FastQCFastQC Report
Sat 18 Jun 2016
SRR3554107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838637
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46900.5592407680557857No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA12360.14738200198655677No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12110.14440097443828498No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9610.11459069895556719No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8930.10648230402426795No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8710.1038589997817888No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG351.2114106E-745.0000041
TCGCACT207.032195E-445.024
CGAGTTA207.032195E-445.044
CGCATTA207.032195E-445.036
ACCGACG406.8121153E-945.01
CACGATT253.889901E-545.016
ATCGTAA207.032195E-445.024
CATAGCG453.8380676E-1045.01
GAACGAT253.889901E-545.027
CGGTCTA207.032195E-445.031
TCGAAAT207.032195E-445.016
GTACGTA207.032195E-445.025
CGCTTAA253.889901E-545.020
CGAATAG302.1647975E-644.9999961
ATTTCGC302.1647975E-644.99999619
ATTGCGG650.041.5384642
TCGCATA556.002665E-1140.90909210
TAACACG556.002665E-1140.9090921
TTAACGG1100.040.9090922
CTTAACG451.9272193E-840.01