##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554106_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833773
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26270939452345	34.0	31.0	34.0	30.0	34.0
2	32.444301986272045	34.0	31.0	34.0	30.0	34.0
3	32.53023424841054	34.0	31.0	34.0	30.0	34.0
4	36.06865177932123	37.0	35.0	37.0	35.0	37.0
5	35.90398105959296	37.0	35.0	37.0	35.0	37.0
6	35.86365833386306	37.0	35.0	37.0	35.0	37.0
7	35.94431937709664	37.0	35.0	37.0	35.0	37.0
8	36.044159501447034	37.0	35.0	37.0	35.0	37.0
9	37.694313680102375	39.0	38.0	39.0	35.0	39.0
10	37.26425537886211	39.0	37.0	39.0	34.0	39.0
11	37.20471159416292	39.0	37.0	39.0	34.0	39.0
12	37.1505577657228	39.0	37.0	39.0	34.0	39.0
13	37.13344879241712	39.0	37.0	39.0	33.0	39.0
14	38.37825523253931	40.0	38.0	41.0	34.0	41.0
15	38.38206562217774	40.0	38.0	41.0	34.0	41.0
16	38.374786662556836	40.0	38.0	41.0	34.0	41.0
17	38.331977648592606	40.0	38.0	41.0	34.0	41.0
18	38.26060810316477	40.0	37.0	41.0	34.0	41.0
19	38.242721939904506	40.0	37.0	41.0	34.0	41.0
20	38.153673721744404	40.0	37.0	41.0	33.0	41.0
21	38.127243266452616	40.0	37.0	41.0	33.0	41.0
22	38.047055973268506	40.0	37.0	41.0	33.0	41.0
23	37.99074328384344	40.0	37.0	41.0	33.0	41.0
24	37.90113136309283	40.0	37.0	41.0	33.0	41.0
25	37.84299923360435	40.0	37.0	41.0	33.0	41.0
26	37.70504441856477	40.0	37.0	41.0	33.0	41.0
27	37.6479953176704	40.0	36.0	41.0	33.0	41.0
28	37.566462334472334	40.0	36.0	41.0	33.0	41.0
29	37.45446182594063	40.0	36.0	41.0	32.0	41.0
30	37.422916069481744	40.0	36.0	41.0	32.0	41.0
31	37.36126739532223	40.0	36.0	41.0	32.0	41.0
32	37.301994667613364	39.0	36.0	41.0	32.0	41.0
33	37.21002119281867	39.0	36.0	41.0	32.0	41.0
34	37.10685522318425	39.0	35.0	41.0	31.0	41.0
35	37.06786979189779	39.0	35.0	41.0	31.0	41.0
36	36.9367861516264	39.0	35.0	41.0	31.0	41.0
37	36.8256503868559	39.0	35.0	41.0	31.0	41.0
38	36.753678759086704	39.0	35.0	41.0	31.0	41.0
39	36.69366722117411	39.0	35.0	41.0	31.0	41.0
40	36.59255696694424	39.0	35.0	41.0	30.0	41.0
41	36.49215194063612	39.0	35.0	41.0	30.0	41.0
42	36.3958055729797	39.0	35.0	40.0	30.0	41.0
43	36.324428831348584	39.0	35.0	40.0	30.0	41.0
44	36.20677450577075	39.0	35.0	40.0	30.0	41.0
45	36.069902719325285	38.0	35.0	40.0	29.0	41.0
46	35.96749714850445	38.0	35.0	40.0	29.0	41.0
47	35.80496130241684	38.0	35.0	40.0	28.0	41.0
48	35.70763145364506	38.0	34.0	40.0	28.0	41.0
49	35.53806011948096	38.0	34.0	40.0	28.0	41.0
50	35.4156335117592	38.0	34.0	40.0	27.0	41.0
51	34.321317672795836	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	6.0
11	10.0
12	11.0
13	18.0
14	32.0
15	42.0
16	76.0
17	131.0
18	282.0
19	512.0
20	767.0
21	1267.0
22	1790.0
23	2553.0
24	3365.0
25	4631.0
26	5576.0
27	6824.0
28	8759.0
29	10984.0
30	13994.0
31	18097.0
32	23185.0
33	31177.0
34	47581.0
35	60913.0
36	72150.0
37	103524.0
38	170488.0
39	244963.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.89768678045463	31.581497601865255	19.513704569469148	27.007111048210962
2	24.61149497525106	30.072213899946387	19.59730046427505	25.718990660527506
3	21.28457026073044	30.606412056998728	20.604049303587427	27.504968378683408
4	21.77535132464112	31.647942545512986	19.636879582332362	26.939826547513533
5	18.930332356648634	33.78581460421481	17.046846084006077	30.237006955130475
6	20.418027448718057	38.528112567809224	19.547526724899946	21.506333258572777
7	85.72357224328444	5.9425047345020765	4.419068499459685	3.9148545227537954
8	86.53386473296689	5.5817350765736	4.197185564895961	3.687214625563553
9	78.17103696089943	10.045899783274344	6.877051667540206	4.90601158828602
10	33.16514207104332	38.079789103269114	13.697373265864929	15.057695559822637
11	25.025156727310673	30.326239875841505	26.58265499122663	18.065948405621192
