##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	838637
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31864441945681	34.0	31.0	34.0	30.0	34.0
2	32.48835670260196	34.0	31.0	34.0	30.0	34.0
3	32.57805939876251	34.0	31.0	34.0	31.0	34.0
4	36.087570665258035	37.0	35.0	37.0	35.0	37.0
5	35.93262162294294	37.0	35.0	37.0	35.0	37.0
6	35.90902738610388	37.0	35.0	37.0	35.0	37.0
7	35.92589523238302	37.0	35.0	37.0	35.0	37.0
8	36.03537645012085	37.0	35.0	37.0	35.0	37.0
9	37.64558086514189	39.0	38.0	39.0	35.0	39.0
10	37.28068759189017	39.0	37.0	39.0	34.0	39.0
11	37.23034757588802	39.0	37.0	39.0	34.0	39.0
12	37.160905135356536	39.0	37.0	39.0	34.0	39.0
13	37.124441206386074	39.0	37.0	39.0	33.0	39.0
14	38.3817885449843	40.0	38.0	41.0	34.0	41.0
15	38.39294355006994	40.0	38.0	41.0	34.0	41.0
16	38.378344861960535	40.0	38.0	41.0	34.0	41.0
17	38.34456385778352	40.0	38.0	41.0	34.0	41.0
18	38.271253235905405	40.0	37.0	41.0	34.0	41.0
19	38.251120568255395	40.0	37.0	41.0	34.0	41.0
20	38.162101123608906	40.0	37.0	41.0	34.0	41.0
21	38.107194173402796	40.0	37.0	41.0	33.0	41.0
22	38.03426035340678	40.0	37.0	41.0	33.0	41.0
23	37.944353754961924	40.0	37.0	41.0	33.0	41.0
24	37.91266781694583	40.0	37.0	41.0	33.0	41.0
25	37.83501443413539	40.0	37.0	41.0	33.0	41.0
26	37.77391648591703	40.0	36.0	41.0	33.0	41.0
27	37.69676153091266	40.0	36.0	41.0	33.0	41.0
28	37.550378769360286	40.0	36.0	41.0	33.0	41.0
29	37.473428909051236	40.0	36.0	41.0	32.0	41.0
30	37.42158168552067	40.0	36.0	41.0	32.0	41.0
31	37.34018651693164	40.0	36.0	41.0	32.0	41.0
32	37.28688574436854	39.0	36.0	41.0	32.0	41.0
33	37.20659594079441	39.0	35.0	41.0	32.0	41.0
34	37.084809041337316	39.0	35.0	41.0	31.0	41.0
35	37.001073169917376	39.0	35.0	41.0	31.0	41.0
36	36.922748459703065	39.0	35.0	41.0	31.0	41.0
37	36.83973399694981	39.0	35.0	41.0	31.0	41.0
38	36.750756286689	39.0	35.0	41.0	31.0	41.0
39	36.67479731993699	39.0	35.0	41.0	31.0	41.0
40	36.54441313703068	39.0	35.0	41.0	30.0	41.0
41	36.43879294617337	39.0	35.0	41.0	30.0	41.0
42	36.33459053201803	39.0	35.0	40.0	30.0	41.0
43	36.24029943825517	39.0	35.0	40.0	30.0	41.0
44	36.130001418969115	38.0	35.0	40.0	30.0	41.0
45	35.99294331158773	38.0	35.0	40.0	29.0	41.0
46	35.84831458664476	38.0	35.0	40.0	29.0	41.0
47	35.70544943759934	38.0	34.0	40.0	28.0	41.0
48	35.59330675846642	38.0	34.0	40.0	28.0	41.0
49	35.4774652203516	38.0	34.0	40.0	28.0	41.0
50	35.382211850896155	38.0	34.0	40.0	28.0	41.0
51	34.30759911618495	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	2.0
9	6.0
10	8.0
11	6.0
12	14.0
13	18.0
14	29.0
15	47.0
16	82.0
17	156.0
18	282.0
19	459.0
20	746.0
21	1296.0
22	1814.0
23	2569.0
24	3509.0
25	4452.0
26	5732.0
27	6947.0
28	8748.0
29	10980.0
30	14084.0
31	17812.0
32	23334.0
33	31557.0
34	49472.0
35	62810.0
36	73206.0
37	104098.0
38	169552.0
39	244722.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.605847345156484	31.36076753112491	18.207520059334374	27.82586506438423
2	23.843450742096998	30.783163633371768	18.999877181665013	26.37350844286622
