FastQCFastQC Report
Sat 18 Jun 2016
SRR3554105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816453
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44350.5432033442218964No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12270.15028421721764756No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9380.11488720110036954No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT9180.11243758060782434No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8800.10778330167198846No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT207.032084E-445.00000427
AATTTCG207.032084E-445.00000439
CGTATAT207.032084E-445.00000430
CGAAACG207.032084E-445.0000041
ACGTTAG302.1647265E-645.0000041
CGACACG302.1647265E-645.0000041
ACGATAG302.1647265E-645.0000041
CGTACAG302.1647265E-645.0000041
CCGACGG600.045.0000042
TATAGCG207.032084E-445.0000041
GCGTAAA207.032084E-445.0000041
ACGCATT253.88981E-545.040
TACGCAT253.88981E-545.039
TAAACGG1050.042.8571432
GTTACGG850.042.352942
AAGGGCG1600.042.1875045
AATTGCG650.041.538461
CGTAAGG1200.041.2500042
TATACGG603.6379788E-1241.2500042
TAACACG556.002665E-1140.9090921