12	26.23699736019276	25.939554291155986	29.053351451774045	18.77009689687721
13	24.630205103787244	26.50649517314665	28.43315866548809	20.430141057578023
14	21.19581708690495	28.691502363353095	28.342006757234884	21.770673792507072
15	20.827851225693326	30.55100129171849	28.89875301790775	19.722394464680434
16	22.147275097658476	28.216073199779796	28.133076988580825	21.503574713980903
17	21.43832913754703	28.75446914208064	28.577322604593817	21.229879115778516
18	22.259415932154194	28.261049470299472	28.28923459982513	21.1902999977212
19	22.10889534681502	29.215146088923483	27.35732627465749	21.318632289604007
20	23.49452428898513	28.15430578826611	27.84378961659828	20.507380306150473
21	23.855174010192222	28.05619755017253	26.949661358667164	21.138967080968083
22	22.361841892217665	28.210076363710506	27.171424356509505	22.256657387562324
23	21.848992471572	29.280391665357357	27.07991263809214	21.790703224978504
24	20.9547442769195	29.015691321258902	29.055990059644532	20.973574342177066
25	21.982362105753005	30.277785440401644	25.74309794152605	21.9967545123193
26	22.71613496719131	29.45693851923725	26.230640713959314	21.596285799612126
27	21.936426341462244	28.71273116303838	26.755603743464945	22.595238752034426
28	21.412422805727697	30.176079100666485	28.08210388199186	20.329394211613952
29	21.74020986527508	28.97731157041545	26.969330980974437	22.313147583335034
30	22.5169200729695	28.852337506731445	27.72145416078477	20.90928825951428
31	22.46786595392271	29.591267647189344	26.642023668312596	21.29884273057535
32	22.04125103595343	28.72424508829142	27.75287758178785	21.481626293967302
33	21.265260448587327	29.579154038329374	27.20296771423397	21.952617798849328
34	20.960621176267402	28.75926661093607	28.819354908350352	21.460757304446172
35	22.424328924059665	27.75347726539478	28.47549632813727	21.34669748240828
36	22.544505518888236	31.12333932617151	25.971337522323225	20.36081763261703
37	20.966618012336692	31.035545646117107	27.314628801844147	20.683207539702053
38	20.6692948800213	29.305578376848374	28.953204289416906	21.07192245371342
39	20.660059752474595	28.992303660588675	27.587844653160992	22.759791933775738
40	21.827403861722555	29.18072424988576	28.733959962723667	20.25791192566802
41	20.43529833659761	29.041957463242397	29.322489454563772	21.200254745596222
42	21.37728134636166	29.60506037014871	27.46035191832789	21.55730636516174
43	21.363488623402294	29.401048007071466	28.060515272142418	21.17494809738382
44	21.54591237663009	28.746793191911944	27.981596909470564	21.7256975219874
45	22.25593776723401	29.38845465132596	27.18737594045382	21.168231640986214
46	19.97845936483911	29.27067679092511	28.824392250648557	21.926471593587223
47	20.55775372913251	29.888111032619193	28.611864380352927	20.942270857895373
48	20.503062584180586	28.89995238512161	28.866370103133587	21.73061492756422
49	20.420306246424385	30.392684819489237	27.812965879202135	21.374043054884243
50	20.374130608690855	28.684306160069944	28.805921995555146	22.13564123568405
51	21.083556315687844	27.295199052979648	29.097248291801247	22.523996339531262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5769.0
1	4518.0
2	3267.0
3	2196.5
4	1126.0
5	1113.0
6	1100.0
7	1259.0
8	1418.0
9	1625.0
10	1832.0
11	2069.0
12	2306.0
13	2378.5
14	2451.0
15	2916.5
16	3382.0
17	3250.0
18	3118.0
19	3262.5
20	3407.0
21	3844.0
22	4281.0
23	5615.0
24	6949.0
25	7298.0
26	9048.0
27	10449.0
28	12890.0
29	15331.0
30	20236.5
31	25142.0
32	27048.0
33	28954.0
34	31526.0
35	34098.0
36	36355.0
37	38612.0
38	39962.5
39	41313.0
40	43098.5
41	44884.0
42	45778.5
43	46673.0
44	50511.5
45	54350.0
46	56495.5
47	58641.0
48	58578.5
49	58516.0
50	56821.0
51	55126.0
52	50817.0
53	46508.0