3	21.997836966410976	30.670719274250956	20.08914464780352	27.24229911153455
4	22.16453602691033	31.223640263904407	18.812668651633544	27.799155057551715
5	19.583323893412764	33.44712909160936	16.752778615777743	30.216768399200127
6	20.932894685066366	37.17699076000701	19.498781952143776	22.39133260278285
7	84.06294976253135	6.717208995071765	5.027324098507459	4.19251714388943
8	85.30281874040855	6.122911343048303	4.803627791285145	3.770642125258008
9	76.09728643024336	10.255092489360711	7.844633613828152	5.802987466567775
10	36.1166988816377	33.07128113832326	15.0986660497927	15.71335393024634
11	26.720261567281195	30.002372897928424	24.454799871696576	18.82256566309381
12	26.60173591196191	25.671059111391457	28.540238506052084	19.186966470594548
13	24.48902206795073	26.631069223036903	27.96418474262404	20.91572396638832
14	21.215376855540597	28.49349599409518	28.46177786098157	21.82934928938265
15	21.252341597139168	29.225874842154592	28.796726116305386	20.725057444400854
16	22.37118085655653	28.053257845766407	28.460227726656466	21.115333571020596
17	22.126498115394384	28.099642634417517	28.065062714857557	21.708796535330542
18	23.41668683828641	27.642949214022277	27.06129111880349	21.87907282888783
19	23.117868636847646	28.353506940428336	26.89351888838675	21.635105534337264
20	24.218821730975378	28.14757755739372	26.558093668655207	21.075507042975687
21	24.31719564006835	28.44281852577456	26.473670968488154	20.766314865668935
22	22.924698051719634	27.611350322010598	26.24544349939247	23.218508126877303
23	22.14951164806704	28.50756644412302	26.577649209371874	22.76527269843806
24	21.095897271405864	27.940813486645595	28.385940520153536	22.57734872179501
25	22.03730577115009	29.757690156766277	25.835015626546408	22.369988445537224
26	22.606801273971932	28.473582730072728	26.335947495758	22.583668500197344
27	21.432634143258646	28.157832292159778	26.881594778193662	23.527938786387914
28	21.8948126543427	29.419045427282605	27.633767649173603	21.052374269201096
29	21.6978263539529	28.937788339889607	26.435036851462552	22.92934845469494
30	22.140330083218366	28.450330715196202	27.653442430992193	21.755896770593235
31	22.39979872101994	29.165538844577572	26.105931410133348	22.328731024269143
32	22.87759781645694	28.45653125249661	26.802299445409634	21.86357148563681
33	21.975658121451833	28.122059961580515	27.136413013019933	22.765868903947712
34	21.181035418184507	28.835240992229057	28.45653125249661	21.527192337089826
35	22.597381226919396	28.03310609954009	28.064824232653702	21.30468844088682
36	22.357468129834484	30.313592173967997	26.82221270943209	20.50672698676543
37	21.354888944799715	30.566264068959516	27.308477923106185	20.770369063134588
38	21.95884512607958	28.454861877069575	28.77108927939025	20.81520371746059
39	22.26982591991529	28.182515200259466	26.885887457863177	22.661771421962065
40	22.608947613806688	28.483360500431054	28.094276784830623	20.813415100931632
