##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	849209
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35088770844398	34.0	31.0	34.0	30.0	34.0
2	32.53101768822516	34.0	31.0	34.0	31.0	34.0
3	32.60715088982806	34.0	31.0	34.0	31.0	34.0
4	36.11419214822264	37.0	35.0	37.0	35.0	37.0
5	35.96221778148842	37.0	35.0	37.0	35.0	37.0
6	35.93635253512386	37.0	35.0	37.0	35.0	37.0
7	35.94503237718865	37.0	35.0	37.0	35.0	37.0
8	36.046988432765076	37.0	35.0	37.0	35.0	37.0
9	37.69874907119449	39.0	38.0	39.0	35.0	39.0
10	37.34050863803846	39.0	37.0	39.0	34.0	39.0
11	37.28344730213646	39.0	37.0	39.0	34.0	39.0
12	37.21078203363365	39.0	37.0	39.0	34.0	39.0
13	37.18554089747047	39.0	37.0	39.0	34.0	39.0
14	38.432881658107725	40.0	38.0	41.0	34.0	41.0
15	38.42127791862781	40.0	38.0	41.0	34.0	41.0
16	38.43360821658744	40.0	38.0	41.0	34.0	41.0
17	38.403013863489434	40.0	38.0	41.0	34.0	41.0
18	38.33383301401658	40.0	38.0	41.0	34.0	41.0
19	38.306488744231395	40.0	37.0	41.0	34.0	41.0
20	38.22097033827951	40.0	37.0	41.0	34.0	41.0
21	38.15728990154367	40.0	37.0	41.0	34.0	41.0
22	38.09829853428308	40.0	37.0	41.0	33.0	41.0
23	38.017542206924325	40.0	37.0	41.0	33.0	41.0
24	37.98019922068654	40.0	37.0	41.0	33.0	41.0
25	37.92497724352898	40.0	37.0	41.0	33.0	41.0
26	37.84402544014489	40.0	37.0	41.0	33.0	41.0
27	37.77217151490387	40.0	36.0	41.0	33.0	41.0
28	37.67465017445647	40.0	36.0	41.0	33.0	41.0
29	37.60079085360612	40.0	36.0	41.0	33.0	41.0
30	37.55921098339749	40.0	36.0	41.0	33.0	41.0
31	37.45773773005232	40.0	36.0	41.0	32.0	41.0
32	37.40225668828286	40.0	36.0	41.0	32.0	41.0
33	37.32699253069621	40.0	36.0	41.0	32.0	41.0
34	37.18959879134583	39.0	35.0	41.0	32.0	41.0
35	37.143379309451504	39.0	35.0	41.0	31.0	41.0
36	37.03307666310649	39.0	35.0	41.0	31.0	41.0
37	36.94366757771055	39.0	35.0	41.0	31.0	41.0
38	36.852947860891724	39.0	35.0	41.0	31.0	41.0
39	36.80894455899549	39.0	35.0	41.0	31.0	41.0
40	36.67882229227434	39.0	35.0	41.0	31.0	41.0
41	36.552662536548716	39.0	35.0	41.0	30.0	41.0
42	36.478388712319344	39.0	35.0	41.0	30.0	41.0
43	36.388170638794456	39.0	35.0	40.0	30.0	41.0
44	36.31257440747802	39.0	35.0	40.0	30.0	41.0
45	36.15718038786682	38.0	35.0	40.0	30.0	41.0
46	35.997169130331876	38.0	35.0	40.0	29.0	41.0
47	35.86484128171039	38.0	35.0	40.0	29.0	41.0
48	35.759579797199514	38.0	34.0	40.0	29.0	41.0
49	35.61960600982797	38.0	34.0	40.0	28.0	41.0
50	35.49938236641392	38.0	34.0	40.0	28.0	41.0
51	34.424307797020525	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	4.0
11	7.0
12	10.0
13	13.0
14	21.0
15	59.0
16	79.0
17	157.0
18	259.0
19	434.0
20	704.0
21	1150.0
22	1601.0
23	2280.0
24	3225.0
25	4125.0
26	5391.0
27	6627.0
28	8223.0
29	10619.0
30	13520.0
31	17623.0
32	22936.0
33	31368.0
34	49188.0
35	62925.0
36	73337.0
37	105376.0
38	171847.0
39	256002.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.38695067998573	32.102226895852496	18.855193480050257	26.65562894411152
2	24.827103810722683	30.1034256584657	19.116495468135643	25.95297506267597
