FastQCFastQC Report
Sat 18 Jun 2016
SRR3554104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087218
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43030.3957807909729235No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA20620.1896583757811221No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11510.1058665327468824No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG302.1653923E-645.0000041
CGACTTA207.0331176E-445.027
TAGGACG207.0331176E-445.01
GTAACGC207.0331176E-445.022
ATGCCCG650.044.9999961
ATACGCG850.042.352941
ATATCCG650.041.538461
AGTACGG1950.041.538462
TGCGACG451.9281288E-840.01
ACGGGTA3350.039.6268655
CTCGTAG403.4579898E-739.3751
TGACGGG4000.039.3753
TAGTACG403.4579898E-739.3751
CGAGGGA6100.039.0983624
CGGTAGT1050.038.5714312
TCGAACG356.2479776E-638.5714261
AGCACGT356.2479776E-638.57142618
ATAGGGC3750.038.3999984
TAGGGCG1600.037.968755
TACGGGA5800.037.629314
Produced by FastQC (version 0.11.5)