41	21.255441865789372	27.508922215451975	29.168758354329704	22.06687756442895
42	21.88813515263457	28.631457949029198	27.430700052585326	22.0497068457509
43	21.92998877941231	27.308358682004254	28.854081086334133	21.907571452249304
44	22.660698252044686	27.39325834657903	27.37942637875505	22.56661702262123
45	21.918780115830806	28.346113992108624	28.247143877506005	21.48796201455457
46	21.09673195911938	28.087718524224424	28.168206268027763	22.64734324862843
47	21.239225075926772	28.903923866941238	28.296748175909244	21.560102881222747
48	21.275235888709894	27.790927421518486	29.152660805569035	21.78117588420258
49	20.880547841318712	29.07586953592556	28.48968027883339	21.553902343922342
50	21.15969126093888	27.767317683336174	28.890926586830773	22.18206446889417
51	21.402108421164343	27.496282658647303	28.16236345403315	22.939245466155203
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5711.0
1	4622.0
2	3533.0
3	2498.5
4	1464.0
5	1574.0
6	1684.0
7	1808.0
8	1932.0
9	2035.5
10	2139.0
11	2412.5
12	2686.0
13	2996.0
14	3306.0
15	3318.5
16	3331.0
17	3542.5
18	3754.0
19	3652.0
20	3550.0
21	3868.5
22	4187.0
23	4903.5
24	5620.0
25	6447.5
26	8953.5
27	10632.0
28	12303.5
29	13975.0
30	16365.5
31	18756.0
32	22052.5
33	25349.0
34	27812.0
35	30275.0
36	32866.5
37	35458.0
38	36628.0
39	37798.0
40	39323.5
41	40849.0
42	42274.0
43	43699.0
44	46897.0
45	50095.0
46	53479.5
47	56864.0
48	58559.0
49	60254.0
50	57784.0
51	55314.0
52	53460.5
53	51607.0
54	47344.5
55	43082.0
56	41133.0
57	39184.0
58	37483.0
59	35782.0
60	34092.0
61	32402.0
62	30343.5
63	28285.0
64	24536.0
65	20787.0
66	18141.0
67	15495.0
68	14328.5
69	13162.0
70	11353.0
71	9544.0
72	8251.5
73	6959.0
74	6081.5
75	4297.5
76	3391.0
77	2672.0
78	1953.0
79	1515.0
80	1077.0
81	848.5
82	620.0
83	455.5
84	291.0
85	218.5
86	146.0
87	116.0
88	86.0
89	61.5
90	37.0
91	26.0
92	15.0
93	16.0
94	17.0
95	14.5
96	12.0
97	8.0
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	838637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.702302829593947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19933049470596	18.081920288037576
2	8.292067524634888	4.096663261539449
3	3.286782700660344	2.4357330482034736
4	1.9356567313764808	1.9126071501041526
5	1.3263051883329902	1.638139620333158
6	0.9829162906339454	1.4568177520428538
7	0.8251532021762606	1.4268228996677006
8	0.6843193876600181	1.352341179691205
9	0.6107817563998242	1.3578944318441764
>10	7.716014581746568	40.67972362689783
>50	0.8951680567380301	15.322851260611117
>100	0.23919666773286807	8.525679122197861
>500	0.0048518593860622326	0.8574219222633511
>1k	0.0014555578158186697	0.8553844365660522
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4690	0.5592407680557857	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1236	0.14738200198655677	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1211	0.14440097443828498	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	961	0.11459069895556719	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	893	0.10648230402426795	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	871	0.1038589997817888	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3848220386174233E-4	0.0	0.0	0.10481292859723575	0.0