3	22.51353906988739	30.351774415956495	19.76203737831323	27.372649135842885
4	22.645897535235733	31.406756169564854	18.728958360073904	27.21838793512551
5	19.57586412767646	33.391426609939366	17.029494506063877	30.003214756320297
6	21.46597598471048	38.00089259534461	18.826578616100395	21.70655280384452
7	83.57907181859825	6.904189663557498	5.288921808412299	4.227816709431954
8	83.90443341980595	6.677979154719274	5.135249390903771	4.282338034570995
9	75.21517082367238	11.037094519723649	7.957169554255785	5.790565102348185
10	36.09747423779069	33.22397666534387	14.707922313588293	15.970626783277146
11	27.380303317557868	30.217178574414543	24.13092654458443	18.271591563443156
12	27.10404623596783	25.593464035355257	27.902436267161555	19.400053461515363
13	24.823335598186077	26.726047415889376	27.512779539547978	20.93783744637657
14	22.123764585632042	27.963198694314357	27.75830213763632	22.154734582417284
15	21.35646230786532	29.618268294377476	28.399840321993764	20.625429075763446
16	22.807930674309855	27.37889023785664	28.386180551548556	21.426998536284945
17	22.394251591775404	27.515605698950434	28.487569020111657	21.6025736891625
18	23.833237754192428	27.29752039839427	27.408329398298886	21.46091244911441
19	23.16591086528758	28.854027689296746	27.301641880856188	20.678419564559487
20	24.41154062191993	27.81423654247659	27.405974265463506	20.368248570139976
21	24.323223140593186	28.926801293909982	26.644206549859927	20.105769015636906
22	23.22137424356077	28.18693631367543	25.91588172051874	22.675807722245054
23	22.7306823173094	29.235205938702958	25.91493966738459	22.11917207660305
24	21.99694068244684	27.889247523283434	28.460602749146556	21.653209045123166
25	22.55534267771538	29.018651474489786	25.705215088394024	22.720790759400806
26	22.941937732642963	28.99286276994238	25.906343432535454	22.1588560648792
27	22.082549761012896	28.313289190293556	26.719335287308542	22.884825761385006
28	22.141192568613853	28.90701817809279	28.119461757941806	20.832327495351556
29	22.547335226075088	29.455410858810964	26.076737293175178	21.920516621938766
30	22.929337771973685	28.570351939275255	27.05223331358947	21.44807697516159
31	23.052040192696968	29.636402817209902	25.166831722226213	22.14472526786692
32	22.987509553007563	29.197523813336883	26.899149679289785	20.915816954365766
33	22.827007250276434	29.422674512399183	26.33803928126056	21.41227895606382
34	20.314080514926243	29.86532172880881	27.993226637965446	21.8273711182995
35	23.201237857818274	27.95907721185244	28.06882640198114	20.770858528348146
36	23.408136277406385	30.924542721520847	26.158107132637546	19.509213868435214
37	21.732459265033697	31.670413290485616	26.273390884929388	20.3237365595513
38	21.677113643402272	30.67725377380598	27.09285935499977	20.55277322779198
39	22.358218059394094	29.045382232171352	26.751482850511476	21.84491685792308
40	23.347727120178895	28.85120152989429	27.47156471492883	20.32950663499798
41	21.704668697576217	28.167977494350627	28.277373414554013	21.849980393519143
42	22.138719679136702	28.598731289941583	26.978282142558545	22.28426688836317