54	43288.0
55	40068.0
56	37852.5
57	35637.0
58	32885.0
59	30133.0
60	29585.5
61	29038.0
62	27080.0
63	25122.0
64	21604.5
65	18087.0
66	16200.5
67	14314.0
68	12091.0
69	9868.0
70	8834.5
71	7801.0
72	6831.5
73	5862.0
74	5197.5
75	3810.0
76	3087.0
77	2414.0
78	1741.0
79	1304.0
80	867.0
81	636.5
82	406.0
83	313.5
84	221.0
85	173.0
86	125.0
87	107.0
88	89.0
89	78.5
90	68.0
91	50.5
92	33.0
93	22.0
94	11.0
95	7.0
96	3.0
97	4.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	833773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.043880565251683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86016960501537	17.75885096512222
2	8.36882498949012	4.0243805703758815
3	3.236029355805993	2.334201100099429
4	1.8744925246924928	1.8028029753665353
5	1.2805220122697893	1.539435916209528
6	0.9816770193415728	1.4161995004020624
7	0.7752624146024759	1.3048221832401392
8	0.6550110381132478	1.2599205735453156
9	0.5730679528767786	1.2400899673268286
>10	7.078638517627415	37.43963131111898
>50	0.9511103144182915	15.813197235934147
>100	0.35967621067340827	12.557106190647465
>500	0.004514764150712238	0.8064240898152815
>1k	0.0010032809223804973	0.70293742079621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4641	0.5566263239514831	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1187	0.14236488828494087	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	927	0.11118134072463368	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	913	0.10950222662523253	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	851	0.10206614989931313	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13097089975329015	0.0
2	0.0	0.0	0.0	0.19453736208776248	0.0
3	0.0	0.0	0.0	0.26182186278519454	0.0
4	0.0	0.0	0.0	0.554947209852082	0.0
5	0.0	0.0	0.0	0.6144358236594373	0.0
6	0.0	0.0	0.0	0.9875589638906513	0.0
7	0.0	0.0	0.0	1.2557374729092932	0.0
8	0.0	0.0	0.0	1.5567786435876432	0.0
9	0.0	0.0	0.0	1.9225856438143236	0.0
10	0.0	0.0	0.0	2.456783800866663	0.0
11	0.0	0.0	0.0	2.872484477189835	0.0
12	0.0	0.0	0.0	3.0727788019041156	0.0
13	0.0	0.0	0.0	3.172686090818484	0.0
14	0.0	0.0	0.0	3.2806291400657015	0.0
15	0.0	0.0	0.0	3.374059846025237	0.0
16	0.0	0.0	0.0	3.547128534984942	0.0
17	0.0	0.0	0.0	3.7582171646239444	0.0
18	0.0	0.0	0.0	4.012243140519062	0.0
19	0.0	0.0	0.0	4.134098849447032	0.0
20	0.0	0.0	0.0	4.264829875757551	0.0
21	0.0	0.0	0.0	4.410672928962679	0.0
22	0.0	0.0	0.0	4.579903642838039	0.0
23	0.0	0.0	0.0	4.756930243603475	0.0
24	0.0	0.0	0.0	4.903013170251375	0.0
25	0.0	0.0	0.0	5.027507487049832	0.0
26	0.0	0.0	0.0	5.142646739580197	0.0
27	0.0	0.0	0.0	5.273857512776259	0.0
28	0.0	0.0	0.0	5.4153828440115	0.0
29	0.0	0.0	0.0	5.566263239514832	0.0
30	0.0	0.0	0.0	5.759241424224579	0.0
31	0.0	0.0	0.0	5.901006628902591	0.0
32	0.0	0.0	0.0	6.054165822112253	0.0
33	0.0	0.0	0.0	6.201448115974012	0.0
34	0.0	0.0	0.0	6.353288005248431	0.0
35	0.0	0.0	0.0	6.523118402730719	0.0
36	0.0	0.0	0.0	6.680475381188884	0.0
37	0.0	0.0	0.0	6.835193751776563	0.0
38	0.0	0.0	0.0	7.002505478109749	0.0
39	0.0	0.0	0.0	7.178692521825485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCCG	30	2.164781E-6	45.000004	1
CGAGTAC	20	7.032171E-4	45.0	26
ACCGCGT	20	7.032171E-4	45.0	24
CATGACG	25	3.8898816E-5	45.0	1
TAACGCA	25	3.8898816E-5	45.0	39
TCTACCG	35	1.2113924E-7	45.0	1
CGAATCA	25	3.8898816E-5	45.0	12
CATAACG	40	6.8102963E-9	45.0	1
TAGATCG	20	7.032171E-4	45.0	1
CCGAACT	20	7.032171E-4	45.0	12
CGCGTAG	20	7.032171E-4	45.0	1
CAATACG	20	7.032171E-4	45.0	1
TAAGACG	20	7.032171E-4	45.0	1
CGATAGA	20	7.032171E-4	45.0	43
CCGCATT	20	7.032171E-4	45.0	23
GCGTAAT	20	7.032171E-4	45.0	25
GCGTAAC	20	7.032171E-4	45.0	32
TACGTAG	20	7.032171E-4	45.0	1
TACCGCG	70	0.0	45.0	1
TATCCCG	40	6.8102963E-9	45.0	1
>>END_MODULE