.0
19	2634.5
20	2848.0
21	2843.0
22	2838.0
23	4126.5
24	5415.0
25	5828.5
26	7615.0
27	8988.0
28	10302.5
29	11617.0
30	14838.0
31	18059.0
32	20177.0
33	22295.0
34	24465.5
35	26636.0
36	29380.0
37	32124.0
38	34089.0
39	36054.0
40	36706.5
41	37359.0
42	37692.5
43	38026.0
44	38923.5
45	39821.0
46	42975.5
47	46130.0
48	46006.0
49	45882.0
50	46728.5
51	47575.0
52	46447.0
53	45319.0
54	42035.5
55	38752.0
56	37020.0
57	35288.0
58	33295.0
59	31302.0
60	29821.5
61	28341.0
62	26776.5
63	25212.0
64	22965.0
65	20718.0
66	18218.0
67	15718.0
68	13636.0
69	11554.0
70	10165.5
71	8777.0
72	7751.0
73	6725.0
74	5805.0
75	4124.5
76	3364.0
77	2527.0
78	1690.0
79	1360.0
80	1030.0
81	765.5
82	501.0
83	387.0
84	273.0
85	199.5
86	126.0
87	91.5
88	57.0
89	38.5
90	20.0
91	16.5
92	13.0
93	10.0
94	7.0
95	6.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	726467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.47814661322521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96782235954123	18.33606729510939
2	7.7965670169805295	3.9728415507693167
3	3.2801738088283496	2.5071824765457014
4	2.0390650271374615	2.0780639086123314
5	1.4901221257060289	1.8982774995174507
6	1.2521625139851025	1.9141668068938262
7	1.0092619499876019	1.7999886753053589
8	0.8954014035460333	1.8250534589786773
9	0.7944922477372043	1.82179709738274
>10	8.577144469983988	44.53859007580537
>50	0.7671998654295641	13.099287767647901
>100	0.12569019071929863	4.887123553278664
>500	0.003808793658160565	0.7886660963493126
>1k	0.0010882267594744472	0.5328937378039651
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2624	0.36120016463239213	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1220	0.16793605215377985	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	950	0.13076987667712367	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	883	0.12154715905884231	No Hit
ACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACCATTA	856	0.1178305415111767	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	850	0.11700462650058434	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	775	0.10668068886817983	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	768	0.10571712135582208	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08548220359630926	0.0
2	0.0	0.0	0.0	0.13118283418241986	0.0
3	0.0	0.0	0.0	0.1715150171996801	0.0
4	0.0	0.0	0.0	0.3507385744982222	0.0
5	0.0	0.0	0.0	0.39754042509845594	0.0
6	0.0	0.0	0.0	0.7533721421619978	0.0
7	0.0	0.0	0.0	1.0122964979827025	0.0
8	0.0	0.0	0.0	1.3065975467571136	0.0
9	0.0	0.0	0.0	1.5964937154750318	0.0
10	0.0	0.0	0.0	2.0712571940638735	0.0
11	0.0	0.0	0.0	2.440303551296893	0.0
12	0.0	0.0	0.0	2.62475790366252	0.0
13	0.0	0.0	0.0	2.7106530647641254	0.0
14	0.0	0.0	0.0	2.8037061559575314	0.0
15	0.0	0.0	0.0	2.8772125919002516	0.0
16	0.0	0.0	0.0	3.0129379586409293	0.0
17	0.0	0.0	0.0	3.158849610512246	0.0
18	0.0	0.0	0.0	3.333668287754296	0.0
19	0.0	0.0	0.0	3.4274096414565287	0.0
20	0.0	0.0	0.0	3.5334020678158815	0.0
21	0.0	0.0	0.0	3.649856084309404	0.0
22	0.0	0.0	0.0	3.7791117834671084	0.0
23	0.0	0.0	0.0	3.9184161152536867	0.0
24	0.0	0.0	0.0	4.028125159160705	0.0
25	0.0	0.0	0.0	4.130676272975923	0.0
26	0.0	0.0	0.0	4.228134244225822	0.0
27	0.0	0.0	0.0	4.3393574656522595	0.0
28	0.0	0.0	0.0	4.444661629502785	0.0
29	0.0	0.0	0.0	4.559463815975123	0.0
30	0.0	0.0	0.0	4.708816780390575	0.0
31	0.0	0.0	0.0	4.824995491880567	0.0
32	0.0	0.0	0.0	4.949158048472952	0.0
33	0.0	0.0	0.0	5.081304450167729	0.0
34	0.0	0.0	0.0	5.205329354258349	0.0
35	0.0	0.0	0.0	5.339127585974311	0.0
36	0.0	0.0	0.0	5.468383285132016	0.0
37	0.0	0.0	0.0	5.592683494226166	0.0
38	0.0	0.0	0.0	5.745477771185753	0.0
39	0.0	0.0	0.0	5.8846444504705655	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	30	2.1643937E-6	45.000004	1
TCGCATA	25	3.8893828E-5	45.000004	34
CGATTCA	30	2.1643937E-6	45.000004	36
AAACGAT	30	2.1643937E-6	45.000004	44
AACGATA	25	3.8893828E-5	45.000004	45
ACCGGTT	20	7.031571E-4	45.0	35
ATACGCG	20	7.031571E-4	45.0	1
CCCGATT	20	7.031571E-4	45.0	42
CGATAGG	115	0.0	43.04348	2
TTACGGG	275	0.0	41.727272	3
CGGGATA	245	0.0	41.32653	6
CCTAGCG	60	3.6379788E-12	41.250004	1
TATGGGC	120	0.0	41.250004	4
CGAAACG	55	6.002665E-11	40.909092	1
GCGATAG	55	6.002665E-11	40.909092	1
AACGGGA	690	0.0	40.76087	4
CGTAACG	50	1.0804797E-9	40.500004	1
ATAGGCG	45	1.9266736E-8	40.0	1
TGCGACG	45	1.9266736E-8	40.0	1
TCAACGG	90	0.0	40.0	2
>>END_MODULE