FastQCFastQC Report
Sat 18 Jun 2016
SRR3554115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849209
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50560.5953775807840002No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16590.19535826869475006No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT11460.13494911146725955TruSeq Adapter, Index 21 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA10670.12564633676750953No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10010.11787439841075635No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9090.10704078736800952No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8740.10291930490609497No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT253.8899423E-545.044
ATTCACG207.0322456E-445.01
CATAGCG406.8121153E-945.01
ACGCTAG253.8899423E-545.01
CCGTTAA207.0322456E-445.043
AATCGAG207.0322456E-445.01
TGCGTAT207.0322456E-445.043
ATCGTAC302.1648302E-644.99999645
ATTCCCG302.1648302E-644.9999961
CGTACAC302.1648302E-644.99999636
CTCGATA302.1648302E-644.99999624
AGTTACG302.1648302E-644.9999961
GCCCGTA302.1648302E-644.99999612
TTACCGG1350.041.6666642
ACTACCG556.002665E-1140.9090921
TAGTACG451.9272193E-840.01
GTAGGGA3500.039.8571434
TATGGGC1300.039.807694
TGCGAAG1300.039.807691
CGCATAG403.4568075E-739.3751