43	21.7480031417472	28.21602220419237	27.8267187465041	22.209255907556326
44	22.050284441168195	27.99770139035267	27.11593965678649	22.836074511692644
45	22.662147951799852	28.574473421737167	27.471329201645293	21.292049424817684
46	21.115414462164203	28.802921306769	28.375111427222272	21.70655280384452
47	21.65191372206371	29.124985722007185	27.907735316041165	21.315365239887942
48	21.486465640378285	28.194708252032186	28.673389000823118	21.645437106766412
49	21.486112370452975	29.077529795374286	27.729216247119375	21.707141587053364
50	21.39708834927562	28.375346940505814	28.35803671416577	21.8695279960528
51	21.76095637234179	27.179881513267052	28.844018374746383	22.215143739644773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6088.0
1	4707.5
2	3327.0
3	2233.0
4	1139.0
5	1130.5
6	1122.0
7	1194.5
8	1267.0
9	1463.5
10	1660.0
11	1956.5
12	2253.0
13	2386.0
14	2519.0
15	2693.0
16	2867.0
17	2839.5
18	2812.0
19	2931.0
20	3050.0
21	3318.5
22	3587.0
23	4865.0
24	6143.0
25	6574.5
26	8979.5
27	10953.0
28	13853.0
29	16753.0
30	18335.5
31	19918.0
32	21715.5
33	23513.0
34	26599.0
35	29685.0
36	33299.0
37	36913.0
38	38837.0
39	40761.0
40	41897.0
41	43033.0
42	44727.0
43	46421.0
44	51554.5
45	56688.0
46	60311.0
47	63934.0
48	62259.5
49	60585.0
50	57574.0
51	54563.0
52	51299.0
53	48035.0
54	44924.0
55	41813.0
56	39413.0
57	37013.0
58	35697.0
59	34381.0
60	33881.0
61	33381.0
62	31149.5
63	28918.0
64	25230.0
65	21542.0
66	18727.0
67	15912.0
68	13899.0
69	11886.0
70	10485.5
71	9085.0
72	8062.0
73	7039.0
74	5906.0
75	3913.0
76	3053.0
77	2454.5
78	1856.0
79	1401.5
80	947.0
81	714.0
82	481.0
83	371.5
84	262.0
85	211.0
86	160.0
87	106.5
88	53.0
89	39.5
90	26.0
91	22.5
92	19.0
93	13.0
94	7.0
95	6.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	849209.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.848054782802837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.08141469963483	17.666976361444274
2	8.355082495425235	3.9850493013147616
3	3.2566631179550374	2.3299524133837566
4	1.7760527871540717	1.6942161666079987
5	1.2289865929862542	1.4654469798433203
6	0.9259240392639183	1.3248892327848008
7	0.7648296180300366	1.2767789041203337
8	0.6300582780310006	1.2020531464673379
9	0.566403425285995	1.2156857923848823
>10	7.093759269518992	37.126655139669815
>50	0.9313832808731014	15.522113950271374
>100	0.3839782805937497	13.479113284855984
>500	0.0029804264987871904	0.5348572425044362
>1k	0.0019869509991914604	0.5772667425745553
>5k	4.967377497978651E-4	0.5989453417724097
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5056	0.5953775807840002	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1659	0.19535826869475006	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	1146	0.13494911146725955	TruSeq Adapter, Index 21 (95% over 22bp)