2	2.3848220386174233E-4	0.0	0.0	0.174926696532588	0.0
3	2.3848220386174233E-4	0.0	0.0	0.24539818777373285	0.0
4	2.3848220386174233E-4	0.0	0.0	0.5338424133445102	0.0
5	3.577233057926135E-4	0.0	0.0	0.6119453351092308	0.0
6	3.577233057926135E-4	0.0	0.0	1.0997606831084248	0.0
7	3.577233057926135E-4	0.0	0.0	1.4536682736392503	0.0
8	3.577233057926135E-4	0.0	0.0	1.8053102832333894	0.0
9	3.577233057926135E-4	0.0	0.0	2.190578283572034	0.0
10	3.577233057926135E-4	0.0	0.0	2.872637386616617	0.0
11	3.577233057926135E-4	0.0	0.0	3.3823930973710916	0.0
12	3.577233057926135E-4	0.0	0.0	3.633634099139437	0.0
13	3.577233057926135E-4	0.0	0.0	3.759433461676506	0.0
14	3.577233057926135E-4	0.0	0.0	3.891075638208188	0.0
15	3.577233057926135E-4	0.0	0.0	3.9975579422324556	0.0
16	3.577233057926135E-4	0.0	0.0	4.180593033696343	0.0
17	3.577233057926135E-4	0.0	0.0	4.397969562516321	0.0
18	3.577233057926135E-4	0.0	0.0	4.6432485091881235	0.0
19	3.577233057926135E-4	0.0	0.0	4.774652203515943	0.0
20	3.577233057926135E-4	0.0	0.0	4.9142835338769935	0.0
21	3.577233057926135E-4	0.0	0.0	5.078836254541596	0.0
22	3.577233057926135E-4	0.0	0.0	5.250662682423981	0.0
23	3.577233057926135E-4	0.0	0.0	5.419150359452302	0.0
24	3.577233057926135E-4	0.0	0.0	5.562835887279001	0.0
25	3.577233057926135E-4	0.0	0.0	5.694835787116476	0.0
26	3.577233057926135E-4	0.0	0.0	5.824570106017264	0.0
27	3.577233057926135E-4	0.0	0.0	5.965393847397623	0.0
28	3.577233057926135E-4	0.0	0.0	6.097989952744752	0.0
29	3.577233057926135E-4	0.0	0.0	6.235713425474907	0.0
30	3.577233057926135E-4	0.0	0.0	6.43448834239367	0.0
31	5.962055096543559E-4	0.0	0.0	6.587355435069046	0.0
32	5.962055096543559E-4	0.0	0.0	6.747257752758345	0.0
33	5.962055096543559E-4	0.0	0.0	6.914672259869288	0.0
34	5.962055096543559E-4	0.0	0.0	7.071593550010315	0.0
35	5.962055096543559E-4	0.0	0.0	7.244016183402354	0.0
36	5.962055096543559E-4	0.0	0.0	7.4037992599897215	0.0
37	5.962055096543559E-4	0.0	0.0	7.562151443353918	0.0
38	7.15446611585227E-4	0.0	0.0	7.732308495809272	0.0
39	7.15446611585227E-4	0.0	0.0	7.909143049972753	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	35	1.2114106E-7	45.000004	1
TCGCACT	20	7.032195E-4	45.0	24
CGAGTTA	20	7.032195E-4	45.0	44
CGCATTA	20	7.032195E-4	45.0	36
ACCGACG	40	6.8121153E-9	45.0	1
CACGATT	25	3.889901E-5	45.0	16
ATCGTAA	20	7.032195E-4	45.0	24
CATAGCG	45	3.8380676E-10	45.0	1
GAACGAT	25	3.889901E-5	45.0	27
CGGTCTA	20	7.032195E-4	45.0	31
TCGAAAT	20	7.032195E-4	45.0	16
GTACGTA	20	7.032195E-4	45.0	25
CGCTTAA	25	3.889901E-5	45.0	20
CGAATAG	30	2.1647975E-6	44.999996	1
ATTTCGC	30	2.1647975E-6	44.999996	19
ATTGCGG	65	0.0	41.538464	2
TCGCATA	55	6.002665E-11	40.909092	10
TAACACG	55	6.002665E-11	40.909092	1
TTAACGG	110	0.0	40.909092	2
CTTAACG	45	1.9272193E-8	40.0	1
>>END_MODULE