FastQCFastQC Report
Sat 18 Jun 2016
SRR3554113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837300
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34290.40953063418129704No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15170.18117759464946853No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14590.17425056729965363No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA12040.14379553326167444No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT10990.13125522512838886No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9800.11704287591066524No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8960.1070106294040368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG351.2113924E-745.0000041
CTATCGG502.1827873E-1145.02
CCGATAG207.032189E-445.01
CGTTGTA207.032189E-445.021
CGCGAAG207.032189E-445.01
TCTACCG253.8898947E-545.01
ATTCACG207.032189E-445.01
ACGTCTG207.032189E-445.01
TTACGCG253.8898947E-545.01
CACGTTA253.8898947E-545.025
ATACGTT207.032189E-445.025
TAATGCG406.8121153E-945.01
CCGTTGC207.032189E-445.031
ATCGCGT207.032189E-445.020
AAATGCG253.8898947E-545.01
AGCCCGT302.1647938E-644.99999641
ATACCCG302.1647938E-644.9999961
TACAACG556.002665E-1140.9090921
TTAACGG1050.040.7142872
ACAATCG501.0804797E-940.51