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1067	0.12564633676750953	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1001	0.11787439841075635	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	909	0.10704078736800952	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	874	0.10291930490609497	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1775664176898737E-4	0.0	0.0	0.16062005937289878	0.0
2	1.1775664176898737E-4	0.0	0.0	0.24093008905934818	0.0
3	1.1775664176898737E-4	0.0	0.0	0.3144102335231963	0.0
4	1.1775664176898737E-4	0.0	0.0	0.6223438517490982	0.0
5	1.1775664176898737E-4	0.0	0.0	0.6854614117372755	0.0
6	1.1775664176898737E-4	0.0	0.0	1.1126825080751617	0.0
7	1.1775664176898737E-4	0.0	0.0	1.3963582580966523	0.0
8	1.1775664176898737E-4	0.0	0.0	1.7283142312434276	0.0
9	1.1775664176898737E-4	0.0	0.0	2.098070086398048	0.0
10	1.1775664176898737E-4	0.0	0.0	2.7259485003102886	0.0
11	1.1775664176898737E-4	0.0	0.0	3.226060957903178	0.0
12	1.1775664176898737E-4	0.0	0.0	3.4751162552445862	0.0
13	1.1775664176898737E-4	0.0	0.0	3.591106547387039	0.0
14	1.1775664176898737E-4	0.0	0.0	3.7246425791530706	0.0
15	1.1775664176898737E-4	0.0	0.0	3.8461674334586657	0.0
16	1.1775664176898737E-4	0.0	0.0	4.063546194164216	0.0
17	1.1775664176898737E-4	0.0	0.0	4.319431376728226	0.0
18	1.1775664176898737E-4	0.0	0.0	4.620417353089757	0.0
19	1.1775664176898737E-4	0.0	0.0	4.769732774852834	0.0
20	1.1775664176898737E-4	0.0	0.0	4.942599524969707	0.0
21	1.1775664176898737E-4	0.0	0.0	5.132423231501315	0.0
22	1.1775664176898737E-4	0.0	0.0	5.3422655671336505	0.0
23	1.1775664176898737E-4	0.0	0.0	5.572715315075558	0.0
24	1.1775664176898737E-4	0.0	0.0	5.749468034370809	0.0
25	1.1775664176898737E-4	0.0	0.0	5.9123254699373184	0.0
26	1.1775664176898737E-4	0.0	0.0	6.064702564386388	0.0
27	1.1775664176898737E-4	0.0	0.0	6.22379178741629	0.0
28	1.1775664176898737E-4	0.0	0.0	6.379466067834891	0.0
29	1.1775664176898737E-4	0.0	0.0	6.547740308922774	0.0
30	1.1775664176898737E-4	0.0	0.0	6.762881693434714	0.0
31	1.1775664176898737E-4	0.0	0.0	6.936337226760433	0.0
32	1.1775664176898737E-4	0.0	0.0	7.1254543934414265	0.0
33	1.1775664176898737E-4	0.0	0.0	7.311156617511119	0.0
34	1.1775664176898737E-4	0.0	0.0	7.491088766134132	0.0
35	1.1775664176898737E-4	0.0	0.0	7.687389087963034	0.0
36	1.1775664176898737E-4	0.0	0.0	7.882276330090708	0.0
37	1.1775664176898737E-4	0.0	0.0	8.072453306547622	0.0
38	1.1775664176898737E-4	0.0	0.0	8.289714310611405	0.0
39	1.1775664176898737E-4	0.0	0.0	8.504737938481576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTT	25	3.8899423E-5	45.0	44
ATTCACG	20	7.0322456E-4	45.0	1
CATAGCG	40	6.8121153E-9	45.0	1
ACGCTAG	25	3.8899423E-5	45.0	1
CCGTTAA	20	7.0322456E-4	45.0	43
AATCGAG	20	7.0322456E-4	45.0	1
TGCGTAT	20	7.0322456E-4	45.0	43
ATCGTAC	30	2.1648302E-6	44.999996	45
ATTCCCG	30	2.1648302E-6	44.999996	1
CGTACAC	30	2.1648302E-6	44.999996	36
CTCGATA	30	2.1648302E-6	44.999996	24
AGTTACG	30	2.1648302E-6	44.999996	1
GCCCGTA	30	2.1648302E-6	44.999996	12
TTACCGG	135	0.0	41.666664	2
ACTACCG	55	6.002665E-11	40.909092	1
TAGTACG	45	1.9272193E-8	40.0	1
GTAGGGA	350	0.0	39.857143	4
TATGGGC	130	0.0	39.80769	4
TGCGAAG	130	0.0	39.80769	1
CGCATAG	40	3.4568075E-7	39.375	1
>>END_MODULE