Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554115_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 849209 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 5056 | 0.5953775807840002 | No Hit |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 1659 | 0.19535826869475006 | No Hit |
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT | 1146 | 0.13494911146725955 | TruSeq Adapter, Index 21 (95% over 22bp) |
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA | 1067 | 0.12564633676750953 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1001 | 0.11787439841075635 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 909 | 0.10704078736800952 | No Hit |
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA | 874 | 0.10291930490609497 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACACGTT | 25 | 3.8899423E-5 | 45.0 | 44 |
ATTCACG | 20 | 7.0322456E-4 | 45.0 | 1 |
CATAGCG | 40 | 6.8121153E-9 | 45.0 | 1 |
ACGCTAG | 25 | 3.8899423E-5 | 45.0 | 1 |
CCGTTAA | 20 | 7.0322456E-4 | 45.0 | 43 |
AATCGAG | 20 | 7.0322456E-4 | 45.0 | 1 |
TGCGTAT | 20 | 7.0322456E-4 | 45.0 | 43 |
ATCGTAC | 30 | 2.1648302E-6 | 44.999996 | 45 |
ATTCCCG | 30 | 2.1648302E-6 | 44.999996 | 1 |
CGTACAC | 30 | 2.1648302E-6 | 44.999996 | 36 |
CTCGATA | 30 | 2.1648302E-6 | 44.999996 | 24 |
AGTTACG | 30 | 2.1648302E-6 | 44.999996 | 1 |
GCCCGTA | 30 | 2.1648302E-6 | 44.999996 | 12 |
TTACCGG | 135 | 0.0 | 41.666664 | 2 |
ACTACCG | 55 | 6.002665E-11 | 40.909092 | 1 |
TAGTACG | 45 | 1.9272193E-8 | 40.0 | 1 |
GTAGGGA | 350 | 0.0 | 39.857143 | 4 |
TATGGGC | 130 | 0.0 | 39.80769 | 4 |
TGCGAAG | 130 | 0.0 | 39.80769 | 1 |
CGCATAG | 40 | 3.4568075E-7 | 39.375 | 1 |